##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513084_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1685311
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.36892419262676	31.0	30.0	34.0	25.0	34.0
2	29.112396465696836	31.0	30.0	34.0	16.0	34.0
3	29.450324005480294	31.0	30.0	34.0	23.0	34.0
4	32.364076422689934	35.0	33.0	37.0	19.0	37.0
5	32.5594302772604	35.0	33.0	37.0	23.0	37.0
6	33.03301111782929	37.0	35.0	37.0	27.0	37.0
7	33.01399088951535	37.0	35.0	37.0	26.0	37.0
8	32.99434288389502	37.0	35.0	37.0	26.0	37.0
9	34.37538056774091	39.0	35.0	39.0	27.0	39.0
10	33.87989219793854	39.0	34.0	39.0	22.0	39.0
11	34.24396506045472	39.0	35.0	39.0	26.0	39.0
12	34.07470134592369	39.0	34.0	39.0	25.0	39.0
13	34.16522885093612	39.0	35.0	39.0	25.0	39.0
14	34.46866068043228	40.0	34.0	41.0	17.0	41.0
15	35.01879415728017	40.0	34.0	41.0	25.0	41.0
16	34.61472274256799	39.0	34.0	41.0	18.0	41.0
17	34.14799879666127	39.0	33.0	41.0	17.0	41.0
18	33.54370142958777	39.0	32.0	41.0	10.0	41.0
19	34.45577107133342	39.0	34.0	41.0	18.0	41.0
20	34.70907684101035	39.0	34.0	41.0	19.0	41.0
21	34.742762611767205	40.0	34.0	41.0	18.0	41.0
22	34.32697585193475	40.0	34.0	41.0	15.0	41.0
23	32.63515635986474	39.0	31.0	41.0	8.0	41.0
24	33.72658577556309	39.0	33.0	41.0	2.0	41.0
25	33.472055306112644	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7000162594439274
1101	2	1.1089908446031451
1101	3	0.9349458990699766
1101	4	0.8565825587607634
1101	5	1.0457332385027058
1101	6	0.7453248157989592
1101	7	0.7846214619285163
1101	8	0.7390018315810352
1101	9	0.8300067435699816
1101	10	1.1699567590564044
1101	11	0.8397394906706879
1101	12	0.9131787168508936
1101	13	0.808392401018736
1101	14	0.08983688410490487
1101	15	0.5422466381517381
1101	16	1.2224551025137202
1101	17	1.7626696715986938
1101	18	-4.612832428680115
1101	19	-0.8070946474166405
1101	20	0.25172394385302255
1101	21	0.5703161733037376
1101	22	1.1262209565277743
1101	23	3.0757659915442943
1101	24	1.936985509774253
1101	25	2.158684450534764
1102	1	0.825275402358244
1102	2	0.9952250771947746
1102	3	0.7517220275594347
1102	4	1.3011692782513578
1102	5	1.2297267550046413
1102	6	0.8946259005796549
1102	7	0.7767578255297636
1102	8	0.5224145218387122
1102	9	0.7137392050201328
1102	10	1.0993473550484083
1102	11	0.7585936297336815
1102	12	0.9436153099858942
1102	13	0.6933409046456163
1102	14	1.5987448179688926
1102	15	1.0380308965089426
1102	16	1.2591389385009961
1102	17	0.9936025706386076
1102	18	1.3289588617075125
1102	19	1.1027737588959567
1102	20	1.0780048248732612
1102	21	1.1139428066361177
1102	22	1.5802717907991521
1102	23	3.164649403693744
1102	24	2.1658091087182854
1102	25	2.3028620884925886
1103	1	0.41801335075414414
1103	2	0.7008565982496151
1103	3	0.49384467481757355
1103	4	0.8596652312287318
1103	5	0.7750390475981348
1103	6	0.4060011120130085
1103	7	0.35894440542110573
1103	8	0.2788411254525087
1103	9	0.38881751007567544
1103	10	0.670062855606929
1103	11	0.4669785389619179
1103	12	0.5887550403603399
1103	13	0.41964837589883075
1103	14	1.0912231079650212
1103	15	0.6494417381980497
1103	16	0.9227773698071857
1103	17	1.3249212549428933
1103	18	1.3047810550874246
1103	19	0.7642413062535383
1103	20	0.6443933064421756
1103	21	0.7188215900220172
1103	22	1.0187023207273995
1103	23	2.51831827208769
1103	24	1.2631956799155333
1103	25	1.4641100323784286
1104	1	0.5404965812280516
