##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513085_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1892212
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.188783814921372	31.0	30.0	34.0	24.0	34.0
2	28.931712197153384	31.0	30.0	34.0	16.0	34.0
3	29.27108378976563	31.0	30.0	34.0	22.0	34.0
4	32.164036059384465	35.0	32.0	37.0	19.0	37.0
5	32.36195574280261	35.0	33.0	37.0	22.0	37.0
6	32.8298383056444	37.0	33.0	37.0	26.0	37.0
7	32.80795703652656	37.0	35.0	37.0	26.0	37.0
8	32.783566006346014	37.0	35.0	37.0	26.0	37.0
9	34.15042711916001	39.0	35.0	39.0	25.0	39.0
10	33.656488807808	38.0	34.0	39.0	19.0	39.0
11	34.01890274451277	39.0	34.0	39.0	25.0	39.0
12	33.84954117191943	39.0	34.0	39.0	23.0	39.0
13	33.94186327959024	39.0	34.0	39.0	24.0	39.0
14	34.2307643118213	39.0	33.0	41.0	16.0	41.0
15	34.77604359342399	40.0	34.0	41.0	22.0	41.0
16	34.35792025417871	39.0	34.0	41.0	17.0	41.0
17	33.892970766489164	39.0	33.0	41.0	11.0	41.0
18	33.30073638683192	39.0	32.0	41.0	10.0	41.0
19	34.19288060745836	39.0	33.0	41.0	18.0	41.0
20	34.452839322443786	39.0	34.0	41.0	18.0	41.0
21	34.47729852680355	39.0	34.0	41.0	18.0	41.0
22	34.06823389768166	39.0	34.0	41.0	10.0	41.0
23	32.37553720196257	39.0	31.0	41.0	2.0	41.0
24	33.4571506786766	39.0	33.0	41.0	2.0	41.0
25	33.196490668064676	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.780458184562363
1101	2	1.074669662263691
1101	3	0.8073628575653942
1101	4	0.8052477294496043
1101	5	1.1183083451541904
1101	6	0.7711065975600917
1101	7	0.7178614655438125
1101	8	0.6948819002765987
1101	9	0.8427781187968293
1101	10	1.1317958447353362
1101	11	0.8485648505933696
1101	12	0.8807393394351806
1101	13	0.9125301585856462
1101	14	-0.008342313122888356
1101	15	0.6505547174417501
1101	16	1.2670118819935752
1101	17	1.838060793950902
1101	18	-4.263858161200293
1101	19	-0.655318941736958
1101	20	0.2736909871732749
1101	21	0.6341419816946825
1101	22	1.1667225646058057
1101	23	3.000651242009802
1101	24	1.9552881174522057
1101	25	2.1497225058778184
1102	1	1.114816114787967
1102	2	1.5196211907078627
1102	3	1.2878425425771383
1102	4	1.8796036996568262
1102	5	1.5951885981948237
1102	6	1.189295844236689
1102	7	1.1490109259803631
1102	8	1.0469118682299197
1102	9	1.244278850415732
1102	10	1.619865769959759
1102	11	1.3240473670913389
1102	12	1.4534776670676877
1102	13	1.400048593361639
1102	14	2.2193864760462603
1102	15	1.6206215224474008
1102	16	1.9811592620751455
1102	17	1.597522368618634
1102	18	2.0320849144449937
1102	19	1.7782223183440564
1102	20	1.6879443864311554
1102	21	1.7618455867607636
1102	22	2.1465254660604245
1102	23	3.720396344253878
1102	24	2.666179884408777
1102	25	2.98249378741739
1103	1	0.8365744238389219
1103	2	1.160411758797224
1103	3	0.8865230421894417
1103	4	1.292737492321642
1103	5	1.274203467512784
1103	6	0.872522538061375
1103	7	0.8126775807385584
1103	8	0.7131375441320245
1103	9	0.8968189270291234
1103	10	1.2219267235464173
1103	11	1.0085789189175856
1103	12	1.109805895021971
1103	13	0.9157083989047194
