##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513089_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2121202
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.416679788157847	31.0	30.0	34.0	25.0	34.0
2	29.15740792248923	31.0	30.0	34.0	18.0	34.0
3	29.49684801353195	31.0	30.0	34.0	24.0	34.0
4	32.420220705053076	35.0	33.0	37.0	20.0	37.0
5	32.63129678361608	35.0	33.0	37.0	25.0	37.0
6	33.08755460347482	37.0	35.0	37.0	27.0	37.0
7	33.073563008143495	37.0	35.0	37.0	27.0	37.0
8	33.048647417831965	37.0	35.0	37.0	27.0	37.0
9	34.438871451186635	39.0	35.0	39.0	27.0	39.0
10	33.95520322911255	39.0	34.0	39.0	23.0	39.0
11	34.318203075426105	39.0	35.0	39.0	27.0	39.0
12	34.155046054076884	39.0	35.0	39.0	25.0	39.0
13	34.24129290845473	39.0	35.0	39.0	26.0	39.0
14	34.55698561475993	40.0	34.0	41.0	17.0	41.0
15	35.107499898642374	40.0	34.0	41.0	25.0	41.0
16	34.69043353721145	39.0	34.0	41.0	18.0	41.0
17	34.23933741340994	39.0	33.0	41.0	17.0	41.0
18	33.65455246600748	39.0	32.0	41.0	11.0	41.0
19	34.549137234454804	39.0	34.0	41.0	18.0	41.0
20	34.807407781059986	39.0	34.0	41.0	22.0	41.0
21	34.836946693431365	40.0	35.0	41.0	19.0	41.0
22	34.421562397169154	40.0	34.0	41.0	16.0	41.0
23	32.75057066700861	39.0	31.0	41.0	8.0	41.0
24	33.850821373919125	39.0	33.0	41.0	2.0	41.0
25	33.57973309472648	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6116199083730294
1101	2	0.9547653733986685
1101	3	0.7095406052883
1101	4	0.7237182881775936
1101	5	0.9474110306751626
1101	6	0.6373606326423769
1101	7	0.6409481364715361
1101	8	0.5735212640492051
1101	9	0.682642315033803
1101	10	0.9836208223633776
1101	11	0.6141278612224355
1101	12	0.800144414307276
1101	13	0.533512446133571
1101	14	-0.164851544386039
1101	15	0.3216295848800641
1101	16	1.0431448552089222
1101	17	1.4932168489277373
1101	18	-4.425453930503327
1101	19	-1.031555078174982
1101	20	-0.021940230192704746
1101	21	0.4084608685538811
1101	22	1.0336395764222033
1101	23	2.8139553047862123
1101	24	1.696324528465496
1101	25	1.974081219062647
1102	1	0.8519308659515374
1102	2	1.2481060469839136
1102	3	0.9357648265578504
1102	4	1.4636156808264147
1102	5	1.2722688358197018
1102	6	0.9108522919657887
1102	7	0.8473969262009504
1102	8	0.8657714116651576
1102	9	1.0180533841577102
1102	10	1.4188352904684152
1102	11	1.104995494615899
1102	12	1.2236126773285676
1102	13	1.0941013601907557
1102	14	1.9479297443387509
1102	15	1.4217522427720652
1102	16	1.5781528111529965
1102	17	1.4102491766705825
1102	18	1.6754961575857052
1102	19	1.473298978755821
1102	20	1.4029561383047593
1102	21	1.4357053968525975
1102	22	1.7694103299032662
1102	23	3.2785806365842234
1102	24	2.1208067313579875
1102	25	2.4266858371743254
1103	1	0.6363828659435562
1103	2	1.0103884342443017
1103	3	0.7134870684005534
1103	4	1.1020685627423816
1103	5	1.0226165173773154
1103	6	0.616181546899675
1103	7	0.61284107068483
1103	8	0.6022325820700232
1103	9	0.6550153756297874
1103	10	1.0096596898323185
1103	11	0.7546492097704203
1103	12	0.8304360151912391
1103	13	0.6373403755486038
1103	14	1.349634132209701
1103	15	0.9644756718200895
1103	16	1.1597217977585288
1103	17	1.6198433918879616
1103	18	1.583404740780864
1103	19	1.1173233453967484
1103	20	0.9704620294370372
1103	21	1.0790832853031844
1103	22	1.2621967107627228
1103	23	2.761967743334928
1103	24	1.6557714244703945
1103	25	1.8249217897419854
1104	1	0.5855265889757177
1104	2	0.964400772883522
