##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513086_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1173276
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.471756006259398	31.0	28.0	34.0	16.0	34.0
2	28.223706101548142	31.0	28.0	34.0	16.0	34.0
3	28.54754294812133	31.0	28.0	34.0	16.0	34.0
4	31.402046065887312	35.0	32.0	37.0	16.0	37.0
5	31.584857271434853	35.0	32.0	37.0	17.0	37.0
6	32.0398474016344	35.0	32.0	37.0	19.0	37.0
7	32.008131079132276	35.0	32.0	37.0	19.0	37.0
8	31.985515769520557	35.0	32.0	37.0	18.0	37.0
9	33.26255459073568	38.0	33.0	39.0	17.0	39.0
10	32.72658777644817	37.0	32.0	39.0	11.0	39.0
11	33.098923015556444	38.0	33.0	39.0	17.0	39.0
12	32.944412056498216	38.0	33.0	39.0	16.0	39.0
13	33.01203553128164	38.0	33.0	39.0	16.0	39.0
14	33.2599252008905	39.0	32.0	41.0	10.0	41.0
15	33.74625833989615	39.0	33.0	41.0	11.0	41.0
16	33.30909180789516	39.0	32.0	41.0	10.0	41.0
17	32.8190357597019	38.0	32.0	41.0	8.0	41.0
18	32.19635703790072	38.0	31.0	41.0	8.0	41.0
19	33.099000576164514	39.0	32.0	41.0	2.0	41.0
20	33.36547325607956	39.0	32.0	41.0	2.0	41.0
21	33.39427807267855	39.0	33.0	41.0	2.0	41.0
22	32.95686010793709	39.0	32.0	41.0	2.0	41.0
23	31.21328996757796	38.0	28.0	40.0	2.0	41.0
24	32.25067077141269	38.0	32.0	41.0	2.0	41.0
25	31.974409260907066	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8222887911505659
1101	2	1.268617002600049
1101	3	0.9008715942227603
1101	4	0.8641172677881528
1101	5	1.0412309728762565
1101	6	0.7653890610951279
1101	7	0.7766851771220615
1101	8	0.9156632592596381
1101	9	0.9195666901786836
1101	10	1.3000713441466658
1101	11	0.981054904345342
1101	12	1.0058903718656182
1101	13	0.9340433986743477
1101	14	-0.14686528013594824
1101	15	0.5691848632292604
1101	16	1.1213906580248931
1101	17	1.9349490012787953
1101	18	-4.10805937870958
1101	19	-0.7824473047642542
1101	20	0.21168485739970322
1101	21	0.651119616150936
1101	22	1.1839253396483613
1101	23	3.2008033773067233
1101	24	2.2587279665674274
1101	25	2.413781645808431
1102	1	1.031194466717924
1102	2	1.3313745087896152
1102	3	1.1728046039195021
1102	4	1.8481215026449789
1102	5	1.6335006525136961
1102	6	1.3800038486078279
1102	7	1.1675255043053596
1102	8	1.0933865941093472
1102	9	1.2959198758255965
1102	10	1.76466473962968
1102	11	1.3758096492858485
1102	12	1.3872381697812628
1102	13	1.1952294881465804
1102	14	2.140406432774185
1102	15	1.5611556429525564
1102	16	1.9840020392908926
1102	17	1.6438855731751403
1102	18	2.104635952296036
1102	19	1.7835460769478217
1102	20	1.6524183421348582
1102	21	1.6442441530738137
1102	22	1.9730784918683923
1102	23	3.621082903751333
1102	24	2.5516334043801834
1102	25	2.8321441286021205
1103	1	0.8301000348685079
1103	2	1.2117590321602698
1103	3	0.9472439569248259
1103	4	1.3157165604861376
1103	5	1.31487411818091
1103	6	0.9271998864273456
1103	7	0.8842501397668272
1103	8	0.7891189866444606
1103	9	1.0415125555511224
1103	10	1.3076477253580165
1103	11	1.1244337437645981
1103	12	1.1501484704175837
1103	13	0.9038376018782159
1103	14	1.8664802802102898
1103	15	1.2962530916725825
1103	16	1.6530414788486993
1103	17	2.071159223285825
1103	18	2.0001558816293112
1103	19	1.4269329149148007
1103	20	1.4729103853018728
1103	21	1.4015439767038274
1103	22	1.87848546961348
1103	23	3.3674740271942945
1103	24	2.350815608049224
1103	25	2.3274454802575057
1104	1	0.9473176413122992
