FastQCFastQC Report
Thu 26 May 2016
SRR1513087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513087_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934937
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC80230.41463882286606746No Hit
CCCATGTACTCTGCGTTGATACCAC72110.3726736322681307No Hit
GTATCAACGCAGAGTACTTTTTTTT40870.21122134725833452No Hit
GAGTACATGGGAAGCAGTGGTATCA32640.16868766269909563No Hit
TATCAACGCAGAGTACTTTTTTTTT27160.14036632717240924No Hit
GGTATCAACGCAGAGTACTTTTTTT24710.1277044162161352No Hit
CATGTACTCTGCGTTGATACCACTG22000.11369879226041985No Hit
GTATCAACGCAGAGTACATGGGAAG21190.10951260945446803No Hit
GCGTTGATACCACTGCTTCCCATGT19470.10062343115047157No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACT602.515253E-514.2819244
TAAGGTA701.068605E-412.241654
CTCGCAC550.00301789912.1179973
GGGCGTC550.003099755812.07477511
GCACCGT1603.6379788E-1211.9012956
ACCGCTC802.9043507E-511.8600728
GGGTAGC1251.7505954E-811.43532851
GAAGCGT752.0321304E-411.4252426
AGCACCG1701.0913936E-1111.2015095
CGTGCAT855.405626E-511.15982410
GTAGGAC3850.011.1287733
TGGCGAG2850.010.98759318
TATGGCG3050.010.89159616
TGCACCG700.001464094510.8814665
GGACCGC1053.37635E-610.8811846
ATGGCGA3200.010.67737817
GTGTTAC1105.8273E-610.3957531
TCTACAC3550.010.1917953
GTCCAAT1502.3303073E-810.1647361
GGCGAGG1502.4992914E-810.11902519
Produced by FastQC (version 0.11.5)