1104	3	0.7991415408845555
1104	4	1.2945264430788015
1104	5	1.021539539223312
1104	6	0.60803796567955
1104	7	0.591215726195621
1104	8	0.4839361512708251
1104	9	0.6505282641625456
1104	10	1.0826734535337863
1104	11	0.6713128187182633
1104	12	0.7706092837207663
1104	13	0.7118428681679276
1104	14	1.495267660146176
1104	15	0.9565381643204134
1104	16	1.3361652311172776
1104	17	1.8068956550792095
1104	18	2.339137071050196
1104	19	1.5452727135398163
1104	20	1.0455952385443439
1104	21	1.0248538651557624
1104	22	1.4187550217912346
1104	23	2.8319995865840326
1104	24	1.8556748336120208
1104	25	1.9726128814666097
1105	1	0.5049366917727092
1105	2	0.9707549924654231
1105	3	0.6404030339162254
1105	4	0.8491347700570842
1105	5	0.8796223935827996
1105	6	0.537729215643239
1105	7	0.3670278791991777
1105	8	0.40642544403488756
1105	9	0.6206057226523143
1105	10	1.1238847174118334
1105	11	0.6835269775290698
1105	12	0.592965156502018
1105	13	0.5599222642057953
1105	14	1.384039523558009
1105	15	0.7247212578861095
1105	16	1.1477529153998276
1105	17	1.6692796461483823
1105	18	1.5720907933403083
1105	19	1.2260598788907302
1105	20	0.8746094663092308
1105	21	0.7741783438473178
1105	22	1.273809262810289
1105	23	2.6630228839735324
1105	24	1.742261188327717
1105	25	1.7478309521478295
1106	1	-0.5640047749102983
1106	2	-0.18186811674807046
1106	3	-0.5352401067912211
1106	4	-2.8164273591890563
1106	5	-0.6499615654817745
1106	6	-0.6163410375436129
1106	7	-0.6561610721377988
1106	8	-0.5913428944935362
1106	9	-0.5961122934071668
1106	10	-0.5012259082893351
1106	11	-0.44912750278663793
1106	12	-0.40145338636662586
1106	13	-0.5000099202358612
1106	14	0.2238717439239295
1106	15	-0.37978608979113915
1106	16	-1.3972212729763172
1106	17	-6.118927694896847
1106	18	-7.358749007975401
1106	19	-3.2606953177698763
1106	20	-1.639644441137861
1106	21	-1.3375130879719848
1106	22	-1.5652679050527496
1106	23	-5.218470248427085
1106	24	-2.3583270270370242
1106	25	-0.9842982642378146
1107	1	0.3446224566622007
1107	2	0.7269787960013332
1107	3	0.47501389376229497
1107	4	0.667775174909643
1107	5	0.7694363488080498
1107	6	0.28398144911457024
1107	7	0.22553696380629162
1107	8	0.31703611725973957
1107	9	0.3211654652342375
1107	10	0.7139255887615619
1107	11	0.3859443648153018
1107	12	0.3107012213962861
1107	13	0.373603051970818
1107	14	1.1882767563714225
1107	15	0.6337438814246497
1107	16	-5.77427182420351
1107	17	-1.185525603346207
1107	18	0.5470641807267498
1107	19	0.45526090262543306
1107	20	0.44485582400604073
1107	21	0.5060559944575758
1107	22	0.6303419225937645
1107	23	2.360720170022418
1107	24	1.4008393525840006
1107	25	1.1203496186926927
1108	1	0.3551200441025806
1108	2	0.5095633618486275
1108	3	0.3114486084708403
1108	4	0.8040622769397174
1108	5	0.6879631849389014
1108	6	0.33235216821361746
1108	7	0.28638447550433455
1108	8	0.2912699749112875
1108	9	0.3193843489389607
1108	10	0.7628832793933285
1108	11	0.33890639656731736
1108	12	0.41290609634637576
1108	13	0.19780479734133394
1108	14	0.9283350663255021
1108	15	0.45035596142224676
1108	16	0.808320429511852
1108	17	0.9947198304389389
1108	18	0.7418963621424766
1108	19	0.7870569429244512
1108	20	0.5086255673069999
1108	21	0.5716189189562542
1108	22	0.9183757559471601
1108	23	2.663806792935574
1108	24	1.37006992325108
1108	25	1.4923675185186482
1109	1	0.11486045591737337
1109	2	0.3902054347177746
1109	3	0.2743423972349568
