FastQCFastQC Report
Thu 26 May 2016
SRR1513086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513086_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173276
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC42980.36632471813963635No Hit
CCCATGTACTCTGCGTTGATACCAC38400.327288719789717No Hit
GTATCAACGCAGAGTACTTTTTTTT25460.21699923973557797No Hit
GGTATCAACGCAGAGTACTTTTTTT18520.15784862214858225No Hit
TATCAACGCAGAGTACTTTTTTTTT18380.15665538202434892No Hit
GAGTACATGGGAAGCAGTGGTATCA17450.14872885834194172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGC551.9844052E-413.7976278
TGGCGAG2500.013.66605918
GACGTGA1051.4006218E-913.551247
CTAGCAC1207.1304385E-1012.6949073
GGCGAGA1950.012.64674619
GTCTAGA1155.0913513E-912.4274391
GGACGTG1700.012.3215276
TATGGCG2800.012.205482516
TAGGACG1650.012.1178664
CTGGACG550.003016921212.1178664
TAGGACC2300.012.0049664
GGCGAGG951.0589774E-611.98112719
GACCGTG802.9089284E-511.8573367
AGGACGT1700.011.7614575
CTCCTAA907.218203E-611.644971
CCCGTGC907.5806565E-611.5938398
GTTCTAG1655.456968E-1211.5487311
ATGGCGA3050.011.51579317
GTAGGAC3400.011.4814223
CTAGGAC1900.011.0245243
Produced by FastQC (version 0.11.5)