1104	2	1.391750067009685
1104	3	1.1093038218915865
1104	4	1.6914675655907132
1104	5	1.491351196236792
1104	6	1.2029894625286204
1104	7	1.1187157577193716
1104	8	1.0495687731140322
1104	9	1.2674821296369743
1104	10	1.7248304094129097
1104	11	1.289027653716957
1104	12	1.2524111284804818
1104	13	1.1628070960421795
1104	14	2.1030290794708435
1104	15	1.5233693710655984
1104	16	1.7481137734060255
1104	17	2.299314487184418
1104	18	2.7762442960833624
1104	19	2.035234887595415
1104	20	1.6250034699738976
1104	21	1.77524133684922
1104	22	2.0078868199458526
1104	23	3.664585372279614
1104	24	2.6403335053391785
1104	25	2.612524238062921
1105	1	1.0572727939446729
1105	2	1.5324082411073512
1105	3	1.239255718733471
1105	4	1.473885609089912
1105	5	1.5467397587823655
1105	6	1.3078499908838026
1105	7	0.9903562175653846
1105	8	1.0287122250773706
1105	9	1.343716922573286
1105	10	1.9177933163294014
1105	11	1.55116693735134
1105	12	1.2765681768083397
1105	13	1.2300781474790838
1105	14	1.9771767272768486
1105	15	1.4331782424068464
1105	16	1.914467471479604
1105	17	2.3635727860109554
1105	18	2.3388873101199437
1105	19	1.9179312782535902
1105	20	1.5034478441054944
1105	21	1.2720164992948213
1105	22	1.702068355486702
1105	23	3.1709868983740925
1105	24	2.3205736084239206
1105	25	2.226914832038336
1106	1	-0.7946037849930505
1106	2	-0.17654085577278167
1106	3	-0.510936975210619
1106	4	-2.944749964103643
1106	5	-0.8431788014274026
1106	6	-0.8463657506746074
1106	7	-0.9377488443744895
1106	8	-0.8263668562808526
1106	9	-0.7161890238672584
1106	10	-0.6091539860144906
1106	11	-0.7983000806566807
1106	12	-0.7139593790167496
1106	13	-0.6526451262846322
1106	14	-0.005644252956059859
1106	15	-0.5914694409509025
1106	16	-1.6284947073096419
1106	17	-6.078159461616131
1106	18	-7.409171564381918
1106	19	-3.2002953227316304
1106	20	-1.8752684595754303
1106	21	-1.6287239537842026
1106	22	-2.080482439464479
1106	23	-5.695229715626841
1106	24	-2.8618410594863875
1106	25	-1.5364643931901476
1107	1	0.7912990959433515
1107	2	1.1337740686381501
1107	3	0.878977856441832
1107	4	1.2132044710796137
1107	5	1.2754107420185434
1107	6	0.8338549699391464
1107	7	0.883440721528153
1107	8	0.900773119016776
1107	9	0.7696777680409426
1107	10	1.29049799379559
1107	11	0.9087918677961113
1107	12	0.9152485767523473
1107	13	0.9423659100392214
1107	14	1.9395593510215292
1107	15	1.214977273203182
1107	16	-5.726135024295026
1107	17	-0.7876715150877018
1107	18	0.9864621029581571
1107	19	1.0786168701121852
1107	20	0.9128473149651839
1107	21	1.0605498065606085
1107	22	1.1971043391104246
1107	23	3.105827097767669
1107	24	2.0912018671080617
1107	25	1.5752899293578118
1108	1	0.8333120591180645
1108	2	1.0499309805402035
1108	3	0.8694373106266653
1108	4	1.423920970626959
1108	5	1.2301101292811367
1108	6	0.8029765123832675
1108	7	0.7482163592195405
1108	8	0.8522307095697172
1108	9	0.8616969929959026
1108	10	1.3499058099302346
1108	11	0.9462679777432257
1108	12	1.0283454985070364
1108	13	0.7723039828953944
1108	14	1.3240667609858718
1108	15	1.0518520229827288
1108	16	1.6221156750326315
1108	17	1.4825190006508606
1108	18	1.1804916029643167
1108	19	1.2641057030797
1108	20	1.093422154988879
1108	21	1.2649987942585739
1108	22	1.7143368443125766
1108	23	3.3302126193854313
1108	24	2.143294147121118
1108	25	2.4281303559842193
1109	1	0.13742912320241274