1103	14	1.7323882608072125
1103	15	1.195066986100315
1103	16	1.5072206966968906
1103	17	1.873001001049957
1103	18	2.072316379876135
1103	19	1.3983195719657857
1103	20	1.3957277706941653
1103	21	1.273850079105209
1103	22	1.4756966214654526
1103	23	3.05537048861774
1103	24	1.9371143453903414
1103	25	2.267222910821509
1104	1	0.5376251803816707
1104	2	0.9048266542418659
1104	3	0.7373994989011408
1104	4	1.235205540624932
1104	5	1.1696841885083842
1104	6	0.7751809172643789
1104	7	0.6951260638735448
1104	8	0.6311702384805074
1104	9	0.8398175811261765
1104	10	1.0370805501418872
1104	11	0.6193523271738357
1104	12	0.802770435240511
1104	13	0.7010030102862856
1104	14	1.5044835008658382
1104	15	1.0916317430769737
1104	16	1.4338802755303846
1104	17	1.793453817383778
1104	18	2.555105560033738
1104	19	1.4795184563683321
1104	20	1.0137468194912458
1104	21	1.127345450907221
1104	22	1.4256218654410375
1104	23	2.849375919350855
1104	24	1.9729982188028146
1104	25	1.915350034349089
1105	1	0.45728866384837374
1105	2	0.8358866144578379
1105	3	0.6305220676217225
1105	4	0.7311347221867308
1105	5	0.8192587668478168
1105	6	0.5117939256390827
1105	7	0.2805788639193878
1105	8	0.3589562548478398
1105	9	0.6418707809227158
1105	10	1.1289381968663932
1105	11	0.8072337201402249
1105	12	0.5921285655344022
1105	13	0.6726615458232601
1105	14	1.3800244418799252
1105	15	0.7418565729918853
1105	16	1.1485655017095127
1105	17	1.7659954342010096
1105	18	1.5190919388990096
1105	19	1.230195811709443
1105	20	0.8700880260505954
1105	21	0.8390850828213701
1105	22	1.353015772705561
1105	23	2.8604729412798378
1105	24	1.7828427363199708
1105	25	1.8104644313440375
1106	1	-0.5713299610952092
1106	2	-0.20075788682093432
1106	3	-0.48299801662680864
1106	4	-2.554602602922344
1106	5	-0.5150887583515207
1106	6	-0.5866161841848978
1106	7	-0.6922196918200427
1106	8	-0.5457020872613825
1106	9	-0.5393791062444251
1106	10	-0.43014650299820545
1106	11	-0.5106269688924172
1106	12	-0.45494108994927274
1106	13	-0.5429473002727221
1106	14	0.21584029865741883
1106	15	-0.32382719412109395
1106	16	-1.2101142034206163
1106	17	-6.093770475231818
1106	18	-7.441799892002152
1106	19	-3.406154421478462
1106	20	-1.9660792674652754
1106	21	-1.4047704911217664
1106	22	-1.7340358295210052
1106	23	-5.124901513361358
1106	24	-2.587377212529134
1106	25	-1.3178563148572593
1107	1	0.5845146876205405
1107	2	0.9564248637480368
1107	3	0.6158212426762368
1107	4	0.9252822432337737
1107	5	1.0084034533944077
1107	6	0.595553531772893
1107	7	0.5779363097169181
1107	8	0.6300965708534747
1107	9	0.39500602070263824
1107	10	0.9092500624851922
1107	11	0.6066484722449346
1107	12	0.7107080691557428
1107	13	0.6374365852827992
1107	14	1.4614096540205281
1107	15	0.9827521810451643
1107	16	-5.988424846875233
1107	17	-1.0386498533537676
1107	18	0.7600465892439701
1107	19	0.8673442336807042
1107	20	0.7401086555684486
1107	21	0.7357406075097472
1107	22	0.7308814997137674
1107	23	2.6419023763321405
1107	24	1.678647910871092