1109	4	0.19401498632239367
1109	5	-0.1334750052038416
1109	6	-0.043057324146886344
1109	7	0.04086582049610854
1109	8	-0.12236986362813695
1109	9	-0.09728552396576617
1109	10	0.4471084208720697
1109	11	0.09530423550710765
1109	12	0.06577199920349841
1109	13	-0.06538636303246648
1109	14	-1.1881893827731034
1109	15	-0.4676543977935097
1109	16	-0.5858957400763956
1109	17	0.46421735438675427
1109	18	1.2752155880283595
1109	19	0.46557330784229833
1109	20	0.20912752478503904
1109	21	0.18236126622362292
1109	22	-0.019441347966740352
1109	23	-4.358829247745174
1109	24	-1.7041352503346516
1109	25	-0.42700451605965384
1110	1	0.3896002781415575
1110	2	0.7468637959972746
1110	3	0.6010252334914945
1110	4	1.0182955193808354
1110	5	0.7548569032471519
1110	6	0.44859603973218753
1110	7	0.3116608976666271
1110	8	0.3139936716511045
1110	9	0.27354777125712104
1110	10	0.7577878288025204
1110	11	0.5118704251027211
1110	12	0.5054248695298824
1110	13	0.3136962745834566
1110	14	1.1495505045526926
1110	15	0.6357964538090712
1110	16	1.0671874667750885
1110	17	1.3519450553368557
1110	18	1.931706048131744
1110	19	1.1546397704880462
1110	20	0.8501720451395087
1110	21	0.6564757543088575
1110	22	0.9599947035112351
1110	23	2.3522227279093357
1110	24	1.3362105306775192
1110	25	1.6118311048868534
1111	1	0.42525340872851913
1111	2	0.725442129322083
1111	3	0.4615671164203867
1111	4	0.9202648643922231
1111	5	0.60983759374718
1111	6	0.28583274861103547
1111	7	0.18916792719939224
1111	8	0.03779886272053545
1111	9	0.17231897206868751
1111	10	0.3273129634275733
1111	11	0.1884751453438085
1111	12	0.3026566895258682
1111	13	0.14635194855352296
1111	14	-0.546090082839882
1111	15	-0.053653312302323286
1111	16	0.47729410332774336
1111	17	1.0000423151815099
1111	18	1.656338045940565
1111	19	0.8145162045030361
1111	20	0.5342046221716004
1111	21	0.486375082475476
1111	22	0.8066124413729341
1111	23	0.7055023623083727
1111	24	0.5558123759662479
1111	25	0.14876977180560402
1112	1	0.4411460596740824
1112	2	0.7384408763741348
1112	3	0.49290048261755004
1112	4	0.9937305866941557
1112	5	0.8661976589011715
1112	6	0.20582697944156791
1112	7	0.31192686527505487
1112	8	0.2802674575880957
1112	9	0.37144938125295823
1112	10	0.7338557276575415
1112	11	0.3035316673533117
1112	12	0.3133799939221973
1112	13	0.27531317928180954
1112	14	0.5178717514564042
1112	15	0.30168972770508873
1112	16	0.7773615496567885
1112	17	0.9961179909381599
1112	18	1.0242296392050605
1112	19	0.6568611071230421
1112	20	0.5233086084900336
1112	21	0.45977994282655743
1112	22	0.5724521821365585
1112	23	2.162221833481773
1112	24	1.0650095155590762
1112	25	1.1293496883024474
1113	1	0.43809169297217565
1113	2	0.8365235167961771
1113	3	0.6283034765890534
1113	4	0.7366634811169703
1113	5	0.8197679525296735
1113	6	0.4139987540512635
1113	7	0.30446470861787844
1113	8	0.24790244978704834
1113	9	0.3452907748132148
1113	10	0.7186679757491916
1113	11	0.2938378448644414
1113	12	0.3272221838650964
1113	13	0.3123242082415132
1113	14	0.9760849473630273
1113	15	0.5499514217767469
1113	16	0.895683759410133
1113	17	1.2815992944456553
1113	18	1.9149715798682294
1113	19	0.9311848505111229
1113	20	0.6147999862379905
1113	21	0.6225943932751079
1113	22	0.9099596599730688
1113	23	0.6976117708794334
1113	24	0.7225495117839458
1113	25	1.2951071656903537
1114	1	-0.15401022016032684
1114	2	-2.9282007660638847
1114	3	-0.8627069815202191
