##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513088_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1089954
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.234277776860306	31.0	30.0	34.0	25.0	34.0
2	29.012241801030136	31.0	30.0	34.0	16.0	34.0
3	29.33056257420038	31.0	30.0	34.0	23.0	34.0
4	32.21935145886891	35.0	33.0	37.0	19.0	37.0
5	32.42673635768115	35.0	33.0	37.0	22.0	37.0
6	32.88117663681219	37.0	33.0	37.0	26.0	37.0
7	32.864497951289685	37.0	35.0	37.0	26.0	37.0
8	32.84332825055002	37.0	35.0	37.0	26.0	37.0
9	34.19979191782406	39.0	35.0	39.0	25.0	39.0
10	33.71200894716658	39.0	34.0	39.0	19.0	39.0
11	34.05587116520514	39.0	34.0	39.0	25.0	39.0
12	33.891352295601465	39.0	34.0	39.0	23.0	39.0
13	33.9741273484936	39.0	35.0	39.0	25.0	39.0
14	34.289215875165375	40.0	33.0	41.0	17.0	41.0
15	34.8217420184705	40.0	34.0	41.0	22.0	41.0
16	34.41737174229371	39.0	34.0	41.0	17.0	41.0
17	33.965529737952245	39.0	33.0	41.0	11.0	41.0
18	33.380203201236014	39.0	32.0	41.0	10.0	41.0
19	34.27842275912561	39.0	34.0	41.0	18.0	41.0
20	34.52557906113469	39.0	34.0	41.0	18.0	41.0
21	34.55497663204135	40.0	34.0	41.0	18.0	41.0
22	34.14673922018727	40.0	34.0	41.0	10.0	41.0
23	32.46712980547803	39.0	31.0	41.0	2.0	41.0
24	33.56157507564539	39.0	33.0	41.0	2.0	41.0
25	33.31559955741251	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.4544575161175963
1101	2	0.8240574715919742
1101	3	0.6075074760770818
1101	4	0.5231521853029122
1101	5	0.9043295534435032
1101	6	0.6157728472976842
1101	7	0.5160599262939911
1101	8	0.6330498468155881
1101	9	0.6589787275192194
1101	10	0.949606941360166
1101	11	0.5363590160192047
1101	12	0.606426224301444
1101	13	0.6537182939219761
1101	14	-0.5437532244187651
1101	15	0.3041151192733196
1101	16	0.7784242003384776
1101	17	1.513835719669153
1101	18	-4.650621487591238
1101	19	-0.9386995629164403
1101	20	-0.011449781111736002
1101	21	0.5171339390405478
1101	22	1.023427992686564
1101	23	2.839385721227295
1101	24	1.7405071463024555
1101	25	1.9970303920136203
1102	1	0.6659993473095547
1102	2	1.0803286959564993
1102	3	0.7861209108243052
1102	4	1.338377456683979
1102	5	0.9768410771597829
1102	6	0.8775815308249264
1102	7	0.7583352485561079
1102	8	0.7269399341042941
1102	9	0.8828953796457952
1102	10	1.1078749924657103
1102	11	0.9060214599472403
1102	12	0.9378310044904694
1102	13	0.7783018638790793
1102	14	1.748139184098136
1102	15	1.2573430749217849
1102	16	1.4067176234388086
1102	17	1.1886744728443546
1102	18	1.6069288942852538
1102	19	1.3157131230068657
1102	20	1.202407721556419
1102	21	1.2357852261812141
1102	22	1.6101704678656148
1102	23	3.254190978479464
1102	24	2.1045680435353162
1102	25	2.146343970087244
1103	1	0.8507350375248102
1103	2	1.0768948404285155
1103	3	0.9043000442952582
1103	4	1.382566694229773
1103	5	1.252569709527613
1103	6	0.8073601598644515
1103	7	0.8508194924293591
1103	8	0.8628094733380323
1103	9	0.9504199033762504
1103	10	1.2443013333776562
1103	11	0.9563016812409728
1103	12	1.0649803821708161
1103	13	0.9793429432652445
1103	14	1.8549101352323518
1103	15	1.288436439971484
1103	16	1.639991782802511
1103	17	2.0164932997286797
1103	18	1.98941008935671
1103	19	1.6059215411568317
1103	20	1.5354045466099961
1103	21	1.3903683034556522
1103	22	1.671103474767527
1103	23	3.1363726260166445
1103	24	2.13831081366412
1103	25	2.3574357978641416
1104	1	0.70882181115158