1107	25	1.3800531817880284
1108	1	0.5188914673690164
1108	2	0.7518564818663123
1108	3	0.5572184591877516
1108	4	1.1210890120916872
1108	5	0.8740451883099425
1108	6	0.4881948797343014
1108	7	0.5282274874208568
1108	8	0.5263765548121242
1108	9	0.5588343979803341
1108	10	0.9907165787505292
1108	11	0.4970265257272004
1108	12	0.6607806611875873
1108	13	0.5061934300179871
1108	14	1.1295401680875656
1108	15	0.7733347875390919
1108	16	1.1003645827299735
1108	17	1.0864140034642844
1108	18	0.6655180831145344
1108	19	0.8546244952419855
1108	20	0.627995442504556
1108	21	0.8168918582351381
1108	22	1.2873826693759014
1108	23	2.7828446108150757
1108	24	1.5754943637641787
1108	25	1.7510801261386746
1109	1	0.3427534218708388
1109	2	0.6196899988252831
1109	3	0.5492688088645536
1109	4	0.5465421260830183
1109	5	0.3615526948944563
1109	6	0.3230467103952108
1109	7	0.25167191032409164
1109	8	0.20306086687798341
1109	9	0.3816607984607572
1109	10	0.8428892325586688
1109	11	0.522541583817528
1109	12	0.5711184693121893
1109	13	0.2712063626218608
1109	14	-0.9014622623908224
1109	15	0.04311741576574235
1109	16	-0.31088370907875174
1109	17	0.8679317284608601
1109	18	1.5335193456392986
1109	19	0.8360224695280891
1109	20	0.61048594992603
1109	21	0.6391431479491132
1109	22	0.492244099007884
1109	23	-4.070039690847956
1109	24	-1.3090186657751133
1109	25	-0.1197258800746468
1110	1	0.46390625346683123
1110	2	0.7353788416275897
1110	3	0.5176882816583088
1110	4	0.8629932166977241
1110	5	0.6010072056186857
1110	6	0.3961752009990036
1110	7	0.3503684483728122
1110	8	0.32139347252844175
1110	9	0.21762106697573813
1110	10	0.5098801729068683
1110	11	0.2345829162048716
1110	12	0.3234406872895974
1110	13	0.20811534768616724
1110	14	1.0274035528868808
1110	15	0.43230768664569297
1110	16	0.8240614418244903
1110	17	1.2554574247624117
1110	18	1.691686221514793
1110	19	0.9085456189395771
1110	20	0.5534142367786146
1110	21	0.35801696773844327
1110	22	0.7037096523197448
1110	23	1.9275621392744782
1110	24	1.1296476734342562
1110	25	1.3371466298855097
1111	1	0.6114006133765884
1111	2	0.8837132149775613
1111	3	0.575954547251893
1111	4	1.0379506826838494
1111	5	0.8262961435750213
1111	6	0.38892575167448484
1111	7	0.3264733993853639
1111	8	0.18400060757593906
1111	9	0.34240973026101784
1111	10	0.5382446267381269
1111	11	0.2512151823211326
1111	12	0.3907870981655037
1111	13	0.308667623189244
1111	14	-0.16386484074075014
1111	15	0.20427070472064912
1111	16	0.5943711001123546
1111	17	1.1815029081568085
1111	18	1.7777102645661529
1111	19	0.9512729692438455
1111	20	0.7941874224829206
1111	21	0.648284278682091
1111	22	0.9213064672021218
1111	23	0.8194924101588086
1111	24	0.7873155294532879
1111	25	0.5047386446910807
1112	1	0.4526671442136525
1112	2	0.7911071047844587
1112	3	0.5707397243562262
1112	4	1.0291908319181573
1112	5	0.8785441948123776
1112	6	0.2666078070152409
1112	7	0.33445650802943305
1112	8	0.30502309076211986
1112	9	0.3048235221351234
1112	10	0.739990357998721
1112	11	0.31976902485504866
