FastQCFastQC Report
Thu 26 May 2016
SRR1513083_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513083_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827028
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC125860.6888783313665691No Hit
CCCATGTACTCTGCGTTGATACCAC101140.5535766282728015No Hit
GAGTACATGGGAAGCAGTGGTATCA55090.3015279459318631No Hit
GCGTTGATACCACTGCTTCCCATGT34970.1914037442228581No Hit
GTATCAACGCAGAGTACATGGGAAG33690.1843978307940546No Hit
CATGTACTCTGCGTTGATACCACTG32840.17974546640773978No Hit
ACGCAGAGTACATGGGAAGCAGTGG26050.1425812850158837No Hit
GCTTCCCATGTACTCTGCGTTGATA25410.13907832830148198No Hit
TATCAACGCAGAGTACATGGGAAGC24660.1349733009017924No Hit
ACTCTGCGTTGATACCACTGCTTCC21920.11997626746825993No Hit
GTATCAACGCAGAGTACTTTTTTTT19230.10525290252804008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTTA1900.013.998548513
TGCGTTT2100.013.11696812
GTACCGG802.002278E-613.0611466
CTCGAAC1155.326001E-912.39137518
TGGACCG853.9405077E-612.2951985
TAGGACG2950.012.2390344
TACGACT550.003065521112.0926354
TATCGAC550.003069295512.09064819
CGCTCAA550.00307181412.08932510
AGGACGT2800.011.8760435
TCGAACT1201.0018994E-811.874742519
GTAGGAC5200.011.6930153
ACCGAGC658.033434E-411.6907758
GTCCTAA2400.011.4902441
TGTAGGA6200.011.3397062
CGTTTAT2450.011.24373114
TAGGACC3550.011.241044
GGACGTG2900.011.1367756
CCGTACA600.00587248411.0843082
TGGCGAG2400.011.08339718
Produced by FastQC (version 0.11.5)