1104	2	1.1558247264146893
1104	3	0.9667396067078897
1104	4	1.475455258219867
1104	5	1.3394125910378776
1104	6	0.8370653142127509
1104	7	0.8125780498787307
1104	8	0.808716389834764
1104	9	0.8983403373726304
1104	10	1.294500761384782
1104	11	0.743787092857815
1104	12	0.9219539499442746
1104	13	0.8076989126392533
1104	14	1.8444552341768556
1104	15	1.2046228121772486
1104	16	1.62914956818976
1104	17	2.0244832449661345
1104	18	2.590430955553984
1104	19	1.7073463933492619
1104	20	1.0792056059510458
1104	21	1.212098902509176
1104	22	1.5999737247258423
1104	23	3.20091548059451
1104	24	2.160977634230214
1104	25	2.242429321263266
1105	1	0.5274301454907686
1105	2	0.8666696819558588
1105	3	0.5801149299534032
1105	4	0.6660287423950138
1105	5	0.8969065833348466
1105	6	0.5606008794489057
1105	7	0.3116120624391314
1105	8	0.3296286828570558
1105	9	0.4723819395730757
1105	10	0.933886421171394
1105	11	0.5326623264907084
1105	12	0.6023207398740098
1105	13	0.573853141440388
1105	14	1.125307029538213
1105	15	0.5456833134958359
1105	16	1.0213097443425667
1105	17	1.4599164396742381
1105	18	1.4591952890007036
1105	19	1.0717745734084403
1105	20	0.8926050697384085
1105	21	0.7796909566132513
1105	22	1.2038534024130385
1105	23	2.6279697126875305
1105	24	1.7115934009301697
1105	25	1.644979074554243
1106	1	-0.5100246375948281
1106	2	-0.026815459945378706
1106	3	-0.20017342953021355
1106	4	-2.7036809782363598
1106	5	-0.39307031284139526
1106	6	-0.42708251484982895
1106	7	-0.45046999127273324
1106	8	-0.4336571025316829
1106	9	-0.3556202715548622
1106	10	-0.19876893918377192
1106	11	-0.24842984899981957
1106	12	-0.10838519097680432
1106	13	-0.19461814468696303
1106	14	0.36400089916426026
1106	15	-0.09765856151458507
1106	16	-1.0464419109243295
1106	17	-5.3977273470345075
1106	18	-6.750392037150085
1106	19	-2.8780247804959167
1106	20	-1.5640667480159678
1106	21	-1.0382673579740356
1106	22	-1.2763457005297525
1106	23	-4.6753735678236055
1106	24	-2.1744342555567613
1106	25	-0.8420837984196439
1107	1	0.6651803009022252
1107	2	1.0035472519480457
1107	3	0.7433454350834054
1107	4	0.9481875073159358
1107	5	1.0011377671424029
1107	6	0.557102695909407
1107	7	0.4326283963256756
1107	8	0.4731475974292323
1107	9	0.4232726325655918
1107	10	0.9314304924382455
1107	11	0.5245146314279481
1107	12	0.5825022148285299
1107	13	0.5348726691036205
1107	14	1.4586995332880548
1107	15	0.8118931560312319
1107	16	-5.73051313222367
1107	17	-0.887118766010687
1107	18	0.6370615610078261
1107	19	0.7068663374567805
1107	20	0.6454659237314857
1107	21	0.6596363202190219
1107	22	0.6747529638390546
1107	23	2.6908742814991626
1107	24	1.6884834323546514
1107	25	1.4081883353660345
1108	1	0.15329206862154976
1108	2	0.4375163692781143
1108	3	0.24156083554754204
1108	4	0.5560011139426138
1108	5	0.5786551722367008
1108	6	0.07791077657610401
1108	7	0.0590231833354693
1108	8	0.029775390152543935
1108	9	0.021502939684673095
1108	10	0.5437843302370382
1108	11	0.07915801945167544
1108	12	0.10259190670167584
1108	13	0.0028873965987727956
1108	14	0.7038149793819457
1108	15	0.3769393596631403
1108	16	0.5916585224570241
1108	17	0.6276283247421475
1108	18	0.5258586149489162
1108	19	0.3287849289248541
1108	20	0.20831007150471947
1108	21	0.3228047052334233
1108	22	0.6438604961088146
1108	23	2.3080930776013204
1108	24	1.1353678015022126
1108	25	1.278383913478308
1109	1	0.475101818595288
