##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513086_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1173276 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.60740439589662 31.0 31.0 34.0 27.0 34.0 2 30.73243976694316 31.0 31.0 34.0 27.0 34.0 3 30.74993692873629 31.0 31.0 34.0 27.0 34.0 4 34.00856320252012 37.0 35.0 37.0 30.0 37.0 5 33.975033155029166 37.0 35.0 37.0 30.0 37.0 6 33.950328822885666 37.0 35.0 37.0 30.0 37.0 7 33.86272454222195 37.0 35.0 37.0 29.0 37.0 8 33.857753844790146 37.0 35.0 37.0 29.0 37.0 9 35.40815289838026 39.0 35.0 39.0 29.0 39.0 10 35.317092482928146 39.0 35.0 39.0 29.0 39.0 11 35.34230479443882 39.0 35.0 39.0 29.0 39.0 12 35.24878715664516 39.0 35.0 39.0 29.0 39.0 13 35.23631864966129 39.0 35.0 39.0 29.0 39.0 14 36.41157579290806 40.0 36.0 41.0 29.0 41.0 15 36.40318475789158 40.0 36.0 41.0 29.0 41.0 16 36.36063381506142 40.0 36.0 41.0 29.0 41.0 17 36.26751420808062 40.0 36.0 41.0 28.0 41.0 18 36.18637132268963 40.0 36.0 41.0 27.0 41.0 19 36.25780719966998 40.0 37.0 41.0 27.0 41.0 20 36.14779557410191 40.0 36.0 41.0 27.0 41.0 21 36.16965829012099 40.0 36.0 41.0 27.0 41.0 22 36.143486272624685 40.0 36.0 41.0 27.0 41.0 23 36.08088378182116 40.0 36.0 41.0 27.0 41.0 24 35.955877389463346 40.0 36.0 41.0 27.0 41.0 25 35.944831395170446 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3893549254150557 1101 2 0.3194933136482341 1101 3 0.42964305318146145 1101 4 0.6200523549166377 1101 5 0.5283142917140466 1101 6 0.4045548835215058 1101 7 0.30592468086864955 1101 8 0.2125139524779769 1101 9 0.3073194415434912 1101 10 0.27302244459463765 1101 11 0.24469893760029748 1101 12 0.28517573879522473 1101 13 0.3868028615133454 1101 14 0.3538004946137008 1101 15 0.284836232530445 1101 16 0.3356902432311131 1101 17 0.3042340304323119 1101 18 0.10342276451458332 1101 19 0.17832422974657192 1101 20 0.4034993137154572 1101 21 0.503049068321566 1101 22 0.4475991217742603 1101 23 0.42915479146486035 1101 24 0.48308812219062247 1101 25 0.49097015586398385 1102 1 0.7264903708018196 1102 2 0.6885181843103148 1102 3 0.6258768201030875 1102 4 0.9615221138840795 1102 5 0.8369848020802024 1102 6 0.740643090481008 1102 7 0.7104950424027834 1102 8 0.7786409240315777 1102 9 0.6351612711711283 1102 10 0.6561298620087541 1102 11 0.5995341542365296 1102 12 0.5683720787866662 1102 13 0.6840184843450814 1102 14 0.7101346245819045 1102 15 0.9062976465465482 1102 16 0.8369413974296904 1102 17 0.8602779114584962 1102 18 0.6871050301982891 1102 19 0.7025955030460835 1102 20 0.8244617961560436 1102 21 0.8497067623019632 1102 22 0.8944047650328883 1102 23 0.7933786668532008 1102 24 0.9142425679369666 1102 25 1.0668633098848304 1103 1 0.26305820260267865 1103 2 0.3622589435857684 1103 3 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2214 7 -0.11111801480233652 2214 8 -0.11468791375757093 2214 9 -0.24243559041837415 2214 10 -0.2240669938690374 2214 11 -0.2690259641474313 2214 12 -0.38328935894431737 2214 13 -0.21806063684402233 2214 14 -0.3574990309191435 2214 15 -0.2742731515718546 2214 16 -0.4661013127656588 2214 17 -0.4123339084034541 2214 18 -0.32378393937985095 2214 19 -0.3238421434258001 2214 20 -0.11410904042898551 2214 21 -0.2461267191667389 2214 22 -0.08115960158204416 2214 23 -0.15944055806757973 2214 24 -0.26854618092433213 2214 25 -0.15356649056079874 2215 1 -0.4678622398113106 2215 2 -0.4880227570044475 2215 3 -0.47983522769659714 2215 4 -0.192162256786915 2215 5 -0.5543005156463607 2215 6 -0.6123190433416994 2215 7 -0.7106680483406649 2215 8 -0.8656477710273407 2215 9 -1.072040118984745 2215 10 -1.0792080405983242 2215 11 -1.2555062008142244 2215 12 -1.282260450456704 2215 13 -1.3809305347547038 2215 14 -1.5102103114104253 2215 15 -1.4541822163395821 