FastQCFastQC Report
Thu 26 May 2016
SRR1513088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513088_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089954
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39740.3646025428596069No Hit
GGTATCAACGCAGAGTACTTTTTTT26150.23991838187666634No Hit
TATCAACGCAGAGTACTTTTTTTTT25090.23019320081397932No Hit
GTACATGGGAAGCAGTGGTATCAAC19130.17551199408415402No Hit
CCCATGTACTCTGCGTTGATACCAC15440.1416573543470642No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13380.12275747416863464No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG551.1055012E-515.5780785
AGCACCG400.00520485414.2799045
GCCCTAG707.034976E-613.6124451
CCAACGA500.00151228913.28448419
CCGTGCC604.1355548E-412.6501489
ATTAGAC701.06918684E-412.2399193
TGTACGA550.003006123212.123542
GTAGGAC3300.012.1162823
GGGGTGT1209.6915755E-911.899926
GGTCTAC752.0154264E-411.4344541
GTCCTAA2000.011.4344531
TTATACC752.032751E-411.42392354
CAGCACG752.0980029E-411.3851349
CTAGTAC855.206826E-511.1999243
TCCAACG600.00592095511.0714218
TCTATAC1553.0013325E-1011.055413
TAGGACC2100.010.8799284
AGGCTTA1252.172983E-710.6623286
TGTAGGA4200.010.6596422
AATCCCG1252.2729364E-710.62758719
Produced by FastQC (version 0.11.5)