1109	2	0.7233329455077957
1109	3	0.5352899447762454
1109	4	0.4395889968216906
1109	5	0.4289976043745156
1109	6	0.39426399915785737
1109	7	0.46984496880435955
1109	8	0.3391549482368532
1109	9	0.36481264057962903
1109	10	0.8429530906447553
1109	11	0.5955704809851028
1109	12	0.45221882185048656
1109	13	0.3778372415270397
1109	14	-0.6985631889978805
1109	15	0.03158143757269016
1109	16	-0.14056695063661095
1109	17	1.1100362503628247
1109	18	1.6898664744423897
1109	19	1.0109503881386672
1109	20	0.7469433393422804
1109	21	0.6102007572328247
1109	22	0.6460502708458122
1109	23	-3.69318538163488
1109	24	-1.2932621771958708
1109	25	-0.11793293914241332
1110	1	0.6483403857630705
1110	2	0.9765605755372881
1110	3	0.7823481565205874
1110	4	1.2607791990245545
1110	5	0.9587727891484121
1110	6	0.7103137463623455
1110	7	0.5591322766915141
1110	8	0.5167083171102931
1110	9	0.6258267749856472
1110	10	1.08168968342423
1110	11	0.6523124215555072
1110	12	0.7937326425686422
1110	13	0.5742796521260018
1110	14	1.4453534471946483
1110	15	0.8287126194881722
1110	16	1.2461071863862685
1110	17	1.659871019055771
1110	18	2.36682803006579
1110	19	1.5204922025629344
1110	20	1.099983427644574
1110	21	1.0229509107218036
1110	22	1.3019051625312414
1110	23	2.706129872989081
1110	24	1.8011142549485797
1110	25	2.1000244328578432
1111	1	0.5080676994685263
1111	2	0.7201547114921496
1111	3	0.5404227059117233
1111	4	1.2465775715861014
1111	5	0.9519170048091965
1111	6	0.46290561642522476
1111	7	0.2449061966980679
1111	8	0.030563981202050172
1111	9	0.2598281198761754
1111	10	0.49627301255898004
1111	11	0.39845291491939605
1111	12	0.48709021348945214
1111	13	0.42030446609512495
1111	14	-0.3078490649694885
1111	15	0.054809743155622925
1111	16	0.6823199424243427
1111	17	1.1142438278146187
1111	18	1.8090796938807827
1111	19	0.93952300807981
1111	20	0.7029532038835384
1111	21	0.6670621107309458
1111	22	0.9225610552946932
1111	23	0.9207383264523088
1111	24	0.9586972005006658
1111	25	0.5565862155297125
1112	1	0.36611646949419097
1112	2	0.603354903446295
1112	3	0.4456242343063934
1112	4	0.8572042174219874
1112	5	0.5468089736327357
1112	6	0.11821823560067202
1112	7	0.24643599885258993
1112	8	0.15248468885870636
1112	9	0.15144842331653763
1112	10	0.610834919446475
1112	11	0.1546647829670036
1112	12	0.23010724472824506
1112	13	0.19180061595340447
1112	14	0.5330903843553543
1112	15	0.15318523736717538
1112	16	0.5149032873191572
1112	17	0.7243862317856014
1112	18	0.9086579175408716
1112	19	0.3963085268017039
1112	20	0.18798460085262292
1112	21	0.39008179608431703
1112	22	0.3071007038121749
1112	23	1.9725478494958537
1112	24	0.8863092413338123
1112	25	0.7895913367644383
1113	1	0.0759974798532177
1113	2	0.48666016980223503
1113	3	0.13595680679971167
1113	4	0.38022337663835515
1113	5	0.5133238634739214
1113	6	0.1191867256103265
1113	7	0.025985049562869733
1113	8	-0.1025398216642941
1113	9	0.07082350440435192
1113	10	0.29999363380287747
1113	11	0.11018432847104265
1113	12	-0.05538933214365471
1113	13	-0.0742730257972184
1113	14	0.5707464233844277
1113	15	0.10644116973521278
1113	16	0.44494088598550263
1113	17	0.8892671736885873
1113	18	1.4383720777058429
1113	19	0.5414258832770926
1113	20	0.2524330309599776
1113	21	0.37040492164378236
1113	22	0.5851482853253955
1113	23	0.2511756774638627
1113	24	0.39330936064291677
1113	25	1.0016694601039688