1112	12	0.44375039386940074
1112	13	0.21915065607533535
1112	14	0.7113238857586737
1112	15	0.25317159010862866
1112	16	0.7446878575994731
1112	17	0.996915913930458
1112	18	1.0528757289091004
1112	19	0.6232226409889776
1112	20	0.4682422748261601
1112	21	0.4201917042119945
1112	22	0.5011108155319661
1112	23	1.9863823400514846
1112	24	1.0060470912976456
1112	25	1.0892288469559759
1113	1	0.37362897216246793
1113	2	0.7311941665161825
1113	3	0.4481358226525707
1113	4	0.5466125187174882
1113	5	0.7539754305980466
1113	6	0.23539558969977747
1113	7	0.2140051468209947
1113	8	0.12613903622255407
1113	9	0.23798496212921805
1113	10	0.6060342190388184
1113	11	0.3789283187166319
1113	12	0.3993481504223837
1113	13	0.2369508485321603
1113	14	0.9737198645840834
1113	15	0.47001634088138644
1113	16	0.8797395450721837
1113	17	1.4023135023552484
1113	18	1.9425547430783539
1113	19	0.8844364005656544
1113	20	0.6637078096323918
1113	21	0.6761668803230165
1113	22	0.9567751499810839
1113	23	0.7827464049307196
1113	24	0.815401991669745
1113	25	1.314572651736114
1114	1	-0.5178117623574394
1114	2	-3.1073042827571804
1114	3	-1.287140360002919
1114	4	-0.0749081122211237
1114	5	0.2052272741697152
1114	6	-0.03322040187006792
1114	7	-0.048491000645832116
1114	8	-0.05928807122466395
1114	9	-0.03571159529439427
1114	10	0.20480917912210828
1114	11	-0.053331940835157354
1114	12	-0.03373601305418106
1114	13	-0.17767706895774182
1114	14	0.693110641921578
1114	15	0.004799609310147446
1114	16	0.3774744393355718
1114	17	0.7260309829061882
1114	18	0.9610271605160037
1114	19	0.39661576085875794
1114	20	0.14185865671714737
1114	21	0.03345041258795334
1114	22	0.08448918412280193
1114	23	1.8387260877077551
1114	24	0.8004367839164814
1114	25	0.6997060478300838
1115	1	0.09966796851141524
1115	2	0.35751126431778246
1115	3	0.12472869144772503
1115	4	0.2796775903143711
1115	5	0.16031220783204958
1115	6	-0.12182391365349332
1115	7	-0.2336662066578583
1115	8	-0.23613134882107545
1115	9	-0.23634528981930458
1115	10	0.14969614710530976
1115	11	-0.05359453272954795
1115	12	-0.19158429215769246
1115	13	-0.3388175556212403
1115	14	0.6413503055587313
1115	15	0.06299130139305475
1115	16	0.5047153341915305
1115	17	0.8058899802547259
1115	18	1.2906611155892946
1115	19	0.5211333632006259
1115	20	0.003104997545072763
1115	21	-0.005421974903683235
1115	22	0.3921919137502172
1115	23	2.12391008084154
1115	24	0.7769043609698301
1115	25	0.47225824690947604
1116	1	0.09680106353416917
1116	2	-0.05092464305692701
1116	3	0.08273980303737005
1116	4	0.6899988310606986
1116	5	0.3214270036649651
1116	6	-0.005345837748230053
1116	7	-0.05052701509539048
1116	8	-0.013364170600176806
1116	9	-0.008397679863854535
1116	10	0.40626418764681915
1116	11	-0.006720086035990391
1116	12	0.0790120106785821
1116	13	-0.20576655811440503
1116	14	0.4874017672448048
1116	15	0.008282753317004676
1116	16	0.3590201898892076
1116	17	0.6524616098109774
1116	18	1.1645513683037834
1116	19	0.41173445138989706
