FastQCFastQC Report
Thu 26 May 2016
SRR1513089_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513089_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121202
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC71780.3383930431896632No Hit
CCCATGTACTCTGCGTTGATACCAC57250.26989414492349145No Hit
GAGTACATGGGAAGCAGTGGTATCA30150.14213639247935841No Hit
GTATCAACGCAGAGTACTTTTTTTT23840.11238910768517096No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGA250.00603828218.99700513
CGGATTC250.00603828218.99700514
CGACGAC350.002172836816.28314814
ACACCGA508.722789E-515.1986796
TAGGACC6000.013.1433144
GGCGAGG4850.011.75266519
GTAGGAC11400.011.7509193
TACACCG658.025037E-411.6923955
CCAACGA1001.930308E-611.400084519
TGGCGAG10250.011.21471818
GACGTGA4500.011.1871257
GGACGTG7700.011.1029316
TTAGGAC6650.011.000863
GTCCTAC13050.010.715381
TATGGCG10300.010.698116
TGTAGGA13000.010.670822
ATGGCGA10950.010.67030617
AGGACCT9400.010.6117815
GTACTAG1355.579932E-810.5695921
GGACCTG8450.010.5671296
Produced by FastQC (version 0.11.5)