1114	1	-0.3676836817579492
1114	2	-2.944156946597264
1114	3	-1.0912953943813584
1114	4	0.026912253769523886
1114	5	0.3153115643807567
1114	6	0.16244577211082145
1114	7	-0.07885667665148333
1114	8	-0.11092979555827753
1114	9	0.014430049708074932
1114	10	0.32335919750485687
1114	11	-0.07095887088241426
1114	12	0.0729264905835052
1114	13	-0.11365686952547094
1114	14	0.5973379310795934
1114	15	0.1723738832686692
1114	16	0.4817238184257633
1114	17	0.7366215895204249
1114	18	0.9057007945185447
1114	19	0.5081828647470559
1114	20	0.2219915156093677
1114	21	0.13370745879204549
1114	22	0.2832778386159731
1114	23	1.9328082435089868
1114	24	0.8876036357309545
1114	25	0.8833735415190844
1115	1	0.08100132125349191
1115	2	0.22728309699696325
1115	3	9.84794144564205E-4
1115	4	0.09673185396454898
1115	5	0.2639372945362979
1115	6	-0.18523637241934665
1115	7	-0.41743565773197133
1115	8	-0.3983400497623464
1115	9	-0.38065125926602406
1115	10	-0.14404742384675728
1115	11	-0.22673558429815444
1115	12	-0.3705903017989769
1115	13	-0.3886179119629247
1115	14	0.4206159112136305
1115	15	-0.17270599168472245
1115	16	0.2582097028819348
1115	17	0.7205318771849818
1115	18	1.1865451459109764
1115	19	0.33275911162805727
1115	20	-0.0892311304456399
1115	21	-0.08342526460447885
1115	22	0.20503958085553364
1115	23	1.9405423951901355
1115	24	0.7340630386047309
1115	25	0.4177556219963279
1116	1	0.07060250674411961
1116	2	-0.08682068252141661
1116	3	0.11553129182359356
1116	4	0.7319689060690706
1116	5	0.1750371561676971
1116	6	-0.00407198990840385
1116	7	-0.044247872212501704
1116	8	0.0016446100380420603
1116	9	-0.01988842181998507
1116	10	0.37027786815394137
1116	11	0.13596625437816812
1116	12	0.22068787431265235
1116	13	-0.1382759789136898
1116	14	0.724340511678129
1116	15	-0.016255284348119403
1116	16	0.3163929323621204
1116	17	0.6661585396791097
1116	18	1.114412152516465
1116	19	0.39058246002547037
1116	20	-0.06455273773039494
1116	21	0.07986441052076998
1116	22	0.552241375045277
1116	23	1.8584861334740594
1116	24	0.7879777041423566
1116	25	1.0501679297452782
1201	1	0.9406821684852211
1201	2	1.3882337969340135
1201	3	1.068963681964238
1201	4	1.6222151887559093
1201	5	1.3795170475642706
1201	6	0.9992283013864878
1201	7	0.9994684952335646
1201	8	0.8852239085827804
1201	9	0.9947098588493191
1201	10	1.3583346434933503
1201	11	0.8918607335921394
1201	12	1.1020182394742406
1201	13	1.1314199623417238
1201	14	1.7527687601057877
1201	15	1.4268010248389729
1201	16	1.836049804017307
1201	17	2.1581138910070408
1201	18	2.7821518637971536
1201	19	1.884459767395569
1201	20	1.6253516775704
1201	21	1.6074716943015588
1201	22	1.8929467339188406
1201	23	3.475647312846114
1201	24	2.327596961665691
1201	25	2.464739314432272
1202	1	1.046664037356571
1202	2	1.5406844649378577
1202	3	1.170811393915713
1202	4	1.6719276994006904
1202	5	1.5257338431002694
1202	6	1.189594964921504
1202	7	0.9814128280579126
1202	8	0.9963631919779701
1202	9	1.1740731073752713
1202	10	1.6780136001931751
1202	11	1.3870245962990353
1202	12	1.280805339636899
1202	13	1.0724351229294058
1202	14	1.9696386256598544
1202	15	1.4335186053629698
1202	16	1.8451640409942627
1202	17	2.107293605906044
1202	18	2.8178783446248445
1202	19	2.0433997449608725
1202	20	1.7116169721965306
1202	21	1.5125796438283956
1202	22	2.0856662862511826
1202	23	3.61917466140531
1202	24	2.4386509735938873
