##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513087_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1934937
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.25635253240803	31.0	30.0	34.0	25.0	34.0
2	29.000204141013377	31.0	30.0	34.0	16.0	34.0
3	29.32829234233466	31.0	30.0	34.0	23.0	34.0
4	32.22694330616449	35.0	33.0	37.0	19.0	37.0
5	32.42269644954849	35.0	33.0	37.0	22.0	37.0
6	32.892340164046686	37.0	33.0	37.0	26.0	37.0
7	32.87239739588421	37.0	35.0	37.0	26.0	37.0
8	32.85260346977705	37.0	35.0	37.0	26.0	37.0
9	34.22027848968726	39.0	35.0	39.0	25.0	39.0
10	33.71317670807887	39.0	34.0	39.0	19.0	39.0
11	34.08217941979506	39.0	34.0	39.0	25.0	39.0
12	33.92179125211828	39.0	34.0	39.0	23.0	39.0
13	34.00373138763691	39.0	35.0	39.0	25.0	39.0
14	34.31775194747943	39.0	33.0	41.0	17.0	41.0
15	34.85278177015582	40.0	34.0	41.0	22.0	41.0
16	34.434287007794055	39.0	34.0	41.0	17.0	41.0
17	33.96714259947481	39.0	33.0	41.0	11.0	41.0
18	33.368993409087736	39.0	32.0	41.0	10.0	41.0
19	34.26861546396601	39.0	34.0	41.0	18.0	41.0
20	34.52903892994966	39.0	34.0	41.0	18.0	41.0
21	34.56038155247432	40.0	34.0	41.0	18.0	41.0
22	34.14023040543439	39.0	34.0	41.0	10.0	41.0
23	32.442449547452966	39.0	31.0	41.0	2.0	41.0
24	33.5390495917955	39.0	33.0	41.0	2.0	41.0
25	33.28122724409115	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6615493933535177
1101	2	0.9978874735238783
1101	3	0.901966186722376
1101	4	0.9432262300519554
1101	5	1.0956320642051054
1101	6	0.8356666819911425
1101	7	0.7969579394172825
1101	8	0.7645843708051316
1101	9	0.887063311075849
1101	10	1.2121362743096995
1101	11	0.9252843369787982
1101	12	0.9703670730234535
1101	13	0.9252079411495799
1101	14	-0.01783633363614001
1101	15	0.49365484730623876
1101	16	1.213060037342757
1101	17	1.792809513819492
1101	18	-4.234660777823446
1101	19	-0.7088892027133582
1101	20	0.32927500881146443
1101	21	0.6976199712342463
1101	22	1.2662882559081936
1101	23	3.1587522428768864
1101	24	1.9299982537774056
1101	25	2.1978972220081374
1102	1	0.9469104031867168
1102	2	1.1648486205323358
1102	3	0.9982692924354062
1102	4	1.6049063759625142
1102	5	1.4724685395938337
1102	6	1.0120030353459697
1102	7	0.8844957150975006
1102	8	0.8357644427681876
1102	9	0.9633529737075222
1102	10	1.442131304990582
1102	11	1.040755386091547
1102	12	1.1367500103730919
1102	13	0.9568248675160476
1102	14	1.9429687922335361
1102	15	1.394229761287292
1102	16	1.5016797805168025
1102	17	1.2964974476160123
1102	18	1.7177205487490141
1102	19	1.3044131379567503
1102	20	1.317556235446311
1102	21	1.2712712737482619
1102	22	1.6970797850545907
1102	23	3.2896627800031624
1102	24	2.2757645803715647
1102	25	2.510392332735293
1103	1	0.5842398636778512
1103	2	0.886032481854496
1103	3	0.6305621193213362
1103	4	0.9005509988751399
1103	5	0.897791762746202
1103	6	0.5388985634113794
1103	7	0.5510286469549825
1103	8	0.4898479395864186
1103	9	0.6637594709160126
1103	10	0.9269144434700891
1103	11	0.6482665983535867
1103	12	0.7130521944304178
1103	13	0.5452447281239685
1103	14	1.293554109826566
1103	15	0.936032552269559
1103	16	1.214476154438401
1103	17	1.4692669621284082
1103	18	1.7422954541042017
1103	19	1.0435071883475118
1103	20	0.9647892451832831
1103	21	0.8953370252097343
1103	22	1.2380263087735202
1103	23	2.8223238332200395
1103	24	1.6487941972614735
1103	25	1.8129105913754628
1104	1	0.8030042891325984