1114	4	0.3016646100755338
1114	5	0.5523187590147884
1114	6	0.35379075767465196
1114	7	0.2450769659587877
1114	8	0.2907567283724859
1114	9	0.40733006257101323
1114	10	0.5209067642192053
1114	11	0.3557409540900096
1114	12	0.41439947591896953
1114	13	0.2877570482724394
1114	14	0.9383856041020096
1114	15	0.4603580076586624
1114	16	0.7578706660368866
1114	17	1.1203670948055802
1114	18	1.2024174099576186
1114	19	0.637424306306194
1114	20	0.5680641169801035
1114	21	0.4692397783471307
1114	22	0.6759907039883828
1114	23	2.241516617428168
1114	24	1.161914303419067
1114	25	1.0629354983291108
1115	1	0.05312806137054338
1115	2	0.4013672471400227
1115	3	0.11866414963643024
1115	4	0.3361375159159721
1115	5	0.3481050289105312
1115	6	0.01870431129101746
1115	7	-0.17110550291071291
1115	8	-0.2240430068956769
1115	9	-0.2445872822667141
1115	10	-0.21210284456596895
1115	11	-0.13791136476478272
1115	12	-0.21579572295673444
1115	13	-0.3019423503614149
1115	14	0.6044991119622551
1115	15	-0.0946209118432435
1115	16	0.35259425004839073
1115	17	0.8138009281879377
1115	18	1.2797687025680062
1115	19	0.45511738079062525
1115	20	-0.07563591897113753
1115	21	0.17327170958221672
1115	22	0.29998537666870817
1115	23	1.9438067929355753
1115	24	0.63787134169079
1115	25	0.29225651019302035
1116	1	0.12096769919635975
1116	2	0.11204490188019278
1116	3	0.1862237394605195
1116	4	0.6985319149559359
1116	5	0.23874321303469515
1116	6	0.13092962367193905
1116	7	-0.038424174104555675
1116	8	-0.09303218375702471
1116	9	0.00462609224345556
1116	10	0.46042936038548987
1116	11	-0.004169220497971082
1116	12	0.18862066360505736
1116	13	-0.04816721912445843
1116	14	0.712507552076687
1116	15	-0.04617533750028713
1116	16	0.4435352318088661
1116	17	0.7042050185722388
1116	18	1.2659683192366629
1116	19	0.4647331963365886
1116	20	0.18878672374016503
1116	21	0.0509512468546518
1116	22	0.4689193856661902
1116	23	1.7985016918987569
1116	24	0.7969042547246303
1116	25	1.0337833519181245
1201	1	1.01371969822792
1201	2	1.4153947750711566
1201	3	1.1427431632074097
1201	4	1.6053725371787877
1201	5	1.4852117702899577
1201	6	1.028440955695487
1201	7	0.9878915264988279
1201	8	0.9764110592292496
1201	9	1.074389897257575
1201	10	1.4732367745643629
1201	11	1.035908566449855
1201	12	1.2170023527071692
1201	13	1.1389638520384935
1201	14	2.1125224631467034
1201	15	1.4929582981588894
1201	16	1.8524471679125938
1201	17	2.126922942951289
1201	18	2.7582518405040304
1201	19	1.8828068679329988
1201	20	1.5810608671394561
1201	21	1.6214402130525016
1201	22	1.7896580603318455
1201	23	3.295170990083534
1201	24	2.13263634799727
1201	25	2.307654507284461
1202	1	1.0316847177508137
1202	2	1.3234943653252849
1202	3	1.0945263119449145
1202	4	1.643636297790266
1202	5	1.4658582637110271
1202	6	1.0836382618080478
1202	7	0.8941429947342456
1202	8	0.8688536960440629
1202	9	1.0700949222701226
1202	10	1.533003538412757
1202	11	1.1212470457165153
1202	12	1.2018513995596436
1202	13	1.1610853687658675
1202	14	1.9509015806970282
1202	15	1.478137466154699
1202	16	1.6794439062662434
1202	17	2.0176185341648747
1202	18	2.7055826812372743
1202	19	1.7805496800705782
1202	20	1.5758296216059833
1202	21	1.4311275376370034
1202	22	1.9046524223078194
1202	23	3.4065995491480123
1202	24	2.190579099798093
1202	25	2.367949217680433
1203	1	0.8658142736785059
1203	2	1.1586273550384298
1203	3	0.8792145527400237
1203	4	1.5793688104947847