1116	20	0.24486189204239395
1116	21	0.10109290036609053
1116	22	0.3495532701106683
1116	23	1.6330850381322648
1116	24	0.7294865852976073
1116	25	0.9020483134737347
1201	1	0.9569027937719916
1201	2	1.3723630934614555
1201	3	1.0902549534546715
1201	4	1.5768545097230486
1201	5	1.489945016606491
1201	6	0.9952624001280981
1201	7	0.9241933205578903
1201	8	0.8468695367978327
1201	9	1.0988144622074003
1201	10	1.4604441544627633
1201	11	1.1209473297951504
1201	12	1.3488626047698915
1201	13	1.1923971047779744
1201	14	2.072257043074913
1201	15	1.5237345400332885
1201	16	1.926052618747697
1201	17	2.358538662045042
1201	18	2.932515465340636
1201	19	2.1081249838832363
1201	20	1.7983614864067121
1201	21	1.6712746665569043
1201	22	1.9589362423829542
1201	23	3.5787741724009123
1201	24	2.500536079751761
1201	25	2.770581702185723
1202	1	0.8872890446308936
1202	2	1.23974971454345
1202	3	0.92847431749367
1202	4	1.5950769513367362
1202	5	1.3788567587727698
1202	6	0.9706787487533219
1202	7	0.7647322922518214
1202	8	0.8017164122215732
1202	9	0.8725932973301411
1202	10	1.4635518192996386
1202	11	1.1349342973328262
1202	12	1.1730599595792128
1202	13	0.944371247056381
1202	14	1.7600469940317751
1202	15	1.3289023516491483
1202	16	1.5663645050419746
1202	17	2.0590407485492648
1202	18	2.546256226903253
1202	19	1.7064470200797786
1202	20	1.3913609942994754
1202	21	1.3605337023618986
1202	22	1.8609093943526815
1202	23	3.296647035048146
1202	24	2.059948125866633
1202	25	2.315391265825241
1203	1	1.089631962628765
1203	2	1.4129836513556633
1203	3	1.0683394222150042
1203	4	1.7157727269845893
1203	5	1.4874829568558354
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2211	18	-5.497244714742397
2211	19	-1.9287674635079313
2211	20	-1.104300787828052
2211	21	-0.8961274011372637
2211	22	-0.4513085067445388
2211	23	-4.7399797336647325
2211	24	-2.0174806852845926
2211	25	-1.443642907732098
2212	1	0.37123067810229315
2212	2	0.6201135446665411
2212	3	0.45158315154161954
2212	4	0.8472206263081148
2212	5	0.8625612576867354
2212	6	0.24683694035947212
2212	7	0.1905770305431247
2212	8	0.06790173859229043
2212	9	0.08854181056075561
2212	10	-3.4429396677840245
2212	11	-0.9615533970264423
2212	12	-0.1707615780955436
2212	13	0.12572525680889868
2212	14	0.6271227178100176
2212	15	0.3675967116322667
2212	16	0.6218000647973838
2212	17	1.1038913216363468
2212	18	1.7285909166176836
2212	19	0.912347313569505
2212	20	0.4362237098187194
2212	21	0.2823625097822102
2212	22	0.4428022423887157
2212	23	1.6559691016329552
2212	24	0.8582323287766727
2212	25	0.7586611085236044
2213	1	-1.3240750880332364
2213	2	-3.5633822456285706
2213	3	-1.590192259983695
2213	4	-3.146944764842381
2213	5	-1.069349916904848
2213	6	-0.9037027216738807
2213	7	-0.7455271318188821
2213	8	-0.6582313978802503
2213	9	-0.574632649961103
2213	10	-0.6544704008698119
2213	11	-0.6727909118461639
2213	12	-0.5673772308020801
2213	13	-0.9120470734170567
2213	14	-5.935728299894752
2213	15	-2.5687879639854145
2213	16	-1.7832547238881986