1104	2	1.2403250850308432
1104	3	0.9719271108034491
1104	4	1.469696782978744
1104	5	1.3731860585092406
1104	6	0.8474983097065589
1104	7	0.8397984304357777
1104	8	0.7869040019410178
1104	9	0.9750338797013427
1104	10	1.404799877882354
1104	11	0.9384443520126098
1104	12	1.0916113891694792
1104	13	0.9931475417745688
1104	14	1.8129560029957688
1104	15	1.1942401780686822
1104	16	1.5720592496372419
1104	17	2.059248941758753
1104	18	2.6636762677914447
1104	19	1.8476103143206615
1104	20	1.2429346812441722
1104	21	1.2386631916107902
1104	22	1.6518215085317252
1104	23	3.118175828054355
1104	24	2.1771742186935725
1104	25	2.2392163240053833
1105	1	0.5156556685178479
1105	2	0.86752314085793
1105	3	0.5793555619818989
1105	4	0.7270946867476198
1105	5	0.8166475551965462
1105	6	0.5019005308426472
1105	7	0.4234542086480104
1105	8	0.369049380457362
1105	9	0.6078207538188636
1105	10	0.9876071169395928
1105	11	0.6183570543043473
1105	12	0.6391811730295842
1105	13	0.6316006209534564
1105	14	1.3430071868775926
1105	15	0.6968020874854304
1105	16	1.0587141046388666
1105	17	1.3728846265637884
1105	18	1.3921437381943988
1105	19	0.9913874675736167
1105	20	0.7324711752161477
1105	21	0.6353538889063302
1105	22	1.1467879742643206
1105	23	2.7510414466054556
1105	24	1.6915063844370337
1105	25	1.6238903492036059
1106	1	-0.3530978015040489
1106	2	0.05536995547882739
1106	3	-0.10271078772243314
1106	4	-2.4137545553014874
1106	5	-0.39679691117901683
1106	6	-0.4792128541788614
1106	7	-0.42114306104353716
1106	8	-0.3262068038286472
1106	9	-0.2921787844734425
1106	10	-0.10078162708850869
1106	11	-0.2722685480799143
1106	12	-0.1919760165094999
1106	13	-0.2252198982599296
1106	14	0.5463504122982954
1106	15	-0.02105388049052692
1106	16	-1.045839833785486
1106	17	-5.834512854128171
1106	18	-6.766425729768283
1106	19	-2.8801881907349838
1106	20	-1.4915825260058782
1106	21	-1.0133588123260822
1106	22	-1.382233240050006
1106	23	-5.2110984069639485
1106	24	-2.1365370484058523
1106	25	-0.6191181843088742
1107	1	0.3966195692965009
1107	2	0.6584761153041256
1107	3	0.43730711411141954
1107	4	0.6767662841863356
1107	5	0.8218137835847372
1107	6	0.2891673995517934
1107	7	0.2480292121843064
1107	8	0.26321487485441253
1107	9	0.29928573999109176
1107	10	0.6832174266749433
1107	11	0.49495476590040255
1107	12	0.48485947997154
1107	13	0.3902329248057441
1107	14	1.0918817523581836
1107	15	0.6051665792438996
1107	16	-5.905565141678089
1107	17	-1.1765766863113498
1107	18	0.5114117296092644
1107	19	0.3702780382934989
1107	20	0.24473205727524316
1107	21	0.3883459330660841
1107	22	0.49219150789146937
1107	23	2.421753306632567
1107	24	1.5040775698828455
1107	25	1.1938062910679434
1108	1	0.3210962159361941
1108	2	0.5857301011436
1108	3	0.24068972432514357
1108	4	0.9336173639060164
1108	5	0.6286654808370784
1108	6	0.21759158195246897
1108	7	0.26940785883979856
1108	8	0.20802269924904948
1108	9	0.2779899873584313
1108	10	0.7598179572634507
1108	11	0.3145527821852383
1108	12	0.4105286878685206
1108	13	0.2558797390771801
1108	14	0.9446835536397344
1108	15	0.7103197056448209
1108	16	0.8479362962828603
1108	17	0.9991241492715233
1108	18	0.7949491213226878
1108	19	0.676237651212908
1108	20	0.6352864113310801
1108	21	0.6236566949689717
1108	22	0.9725497969988979
1108	23	2.5910333603612443
1108	24	1.3198863835536585
1108	25	1.4470000197401163
1109	1	0.1098056387046853
1109	2	0.37515885724940645