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2211	16	-0.3065385042783575
2211	17	0.25741150201127994
2211	18	-5.057335000292891
2211	19	-1.6307682590875103
2211	20	-0.6921601095629129
2211	21	-0.6324907965807895
2211	22	-0.23611935019946628
2211	23	-4.434700179163389
2211	24	-1.9125055182730506
2211	25	-1.1811860505837046
2212	1	0.4451962825790865
2212	2	0.7056504247001776
2212	3	0.47840555552922837
2212	4	1.0679239631179058
2212	5	1.0012108164422955
2212	6	0.480178255170145
2212	7	0.2629600175205624
2212	8	0.313622628371796
2212	9	0.18871776733614354
2212	10	-3.575692113761157
2212	11	-0.7450197254780448
2212	12	-0.032422303283595966
2212	13	0.17313840713217843
2212	14	0.9211195251599094
2212	15	0.5600564303367292
2212	16	0.7639192258583591
2212	17	1.2822717991354438
2212	18	1.7572482606612994
2212	19	0.9873695525770714
2212	20	0.520981091672482
2212	21	0.4123496174368029
2212	22	0.7444095475619861
2212	23	2.145047023883926
2212	24	1.206413453613557
2212	25	1.2546310675951702
2213	1	-1.265275175979884
2213	2	-3.679889241098767
2213	3	-1.589895393138196
2213	4	-2.987022342953683
2213	5	-1.0345280468872105
2213	6	-0.7487755129886935
2213	7	-0.715958632062609
2213	8	-0.49416220368687647
2213	9	-0.3784846332392462
2213	10	-0.5201186870977494
2213	11	-0.6004168271639543
2213	12	-0.3186814229099326
2213	13	-0.7637084106545444
2213	14	-5.822326567715521
2213	15	-2.3512198048809694
2213	16	-1.6097174834578922
2213	17	-0.4410468907353646
2213	18	0.3116398130354554
2213	19	-0.41069326863144084
2213	20	-0.5734929793713164
2213	21	-0.7627551060227162
2213	22	-0.62912386921586
2213	23	-5.10555087929529
2213	24	-2.5679746113063366
2213	25	-1.9323135187088951
2214	1	-0.16622034032700128
2214	2	-0.028715542921894155
2214	3	-0.19048409081897688
2214	4	0.17481999577093887
2214	5	-0.10399148719526607
2214	6	-0.32228041163033083
2214	7	-0.4164802681069659
2214	8	-0.47320360651999493
2214	9	-0.26485443675223763
2214	10	0.1285026728616927
2214	11	-0.33750694984047414
2214	12	-0.2870799715642818
2214	13	-0.3572628088783958
2214	14	0.12603434991718387
2214	15	-0.2679580102214132
2214	16	0.24341539814151503
2214	17	0.4228629000073951
2214	18	0.8323536104676421
2214	19	-0.13022808253497686
2214	20	-0.20097933607671337
2214	21	-0.307427770268653
2214	22	0.16440382047746027
2214	23	1.562715339178098
2214	24	0.5105490661304373
2214	25	0.4533744829619266
2215	1	-2.238952799342538
2215	2	-4.336103443263799
2215	3	-2.3685542128858863
2215	4	-3.639849361104339
2215	5	-4.6691857029850645
2215	6	-2.1789610527269296
2215	7	-1.6072655839644092
2215	8	-1.922600926517429
2215	9	-1.741936633354669
2215	10	-3.197155191745974
2215	11	-2.0882006010266636
2215	12	-1.6769480846106148
2215	13	-1.6533911852145025
2215	14	-0.9027836593022798
2215	15	-1.555835028695661
2215	16	-2.2378913484463254
2215	17	-6.542861637547574
2215	18	-2.830183533645627
2215	19	-1.994744152475164
2215	20	-1.7321882420280161
2215	21	-1.7873709304525178
2215	22	-1.3905735561242665
2215	23	-6.084607111633346
2215	24	-3.4399665026668096
2215	25	-3.3462975497288205
2216	1	-0.8608744362143383
2216	2	-0.3323263510315577
2216	3	-0.676542136737595
2216	4	-3.1290256517756454
2216	5	-1.074942967131033
2216	6	-0.8255755124643116
2216	7	-0.7969112096565425
2216	8	-0.702962683623376
2216	9	-0.7572691229396469
2216	10	-0.37757231983751893
2216	11	-0.9812093549158121
2216	12	-1.7721136702247051