1202	25	2.6231393651423858
1203	1	1.0717448991207377
1203	2	1.4851633054098272
1203	3	1.1575536845717238
1203	4	1.783553693542295
1203	5	1.538840035766043
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2211	17	0.23500052844059383
2211	18	-4.842332808064558
2211	19	-1.4861202445381565
2211	20	-0.7326651280260421
2211	21	-0.59795130785065
2211	22	-0.2931199623563998
2211	23	-4.829667900802534
2211	24	-1.8723713861546933
2211	25	-0.8930650508595406
2212	1	0.4459472996448497
2212	2	0.6376186597935103
2212	3	0.4833949010444698
2212	4	0.8728251484251928
2212	5	0.7304553303220374
2212	6	0.16627566096543234
2212	7	-0.011204593877856439
2212	8	-0.019445634853525462
2212	9	0.0062120574892503555
2212	10	-3.685909883116178
2212	11	-1.0236715015221876
2212	12	-0.2539036271291053
2212	13	0.12477601703724872
2212	14	0.8722822920516791
2212	15	0.46131904690214043
2212	16	0.7789665770601815
2212	17	1.2972082620246326
2212	18	1.7038606435386043
2212	19	0.9905422248733586
2212	20	0.3072931935696843
2212	21	0.25160017414244606
2212	22	0.29444677230353733
2212	23	1.8006921693855276
2212	24	1.00119846420354
2212	25	0.8616588975922781
2213	1	-1.4110968083209166
2213	2	-3.786652477232728
2213	3	-1.593062824903054
2213	4	-3.5036137681552653
2213	5	-1.4854550430438955
2213	6	-0.9882950152313121
2213	7	-0.8147971829872347
2213	8	-0.6936956113418162
2213	9	-0.5396780958070906
2213	10	-0.7042466648334695
2213	11	-0.7068747367333259
2213	12	-0.5481926330540574
2213	13	-0.915271476614592
2213	14	-5.531270327152683
2213	15	-2.478669667477618
2213	16	-1.7582830232407716
2213	17	-0.5381086758104132
2213	18	0.2669237489651053
2213	19	-0.6248626467527103
2213	20	-0.7246991886366558
2213	21	-0.9308342281899158
2213	22	-0.6917730151506163
2213	23	-5.23197499886426
2213	24	-2.7854092664463224
2213	25	-2.094740989551518
2214	1	0.04956437501406796
2214	2	0.18558098013663127
2214	3	0.012805966554640946
2214	4	0.39883507350831593
2214	5	-0.012624020816573989
2214	6	-0.19286442930461334
2214	7	-0.409168069836106
2214	8	-0.36230806052271447
2214	9	-0.3558094765733344
2214	10	0.11928708322901116
2214	11	-0.5454878643721344
2214	12	-0.38285178623419824
2214	13	-0.3418864674032207
2214	14	0.20292095100984398
2214	15	-0.1737680699701727
2214	16	0.23914209849023393
2214	17	0.43433223311443925
2214	18	1.1097915905817146
2214	19	0.09790118204625742
2214	20	-0.15251583277503755
2214	21	-0.16006493519525122
2214	22	0.2352881921913479
2214	23	1.6759491286649535
2214	24	0.5898275414613678
2214	25	0.29004641826953304
2215	1	-2.13162757003591
2215	2	-3.9843927586541454
2215	3	-2.2223556939705453
2215	4	-3.4792916773177467
2215	5	-4.581129413282056
2215	6	-2.0998410122719484
2215	7	-1.4460566553797776
2215	8	-1.7269166222945316
2215	9	-1.3456059222664507
2215	10	-2.5408076638359063
2215	11	-1.5039520468431036
2215	12	-1.3619839878209632
2215	13	-1.4833762159523047
2215	14	-0.6774307865973839
2215	15	-1.262460624756649
2215	16	-1.9178708796014732
2215	17	-6.750570754184764
2215	18	-2.9354546389779337
2215	19	-1.9113872948477209
2215	20	-1.5610370815405972
2215	21	-1.4924945350505538
2215	22	-1.0858261477397448
2215	23	-6.1921858213051415
2215	24	-3.4089343940912187
2215	25	-2.916766433904499
2216	1	-1.3038456855425906
2216	2	-0.7935919860379066
2216	3	-1.0579901722930884
2216	4	-3.4418135144315656
2216	5	-1.5531825218811477