1109	3	0.2851883023768522
1109	4	0.20465124863597595
1109	5	0.16071074580351308
1109	6	0.11304444751652198
1109	7	0.1281254547939099
1109	8	-0.12686876523036972
1109	9	0.08588668856683057
1109	10	0.4470357238336362
1109	11	0.17658759318004513
1109	12	0.11797193338716738
1109	13	0.10600962209056775
1109	14	-1.0774118376888921
1109	15	-0.34272218752271755
1109	16	-0.5904722952387758
1109	17	0.5585426108392824
1109	18	1.4037464894284852
1109	19	0.5943118175025788
1109	20	0.46944060619479444
1109	21	0.38335130879315216
1109	22	0.3690079798944623
1109	23	-4.507009326652689
1109	24	-1.5108400999371057
1109	25	-0.2762475228236667
1110	1	0.8502349027713478
1110	2	1.0481886449762037
1110	3	0.9014670130835789
1110	4	1.4074629733048312
1110	5	1.146607304700268
1110	6	0.7876713771998141
1110	7	0.683174100289726
1110	8	0.6757559685971728
1110	9	0.8175181622133394
1110	10	1.1192339169703018
1110	11	0.8406143723271384
1110	12	0.9217670754851923
1110	13	0.7246713938287925
1110	14	1.509172641642401
1110	15	0.9745206410997511
1110	16	1.422965741528941
1110	17	1.8026359947516397
1110	18	2.327978744061973
1110	19	1.6173331348873674
1110	20	1.1458229338447836
1110	21	1.0230221019817094
1110	22	1.2472211880525776
1110	23	2.7661984454758084
1110	24	1.7783004927295138
1110	25	2.0315456207025235
1111	1	0.6131932528435868
1111	2	0.7971919955837343
1111	3	0.6516069838916358
1111	4	1.0472126688245993
1111	5	0.8306748002032904
1111	6	0.38607430021004063
1111	7	0.3109778888823911
1111	8	0.1522764882858354
1111	9	0.2518767821573533
1111	10	0.5658294092848948
1111	11	0.28712368799125443
1111	12	0.5050304675804753
1111	13	0.3275684344611349
1111	14	-0.32900329652240856
1111	15	0.1334385464453547
1111	16	0.6013792855728397
1111	17	1.115203793939905
1111	18	1.6588387827937723
1111	19	0.8386744166828919
1111	20	0.5421228497881003
1111	21	0.4636538096891627
1111	22	0.8182230101640471
1111	23	0.7226743610629711
1111	24	0.575538811977431
1111	25	0.12132176280813667
1112	1	0.07574465360683291
1112	2	0.3900580133928031
1112	3	0.1651900978164349
1112	4	0.6396608243137578
1112	5	0.514723553272546
1112	6	-0.26071370677158967
1112	7	-0.06256070236690192
1112	8	-0.1997336883257077
1112	9	-0.15024802924991576
1112	10	0.21738104671351977
1112	11	-0.12451211356481906
1112	12	-0.1249121410637315
1112	13	-0.20541236605952662
1112	14	0.13902690789731764
1112	15	-0.17119935551601628
1112	16	0.24825234417711073
1112	17	0.5450827987619604
1112	18	0.6963043923550529
1112	19	0.06330906449521478
1112	20	-0.14613632839134993
1112	21	-0.09855664500420858
1112	22	-0.1473798949035512
1112	23	1.5059717015357137
1112	24	0.5459556388862836
1112	25	0.5907253660386189
1113	1	0.44201661084946053
1113	2	0.836845414706822
1113	3	0.5060859408958933
1113	4	0.6099605357594697
1113	5	0.829886522276766
1113	6	0.3996973278425031
1113	7	0.2769632658548673
1113	8	0.20495438738564076
1113	9	0.41655446217004055
1113	10	0.6393691319467436
1113	11	0.3279429783626995
1113	12	0.311065031269834
1113	13	0.2865749739832779
1113	14	0.9303943944114437
1113	15	0.4902752314207959
1113	16	0.9183161821561541
1113	17	1.3080973423949303
1113	18	1.8257052592599052
1113	19	0.9310275191358883
1113	20	0.6611333146210399
1113	21	0.5501821202152755
1113	22	0.7635463560848592
1113	23	0.8466131448867529
1113	24	0.8065859871800072
1113	25	1.1603416757728766
1114	1	-0.3689039718793552