2215 16 -1.3066118676121548 2215 17 -1.4451748489743608 2215 18 -1.2037345723664146 2215 19 -1.1900293373136037 2215 20 -1.168660818896413 2215 21 -1.3529251560144928 2215 22 -1.4365119997406381 2215 23 -1.4278045107868778 2215 24 -1.4708707833715664 2215 25 -1.643191548737292 2216 1 -1.6197651992684747 2216 2 -1.1328647316232328 2216 3 -2.8623319739644586 2216 4 -1.231894643460862 2216 5 -1.4446020070982044 2216 6 -1.310511344176092 2216 7 -1.4128241460523157 2216 8 -1.3617661071943843 2216 9 -1.4165078617580917 2216 10 -1.3633195786764674 2216 11 -1.293345666514206 2216 12 -1.3729886827429993 2216 13 -1.503082729391764 2216 14 -1.7943039486968644 2216 15 -1.748576881763256 2216 16 -1.694878284744128 2216 17 -1.8673285177942702 2216 18 -1.9500508702299726 2216 19 -1.8018752388746933 2216 20 -1.7606389476389026 2216 21 -1.8204520668713613 2216 22 -1.9084986647038065 2216 23 -1.9108229773840009 2216 24 -1.8994002930021878 2216 25 -1.770186434024808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26235.0 3 0.0 4 425.0 5 3187.0 6 3843.0 7 2950.0 8 2358.0 9 2228.0 10 1977.0 11 1817.0 12 1677.0 13 1606.0 14 1633.0 15 1604.0 16 1632.0 17 1747.0 18 2098.0 19 2507.0 20 3145.0 21 4258.0 22 5193.0 23 6437.0 24 7589.0 25 8671.0 26 9546.0 27 10402.0 28 11898.0 29 14321.0 30 17996.0 31 22906.0 32 28834.0 33 37923.0 34 50060.0 35 67837.0 36 98164.0 37 163307.0 38 545265.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.91581814129175 18.31303220539168 13.223758767431315 27.54739088588526 2 14.162698849268992 19.072643679337244 39.39349747006334 27.371160001330423 3 17.995169082125603 22.812620389405392 28.48255653401246 30.70965399445655 4 10.511167022934075 16.026067182828253 36.29538147886772 37.16738431536996 5 13.160725916175913 37.45554240819486 35.40444047371418 13.979291201915045 6 34.558138301013116 35.72272706656957 16.486431672296067 13.232702960121243 7 29.719773691558437 30.341030934161562 20.167634619941257 19.771560754338743 8 26.31999870402375 37.20074245797243 18.37839266545652 18.1008661725473 9 25.86629537387485 15.225022360272808 18.569385908892865 40.33929635695948 10 14.491650529129391 29.675908247872574 33.35441441195724 22.478026811040802 11 36.358801268282704 22.171337492755107 21.726841907879034 19.743019331083154 12 23.083801301787656 24.733880234171757 30.55237325641262 21.62994520762797 13 29.525964024306568 20.44876280812594 24.95974619432125 25.065526973246243 14 21.283706325713247 20.8951305721925 26.728454671986324 31.09270843010793 15 24.60957576015853 28.804329760712537 22.381283160384395 24.20481131874454 16 23.31045429050247 28.0679142734881 25.823313096802902 22.79831833920653 17 22.883595628228957 27.751965080393525 26.4471687496803 22.917270541697217 18 22.77253308929825 27.35347873662377 27.369598015878772 22.50439015819921 19 23.69143257600753 26.90130722464775 27.01691386537965 22.390346333965077 20 23.67158281597851 26.757301777245935 27.152519952679505 22.418595454096053 21 23.636552695188513 26.10067878316781 27.066010043672588 23.196758477971084 22 23.214060459772465 26.576611129862027 26.64044947650851 23.568878933856997 23 22.980879950156442 26.338331042018424 26.775655411239175 23.905133596585955 24 22.90979662840043 26.98293915046055 27.046095914754563 23.06116830638446 25 23.395623361956915 26.26093626813833 26.538620528009204 23.80481984189555 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 49.0 1 49.0 2 120.0 3 191.0 4 191.0 5 191.0 6 480.0 7 769.0 8 769.0 9 769.0 10 1222.5 11 1676.0 12 1676.0 13 1676.0 14 2791.0 15 3906.0 16 3906.0 17 3906.0 18 6926.5 19 9947.0 20 9947.0 21 9947.0 22 16044.0 23 22141.0 24 22141.0 25 22141.0 26 31545.0 27 40949.0 28 40949.0 29 40949.0 30 53119.0 31 65289.0 32 65289.0 