##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513083_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1827028
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.388948062098667	31.0	30.0	34.0	25.0	34.0
2	29.117166786715913	31.0	30.0	34.0	18.0	34.0
3	29.46627309488415	31.0	30.0	34.0	24.0	34.0
4	32.32308207646517	35.0	33.0	37.0	19.0	37.0
5	32.56300012917153	35.0	33.0	37.0	24.0	37.0
6	33.05688692236791	37.0	35.0	37.0	27.0	37.0
7	33.07552757812141	37.0	35.0	37.0	27.0	37.0
8	33.06153162403641	37.0	35.0	37.0	27.0	37.0
9	34.464001646389654	39.0	35.0	39.0	27.0	39.0
10	33.96459933837905	39.0	34.0	39.0	23.0	39.0
11	34.34065597243173	39.0	35.0	39.0	27.0	39.0
12	34.1703920246433	39.0	35.0	39.0	25.0	39.0
13	34.263647300424516	39.0	35.0	39.0	26.0	39.0
14	34.5928179535289	40.0	34.0	41.0	17.0	41.0
15	35.133583064955765	40.0	34.0	41.0	25.0	41.0
16	34.7119863516049	40.0	34.0	41.0	18.0	41.0
17	34.24878272254175	39.0	33.0	41.0	17.0	41.0
18	33.646384182398954	39.0	32.0	41.0	11.0	41.0
19	34.54058941625416	39.0	34.0	41.0	18.0	41.0
20	34.80695862351316	39.0	34.0	41.0	21.0	41.0
21	34.838004672068514	40.0	35.0	41.0	19.0	41.0
22	34.43389099674444	40.0	34.0	41.0	16.0	41.0
23	32.74637936583348	39.0	31.0	41.0	8.0	41.0
24	33.857707161576066	39.0	33.0	41.0	2.0	41.0
25	33.59491808554658	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6495322177175673
1101	2	1.1108625445168165
1101	3	0.7792360730203143
1101	4	0.7857088370536331
1101	5	0.9012404696238008
1101	6	0.6513323271962648
1101	7	0.6852012781457049
1101	8	0.6628993025108798
1101	9	0.9325434471804144
1101	10	1.1431846156218413
1101	11	0.7871652609156428
1101	12	0.8888142238299963
1101	13	0.7032935681796175
1101	14	-0.21176786778527656
1101	15	0.3388521543986798
1101	16	1.0534435070376134
1101	17	1.7054221021467484
1101	18	-4.482571275730301
1101	19	-0.7949348273063066
1101	20	0.34459866890333046
1101	21	0.6763174753223282
1101	22	1.195659893293822
1101	23	2.9824676954721454
1101	24	1.9815054173570843
1101	25	2.105925231266191
1102	1	1.0399000670646608
1102	2	1.2937106645844985
1102	3	1.1390593813395462
1102	4	1.6787438524883243
1102	5	1.5326975301871997
1102	6	1.1432727066700679
1102	7	1.02579774172888
1102	8	0.8613461464719734
1102	9	1.0321869639648327
1102	10	1.4419433823965733
1102	11	1.0687049842536638
1102	12	1.2235061112536343
1102	13	1.19136235910738
1102	14	2.0292716166056692
1102	15	1.4962143077639638
1102	16	1.7278806897869217
1102	17	1.464386492573368
1102	18	1.9393563168455472
1102	19	1.6863136389117415
1102	20	1.4182886576663662
1102	21	1.4082014116203254
1102	22	1.8310169577320394
1102	23	3.4496395952951957
1102	24	2.284474173411489
1102	25	2.6866951575154943
1103	1	0.7288728554158901
1103	2	1.1015758580817163
1103	3	0.7888170035018973
1103	4	1.2920942328451375
1103	5	1.213512181045516
1103	6	0.8903454879655541
1103	7	0.8427357060686305
1103	8	0.7706240565086375
1103	9	0.9408461898714293
1103	10	1.074719660466016
1103	11	0.9562007704071647
1103	12	1.0184321174156494
1103	13	0.9168496626898701