1114	2	-3.076667710122262
1114	3	-0.9777873562016275
1114	4	0.08702798760731412
1114	5	0.4286961289202438
1114	6	0.2284665292360586
1114	7	0.24501478052584247
1114	8	0.24105838761865073
1114	9	0.3594271306156003
1114	10	0.49379099463362053
1114	11	0.2195374282639051
1114	12	0.3164592413609526
1114	13	0.30500278985054763
1114	14	1.0446419781062986
1114	15	0.4230281809069538
1114	16	0.8408713368595571
1114	17	1.1865942695247043
1114	18	1.2851591289889726
1114	19	0.6928306216182989
1114	20	0.5118371701912849
1114	21	0.31902691356920343
1114	22	0.534389668020836
1114	23	2.1610313626867423
1114	24	1.0794738338892529
1114	25	1.0272447476722135
1115	1	0.2203141252310985
1115	2	0.46979471504731407
1115	3	0.26796540470133223
1115	4	0.4501171488111737
1115	5	0.41402083133694845
1115	6	-0.04569413729996796
1115	7	-0.11485122006838111
1115	8	-0.035394602753747506
1115	9	-0.12869047721291338
1115	10	0.11592223155607684
1115	11	0.05288959431634055
1115	12	-0.14565578098545018
1115	13	-0.130167747664224
1115	14	0.693016411544356
1115	15	-0.07193321070192127
1115	16	0.434962893901357
1115	17	0.8669739213380154
1115	18	1.3471850185781449
1115	19	0.5838970381653823
1115	20	0.1692568400840102
1115	21	0.20678048362059798
1115	22	0.5775222577383303
1115	23	2.210321461364927
1115	24	0.8282897431381997
1115	25	0.5564787906961541
1116	1	0.2095408926763298
1116	2	0.07567042063554652
1116	3	0.29474143380007334
1116	4	0.8812939302314788
1116	5	0.4306181897996666
1116	6	0.2487130424079993
1116	7	0.07505568032930654
1116	8	0.15472364433242092
1116	9	0.18949803218021088
1116	10	0.6473831600438515
1116	11	0.31794778300161397
1116	12	0.2862017833906947
1116	13	0.1919098849163916
1116	14	0.8391653709080558
1116	15	0.15539600951196064
1116	16	0.6561579939464366
1116	17	0.9308110404312515
1116	18	1.3975777735639525
1116	19	0.5790554688430944
1116	20	0.3526741258151702
1116	21	0.3611094488037381
1116	22	0.7161361484635833
1116	23	2.076385874406135
1116	24	1.0583943822123487
1116	25	1.3776667789395063
1201	1	1.0301221726395227
1201	2	1.3887468724264913
1201	3	1.1175536346627304
1201	4	1.631402128986096
1201	5	1.469637221979319
1201	6	1.0854138247007725
1201	7	0.9610137117247533
1201	8	1.0201001177149962
1201	9	1.1638523339883733
1201	10	1.469727168025159
1201	11	1.1045755612125063
1201	12	1.26922877549098
1201	13	1.1574104433150012
1201	14	2.0494277139877752
1201	15	1.3717585486487849
1201	16	1.8550220112916023
1201	17	2.2803268650405144
1201	18	2.776736795201245
1201	19	1.9220852499955399
1201	20	1.5829413946518898
1201	21	1.5035531226228045
1201	22	1.7648072474560053
1201	23	3.4317218134927217
1201	24	2.286513931168713
1201	25	2.4237974663520987
1202	1	1.0612019987651493
1202	2	1.365372754284735
1202	3	1.1441715475211112
1202	4	1.7605624192417793
1202	5	1.632809639647924
1202	6	1.2408374194318341
1202	7	0.9553388876119655
1202	8	1.0953866989365437
1202	9	1.1892029578714443
1202	10	1.6661437582717866
1202	11	1.301684534015692
1202	12	1.3175169180551762
1202	13	1.23460352903286
1202	14	2.0921169680457936
1202	15	1.59067545256881
1202	16	1.840863835606406
1202	17	2.238073604896023
1202	18	2.916506512904874
1202	19	2.064915281643856
1202	20	1.7891990941832887
1202	21	1.6987429955499564
1202	22	2.1178066918059812
1202	23	3.723593578962877
1202	24	2.41643321416219
1202	25	2.6317861828296216
1203	1	0.8442905016981364
1203	2	1.1512543927710013
