FastQCFastQC Report
Thu 26 May 2016
SRR1513085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513085_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1892212
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC83010.438692916015753No Hit
CCCATGTACTCTGCGTTGATACCAC76250.40296753217926956No Hit
GTATCAACGCAGAGTACTTTTTTTT35500.1876111133424796No Hit
GAGTACATGGGAAGCAGTGGTATCA35000.1849687032954024No Hit
TATCAACGCAGAGTACTTTTTTTTT23840.12599011104463984No Hit
CATGTACTCTGCGTTGATACCACTG22880.12091668375425164No Hit
GGTATCAACGCAGAGTACTTTTTTT22720.12007111253918694No Hit
GTATCAACGCAGAGTACATGGGAAG21850.11547331905727265No Hit
GCGTTGATACCACTGCTTCCCATGT19810.10469228606519777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG755.6845238E-815.2350625
TATACCG400.005199567414.282875
ACGGCGT400.005329040314.2300188
GGACCGC852.6253656E-713.4427016
GAACCGA500.001473939513.3306796
TCCAACG1800.013.17942618
TAGGACC3900.012.4517334
GGACCGT853.844265E-612.3224766
CACCGTT701.1055275E-412.1971597
CTACTAG951.0012809E-612.0378991
CCAACGA1353.8016879E-1011.94555619
CGTTTCC1157.162453E-811.55510914
GTAGGAC7550.011.4767433
GTCCTAC7400.011.3329771
TAGGACG5400.011.2852314
TGGCGAG5600.011.18368418
CTGATCG600.00593931911.0674999
GGCGAGG2500.011.00396519
GGACGTG4950.010.77226456
AGGACGT5500.010.7337945
Produced by FastQC (version 0.11.5)