FastQCFastQC Report
Thu 26 May 2016
SRR1513084_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513084_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685311
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC31190.1850696993017906No Hit
GTATCAACGCAGAGTACTTTTTTTT26560.15759702511880597No Hit
CCCATGTACTCTGCGTTGATACCAC24330.1443650459766773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACAC2200.012.5231573
GCGATGC752.0762102E-411.398713
GTCCTAC4450.011.113711
TGGACCG600.00587320511.0840435
CCGTGCG600.005880995711.0820699
GCGCCAC600.005880995711.08206913
TAGGACC2500.011.0207064
CTAGTAC1053.4647437E-610.8575173
GACTATC700.001492565410.8562277
CTATACT2600.010.5968334
GTGTTAG2250.010.5675241
CTAAGAC1803.45608E-1110.5559193
GCGTTTA909.526253E-510.55435213
ATAGGAC1909.094947E-1210.5003613
TAGCCCT4700.010.3091714
GTCCTAG2400.010.3033351
TAGGACT2600.010.2314254
TTAGACA1409.6770236E-810.1792234
GACTGCG750.002649622510.1324797
TATACTG3400.010.0594675
Produced by FastQC (version 0.11.5)