2213	17	-0.5915007048328675
2213	18	0.2114618878756076
2213	19	-0.5383358482127534
2213	20	-0.6921644322607463
2213	21	-0.8790917592015859
2213	22	-0.7727285629150273
2213	23	-5.026904143153139
2213	24	-2.9083171174520466
2213	25	-2.058135650762587
2214	1	-0.19243370961106976
2214	2	-0.006578772216990103
2214	3	-0.14256278170850933
2214	4	0.07558714095734587
2214	5	-0.1732999869374865
2214	6	-0.41306939841003754
2214	7	-0.516212907175948
2214	8	-0.5188493705768096
2214	9	-0.43343530571296185
2214	10	0.08009864880820317
2214	11	-0.5141792954599538
2214	12	-0.3258710384990593
2214	13	-0.5624309888575567
2214	14	-0.011924613424902475
2214	15	-0.31834294014925035
2214	16	0.13404387878664892
2214	17	0.3410457174520758
2214	18	0.8685047385174371
2214	19	-0.09441868336572412
2214	20	-0.13876632575667713
2214	21	-0.38586575883251584
2214	22	0.1441697451948869
2214	23	1.5840747969230335
2214	24	0.5559705570191227
2214	25	0.352088164138614
2215	1	-2.1904945456787033
2215	2	-4.28813729874609
2215	3	-2.2567984639564713
2215	4	-3.6146316247978554
2215	5	-4.736465988180772
2215	6	-2.0903837909712166
2215	7	-1.3724708510584875
2215	8	-1.7366724653094963
2215	9	-1.3037201678340864
2215	10	-2.7506348560613247
2215	11	-1.6163516974942382
2215	12	-1.4660912726998063
2215	13	-1.3788675446674645
2215	14	-0.7737027704604671
2215	15	-1.2782486659447798
2215	16	-1.978121295232306
2215	17	-6.274187691968493
2215	18	-2.517000153853658
2215	19	-1.7290249686390098
2215	20	-1.4215403988820015
2215	21	-1.3345815216165562
2215	22	-1.0778612618487529
2215	23	-6.022033039191918
2215	24	-3.175397724230624
2215	25	-2.9691926303883243
2216	1	-1.1251485425566372
2216	2	-0.6770204719278894
2216	3	-0.9279434945660157
2216	4	-3.0767328768729136
2216	5	-1.3317647890168303
2216	6	-1.1844681114888154
2216	7	-1.3326819230965121
2216	8	-1.154832736486707
2216	9	-1.1953657035746161
2216	10	-0.8419320602812164
2216	11	-1.4109389830456394
2216	12	-2.000511346744428
2216	13	-1.5484666269225897
2216	14	-7.128392907797306
2216	15	-3.633469074783722
2216	16	-1.9583956594866692
2216	17	-1.0919216573472923
2216	18	-0.3969035214451395
2216	19	-0.8429351001896563
2216	20	-1.4725288466214437
2216	21	-1.6578320897922225
2216	22	-1.0975849637085346
2216	23	0.08379499658342127
2216	24	-0.6346592583126522
2216	25	-0.4659959170660173
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91859.0
3	4.0
4	813.0
5	4660.0
6	5684.0
7	5193.0
8	4887.0
9	4569.0
10	4423.0
11	4219.0
12	4075.0
13	4001.0
14	4010.0
15	4233.0
16	4286.0
17	4488.0
18	5226.0
19	5966.0
20	7639.0
21	9296.0
22	11674.0
23	14182.0
24	16880.0
25	19583.0
26	22236.0
27	25370.0
28	28076.0
29	34722.0
30	40843.0
31	46226.0
32	59305.0
33	77643.0
34	100568.0
35	131820.0
36	184890.0
37	257072.0
38	641591.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.47164959635476	17.148494253518205	11.998988373704913	25.38086777642212
2	14.381105915980589	18.535019016010672	41.66988755359406	25.413987514414682