2216	13	-1.0373889703750763
2216	14	-6.586285742306021
2216	15	-2.9918353978355654
2216	16	-1.4698760027731765
2216	17	-0.5618525672527923
2216	18	0.1495269552010754
2216	19	-0.32290952715217003
2216	20	-0.8530947441637551
2216	21	-1.0903688738159119
2216	22	-0.4601102855447152
2216	23	0.5644924506721978
2216	24	-0.18210250435430453
2216	25	-0.0659226090548728
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91702.0
3	3.0
4	809.0
5	4625.0
6	5839.0
7	5284.0
8	4754.0
9	4784.0
10	4433.0
11	4304.0
12	4294.0
13	4306.0
14	4279.0
15	4367.0
16	4551.0
17	4976.0
18	5381.0
19	6379.0
20	7942.0
21	10060.0
22	12412.0
23	14962.0
24	18309.0
25	21454.0
26	24407.0
27	27810.0
28	31500.0
29	37592.0
30	45405.0
31	51115.0
32	66437.0
33	86597.0
34	113291.0
35	149146.0
36	208905.0
37	290907.0
38	737881.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.230160149949704	18.18489486103172	12.168970020914982	25.415974968103587
2	13.7051403473687	19.746076725995298	42.45187173846601	24.096911188169994
3	19.21050496971389	24.45511411760933	29.00222783530242	27.33215307737436
4	10.38445178224648	16.37848345174735	40.10453066833418	33.13253409767199
5	12.042510245563399	38.18117080026221	36.80866616676806	12.967652787406328
6	31.940990464658125	37.120074011831704	18.326463096406822	12.612472427103343
7	28.221344022618382	31.306616093434737	22.10384707695804	18.36819280698884
8	25.057752565798314	37.146346581411194	20.48074089268326	17.31515996010723
9	26.41418233159825	15.182966338041851	19.859055699595483	38.543795630764414
10	13.48578704740795	29.562658429324312	35.70906775764293	21.242486765624815
11	34.610433518079994	22.772076942881313	23.900879975027607	18.716609564011087
12	22.688319992153726	25.15838721359216	32.170428225459645	19.982864568794465
13	28.849039113489187	21.693242439130653	25.638068438468654	23.819650008911506
14	20.20958810900581	21.91970598817106	28.82067551235871	29.05003039046442
15	23.700558018557867	30.037939980704646	23.276597831489145	22.984904169248342
16	21.812218992179524	28.439229222219865	28.12603296529708	21.62251882030353
17	21.59432844308991	28.357077209133003	28.445919277266945	21.602675070510138
18	21.12713778486116	27.651183296632826	30.2246825445475	20.996996373958517
19	22.88468666793054	27.5243271997759	28.150227355685026	21.440758776608536
20	23.030330751464383	27.499827885882212	28.32696589688431	21.142875465769094
21	22.653513063612163	27.116149553071438	27.839393374941352	22.390944008375048
22	22.35957472495963	27.90296809682628	27.59798077358405	22.139476404630035
23	20.921518428623774	27.43414912946274	29.527673233873454	22.116659208040037
24	22.22767731447223	27.8668592770132	28.11696032198564	21.788503086528923
25	22.222552327596574	27.327348994475454	27.944315939137997	22.505782738789975
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	203.0
1	203.0
2	347.5
3	492.0
4	492.0
5	492.0
6	920.5
7	1349.0
8	1349.0
9	1349.0
10	2373.0
11	3397.0
12	3397.0
13	3397.0
14	6340.5
15	9284.0
16	9284.0
17	9284.0
18	17055.5
19	24827.0
20	24827.0
21	24827.0
22	39264.5
23	53702.0
24	53702.0
25	53702.0
26	75657.5
27	97613.0
28	97613.0
29	97613.0
30	125896.0
31	154179.0
32	154179.0
33	154179.0
34	178281.5
35	202384.0
36	202384.0
37	202384.0
38	224151.5
39	245919.0
40	245919.0
41	245919.0
42	260344.0
43	274769.0
44	274769.0
45	274769.0
46	288268.0
47	301767.0
48	301767.0
49	301767.0
50	284263.5
51	266760.0
52	266760.0
53	266760.0