1104	2	0.8546700116948678
1104	3	0.5049506124424887
1104	4	1.1374117280559233
1104	5	0.9026768169481798
1104	6	0.47972558848097435
1104	7	0.43118333039416257
1104	8	0.34282724191968583
1104	9	0.4619538025307506
1104	10	0.9256224508738171
1104	11	0.5636000584071539
1104	12	0.6939446802933631
1104	13	0.4704667698332017
1104	14	1.292627487308316
1104	15	0.7234821394991897
1104	16	1.0694778781213543
1104	17	1.6142072816733233
1104	18	2.1381331569911097
1104	19	1.2006649574712966
1104	20	0.6497061141605442
1104	21	0.855580731349967
1104	22	1.2298340287485061
1104	23	2.7287285009432125
1104	24	1.7503932933500934
1104	25	1.7920447588146402
1105	1	0.48608356864586
1105	2	0.9523520671984755
1105	3	0.7111338856014271
1105	4	0.8159786081291784
1105	5	0.8955383591724129
1105	6	0.7080918126757041
1105	7	0.4958671501185421
1105	8	0.5253182083040429
1105	9	0.8118139921360168
1105	10	1.1612133306130161
1105	11	0.6988009603519743
1105	12	0.8067665238220414
1105	13	0.7447292793216675
1105	14	1.5127713689817455
1105	15	0.7540672851480679
1105	16	1.177692913219353
1105	17	1.7907306184515832
1105	18	1.7073636853178158
1105	19	1.2791581667872194
1105	20	1.1267751215887643
1105	21	0.7761790154476671
1105	22	1.4199502167789433
1105	23	2.9490467168581844
1105	24	1.9440182376123474
1105	25	1.9270647647500567
1106	1	-0.4522477718131377
1106	2	-0.12398353892436376
1106	3	-0.25145149613289064
1106	4	-2.6241718767021602
1106	5	-0.4763937553128912
1106	6	-0.511958632906655
1106	7	-0.4758638513996942
1106	8	-0.3716060715989613
1106	9	-0.2583111482018907
1106	10	-0.18359713334638172
1106	11	-0.2731561030944505
1106	12	-0.2089857025684836
1106	13	-0.23021679136908801
1106	14	0.41405958729124137
1106	15	-0.2449937819981045
1106	16	-1.2445977665181331
1106	17	-6.261666459477407
1106	18	-7.265200368097304
1106	19	-3.1796218898651816
1106	20	-1.5465291857580965
1106	21	-1.1853283886129233
1106	22	-1.5623043782416062
1106	23	-5.180189205994761
1106	24	-2.1439797908112155
1106	25	-0.7460925871306969
1107	1	0.29117860874089985
1107	2	0.6635014583478664
1107	3	0.43924347871102043
1107	4	0.675631117781684
1107	5	0.6567634853975406
1107	6	0.23170779129168295
1107	7	0.14727641652780932
1107	8	0.12096863895447285
1107	9	0.1313833417053658
1107	10	0.46253943693912447
1107	11	0.15201301856403404
1107	12	0.25213480419031953
1107	13	0.18459711418950064
1107	14	1.052204098414478
1107	15	0.5420862931670811
1107	16	-6.236273525747084
1107	17	-1.215735078014113
1107	18	0.4709707539248811
1107	19	0.47269247032151895
1107	20	0.25112766094130734
1107	21	0.43244423671288956
1107	22	0.5042225660512969
1107	23	2.424830797642265
1107	24	1.2489989325930395
1107	25	0.8434755161608081
1108	1	0.2216257148263061
1108	2	0.5780796461175441
1108	3	0.23220062794476704
1108	4	0.7002926264857479
1108	5	0.52741740594508
1108	6	0.09756927023826734
1108	7	0.03539053645043566
1108	8	0.08611296271582347
1108	9	0.2068081296833455
1108	10	0.6309526195012438
1108	11	0.1501836776921408
1108	12	0.32418286251497364
1108	13	0.025453385577684173
1108	14	0.6652810214914027
1108	15	0.39394167183096584
1108	16	0.6473858999336173
1108	17	0.7798623033279526
1108	18	0.5303079061343752
1108	19	0.44469217274675543
1108	20	0.23677191030895983
1108	21	0.4933377049467822
1108	22	0.7089041610520397
1108	23	2.452327449926152
1108	24	1.1322291066742807
1108	25	1.4106879845434932