1109	2	0.5215155737322199
1109	3	0.2911805126776912
1109	4	0.19472335120975615
1109	5	-0.09308093653969607
1109	6	0.07357931702230047
1109	7	0.09438253945538122
1109	8	-0.19165154858375644
1109	9	0.0671695245797892
1109	10	0.4739459548303273
1109	11	0.24211884504569525
1109	12	0.12610729125622555
1109	13	0.2346915934937286
1109	14	-1.0379472275251516
1109	15	-0.18093568905249668
1109	16	-0.3809680860243603
1109	17	0.6724217656590028
1109	18	1.4632466493643008
1109	19	0.5406730423343546
1109	20	0.41455178894612743
1109	21	0.28497259351929216
1109	22	-0.13456056967218188
1109	23	-4.780291898365743
1109	24	-2.0415273916150944
1109	25	-0.7705173606338604
1110	1	0.46614279870515674
1110	2	0.8371162895029336
1110	3	0.5816965507157299
1110	4	1.0601772959706395
1110	5	0.7733926628467245
1110	6	0.38982486371985914
1110	7	0.40712106680353344
1110	8	0.4324206858965596
1110	9	0.4186661081218688
1110	10	0.8326150595746853
1110	11	0.45309930669655785
1110	12	0.5924860806511489
1110	13	0.4555812645153594
1110	14	1.179408870641204
1110	15	0.7683814945317309
1110	16	1.1954691154932675
1110	17	1.5120461369698077
1110	18	2.069523283229472
1110	19	1.1288728879327081
1110	20	0.8125866059030287
1110	21	0.8779920401889498
1110	22	0.9972244799772838
1110	23	2.349068117421016
1110	24	1.3979499012242371
1110	25	1.8048001214676823
1111	1	0.5880339265585164
1111	2	0.8709362662514053
1111	3	0.7760669697474825
1111	4	1.1998005374671479
1111	5	0.8867236025054552
1111	6	0.5452491131499357
1111	7	0.46834329028107646
1111	8	0.3354294687350645
1111	9	0.47667753768845245
1111	10	0.6303080385670228
1111	11	0.36290388419967456
1111	12	0.4746729255997266
1111	13	0.342177026609086
1111	14	-0.5719485936141879
1111	15	0.11099458807876061
1111	16	0.5548978190318934
1111	17	1.135171623845153
1111	18	1.7991723416914382
1111	19	0.8246887428947645
1111	20	0.7469918388467889
1111	21	0.6071282864754721
1111	22	0.9953539968784497
1111	23	0.8370514759632535
1111	24	0.7274354439715793
1111	25	0.2369630067071462
1112	1	0.3397030986799585
1112	2	0.7787317705191157
1112	3	0.5300394728398672
1112	4	0.9218909451336188
1112	5	0.7875763742060684
1112	6	0.027306131871512207
1112	7	0.08087498446739261
1112	8	-0.06977657649077429
1112	9	0.1970426481118821
1112	10	0.5072072136377841
1112	11	0.11981745371338803
1112	12	0.1725992674303356
1112	13	-0.010967190742597666
1112	14	0.6099482484123016
1112	15	0.3195990094177503
1112	16	0.7309502200866049
1112	17	0.8224872913379215
1112	18	0.7019878419093359
1112	19	0.26299430346367103
1112	20	0.3606655692084999
1112	21	0.2714901548477542
1112	22	0.43796028245187557
1112	23	1.8912609151082158
1112	24	1.062403925864075
1112	25	1.1108660907275585
1113	1	0.18287056317095107
1113	2	0.5615142702133795
1113	3	0.33236066119046725
1113	4	0.37777329807479276
1113	5	0.476811423735235
1113	6	0.16608607212204163
1113	7	0.03602466388177561
1113	8	-0.12271141986679623
1113	9	-0.04241056560604051
1113	10	0.35491921943802396
1113	11	0.13707732023796382
1113	12	-0.015220663036657811
1113	13	-0.009611034974682298
1113	14	0.6562246071886619
1113	15	0.2822271999871191
1113	16	0.4402349734890052
1113	17	0.9647209519539359
1113	18	1.5253250770112743
1113	19	0.643503003825316
1113	20	0.17926608633842278
1113	21	0.2569021990833278
1113	22	0.3720862717249247
1113	23	0.4089619144268575
1113	24	0.2896288075007192
1113	25	0.7225480296153783