33 65289.0 34 77243.5 35 89198.0 36 89198.0 37 89198.0 38 101771.0 39 114344.0 40 114344.0 41 114344.0 42 126232.0 43 138120.0 44 138120.0 45 138120.0 46 152922.0 47 167724.0 48 167724.0 49 167724.0 50 165401.5 51 163079.0 52 163079.0 53 163079.0 54 147638.5 55 132198.0 56 132198.0 57 132198.0 58 114920.5 59 97643.0 60 97643.0 61 97643.0 62 80385.5 63 63128.0 64 63128.0 65 63128.0 66 49464.0 67 35800.0 68 35800.0 69 35800.0 70 26213.0 71 16626.0 72 16626.0 73 16626.0 74 11605.0 75 6584.0 76 6584.0 77 6584.0 78 4602.0 79 2620.0 80 2620.0 81 2620.0 82 1787.5 83 955.0 84 955.0 85 955.0 86 599.5 87 244.0 88 244.0 89 244.0 90 166.0 91 88.0 92 88.0 93 88.0 94 47.0 95 6.0 96 6.0 97 6.0 98 4.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19799262918528973 2 0.06111094064823622 3 0.0 4 0.0 5 0.021137396486419224 6 0.40135483892962953 7 8.523143744523881E-4 8 0.035371046539774104 9 0.03724613816356936 10 0.0023864802484666863 11 0.0030683317480285966 12 0.010227772493428657 13 0.008096986557297686 14 0.0014489344365690596 15 8.52314374452388E-5 16 0.01278471561678582 17 0.025228505483790683 18 0.06537251252049817 19 0.02838206866926452 20 0.0 21 0.0 22 0.0 23 4.2615718722619405E-4 24 4.2615718722619405E-4 25 8.52314374452388E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1173276.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.30077168823841 #Duplication Level Percentage of deduplicated Percentage of total 1 85.649875826902 65.35151620594421 2 10.1336721354177 15.464140079359387 3 2.349419354600043 5.37787529325799 4 0.7861907571197893 2.3994784584960134 5 0.36244447763404436 1.3827396668809029 6 0.197468015383266 0.9040177174492878 7 0.12351417162690824 0.6596958626696635 8 0.0845525767503324 0.5161141483423494 9 0.05468000423940039 0.3754913867444158 >10 0.23515356471738033 3.0892727053829367 >50 0.012533895651575873 0.6592088456480776 >100 0.008373857412592667 1.4970541586572264 >500 0.001116506602594664 0.6679772628395847 >1k 0.0010048559423351975 1.6554182083278677 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 4298 0.36632471813963635 No Hit CCCATGTACTCTGCGTTGATACCAC 3840 0.327288719789717 No Hit GTATCAACGCAGAGTACTTTTTTTT 2546 0.21699923973557797 No Hit GGTATCAACGCAGAGTACTTTTTTT 1852 0.15784862214858225 No Hit TATCAACGCAGAGTACTTTTTTTTT 1838 0.15665538202434892 No Hit GAGTACATGGGAAGCAGTGGTATCA 1745 0.14872885834194172 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.52314374452388E-5 2 0.0 0.0 0.0 0.0 8.52314374452388E-5 3 0.0 0.0 0.0 0.0 8.52314374452388E-5 4 0.0 0.0 0.0 0.0 8.52314374452388E-5 5 0.0 0.0 0.0 0.0 8.52314374452388E-5 6 0.0 0.0 0.0 0.0 8.52314374452388E-5 7 0.0 0.0 0.0 0.0 8.52314374452388E-5 8 8.52314374452388E-5 0.0 0.0 0.0 8.52314374452388E-5 9 8.52314374452388E-5 0.0 0.0 0.0 8.52314374452388E-5 10 8.52314374452388E-5 0.0 0.0 0.0 8.52314374452388E-5 11 8.52314374452388E-5 0.0 0.0 0.0 8.52314374452388E-5 12 8.52314374452388E-5 0.0 0.0 0.0 2.556943123357164E-4 13 8.52314374452388E-5 0.0 0.0 8.52314374452388E-5 3.409257497809552E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGTGC 55 1.9844052E-4 13.797627 8 TGGCGAG 250 0.0 13.666059 18 GACGTGA 105 1.4006218E-9 13.55124 7 CTAGCAC 120 7.1304385E-10 12.694907 3 GGCGAGA 195 0.0 12.646746 19 GTCTAGA 115 5.0913513E-9 12.427439 1 GGACGTG 170 0.0 12.321527 6 TATGGCG 280 0.0 12.2054825 16 TAGGACG 165 0.0 12.117866 4 CTGGACG 55 0.0030169212 12.117866 4 TAGGACC 230 0.0 12.004966 4 GGCGAGG 95 1.0589774E-6 11.981127 19 GACCGTG 80 2.9089284E-5 11.857336 7 AGGACGT 170 0.0 11.761457 5 CTCCTAA 90 7.218203E-6 11.64497 1 CCCGTGC 90 7.5806565E-6 11.593839 8 GTTCTAG 165 5.456968E-12 11.548731 1 ATGGCGA 305 0.0 11.515793 17 GTAGGAC 340 0.0 11.481422 3 CTAGGAC 190 0.0 11.024524 3 >>END_MODULE