1109	1	0.07256769366209426
1109	2	0.2854445305661706
1109	3	0.17559139684793124
1109	4	-0.02536276601036036
1109	5	-0.012152063884983022
1109	6	-0.08921552087016238
1109	7	-0.22388566298289447
1109	8	-0.21734352288979863
1109	9	-0.11651597610220676
1109	10	0.35408966041595136
1109	11	0.13110734930973678
1109	12	0.08944602507952482
1109	13	0.025962675233962784
1109	14	-1.3168290702517211
1109	15	-0.3018163179832385
1109	16	-0.5316393220303084
1109	17	0.420888069480597
1109	18	1.2120210950210932
1109	19	0.40174681047678007
1109	20	0.15293076609303569
1109	21	0.1149224771269104
1109	22	-0.05960594488730919
1109	23	-4.900150383256349
1109	24	-1.7304200276516042
1109	25	-0.48167503665029443
1110	1	0.5813053782013782
1110	2	0.881428928414195
1110	3	0.8133944248160567
1110	4	1.2441454888156542
1110	5	0.8474418383995186
1110	6	0.5697468127405614
1110	7	0.4811267338103775
1110	8	0.34717373844625143
1110	9	0.38745219632965444
1110	10	0.8133544655089082
1110	11	0.516317581116077
1110	12	0.6076332680258503
1110	13	0.3150322650781803
1110	14	1.2069756836787846
1110	15	0.7045935432891071
1110	16	1.1347726453270823
1110	17	1.4098735015362394
1110	18	1.9987662861269087
1110	19	1.1695963092970274
1110	20	0.8470878355185008
1110	21	0.7027102659430895
1110	22	0.9454306804452983
1110	23	2.3252854736120554
1110	24	1.3797230833955823
1110	25	1.7275294111273567
1111	1	0.691197882660699
1111	2	0.9951728236317976
1111	3	0.7211987747239093
1111	4	1.2965361290008346
1111	5	1.0696433542198136
1111	6	0.486765325380631
1111	7	0.41179025347619813
1111	8	0.2823882925992578
1111	9	0.40911937530527354
1111	10	0.7178184772705194
1111	11	0.37716720992241193
1111	12	0.5184506088045566
1111	13	0.34290187225865765
1111	14	-0.1966486718519036
1111	15	0.1731155780183542
1111	16	0.7076753264478413
1111	17	1.1039651693824695
1111	18	1.88499209907188
1111	19	0.9057061059005989
1111	20	0.7330052859341123
1111	21	0.610147763552547
1111	22	0.9164753133040406
1111	23	0.8211201365755798
1111	24	0.769664602002166
1111	25	0.4448698548064556
1112	1	0.2281983444440563
1112	2	0.7311637585383011
1112	3	0.4149769313049383
1112	4	0.9737181395371266
1112	5	0.6068693772551157
1112	6	0.14141954615347885
1112	7	0.047797796872892206
1112	8	0.1305892171759524
1112	9	0.1621104465585006
1112	10	0.524230532790007
1112	11	0.07602161686745745
1112	12	0.038303365561432656
1112	13	0.00879095111027084
1112	14	0.3643807964351353
1112	15	0.09729894248325621
1112	16	0.4807023528379446
1112	17	0.7995220054875318
1112	18	0.9575796176792863
1112	19	0.3311603565238386
1112	20	0.23517953929550828
1112	21	0.1938837312019004
1112	22	0.24178878452899255
1112	23	1.9230502563530933
1112	24	0.805765624265959
1112	25	1.071129448874757
1113	1	0.3567735255200297
1113	2	0.8131820701337418
1113	3	0.5291310180722455
1113	4	0.6015083107641459
1113	5	0.7660448619157592
1113	6	0.34152110663131197
1113	7	0.3464138434283939
1113	8	0.28719333531075364
1113	9	0.32828055268152667
1113	10	0.5277846011316569
1113	11	0.2946086101069909
1113	12	0.28482936109540447
1113	13	0.127252309871011
1113	14	0.8812166940060209
1113	15	0.3651789607334308
1113	16	0.6845035772668595
1113	17	1.0713454438032528
1113	18	1.7332132190620868
1113	19	0.8317759161154896
1113	20	0.4857745345355511
1113	21	0.5487037958671905
1113	22	0.7382171679406326
1113	23	0.4994720482045665
1113	24	0.5010603361018156
1113	25	0.8575297221887013
