FastQCFastQC Report
Thu 26 May 2016
SRR1513089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513089_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121202
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC82390.38841185327941424No Hit
CCCATGTACTCTGCGTTGATACCAC78010.367763183327189No Hit
GTATCAACGCAGAGTACTTTTTTTT39670.18701660662209443No Hit
GAGTACATGGGAAGCAGTGGTATCA31910.15043357492591466No Hit
TATCAACGCAGAGTACTTTTTTTTT26460.12474059519083991No Hit
GGTATCAACGCAGAGTACTTTTTTT24090.11356768473723859No Hit
CATGTACTCTGCGTTGATACCACTG22890.1079105148873139No Hit
GTATCAACGCAGAGTACATGGGAAG21380.1007919094928253No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG400.005170579514.2950231
TCGAAGT550.003085743912.0821714
TAGGACC6500.012.010534
GCACCGT657.8813976E-411.7175916
GTAGGAC11750.011.5056763
TCGAACT1850.011.2800819
TGTAGGA14150.011.2442422
TAGGACT3150.011.1828584
GGACCTG9500.011.1240026
TGGCGAG10150.010.84622318
AGGACCT10550.010.7388115
CTGTAGG13950.010.6572221
GGACCGT909.311708E-510.5783816
GGACGTG7500.010.5360676
TTAGGAC6200.010.4418853
CTCGAAC2001.8189894E-1210.4394918
GTCCTAC11150.010.4274611
AGGACGT7950.010.4187075
TTAGACC1658.949428E-1010.3860464
GGCGAGA6950.010.37265819
Produced by FastQC (version 0.11.5)