1203	3	0.8881633366987671
1203	4	1.3456132052661403
1203	5	1.2016873470545235
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2211	15	-0.4147776171645319
2211	16	-0.6963320255756926
2211	17	-0.09015837969384677
2211	18	-5.783512406181671
2211	19	-2.0827478542036673
2211	20	-1.2256888660045249
2211	21	-0.8952884342287746
2211	22	-0.5229248744668169
2211	23	-4.9898947034741
2211	24	-2.270792899710184
2211	25	-1.5493058411244434
2212	1	0.30756549234256525
2212	2	0.5711226933936544
2212	3	0.3113195003288176
2212	4	0.827183408112937
2212	5	0.713973239756271
2212	6	0.2745908822564047
2212	7	0.0932542273842003
2212	8	0.04409057251982773
2212	9	-0.24530350568837633
2212	10	-3.610369925718299
2212	11	-1.0980486833779892
2212	12	-0.331209035658496
2212	13	-0.051601283525045005
2212	14	0.7149249460293987
2212	15	0.3641201122502622
2212	16	0.5947090789317144
2212	17	1.1464643958882377
2212	18	1.674078745661319
2212	19	0.8821747732545404
2212	20	0.43971877395311054
2212	21	0.30213627909297713
2212	22	0.5642611236523578
2212	23	2.0086690042330133
2212	24	1.0304881276740971
2212	25	0.8880397537785427
2213	1	-1.6138418659243854
2213	2	-3.891764815697428
2213	3	-1.8181468970039667
2213	4	-3.130836391547806
2213	5	-1.2884872892356292
2213	6	-0.9451882270463692
2213	7	-0.886879665771751
2213	8	-0.8103218639756875
2213	9	-0.8097713458812379
2213	10	-0.8601033426771778
2213	11	-0.9052658588436131
2213	12	-0.8389709839774824
2213	13	-1.2366373869722551
2213	14	-6.275674314757712
2213	15	-2.7784159352927773
2213	16	-2.116281787923711
2213	17	-0.9183482399455869
2213	18	-0.1528354398361813
2213	19	-0.7898749505788416
2213	20	-0.8951314614300756
2213	21	-1.2582352227699687
2213	22	-1.0405955114275613
2213	23	-5.792031661399335
2213	24	-3.213712799376818
2213	25	-2.228535886696932
2214	1	0.08954413263307615
2214	2	0.2528011310225402
2214	3	0.006969828784924914
2214	4	0.4554774174938103
2214	5	0.12111004445622342
2214	6	-0.04875754571769875
2214	7	-0.19250953826431783
2214	8	-0.147814671539237
2214	9	0.01189976387041014
2214	10	0.4197411905620072
2214	11	0.01739609799366093
2214	12	-0.034958929152672624
2214	13	-0.19219659747806617
2214	14	0.40083727096127575
2214	15	0.005569093218262822
2214	16	0.47930067942537846
2214	17	0.7686502246289706
2214	18	1.1998734358953485
2214	19	0.2521768798481787
2214	20	0.23297380612440577
2214	21	0.06472714881296326
2214	22	0.5200844436962058
2214	23	2.0098138829888796
2214	24	0.9459481727018826
2214	25	0.8256270820166733
2215	1	-2.295543051271725
2215	2	-4.101781358607262
2215	3	-2.309400145067997
2215	4	-3.751804107540547
2215	5	-5.00665364801813
2215	6	-2.300286772766164
2215	7	-1.7007347347621966
2215	8	-1.8654786355910495
2215	9	-1.7019348158872631
2215	10	-3.095084238310477
2215	11	-2.025945260146301
2215	12	-1.6878172828934694
2215	13	-1.6359968619097884
2215	14	-0.9114735496179733
2215	15	-1.3939503066662198
2215	16	-2.1689213350484877
2215	17	-6.574737245223719
2215	18	-2.9935435841431115
2215	19	-2.114107739961142
2215	20	-1.788692875675963
2215	21	-1.8055558262926326
2215	22	-1.4834846739316916
2215	23	-6.493527439619793
2215	24	-3.7427525626772926
2215	25	-3.253948192788215
2216	1	-0.99387119013738
2216	2	-0.5016877210629147
2216	3	-0.717306248942986
2216	4	-3.458483226564052
2216	5	-1.4266784678442157
2216	6	-0.9478980359131306
2216	7	-0.895221187681905
2216	8	-0.6668174761701948