1114	1	-0.4904352185019931
1114	2	-3.3501800589779016
1114	3	-1.2547753766201986
1114	4	-0.022597371010796508
1114	5	0.2600581625208491
1114	6	0.04369093829652115
1114	7	-0.008800951512682786
1114	8	0.02443740807400019
1114	9	0.1041832749608318
1114	10	0.33412823498128574
1114	11	-0.03865971073125962
1114	12	0.08075513902540621
1114	13	0.05440105211707902
1114	14	0.8213909274928426
1114	15	0.3077729275738861
1114	16	0.6385373647362584
1114	17	1.0941759292721684
1114	18	1.210566671384143
1114	19	0.48542343422385414
1114	20	0.35532617031335434
1114	21	0.14403748439073638
1114	22	0.3567190897734136
1114	23	1.915091379432127
1114	24	0.8969888096718535
1114	25	0.8308087938737003
1115	1	0.05795430967548043
1115	2	0.38102841737015325
1115	3	0.2079817964680295
1115	4	0.29888776767385394
1115	5	0.19983681393815544
1115	6	-0.1315524531367842
1115	7	-0.19156973731834626
1115	8	-0.14755400662424734
1115	9	-0.28189487540462466
1115	10	0.07544741680972322
1115	11	-0.014446363175721899
1115	12	-0.2919449945156458
1115	13	-0.320115322906112
1115	14	0.5601767799385584
1115	15	-0.07680658965104215
1115	16	0.37984796958001965
1115	17	0.7808932469319672
1115	18	1.2392345928896589
1115	19	0.3837708312129635
1115	20	-0.07226198679413898
1115	21	0.04517967884086005
1115	22	0.31623910091829543
1115	23	1.970808511517177
1115	24	0.761644511313385
1115	25	0.3903374859667039
1116	1	0.0897797523003625
1116	2	0.0071506955295816965
1116	3	0.07641864637073326
1116	4	0.7100354248327463
1116	5	0.2591562766979365
1116	6	-0.11531748457867508
1116	7	-0.12838006141592473
1116	8	-0.06357607475487725
1116	9	-0.05350697811721972
1116	10	0.3810964946781894
1116	11	3.4804408036848145E-4
1116	12	-0.013423616201372113
1116	13	-0.12363851784085256
1116	14	0.571567926666809
1116	15	-0.08391525651630616
1116	16	0.25333090420990345
1116	17	0.5901288704722774
1116	18	1.0891751251007733
1116	19	0.35392981234733156
1116	20	0.08789002501002585
1116	21	0.07592501262332974
1116	22	0.41783987441156967
1116	23	1.7433210586837617
1116	24	0.8742889638538927
1116	25	1.1520747894127368
1201	1	1.0380016305877895
1201	2	1.4351237580847247
1201	3	1.175952676434541
1201	4	1.7509294471372954
1201	5	1.4717481864562245
1201	6	1.0488826887887797
1201	7	0.9344323433376687
1201	8	0.9769281885536429
1201	9	1.2064479032377093
1201	10	1.4647917470792606
1201	11	1.0565295720185333
1201	12	1.2064175898215552
1201	13	1.1134689702570597
1201	14	2.0619397327085807
1201	15	1.5953339524290584
1201	16	2.0365540245864366
1201	17	2.4031260687945277
1201	18	3.005662939225658
1201	19	2.0883714373370026
1201	20	1.787074971387426
1201	21	1.5969424314378173
1201	22	1.7886766998252384
1201	23	3.5048568058425005
1201	24	2.362308399577927
1201	25	2.3892459708453444
1202	1	0.9455939933562867
1202	2	1.2947937660401756
1202	3	0.9392280940956361
1202	4	1.5340311177816872
1202	5	1.297671177705233
1202	6	0.9994462528301469
1202	7	0.76587641652781
1202	8	0.7864301774160154
1202	9	1.0938448801669054
1202	10	1.441600975400661
1202	11	1.1163360954871067
1202	12	1.2029194195749398
1202	13	1.0903663449587313
1202	14	2.0780810214914
1202	15	1.4054093700901547
1202	16	1.685218781945224
1202	17	2.041249537370504
1202	18	2.6840015231556507
1202	19	1.9800770857061352
1202	20	1.5616353532489953
1202	21	1.5543365444052029
1202	22	1.8839610275897556
1202	23	3.473307720719184
1202	24	2.1657989325930416