54	232478.0
55	198196.0
56	198196.0
57	198196.0
58	165823.5
59	133451.0
60	133451.0
61	133451.0
62	106739.5
63	80028.0
64	80028.0
65	80028.0
66	61098.5
67	42169.0
68	42169.0
69	42169.0
70	30590.5
71	19012.0
72	19012.0
73	19012.0
74	13210.0
75	7408.0
76	7408.0
77	7408.0
78	5134.0
79	2860.0
80	2860.0
81	2860.0
82	1947.0
83	1034.0
84	1034.0
85	1034.0
86	662.0
87	290.0
88	290.0
89	290.0
90	185.5
91	81.0
92	81.0
93	81.0
94	50.0
95	19.0
96	19.0
97	19.0
98	14.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12375059046710309
2	0.04874594687351794
3	0.0357344562186911
4	0.031868723487909215
5	0.036394459367848984
6	0.02168581775804473
7	0.021167243855134965
8	0.02437297343675897
9	0.030548717189593445
10	0.03663017477826251
11	0.02168581775804473
12	0.02121438693721767
13	0.016405792564781668
14	0.022204391660954496
15	0.022817251728029675
16	0.022958680974277793
17	0.027578703018382972
18	0.020365811459728964
19	0.034697308412871566
20	0.024325830354676264
21	0.029087281645029564
22	0.037054462517006864
23	0.02899299548086415
24	0.0404959075090444
25	0.03144443574916486
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2121202.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.45362053333952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.41956596525648	65.30635082430283
2	10.349471391188995	15.825091169252337
3	2.4271130885386936	5.566847491879169
4	0.8137031845219976	2.48842217984859
5	0.3552887210673416	1.358155453012902
6	0.1779109162777326	0.8161160209101901
7	0.1063022925715588	0.5689036596662994
8	0.0652446985220447	0.39905547380932344
9	0.05261191153032847	0.3620134007706026
>10	0.20943319833490592	2.761333898810517
>50	0.012506575742945473	0.6610509009443487
>100	0.008293934284273795	1.2815420501599428
>500	0.0011836175875601439	0.5136126890933712
>1k	0.0012459132500620865	1.4769698721680582
>5k	1.2459132500620865E-4	0.6145349153714635
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	7178	0.3383930431896632	No Hit
CCCATGTACTCTGCGTTGATACCAC	5725	0.26989414492349145	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3015	0.14213639247935841	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2384	0.11238910768517096	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.714308208270594E-5	0.0
5	0.0	0.0	0.0	4.714308208270594E-5	0.0
6	0.0	0.0	0.0	4.714308208270594E-5	0.0
7	0.0	0.0	0.0	4.714308208270594E-5	0.0
8	0.0	0.0	0.0	9.428616416541188E-5	0.0
9	0.0	0.0	0.0	1.414292462481178E-4	1.414292462481178E-4
10	0.0	0.0	0.0	1.414292462481178E-4	1.414292462481178E-4
11	0.0	0.0	0.0	1.8857232833082376E-4	1.414292462481178E-4
12	0.0	0.0	0.0	2.357154104135297E-4	1.414292462481178E-4
13	0.0	0.0	0.0	2.357154104135297E-4	1.414292462481178E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACGA	25	0.006038282	18.997005	13
CGGATTC	25	0.006038282	18.997005	14
CGACGAC	35	0.0021728368	16.283148	14
ACACCGA	50	8.722789E-5	15.198679	6
TAGGACC	600	0.0	13.143314	4
GGCGAGG	485	0.0	11.752665	19
GTAGGAC	1140	0.0	11.750919	3
TACACCG	65	8.025037E-4	11.692395	5
CCAACGA	100	1.930308E-6	11.4000845	19
TGGCGAG	1025	0.0	11.214718	18
GACGTGA	450	0.0	11.187125	7
GGACGTG	770	0.0	11.102931	6
TTAGGAC	665	0.0	11.00086	3
GTCCTAC	1305	0.0	10.71538	1
TATGGCG	1030	0.0	10.6981	16
TGTAGGA	1300	0.0	10.67082	2
ATGGCGA	1095	0.0	10.670306	17
AGGACCT	940	0.0	10.611781	5
GTACTAG	135	5.579932E-8	10.569592	1
GGACCTG	845	0.0	10.567129	6
>>END_MODULE