1103	14	1.7298865115404283
1103	15	1.273391723114642
1103	16	1.4926684817836176
1103	17	1.8697862265457275
1103	18	1.971891393034504
1103	19	1.4087603092543617
1103	20	1.3680248512759476
1103	21	1.3064950977756524
1103	22	1.6784324652098164
1103	23	3.0790613902239414
1103	24	2.0146402185630166
1103	25	2.1944317796961386
1104	1	0.7794017039862204
1104	2	1.1800467312518492
1104	3	0.985736276627879
1104	4	1.5505369743389181
1104	5	1.3084043826795266
1104	6	0.9199991714326359
1104	7	0.8643521193394861
1104	8	0.9143285251909674
1104	9	1.0000776442879058
1104	10	1.4042090049912233
1104	11	0.9303230222056129
1104	12	1.000909409727548
1104	13	0.8600843287499274
1104	14	1.7172830904508416
1104	15	1.1982052969982533
1104	16	1.5177964319435517
1104	17	2.10602844292179
1104	18	2.607070989615181
1104	19	1.6773516216496631
1104	20	1.180097584276858
1104	21	1.236937338713176
1104	22	1.548187700453127
1104	23	3.068700238364187
1104	24	2.113402015445992
1104	25	2.2380744820159535
1105	1	0.5376081935751564
1105	2	0.9838810398132267
1105	3	0.7296086092151377
1105	4	0.8278039304281037
1105	5	0.8357334554732176
1105	6	0.6004487040771735
1105	7	0.33038199911899113
1105	8	0.3826441984879736
1105	9	0.6570340320358099
1105	10	1.1542751700349214
1105	11	0.6666874567361276
1105	12	0.6326895555588479
1105	13	0.6281419457254174
1105	14	1.4493848768311182
1105	15	0.6624512508097382
1105	16	1.103191466351909
1105	17	1.5991919366182756
1105	18	1.7084470960136287
1105	19	1.2872499880941888
1105	20	1.0441958496141197
1105	21	0.8593845445747661
1105	22	1.3576357903707859
1105	23	2.8273000494079596
1105	24	1.73853934270209
1105	25	1.827431195857578
1106	1	-0.5902474937188309
1106	2	-0.1129111912155345
1106	3	-0.3741291918894625
1106	4	-2.6191320074077
1106	5	-0.5096881233337811
1106	6	-0.5156938427181643
1106	7	-0.540082109134957
1106	8	-0.46916637781516357
1106	9	-0.3535584542990975
1106	10	-0.23968241185853856
1106	11	-0.3591398931290968
1106	12	-0.2611890523235374
1106	13	-0.2501804650274764
1106	14	0.4096948692444329
1106	15	-0.26289927728041107
1106	16	-1.2336301015799833
1106	17	-6.226224230526984
1106	18	-7.160079171260318
1106	19	-3.0289360591400367
1106	20	-1.5576920176473976
1106	21	-1.2004211793579884
1106	22	-1.4592182993177971
1106	23	-4.85123999300497
1106	24	-2.10780908027753
1106	25	-0.7612778509390523
1107	1	0.3707642832547329
1107	2	0.7769296356261535
1107	3	0.47405928057981583
1107	4	0.7156632245537011
1107	5	0.6821231997355923
1107	6	0.3925653733770389
1107	7	0.3043238284225467
1107	8	0.41174817778809825
1107	9	0.3077088993576922
1107	10	0.7472458631010852
1107	11	0.4441463118638467
1107	12	0.4622396622279794
1107	13	0.3760514145856888
1107	14	1.1815533206541957
1107	15	0.6518674323231366
1107	16	-5.976277160403512
1107	17	-1.039983456845249
1107	18	0.5963455154026676
1107	19	0.4130608126013584
1107	20	0.2871105749224725
1107	21	0.2615939916739265
1107	22	0.43033934855887424
1107	23	2.259751337833933
1107	24	1.2751347541905602
1107	25	1.0042888136829973