1202	25	2.365860131545425
1203	1	0.9278961311235072
1203	2	1.245097170632416
1203	3	0.8763185137314231
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2211	15	-0.516046809685129
2211	16	-0.35208309071395405
2211	17	-0.04915196075683781
2211	18	-5.376146791451088
2211	19	-2.0528592438818762
2211	20	-1.1277069688483792
2211	21	-0.826324129752102
2211	22	-0.39171088252260233
2211	23	-4.802148459056092
2211	24	-2.029540393249654
2211	25	-1.468686684933978
2212	1	0.5521496673852262
2212	2	0.8165403006936423
2212	3	0.6395823972182555
2212	4	1.1123028618791722
2212	5	1.1128525943084284
2212	6	0.6367458720228356
2212	7	0.6340205140144803
2212	8	0.5172786161060401
2212	9	0.33746864254314346
2212	10	-3.4008535562063464
2212	11	-0.779011353103904
2212	12	0.07730997555361085
2212	13	0.2852330623997048
2212	14	1.0684798029080014
2212	15	0.7729773822759896
2212	16	0.9248650881143021
2212	17	1.4874865518411795
2212	18	1.9977529321427028
2212	19	1.2124716020808535
2212	20	0.7736676457090113
2212	21	0.7001860493556933
2212	22	0.9456886742005679
2212	23	2.2470432881220788
2212	24	1.3852289402091884
2212	25	1.1374244879810718
2213	1	-1.4060095914084627
2213	2	-3.938574717903066
2213	3	-1.838474967890921
2213	4	-3.3900522279987477
2213	5	-1.1296181426831176
2213	6	-0.9318881758479733
2213	7	-0.8372008052795152
2213	8	-0.8323061930096785
2213	9	-0.7780490705425791
2213	10	-0.8241707945769363
2213	11	-0.9195366453642748
2213	12	-0.9163752779605403
2213	13	-1.2975500105304434
2213	14	-6.180350046469766
2213	15	-2.791386447684303
2213	16	-1.9735961545745653
2213	17	-0.74972312879828
2213	18	-0.18474619902507783
2213	19	-0.7237302331885616
2213	20	-0.8576960881251594
2213	21	-1.0876192434962206
2213	22	-1.0802173144565472
2213	23	-5.6448518013121785
2213	24	-3.1456164520223417
2213	25	-2.3403444987757105
2214	1	-0.19000717197510397
2214	2	0.0777050551880265
2214	3	-0.1300441564506123
2214	4	0.19190934065131415
2214	5	-0.11032794829622361
2214	6	-0.2928316787066336
2214	7	-0.49475198842485213
2214	8	-0.46769655237277163
2214	9	-0.3524493660093526
2214	10	0.1679092055536131
2214	11	-0.34982209824923416
2214	12	-0.3043269023478601
2214	13	-0.3411267358861352
2214	14	0.15719653496554287
2214	15	-0.10541888919608056
2214	16	0.12719081399183807
2214	17	0.5405308192350375
2214	18	0.9146115741389025
2214	19	0.17615428891079432
2214	20	-0.10281737799645185
2214	21	-0.21729608487375174
2214	22	0.19303342380242583
2214	23	1.585973998942059
2214	24	0.64722908554279
2214	25	0.43777855834805024
2215	1	-2.4178188280852204
2215	2	-4.217621880464716
2215	3	-2.386565314089804
2215	4	-3.8003015153222606
2215	5	-4.808674204402699
2215	6	-2.2256038014979964
2215	7	-1.5973034458409003
2215	8	-1.8874966606861214
2215	9	-1.640647694987024
2215	10	-3.041771570778259
2215	11	-1.966172560796842
2215	12	-1.7182701494218051
2215	13	-1.631140355512116
2215	14	-0.8777106481934993
2215	15	-1.5245907747848904
2215	16	-2.1584707508327554
2215	17	-6.8017231536834615
2215	18	-2.9814554851746635
2215	19	-2.1636396835803566
2215	20	-1.925193766635104
2215	21	-1.8774422314006713
2215	22	-1.7549687804508096
2215	23	-6.565237878701311
2215	24	-3.6635725994605615
2215	25	-3.2862919872521132
2216	1	-0.8496678680852838
2216	2	-0.40842834732581323
2216	3	-0.7137518919085615
2216	4	-2.959393374870519
2216	5	-1.2283361001114237
2216	6	-0.8456655736219219