1114	1	-0.3030597184553976
1114	2	-3.0773154570860584
1114	3	-1.1195527498090243
1114	4	0.0969550196475879
1114	5	0.4733377669672123
1114	6	0.3025910067141169
1114	7	-0.05280130751592438
1114	8	0.07083516767553988
1114	9	0.09373700598255397
1114	10	0.33579505153596045
1114	11	0.13478622017853326
1114	12	0.20846648360479492
1114	13	0.009930700826352279
1114	14	0.7744863537713442
1114	15	0.2531769570686535
1114	16	0.34060470887467886
1114	17	0.8780540717163845
1114	18	0.8792934081315735
1114	19	0.25161884467229356
1114	20	0.23176751689511832
1114	21	0.1096272162610532
1114	22	0.21241073744790384
1114	23	1.9035550729413764
1114	24	0.7964109718280525
1114	25	0.7172615979208707
1115	1	-0.01940850138108985
1115	2	0.16435650748049468
1115	3	0.007641589635952073
1115	4	0.06585805316560212
1115	5	0.18827322421740078
1115	6	-0.2444081399682041
1115	7	-0.382184854217666
1115	8	-0.4078982726100584
1115	9	-0.5305964675233668
1115	10	-0.12933151027225165
1115	11	-0.26626109713458135
1115	12	-0.41686681619629695
1115	13	-0.5055462826985888
1115	14	0.42986824998475015
1115	15	-0.4080126461415574
1115	16	0.17314184632433438
1115	17	0.629691254376084
1115	18	1.2082406457276136
1115	19	0.46388614891952074
1115	20	-0.00479682378391999
1115	21	-0.009279004769858545
1115	22	0.29799107242334344
1115	23	2.042858744663814
1115	24	0.7813439784949239
1115	25	0.5488898555444344
1116	1	-0.26156858020436147
1116	2	-0.47108719962213286
1116	3	-0.22055278534000067
1116	4	0.3590802889562532
1116	5	-0.22427325926433994
1116	6	-0.3485073850246039
1116	7	-0.3606255519173729
1116	8	-0.4435674758292336
1116	9	-0.465104231916726
1116	10	0.07002010475167708
1116	11	-0.35671347780815665
1116	12	-0.14978138695908427
1116	13	-0.5063408721300462
1116	14	0.20063973777400435
1116	15	-0.5401587234626319
1116	16	0.08557206882610302
1116	17	0.1865204577959929
1116	18	0.7389447523330759
1116	19	0.1088127372201555
1116	20	-0.08160602027836461
1116	21	-0.285214654735654
1116	22	0.04662513137016333
1116	23	1.524188234972332
1116	24	0.4383068402213084
1116	25	0.7118114688788175
1201	1	1.1987752808523204
1201	2	1.5421375135269777
1201	3	1.2755205332843467
1201	4	1.8217947306749025
1201	5	1.7313282404347916
1201	6	1.206926795703886
1201	7	1.1668663607133212
1201	8	1.1653590598280488
1201	9	1.2512091962878387
1201	10	1.7098094474246963
1201	11	1.4293705896885882
1201	12	1.5538425430823892
1201	13	1.5372614915932772
1201	14	2.3071372339896143
1201	15	1.7831731869737126
1201	16	2.0012275357506013
1201	17	2.431535775367742
1201	18	3.0298160967307197
1201	19	2.159894053211289
1201	20	1.8309321022176377
1201	21	1.7777452795710786
1201	22	1.9843265870109263
1201	23	3.8003012232788613
1201	24	2.583620280426203
1201	25	2.5306979405571646
1202	1	1.1545045420971718
1202	2	1.585930530595519
1202	3	1.2520004228775576
1202	4	1.8556213993406132
1202	5	1.7028577041293431
1202	6	1.4446397411545249
1202	7	1.2000936267949882
1202	8	1.2442756404334148
1202	9	1.4716049634067403
1202	10	1.894333872226369
1202	11	1.4536068170096144
1202	12	1.5038816703199416
1202	13	1.4212561388754352
1202	14	2.2840234508653268
1202	15	1.858910854133626
1202	16	2.0008798760436477
1202	17	2.5069614800227527
1202	18	3.0811080095140753
1202	19	2.3235449271253543
1202	20	1.9676852895897667
1202	21	1.8509301311684965
1202	22	2.213235981614311
1202	23	4.014240142572451
