FastQCFastQC Report
Thu 26 May 2016
SRR1513085_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513085_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1892212
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC72420.3827266712186584No Hit
CCCATGTACTCTGCGTTGATACCAC57360.3031372806006938No Hit
GAGTACATGGGAAGCAGTGGTATCA32470.1715981084571919No Hit
GTATCAACGCAGAGTACTTTTTTTT22260.11764009529587593No Hit
GCGTTGATACCACTGCTTCCCATGT19560.1033710810416592No Hit
CATGTACTCTGCGTTGATACCACTG19530.10321253643883455No Hit
GTATCAACGCAGAGTACATGGGAAG19440.10273690263036066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTTA1250.015.9573813
TAGGACC4300.014.5820864
TAAACCG400.005278932414.2502985
CGTTTAT1450.013.75636214
GTAGGAC7500.013.2999263
TAGGACG4900.013.1842984
GGACGTG4750.012.9992416
TGTAGGA8900.012.7028492
AGGACGT5050.012.41610055
TATGGCG5050.012.22700716
CGCAGAT951.0412241E-611.9989818
CTAGGAC2700.011.9628973
TGGCGAG5100.011.735229518
AATCCCG1302.6157068E-911.69224319
GGCGAGA3600.011.61104719
ATAAGAC1604.5474735E-1111.2811883
ATGGCGA5400.011.25860117
TGCGTTT2050.011.12012512
ATTAGAC1201.27573E-711.0832723
GACGTGA2150.011.0455747
Produced by FastQC (version 0.11.5)