2216	7	-0.8340846751446946
2216	8	-0.7235544272026004
2216	9	-0.7741929084964951
2216	10	-0.38735101900101654
2216	11	-0.8951751711329052
2216	12	-1.773477781264809
2216	13	-1.0710192393659739
2216	14	-6.767749069481308
2216	15	-3.0137548006586243
2216	16	-1.546513338418663
2216	17	-0.700847313574215
2216	18	-0.06144648244266904
2216	19	-0.5397115776948453
2216	20	-0.9931391743927094
2216	21	-1.2101888579740887
2216	22	-0.7278813796880641
2216	23	0.39839407481296263
2216	24	-0.33307944389401456
2216	25	-0.22377457804169865
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	73986.0
3	1.0
4	622.0
5	3877.0
6	4671.0
7	4255.0
8	3882.0
9	3753.0
10	3612.0
11	3523.0
12	3343.0
13	3457.0
14	3499.0
15	3532.0
16	3830.0
17	3903.0
18	4452.0
19	5110.0
20	6476.0
21	8260.0
22	10332.0
23	12422.0
24	14683.0
25	17169.0
26	19866.0
27	22763.0
28	25256.0
29	30886.0
30	35946.0
31	41332.0
32	53056.0
33	69199.0
34	90744.0
35	119410.0
36	166168.0
37	230685.0
38	577350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.07719817266965	17.428727581638775	12.33745017316866	25.156624072522916
2	14.577794980723862	18.646265344625537	41.71416935693567	25.06177031771493
3	19.801541181065627	22.877730040446803	28.263375521315005	29.05735325717257
4	10.84023403114915	15.407070437867604	38.416591827903886	35.33610370307936
5	12.954073416289392	37.22801004590225	35.8128342277732	14.005082310035158
6	34.11177514406614	35.40305877186214	17.174582638472632	13.310583445599084
7	29.93632538919021	30.56933256497285	20.407686057877488	19.08665598795945
8	26.236543695820163	36.28534190200238	19.027620663090588	18.450493739086877
9	26.333345797698605	15.591971286850747	18.9688052549764	39.105877660474256
10	14.404609551035334	28.98634514885948	33.944153059698124	22.66489224040706
11	35.905514895273726	22.389202380584987	22.261306473016635	19.443976251124653
12	23.213022443156188	24.521118501953744	30.63375684285992	21.632102212030137
13	29.195325899242153	20.783131457600163	25.194150845979003	24.827391797178684
14	21.150699468165605	20.687236152631307	27.69960407686286	30.462460302340226
15	24.31706929132363	28.783330484573966	22.764495437237084	24.13510478686532
16	23.286249280385533	27.77189556836189	26.081617633966992	22.860237517285583
17	22.490524513999265	27.55891538477061	27.189867016762086	22.760693084468038
18	22.144739754856026	27.06744159684837	28.456191182152313	22.331627466143296
19	23.697846799640065	26.827929162106145	27.17759569977231	22.296628338481483
20	23.81244328328711	26.630162222410824	27.338152980368672	22.219241513933394
21	23.892927604635318	26.136927500172124	26.957319664483226	23.01282523070933
22	23.351160761454764	26.566508574379554	27.068624715819716	23.013705948345965
23	21.995226764307688	26.078770853229233	28.768627460290798	23.15737492217228
24	23.004931167268495	26.652404055346373	27.201380038240735	23.141284739144396
25	23.227659890191426	26.20738982044814	27.187060394717317	23.377889894643122
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	122.0
1	122.0
2	239.5
3	357.0
4	357.0
5	357.0
6	740.5
7	1124.0
8	1124.0
9	1124.0
10	1862.0
11	2600.0
12	2600.0
13	2600.0
14	4625.5
15	6651.0
16	6651.0
17	6651.0
18	11401.5
19	16152.0
20	16152.0
21	16152.0
22	25132.0
23	34112.0
24	34112.0
25	34112.0
26	47709.0
27	61306.0
28	61306.0
29	61306.0
30	79097.5
31	96889.0
32	96889.0
33	96889.0
34	115590.5
35	134292.0
36	134292.0