1202	24	2.6156863010045512
1202	25	2.7382129155334276
1203	1	1.0518551469992872
1203	2	1.4687921696787782
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2211	16	-0.8790042542084677
2211	17	-0.5531998678940795
2211	18	-5.597681062054821
2211	19	-2.1361372442303512
2211	20	-1.602812648631101
2211	21	-1.4969675494331014
2211	22	-0.9694621619005979
2211	23	-5.681277682772052
2211	24	-3.140768028142368
2211	25	-2.2226333154589
2212	1	0.2064672367676259
2212	2	0.43267851137762037
2212	3	0.24712157595137896
2212	4	0.7937192440207355
2212	5	0.72036651729033
2212	6	0.2800985461345178
2212	7	0.16384171169882933
2212	8	0.136810409655034
2212	9	0.06596781277234243
2212	10	-3.6118167472979437
2212	11	-1.3357293325686932
2212	12	-0.42540777322376755
2212	13	-0.08374824911189194
2212	14	0.7195718005358529
2212	15	0.26956607732599736
2212	16	0.413602956856991
2212	17	1.076176253409237
2212	18	1.5297325614187613
2212	19	0.9011745214968343
2212	20	0.351386668459007
2212	21	0.11056124274237078
2212	22	0.5038534234920746
2212	23	1.6422051576701016
2212	24	1.0552213230932352
2212	25	0.8606145576819024
2213	1	-1.380559790259401
2213	2	-3.6500241913250804
2213	3	-1.7172027483729444
2213	4	-3.206821296519802
2213	5	-1.3736875367637218
2213	6	-1.1599014538654835
2213	7	-0.9064285585714451
2213	8	-0.872919320074697
2213	9	-0.6788970520925233
2213	10	-0.834519450000645
2213	11	-0.6638374406768008
2213	12	-0.5291915570075503
2213	13	-0.9842887896524353
2213	14	-6.047455226491174
2213	15	-2.875839328079408
2213	16	-2.1274781242240905
2213	17	-0.9065264492934659
2213	18	-0.021078249392054715
2213	19	-0.7507173703950585
2213	20	-0.7307754937031561
2213	21	-1.0261955140143826
2213	22	-0.8464168467781974
2213	23	-5.587524572059628
2213	24	-3.046940839068924
2213	25	-2.149655712588366
2214	1	-0.07533186281401072
2214	2	-0.09279261588247323
2214	3	-0.09531244886079193
2214	4	0.15321518345240293
2214	5	-0.060957763605454574
2214	6	-0.28558798667574337
2214	7	-0.3393766325157692
2214	8	-0.5625370041755069
2214	9	-0.3379815785043121
2214	10	0.20158529963383387
2214	11	-0.2556430545391919
2214	12	-0.23097531183897502
2214	13	-0.25367051202826474
2214	14	0.2320071282041738
2214	15	-0.08978973268862234
2214	16	0.31135828047197833
2214	17	0.559944981966396
2214	18	1.172857389514128
2214	19	0.35767418130661355
2214	20	0.02639846654525968
2214	21	-0.056215056856636636
2214	22	0.4718767011886271
2214	23	2.114396996000128
2214	24	1.034961909957552
2214	25	0.8125689866690102
2215	1	-3.0947741466146645
2215	2	-4.996947302311547
2215	3	-3.142888313785434
2215	4	-4.467256740155683
2215	5	-5.937585864364944
2215	6	-3.2077544953285724
2215	7	-2.5795751185667193
2215	8	-2.9031243476035904
2215	9	-2.761021721819599
2215	10	-4.492460908663023
2215	11	-3.2909739928488513
2215	12	-2.9066010363940933
2215	13	-2.8871134165731824
2215	14	-2.0918979324412526
2215	15	-2.8577372718998006
2215	16	-3.4262169613681728
2215	17	-7.702335267396112
2215	18	-4.205531583119008
2215	19	-3.471473418633355
2215	20	-3.1564584021714275
2215	21	-3.1683266784939903
2215	22	-2.9420196040958615
2215	23	-7.051342600902231
2215	24	-4.6955204071678835
2215	25	-4.715402387717891
2216	1	-2.069694786438099
2216	2	-1.6295454863990742
2216	3	-1.7840915137450004
2216	4	-4.012329529688703
2216	5	-2.5014565911651694
2216	6	-2.305482639163891
2216	7	-2.1707438507273515