3	20.341609406629704	22.717162996684674	27.91293971981219	29.02828787687343
4	10.922748267226892	15.185547210567021	38.083421573349	35.80828294885709
5	12.923485870984011	37.297208137526546	35.76257987568813	14.016726115801308
6	34.52062600333754	34.9396953501169	17.073217144841635	13.466461501703922
7	30.109709458905865	30.29207401357955	20.186646157261677	19.411570370252907
8	26.532378238339234	36.346840939257795	18.89114253529406	18.229638287108912
9	26.53918373992679	15.357105852450662	18.77507067896209	39.328639728660455
10	14.597779173460044	28.851448654752616	33.89657571470563	22.654196457081714
11	36.109332968248744	22.278159571920693	22.00778201066815	19.604725449162412
12	23.62583312674204	24.321407069069505	30.560904610853363	21.49185519333509
13	29.642178306161238	20.498580257223985	24.697498588714367	25.16174284790041
14	21.18964767175163	20.85065601994305	27.388495639308232	30.57120066899709
15	24.860858924243505	28.51307098851085	22.37992160842802	24.246148478817624
16	23.220661967401156	27.535898635415755	26.525160100117084	22.718279297065997
17	22.835203430561474	27.347753504516035	27.17103433838945	22.646008726533037
18	22.488040553755397	26.768385831558138	28.46152626320825	22.282047351478216
19	23.945061800188203	26.778566066462954	26.807219361591894	22.469152771756946
20	23.872886422516213	26.488180035807986	27.10956005448903	22.52937348718678
21	23.592910539089356	26.136981782599662	27.00072370566906	23.269383972641915
22	23.462428668553684	26.431836373037164	26.511084724364824	23.59465023404433
23	22.004999838766036	26.04747890874739	28.445583008794916	23.501938243691654
24	23.24807291721739	26.900753914965158	26.713332557918225	23.137840609899232
25	23.45140509341738	25.98035121255983	26.73726936411051	23.830974329912276
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	148.0
1	148.0
2	266.0
3	384.0
4	384.0
5	384.0
6	721.5
7	1059.0
8	1059.0
9	1059.0
10	1792.0
11	2525.0
12	2525.0
13	2525.0
14	4374.5
15	6224.0
16	6224.0
17	6224.0
18	11119.0
19	16014.0
20	16014.0
21	16014.0
22	25473.5
23	34933.0
24	34933.0
25	34933.0
26	50020.0
27	65107.0
28	65107.0
29	65107.0
30	85089.0
31	105071.0
32	105071.0
33	105071.0
34	125119.0
35	145167.0
36	145167.0
37	145167.0
38	166118.5
39	187070.0
40	187070.0
41	187070.0
42	206354.0
43	225638.0
44	225638.0
45	225638.0
46	248348.5
47	271059.0
48	271059.0
49	271059.0
50	265571.0
51	260083.0
52	260083.0
53	260083.0
54	235739.0
55	211395.0
56	211395.0
57	211395.0
58	183782.5
59	156170.0
60	156170.0
61	156170.0
62	129259.0
63	102348.0
64	102348.0
65	102348.0
66	80001.5
67	57655.0
68	57655.0
69	57655.0
70	42242.0
71	26829.0
72	26829.0
73	26829.0
74	18919.5
75	11010.0
76	11010.0
77	11010.0
78	7645.0
79	4280.0
80	4280.0
81	4280.0
82	2845.0
83	1410.0
84	1410.0
85	1410.0
86	942.5
87	475.0
88	475.0
89	475.0
90	298.5
91	122.0
92	122.0
93	122.0
94	75.5
95	29.0
96	29.0
97	29.0
98	18.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1155261672582142
2	0.04814471105774617
3	0.03672949965437276
4	0.031233286756452237
