FastQCFastQC Report
Thu 26 May 2016
SRR1513084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513084_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685311
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40620.24102376356648714No Hit
GTACATGGGAAGCAGTGGTATCAAC36030.2137884343008501No Hit
CCCATGTACTCTGCGTTGATACCAC31990.18981659764874256No Hit
TATCAACGCAGAGTACTTTTTTTTT28720.17041365065557632No Hit
GGTATCAACGCAGAGTACTTTTTTT26270.15587627446803587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC802.0362586E-613.04142910
GCGTGCG854.0050454E-612.2772019
TTACACT2550.011.5757264
ATTACAC2100.011.3356763
GTATTAG1604.3655746E-1111.317451
GACCGAG855.396793E-511.1614237
GTCCTAC4200.010.8919821
TAGGACC3350.010.8010384
CTAGGAC2400.010.7122143
GGCGAGA2350.010.4952419
GTGTAGG2500.010.2929231
TCTAGGA3150.010.2844192
GTATTAC1605.853508E-910.1261391
GACACCG750.002677526310.1196897
GGGCCGT951.6061266E-410.0231246
TACACTG6750.010.01575
TGGACCG1054.0018793E-59.9753955
GTACTAG1159.879512E-69.9448531
TGTACTG4650.09.8291275
GAACCAT1951.5643309E-109.7661216
Produced by FastQC (version 0.11.5)