37	134292.0
38	152686.0
39	171080.0
40	171080.0
41	171080.0
42	186847.0
43	202614.0
44	202614.0
45	202614.0
46	217615.0
47	232616.0
48	232616.0
49	232616.0
50	229309.5
51	226003.0
52	226003.0
53	226003.0
54	206192.0
55	186381.0
56	186381.0
57	186381.0
58	162094.5
59	137808.0
60	137808.0
61	137808.0
62	113103.0
63	88398.0
64	88398.0
65	88398.0
66	69074.0
67	49750.0
68	49750.0
69	49750.0
70	36335.0
71	22920.0
72	22920.0
73	22920.0
74	15965.0
75	9010.0
76	9010.0
77	9010.0
78	6261.5
79	3513.0
80	3513.0
81	3513.0
82	2334.0
83	1155.0
84	1155.0
85	1155.0
86	749.0
87	343.0
88	343.0
89	343.0
90	219.5
91	96.0
92	96.0
93	96.0
94	56.0
95	16.0
96	16.0
97	16.0
98	9.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11754507031639858
2	0.050139113789680355
3	0.03732248825290999
4	0.0342963405567281
5	0.0382125316929635
6	0.019046929617144846
7	0.020055645515872147
8	0.02450586271613963
9	0.030142804503145118
10	0.037678505628931394
11	0.020589671579904243
12	0.020352326662556642
13	0.016673480443668856
14	0.023022456982717134
15	0.02177639616664224
16	0.02260710337735884
17	0.02592993222022523
18	0.020292990433219745
19	0.03340629711667461
20	0.023853164193433734
21	0.030083468273808214
22	0.038034523004952796
23	0.029668114668449916
24	0.042722085122567886
25	0.03328762465800081
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1685311.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.53266814339531
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.52695353138515	63.84546330258311
2	10.549487677857167	15.936619037088468
3	2.590873820146361	5.87086837375578
4	0.9746515420498725	2.944721259244061
5	0.46092122211363096	1.7407304855078545
6	0.25068902504161183	1.1361126561395631
7	0.14770835072226965	0.7809764085979408
8	0.10843376966172369	0.6552233551517074
9	0.07537188826005103	0.5123735840958757
>10	0.29469932589760245	3.649621246328046
>50	0.012902154740581732	0.6688345991695289
>100	0.005719294556007277	0.9138257216333672
>500	0.0011118782975267304	0.5937607606529142
>1k	4.7651927036859867E-4	0.7508692100517809
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3119	0.1850696993017906	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2656	0.15759702511880597	No Hit
CCCATGTACTCTGCGTTGATACCAC	2433	0.1443650459766773	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	5.9336229336899835E-5	0.0	0.0	0.0	0.0
7	5.9336229336899835E-5	0.0	0.0	0.0	0.0
8	5.9336229336899835E-5	0.0	0.0	0.0	0.0
9	5.9336229336899835E-5	0.0	0.0	0.0	0.0
10	5.9336229336899835E-5	0.0	0.0	0.0	0.0
11	5.9336229336899835E-5	0.0	0.0	0.0	0.0
12	5.9336229336899835E-5	0.0	0.0	0.0	0.0
13	5.9336229336899835E-5	0.0	0.0	5.9336229336899835E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACAC	220	0.0	12.523157	3
GCGATGC	75	2.0762102E-4	11.3987	13
GTCCTAC	445	0.0	11.11371	1
TGGACCG	60	0.005873205	11.084043	5
CCGTGCG	60	0.0058809957	11.082069	9
GCGCCAC	60	0.0058809957	11.082069	13
TAGGACC	250	0.0	11.020706	4
CTAGTAC	105	3.4647437E-6	10.857517	3
GACTATC	70	0.0014925654	10.856227	7
CTATACT	260	0.0	10.596833	4
GTGTTAG	225	0.0	10.567524	1
CTAAGAC	180	3.45608E-11	10.555919	3
GCGTTTA	90	9.526253E-5	10.554352	13
ATAGGAC	190	9.094947E-12	10.500361	3
TAGCCCT	470	0.0	10.309171	4
GTCCTAG	240	0.0	10.303335	1
TAGGACT	260	0.0	10.231425	4
TTAGACA	140	9.6770236E-8	10.179223	4
GACTGCG	75	0.0026496225	10.132479	7
TATACTG	340	0.0	10.059467	5
>>END_MODULE