5	0.03588392843930807
6	0.021192128577558964
7	0.020980735773792787
8	0.024468717035934662
9	0.029594992527264385
10	0.035091205425184915
11	0.021826306988857486
12	0.020769342970026614
13	0.016911424301293936
14	0.022460485400156008
15	0.022566181802039096
16	0.022566181802039096
17	0.02695258248018721
18	0.021244976778500506
19	0.033400062995055525
20	0.024045931428402313
21	0.029542144326322847
22	0.03646525864966505
23	0.029119358718790494
24	0.039741847108040754
25	0.03139183135927687
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1892212.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.24301912057184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.28874583426257	60.17030457833079
2	11.105369973071642	16.04570910611278
3	2.927696992610544	6.3451700944921265
4	1.1346620747136689	3.278856558357092
5	0.5246177677985294	1.894998571503044
6	0.27716950425338965	1.201413707725023
7	0.178760066077785	0.9039916810156476
8	0.11642768587801688	0.6728870029639605
9	0.0763110782776354	0.49616484184104015
>10	0.341596227983907	4.194985302111682
>50	0.016538982610354934	0.8160147403563507
>100	0.009208847868488804	1.3155839572840322
>500	0.0017815166729367212	0.8072305664922097
>1k	9.649881978407172E-4	1.1607324527817562
>5k	1.4845972274472573E-4	0.6959568386325541
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	7242	0.3827266712186584	No Hit
CCCATGTACTCTGCGTTGATACCAC	5736	0.3031372806006938	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3247	0.1715981084571919	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2226	0.11764009529587593	No Hit
GCGTTGATACCACTGCTTCCCATGT	1956	0.1033710810416592	No Hit
CATGTACTCTGCGTTGATACCACTG	1953	0.10321253643883455	No Hit
GTATCAACGCAGAGTACATGGGAAG	1944	0.10273690263036066	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	5.284820094154355E-5	0.0	0.0	0.0
6	0.0	5.284820094154355E-5	0.0	0.0	0.0
7	0.0	5.284820094154355E-5	0.0	5.284820094154355E-5	0.0
8	0.0	5.284820094154355E-5	0.0	5.284820094154355E-5	0.0
9	0.0	5.284820094154355E-5	0.0	5.284820094154355E-5	0.0
10	0.0	5.284820094154355E-5	0.0	5.284820094154355E-5	0.0
11	0.0	5.284820094154355E-5	0.0	5.284820094154355E-5	0.0
12	0.0	5.284820094154355E-5	0.0	1.056964018830871E-4	5.284820094154355E-5
13	0.0	5.284820094154355E-5	0.0	1.056964018830871E-4	5.284820094154355E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTTA	125	0.0	15.95738	13
TAGGACC	430	0.0	14.582086	4
TAAACCG	40	0.0052789324	14.250298	5
CGTTTAT	145	0.0	13.756362	14
GTAGGAC	750	0.0	13.299926	3
TAGGACG	490	0.0	13.184298	4
GGACGTG	475	0.0	12.999241	6
TGTAGGA	890	0.0	12.702849	2
AGGACGT	505	0.0	12.4161005	5
TATGGCG	505	0.0	12.227007	16
CGCAGAT	95	1.0412241E-6	11.998981	8
CTAGGAC	270	0.0	11.962897	3
TGGCGAG	510	0.0	11.7352295	18
AATCCCG	130	2.6157068E-9	11.692243	19
GGCGAGA	360	0.0	11.611047	19
ATAAGAC	160	4.5474735E-11	11.281188	3
ATGGCGA	540	0.0	11.258601	17
TGCGTTT	205	0.0	11.120125	12
ATTAGAC	120	1.27573E-7	11.083272	3
GACGTGA	215	0.0	11.045574	7
>>END_MODULE