2216	8	-2.19411365065206
2216	9	-2.177302526976149
2216	10	-1.845854126579539
2216	11	-2.357058450890097
2216	12	-3.3654723879670456
2216	13	-2.646314774506184
2216	14	-7.902147785373963
2216	15	-4.620170600225592
2216	16	-3.2505094589006696
2216	17	-2.295277230923425
2216	18	-1.5682914780448698
2216	19	-2.027441472389082
2216	20	-2.7628438602702374
2216	21	-2.8535935547286186
2216	22	-2.2094023258305135
2216	23	-0.9520469093069011
2216	24	-1.7928298128756381
2216	25	-1.6561283034311352
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	72488.0
3	0.0
4	582.0
5	3679.0
6	4187.0
7	3676.0
8	3582.0
9	3344.0
10	3273.0
11	3026.0
12	3065.0
13	2978.0
14	2994.0
15	3137.0
16	3127.0
17	3441.0
18	3765.0
19	4518.0
20	5443.0
21	6998.0
22	8494.0
23	10287.0
24	12165.0
25	13944.0
26	16008.0
27	18363.0
28	19827.0
29	23839.0
30	27731.0
31	31536.0
32	40005.0
33	51448.0
34	65278.0
35	83799.0
36	114860.0
37	154900.0
38	343489.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.44753277361693	17.157990038424682	12.056458597682573	25.33801859027582
2	14.611035788288865	18.524724361106138	41.63976362845668	25.224476222148322
3	20.441641777173743	22.540629628145435	27.93244836726916	29.085280227411666
4	11.03535415972165	15.160759244370592	38.13392923370475	35.669957362203014
5	13.032490636145289	37.28830611641107	35.63542482868814	14.043778418755505
6	34.34190762138099	35.218432076600834	17.0868562250968	13.352804076921371
7	30.058693400508936	30.32091692987055	20.1101407021896	19.51024896743092
8	26.321389203138306	36.62131528940169	18.7757088941158	18.2815866133442
9	26.366382393538252	15.46141169188692	18.714197656643645	39.45800825793119
10	14.558693752264832	29.080553364738986	33.74569948628312	22.615053396713066
11	36.30277646826144	22.391024324040927	21.81849336380694	19.487705843890687
12	23.461238783551313	24.554368341099387	30.47609061475431	21.508302260594995
13	29.553843452227714	20.638640304291417	24.752319949262034	25.055196294218838
14	21.345957277395144	20.819789106477444	27.456225826059306	30.378027790068106
15	24.800770660346796	28.5152853318784	22.330991798946307	24.352952208828494
16	23.247241280536876	27.823765907819897	26.261457722368476	22.667535089274754
17	23.017900196769165	27.352107680817905	26.93793757268012	22.69205454973281
18	22.528841653716018	26.953487638560564	28.37543039769216	22.142240310031262
19	23.88471113839438	26.670247595661962	27.05298069708751	22.39206056885615
20	23.732590624235403	26.75065622168059	27.203937244832282	22.312815909251725
21	23.875195342972386	26.163894565522273	26.96734777741686	22.993562314088482
22	23.366313165160065	26.518922476932023	26.811119694280027	23.303644663627885
23	22.643756655302035	25.882123251291418	28.327949412457787	23.146170680948764
24	23.039045153632856	27.01288203575046	26.93639814596912	23.01167466464756
25	23.584744794517512	26.046084143719234	26.802501155258174	23.566669906505084
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	88.0
1	88.0
2	159.0
3	230.0
4	230.0
5	230.0
6	483.0
7	736.0
8	736.0
9	736.0
10	1196.0
11	1656.0
12	1656.0
13	1656.0
14	2867.0
15	4078.0
16	4078.0
17	4078.0
18	7222.0
19	10366.0
20	10366.0
21	10366.0
22	16130.0
23	21894.0
24	21894.0
25	21894.0
26	31226.0
27	40558.0
28	40558.0
29	40558.0
30	53261.5
31	65965.0
32	65965.0
33	65965.0
34	78041.0
35	90117.0
36	90117.0
37	90117.0
