FastQCFastQC Report
Thu 26 May 2016
SRR1513087_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513087_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934937
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC69370.3585129645047875No Hit
CCCATGTACTCTGCGTTGATACCAC56190.2903970516869542No Hit
GAGTACATGGGAAGCAGTGGTATCA29090.1503408121298006No Hit
GTATCAACGCAGAGTACTTTTTTTT25150.1299783920613436No Hit
GTATCAACGCAGAGTACATGGGAAG19910.10289740699567997No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC551.9600891E-413.81855919
CGTGCGC655.4603814E-513.152163510
GTATAGA2250.013.1041511
GTCCTAC4700.012.5465281
GCGATTA550.003071852712.08936313
TGATACG658.0248865E-411.69232417
GGCGAGA2300.011.56553219
GTATTAG2000.011.4132941
GTAGGAC4750.011.3997223
TAGGACC3200.011.2815564
AGGATCG600.0058764811.0833485
GGACCGT1201.2779492E-711.0819166
GGACCGC1201.2779492E-711.0819166
TCGAGGA700.001491349110.8574382
AGGACCG1408.352799E-910.8571585
TAAGGCT2750.010.7093824
TATGGCG3500.010.58545516
CTAGACA1451.4430043E-810.4830454
GCGCGCG750.002650882410.13203811
CAGTCCG750.002650882410.1320389
Produced by FastQC (version 0.11.5)