FastQCFastQC Report
Thu 26 May 2016
SRR1513086_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513086_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173276
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC36090.3076002577398668No Hit
CCCATGTACTCTGCGTTGATACCAC27070.23072150116426143No Hit
GAGTACATGGGAAGCAGTGGTATCA16620.1416546490339869No Hit
GTATCAACGCAGAGTACTTTTTTTT16210.1381601600987321No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAC350.002169805316.28567719
TGCGTTT601.4693578E-615.82992412
GCACCGC551.128773E-515.5440946
GGACCGT508.717537E-515.198676
AGGACCG801.2861892E-714.25118355
ACCGTCT400.005282641414.2481458
AAACTAT604.0937163E-412.6655586
AAGGCGT1051.9939762E-812.6655586
GCGTTTA905.415295E-712.66393813
TATACTG2200.012.5237675
GTCCTAC4200.012.2314691
ACACCGT701.09135166E-412.2132176
GTACCGT550.003069684612.0898516
CCCGTTT550.00307262712.088304512
TGCAATA1052.7250826E-711.75937112
TCTAACC658.0123916E-411.6937773
ATTATAC1302.6066118E-911.6937773
TAGGACC2200.011.6610544
CTAGACT752.069748E-411.4019194
GTAGGAC3600.011.3486483
Produced by FastQC (version 0.11.5)