38	103320.0
39	116523.0
40	116523.0
41	116523.0
42	128750.5
43	140978.0
44	140978.0
45	140978.0
46	153785.5
47	166593.0
48	166593.0
49	166593.0
50	163395.0
51	160197.0
52	160197.0
53	160197.0
54	145534.0
55	130871.0
56	130871.0
57	130871.0
58	113474.0
59	96077.0
60	96077.0
61	96077.0
62	79364.0
63	62651.0
64	62651.0
65	62651.0
66	49172.0
67	35693.0
68	35693.0
69	35693.0
70	26324.0
71	16955.0
72	16955.0
73	16955.0
74	12015.0
75	7075.0
76	7075.0
77	7075.0
78	4869.0
79	2663.0
80	2663.0
81	2663.0
82	1806.0
83	949.0
84	949.0
85	949.0
86	613.0
87	277.0
88	277.0
89	277.0
90	171.5
91	66.0
92	66.0
93	66.0
94	39.0
95	12.0
96	12.0
97	12.0
98	10.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11702276361231288
2	0.04653636484510039
3	0.03622336091422649
4	0.03179132616707407
5	0.035371046539774104
6	0.01994415636218588
7	0.019688462049850163
8	0.024461422546783535
9	0.029234383043716908
10	0.0384393782878027
11	0.020370313549412072
12	0.018921379112843015
13	0.016193973114595372
14	0.02275679379787876
15	0.021819247985981133
16	0.022842025235324
17	0.027955911482038326
18	0.019858924924740638
19	0.03375164922831457
20	0.023268182422550195
21	0.028893457293935956
22	0.037331369601014595
23	0.028893457293935956
24	0.04091108997371463
25	0.03221748335430027
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1173276.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.05453425145811
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.73739248630513	73.63359945404063
2	7.496916579170569	12.303119964517512
3	1.4975167326275551	3.686341140885581
4	0.5162571800236685	1.6944496984325315
5	0.24855582471225396	1.019756621612553
6	0.13232416276379116	0.6514678527478213
7	0.08442415848058285	0.484916950258686
8	0.059112790237098145	0.3880377976967383
9	0.03904608450981866	0.2883517450916619
>10	0.17107368712796459	2.4362966624743794
>50	0.00870179875898029	0.47496599916889887
>100	0.006900994668578737	1.2581191552971254
>500	0.0011501603973308697	0.6807074526748182
>1k	6.273602167259289E-4	0.9998695051011256
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3609	0.3076002577398668	No Hit
CCCATGTACTCTGCGTTGATACCAC	2707	0.23072150116426143	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1662	0.1416546490339869	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1621	0.1381601600987321	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	8.52314374452388E-5	0.0
9	0.0	0.0	0.0	8.52314374452388E-5	0.0
10	0.0	0.0	0.0	8.52314374452388E-5	0.0
11	0.0	0.0	0.0	8.52314374452388E-5	0.0
12	0.0	0.0	0.0	8.52314374452388E-5	0.0
13	0.0	0.0	0.0	8.52314374452388E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGAC	35	0.0021698053	16.285677	19
TGCGTTT	60	1.4693578E-6	15.829924	12
GCACCGC	55	1.128773E-5	15.544094	6
GGACCGT	50	8.717537E-5	15.19867	6
AGGACCG	80	1.2861892E-7	14.2511835	5
ACCGTCT	40	0.0052826414	14.248145	8
AAACTAT	60	4.0937163E-4	12.665558	6
AAGGCGT	105	1.9939762E-8	12.665558	6
GCGTTTA	90	5.415295E-7	12.663938	13
TATACTG	220	0.0	12.523767	5
GTCCTAC	420	0.0	12.231469	1
ACACCGT	70	1.09135166E-4	12.213217	6
GTACCGT	55	0.0030696846	12.089851	6
CCCGTTT	55	0.003072627	12.0883045	12
TGCAATA	105	2.7250826E-7	11.759371	12
TCTAACC	65	8.0123916E-4	11.693777	3
ATTATAC	130	2.6066118E-9	11.693777	3
TAGGACC	220	0.0	11.661054	4
CTAGACT	75	2.069748E-4	11.401919	4
GTAGGAC	360	0.0	11.348648	3
>>END_MODULE