2216	6	-1.534649902342096
2216	7	-1.5174156829095367
2216	8	-1.4535662530131361
2216	9	-1.4066584780739433
2216	10	-1.032359365538852
2216	11	-1.6597460687572791
2216	12	-2.193735082099156
2216	13	-1.6531626021441426
2216	14	-7.126304946681767
2216	15	-3.8032080100708505
2216	16	-2.269061504108322
2216	17	-1.469707402606538
2216	18	-0.6672233598329456
2216	19	-1.1937539811712483
2216	20	-1.6689274202385036
2216	21	-1.895031100968879
2216	22	-1.34298221492746
2216	23	-0.442003326249349
2216	24	-1.0210131212124267
2216	25	-1.0152307068635764
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	51936.0
3	0.0
4	431.0
5	2671.0
6	3291.0
7	2991.0
8	2821.0
9	2623.0
10	2430.0
11	2421.0
12	2316.0
13	2377.0
14	2347.0
15	2438.0
16	2426.0
17	2566.0
18	2921.0
19	3498.0
20	4308.0
21	5437.0
22	6739.0
23	8145.0
24	9550.0
25	11195.0
26	12981.0
27	14749.0
28	16094.0
29	19680.0
30	23101.0
31	26071.0
32	33524.0
33	44095.0
34	56707.0
35	74673.0
36	105154.0
37	148302.0
38	376945.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.011125685710404	18.198285586211206	13.396200071280814	24.39438865679758
2	14.00293544499345	18.93518063817933	42.80159677927161	24.260287137555615
3	19.75616270137365	23.52899606178166	28.773957647862158	27.940883588982533
4	10.933034509721864	16.213658882201457	39.320359836491356	33.53294677158532
5	12.800069751647424	37.429009343049614	35.879237871473414	13.89168303382955
6	32.489974580859474	36.21704458902664	17.81689042239821	13.476090407715674
7	28.69509920065042	31.28906246415498	20.751063765357724	19.264774569836874
8	25.457323508089306	36.91284837250278	19.392865991869396	18.23696212753852
9	25.85422258930415	16.482345653520543	19.746988781058572	37.916442976116734
10	14.697109586803256	29.28151747151712	33.73580444969815	22.285568491981476
11	34.91185182738154	23.1282048458554	22.719030682097642	19.24091264466541
12	23.15909443367234	24.91188337864958	30.75047556822718	21.1785466194509
13	28.727605736161525	21.406089991814923	25.464769294578392	24.401534977445156
14	21.1223675154852	21.39077770130764	28.002569396650607	29.484285386556554
15	23.959736593853123	28.9941306239011	23.14760434752286	23.89852843472292
16	22.682052811489136	28.41900479478768	26.559269540480397	22.33967285324279
17	22.025159864619813	28.007510516926192	27.513692193614226	22.45363742483977
18	21.52395302443295	27.49784814610216	29.132706527504272	21.84549230196062
19	23.173591309191995	27.186307774058815	27.79193675541088	21.848164161338314
20	23.14966569455329	26.93833840190434	28.074040902175053	21.83795500136732
21	23.44642904667206	26.442023113483295	27.68524696076875	22.4263008790759
22	22.83902858962675	26.902021033580493	27.763078072414327	22.49587230437843
23	21.687571809809082	26.390678398508193	29.262428373730366	22.65932141795236
24	22.59821359736555	27.031821903073155	27.805525880076804	22.56443861948449
25	22.90866141298712	26.56185615734975	27.837312749334874	22.692169680328252
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	100.0
1	100.0
2	191.5
3	283.0
4	283.0
5	283.0
6	592.0
7	901.0
8	901.0
9	901.0
10	1606.0
11	2311.0
12	2311.0
13	2311.0
14	3836.5
15	5362.0
16	5362.0
17	5362.0
18	9097.0
19	12832.0
20	12832.0
21	12832.0
22	19767.0
23	26702.0
24	26702.0
25	26702.0
26	36897.5
27	47093.0
28	47093.0
29	47093.0
30	59800.5
31	72508.0
32	72508.0
33	72508.0
34	83407.0
35	94306.0
36	94306.0
