FastQCFastQC Report
Thu 26 May 2016
SRR1513088_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513088_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089954
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26830.24615717727537126No Hit
GTACATGGGAAGCAGTGGTATCAAC16230.14890536664850076No Hit
GGTATCAACGCAGAGTACTTTTTTT15770.1446850050552592No Hit
TATCAACGCAGAGTACTTTTTTTTT14500.13303313717826623No Hit
CCCATGTACTCTGCGTTGATACCAC11350.10413283496367737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC1257.2759576E-1213.6812243
GTCTTAT707.1826034E-613.5851141
GTATTAG1207.2759576E-1012.679441
GTCTTAG1700.012.3065141
TAGGACT1552.5465852E-1111.6472714
CGGTTTC752.0754288E-411.39840313
TAGCACC1104.9507616E-711.2293074
GCCGGTT600.00588188411.08127211
TACACTA951.35770315E-511.0009835
TCCCGTT700.001493096910.85512411
TAGCCCT2650.010.7566664
TAGGGCC803.762419E-410.6894374
TCTTATA1605.4205884E-1010.6879642
AGACTGT2450.010.4688826
GTCTAGC1002.370054E-510.4605371
TAGGACG2550.010.4332634
GTAGCCC2100.010.4056913
GTAACAC1106.032251E-610.3645633
ATAGGAC1201.5199057E-610.2925873
TCCAACG1201.5216974E-610.29164218
Produced by FastQC (version 0.11.5)