2216	9	-0.7619173029239477
2216	10	-0.5213391870938722
2216	11	-0.9772973236004248
2216	12	-1.6977454092218807
2216	13	-1.0725054156406912
2216	14	-7.032163431082246
2216	15	-3.3687280154456225
2216	16	-1.6868588995712201
2216	17	-0.7714504302910754
2216	18	0.020772871577889873
2216	19	-0.3775480577802881
2216	20	-0.8654269822699661
2216	21	-1.1303635069220022
2216	22	-0.6780264670318985
2216	23	0.5509096478999638
2216	24	-0.25713160667322654
2216	25	-0.14236064462266995
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91252.0
3	3.0
4	859.0
5	4819.0
6	5871.0
7	5101.0
8	4935.0
9	4497.0
10	4413.0
11	4290.0
12	4143.0
13	4161.0
14	4102.0
15	4243.0
16	4260.0
17	4673.0
18	5293.0
19	6174.0
20	7708.0
21	9480.0
22	11893.0
23	14322.0
24	17209.0
25	19837.0
26	22759.0
27	25841.0
28	28946.0
29	35104.0
30	41311.0
31	46794.0
32	60599.0
33	79378.0
34	102208.0
35	133588.0
36	189441.0
37	263780.0
38	661650.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.00407438929432	17.62852434673958	12.357894355612697	25.0095069083534
2	14.360325331044502	18.95151574691189	41.6329632942612	25.0551956277824
3	19.752597509017676	23.409970857026753	28.299652011082294	28.537779622873277
4	10.96528346093181	15.621072592323	38.308338369548416	35.10530557719677
5	12.993030926555893	37.339757840207625	35.66971348215855	13.997497751077931
6	33.857635620507075	35.26435576673071	17.371913462984818	13.506095149777392
7	29.658825755186985	30.62173503556892	20.692521377020235	19.026917832223862
8	26.311191477197244	36.14018436986458	19.344826855368442	18.203797297569736
9	26.595522497624565	15.548376690850102	19.097985816658756	38.758114994866574
10	14.689198648784185	28.989772822446643	33.792803235142365	22.528225293626807
11	35.54043045036215	22.640514605082778	22.229148962126242	19.589905982428828
12	23.373993825973503	24.679978248238875	30.58856916005533	21.357458765732293
13	29.172748813206077	20.83143804687629	25.222523859933503	24.77328927998412
14	21.38641253273425	20.885099027251233	27.563558467794046	30.164929972220474
15	24.689223948985585	28.53696436103782	22.652069359094178	24.121742330882416
16	23.142771628356343	27.72238330242971	26.3741342118336	22.76071085738035
17	22.727575844829754	27.618647848502125	27.1779525165642	22.47582379010392
18	22.364029972954867	26.85924951823272	28.54739928375886	22.229321225053553
19	23.78318269539463	26.917602951721243	27.17583203271005	22.123382320174077
20	23.97374601121641	26.589374857520365	27.118765853686273	22.31811327757695
21	23.705167646211926	26.303853234153625	27.002829299612646	22.988149820021807
22	23.47791245634579	26.36820241594222	26.769291218492064	23.384593909219927
23	22.043280626178984	26.05475912673652	28.65675592264057	23.24520432444392
24	23.075145321558363	26.999021280049885	26.773755415144258	23.152077983247498
25	23.35656801533975	26.04645247608851	26.821866691205855	23.775112817365887
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	119.0
1	119.0
2	254.0
3	389.0
4	389.0
5	389.0
6	764.0
7	1139.0
8	1139.0
9	1139.0
10	1962.5
11	2786.0
12	2786.0
13	2786.0
14	5021.5
15	7257.0
16	7257.0
17	7257.0
18	12726.0
19	18195.0
20	18195.0
21	18195.0
22	28555.0
23	38915.0
24	38915.0
25	38915.0
26	54899.5
27	70884.0
28	70884.0
29	70884.0
30	91229.5
31	111575.0
32	111575.0
33	111575.0
34	133132.0
35	154689.0
36	154689.0
37	154689.0
38	174942.0
39	195195.0