37	94306.0
38	104795.5
39	115285.0
40	115285.0
41	115285.0
42	123786.5
43	132288.0
44	132288.0
45	132288.0
46	139421.0
47	146554.0
48	146554.0
49	146554.0
50	142740.5
51	138927.0
52	138927.0
53	138927.0
54	125473.0
55	112019.0
56	112019.0
57	112019.0
58	96931.0
59	81843.0
60	81843.0
61	81843.0
62	66674.0
63	51505.0
64	51505.0
65	51505.0
66	39836.0
67	28167.0
68	28167.0
69	28167.0
70	20610.5
71	13054.0
72	13054.0
73	13054.0
74	9115.0
75	5176.0
76	5176.0
77	5176.0
78	3517.5
79	1859.0
80	1859.0
81	1859.0
82	1234.5
83	610.0
84	610.0
85	610.0
86	402.5
87	195.0
88	195.0
89	195.0
90	124.0
91	53.0
92	53.0
93	53.0
94	32.5
95	12.0
96	12.0
97	12.0
98	10.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11945458248696735
2	0.04688271248144417
3	0.03440512168403437
4	0.03192795292278389
5	0.034313374692876944
6	0.020551326019263197
7	0.017707169293383025
8	0.0223862658424117
9	0.025597410532921572
10	0.0322949408874136
11	0.01816590424917015
12	0.018808133187272126
13	0.015230000532132549
14	0.0187163861961147
15	0.02110180796620775
16	0.021010060975050322
17	0.025872651506393847
18	0.017982410266855296
19	0.031469217966996775
20	0.021285301948522597
21	0.026331386462180972
22	0.03330415779014527
23	0.02477168761250475
24	0.035781326551395744
25	0.027982832303014623
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1089954.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.11822889450023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.63517241660071	71.89928204193309
2	8.228998877569218	13.350436290464906
3	1.7661467807795501	4.298000964736808
4	0.5768738649244755	1.8717994487279443
5	0.2540080178704231	1.0302340267325647
6	0.1363506706581782	0.6636314947421231
7	0.08423355799615212	0.47830139266910193
8	0.06560523636019441	0.42574244637952324
9	0.04557831036879497	0.3327508631808435
>10	0.18698610172040617	2.616763812858583
>50	0.01162550215985868	0.636227742718185
>100	0.007282739121225683	1.3114911879508453
>500	5.689619354076468E-4	0.30368795406695226
>1k	5.689619354076468E-4	0.7816503328385871
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2683	0.24615717727537126	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1623	0.14890536664850076	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1577	0.1446850050552592	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1450	0.13303313717826623	No Hit
CCCATGTACTCTGCGTTGATACCAC	1135	0.10413283496367737	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.8349398231484997E-4	0.0
11	0.0	0.0	0.0	1.8349398231484997E-4	0.0
12	0.0	0.0	0.0	1.8349398231484997E-4	0.0
13	0.0	0.0	0.0	1.8349398231484997E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	125	7.2759576E-12	13.681224	3
GTCTTAT	70	7.1826034E-6	13.585114	1
GTATTAG	120	7.2759576E-10	12.67944	1
GTCTTAG	170	0.0	12.306514	1
TAGGACT	155	2.5465852E-11	11.647271	4
CGGTTTC	75	2.0754288E-4	11.398403	13
TAGCACC	110	4.9507616E-7	11.229307	4
GCCGGTT	60	0.005881884	11.081272	11
TACACTA	95	1.35770315E-5	11.000983	5
TCCCGTT	70	0.0014930969	10.855124	11
TAGCCCT	265	0.0	10.756666	4
TAGGGCC	80	3.762419E-4	10.689437	4
TCTTATA	160	5.4205884E-10	10.687964	2
AGACTGT	245	0.0	10.468882	6
GTCTAGC	100	2.370054E-5	10.460537	1
TAGGACG	255	0.0	10.433263	4
GTAGCCC	210	0.0	10.405691	3
GTAACAC	110	6.032251E-6	10.364563	3
ATAGGAC	120	1.5199057E-6	10.292587	3
TCCAACG	120	1.5216974E-6	10.291642	18
>>END_MODULE