40	195195.0
41	195195.0
42	214225.0
43	233255.0
44	233255.0
45	233255.0
46	254063.0
47	274871.0
48	274871.0
49	274871.0
50	268016.5
51	261162.0
52	261162.0
53	261162.0
54	235305.0
55	209448.0
56	209448.0
57	209448.0
58	182086.0
59	154724.0
60	154724.0
61	154724.0
62	127794.5
63	100865.0
64	100865.0
65	100865.0
66	78560.5
67	56256.0
68	56256.0
69	56256.0
70	41394.5
71	26533.0
72	26533.0
73	26533.0
74	18642.5
75	10752.0
76	10752.0
77	10752.0
78	7379.0
79	4006.0
80	4006.0
81	4006.0
82	2659.0
83	1312.0
84	1312.0
85	1312.0
86	878.5
87	445.0
88	445.0
89	445.0
90	287.5
91	130.0
92	130.0
93	130.0
94	77.5
95	25.0
96	25.0
97	25.0
98	17.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11018446595418868
2	0.04687491117281855
3	0.03514326306231159
4	0.03173229929449899
5	0.03498821925468375
6	0.01891534453059712
7	0.01979392610715491
8	0.023566658759432476
9	0.028683104411151367
10	0.03509158179310231
11	0.02093091402975911
12	0.019690563568736347
13	0.016176237262505187
14	0.021654451798689054
15	0.02196453941394474
16	0.02211958322157259
17	0.025530546989385186
18	0.020310738799247725
19	0.031422211679243306
20	0.023256571144176785
21	0.027701160296175018
22	0.03628025098491579
23	0.02780452283459358
24	0.0402080274448212
25	0.031835661832917556
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1934937.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.80501550519453
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.53775034690113	56.917439272241154
2	11.769252187792278	16.43105662906769
3	3.3872571203468227	7.093426074176716
4	1.374587237691872	3.838123337612946
5	0.6444659478791703	2.249347774213769
6	0.36424970401311924	1.5255873741838986
7	0.22687429132914544	1.1085874396772715
8	0.14467932608544087	0.8079474080540238
9	0.10064208381590642	0.6322789999119992
>10	0.41878119549123477	4.865379360681678
>50	0.020710023398201	0.9865029079563391
>100	0.008045279654930236	1.1029841214830713
>500	0.001653211756040022	0.7078368003304313
>1k	9.017518669308949E-4	1.074869117798319
>5k	1.5029197782181583E-4	0.6586333826106923
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	6937	0.3585129645047875	No Hit
CCCATGTACTCTGCGTTGATACCAC	5619	0.2903970516869542	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2909	0.1503408121298006	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2515	0.1299783920613436	No Hit
GTATCAACGCAGAGTACATGGGAAG	1991	0.10289740699567997	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.033625384185635E-4	0.0
10	0.0	0.0	0.0	1.033625384185635E-4	0.0
11	0.0	0.0	0.0	3.100876152556905E-4	0.0
12	0.0	0.0	0.0	3.100876152556905E-4	0.0
13	0.0	0.0	0.0	3.100876152556905E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGAC	55	1.9600891E-4	13.818559	19
CGTGCGC	65	5.4603814E-5	13.1521635	10
GTATAGA	225	0.0	13.104151	1
GTCCTAC	470	0.0	12.546528	1
GCGATTA	55	0.0030718527	12.089363	13
TGATACG	65	8.0248865E-4	11.692324	17
GGCGAGA	230	0.0	11.565532	19
GTATTAG	200	0.0	11.413294	1
GTAGGAC	475	0.0	11.399722	3
TAGGACC	320	0.0	11.281556	4
AGGATCG	60	0.00587648	11.083348	5
GGACCGT	120	1.2779492E-7	11.081916	6
GGACCGC	120	1.2779492E-7	11.081916	6
TCGAGGA	70	0.0014913491	10.857438	2
AGGACCG	140	8.352799E-9	10.857158	5
TAAGGCT	275	0.0	10.709382	4
TATGGCG	350	0.0	10.585455	16
CTAGACA	145	1.4430043E-8	10.483045	4
GCGCGCG	75	0.0026508824	10.132038	11
CAGTCCG	75	0.0026508824	10.132038	9
>>END_MODULE