1108	1	0.3259797563678788
1108	2	0.5689941034282775
1108	3	0.3599203774301323
1108	4	1.0122952750159016
1108	5	0.6083280868351295
1108	6	0.22222767774143648
1108	7	0.2161756002137949
1108	8	0.24728292759370163
1108	9	0.40820434941338846
1108	10	0.8553896029121191
1108	11	0.304169474397888
1108	12	0.44892796026959303
1108	13	0.19685288629977293
1108	14	0.8740301506144732
1108	15	0.5671811998451517
1108	16	0.7765521063514242
1108	17	0.895211735915673
1108	18	0.6086454514542581
1108	19	0.6102677232327096
1108	20	0.5827541270914622
1108	21	0.6604601833810548
1108	22	0.9776465350990833
1108	23	2.657899611076779
1108	24	1.378108205092893
1108	25	1.607206722539999
1109	1	0.4205454053523816
1109	2	0.6493652540736008
1109	3	0.5951637706198909
1109	4	0.5770057750538555
1109	5	0.38947591733913356
1109	6	0.2882812688164478
1109	7	0.33818618500050945
1109	8	0.08750407787782422
1109	9	0.3615391757037756
1109	10	0.8438814133836274
1109	11	0.4680354524792989
1109	12	0.41878391526086745
1109	13	0.38089114757004694
1109	14	-0.7187598553434142
1109	15	0.09132673857135387
1109	16	-0.18130658260162136
1109	17	0.8464427347736461
1109	18	1.6403378374112876
1109	19	0.9094564442843307
1109	20	0.6472465768223472
1109	21	0.5348013590479823
1109	22	0.4151029773165149
1109	23	-4.467840071202186
1109	24	-1.47678524048343
1109	25	-0.0895606452208213
1110	1	0.5132138221801767
1110	2	0.8288794141467406
1110	3	0.7292290814626199
1110	4	1.1314669591212407
1110	5	0.8314903179319657
1110	6	0.5253186137552461
1110	7	0.4862379014231024
1110	8	0.4796529474845741
1110	9	0.6034184905655309
1110	10	0.9585536824736138
1110	11	0.541551963971699
1110	12	0.6700830916720761
1110	13	0.39281771776899177
1110	14	1.3563706050088271
1110	15	0.8694865383800234
1110	16	1.226731511771895
1110	17	1.5251153225830762
1110	18	2.040916737493731
1110	19	1.214645354624011
1110	20	0.9021026078969996
1110	21	0.8414775445387477
1110	22	1.1372711477041193
1110	23	2.4911500864849927
1110	24	1.5453415851844312
1110	25	1.7837989487523274
1111	1	0.4846731359482348
1111	2	0.857074093130116
1111	3	0.6573441047990052
1111	4	1.2253836107898124
1111	5	0.9458200632036906
1111	6	0.2906955009076455
1111	7	0.23550683528416982
1111	8	0.12178018501024468
1111	9	0.24089809760413772
1111	10	0.5240671776052608
1111	11	0.22345439017016133
1111	12	0.4593504877231567
1111	13	0.35162551237966966
1111	14	-0.2146531738026738
1111	15	0.1555751065333908
1111	16	0.6999241362993303
1111	17	1.1244299541417462
1111	18	1.7780765091604778
1111	19	0.7727386739796032
1111	20	0.6982526738966968
1111	21	0.661231174764815
1111	22	0.9166228225344426
1111	23	0.7647135075640534
1111	24	0.8141696914999841
1111	25	0.45993204062846615
1112	1	0.6039709949058647
1112	2	0.9067616410173152
1112	3	0.6256688355666924
1112	4	1.2849253573362986
1112	5	1.00910995710705
1112	6	0.43541946627586015
1112	7	0.34253399669310625
1112	8	0.43768807327619186
1112	9	0.4706029370280689
1112	10	0.8711948377825536
1112	11	0.5231841662036487
1112	12	0.5505568937178751
1112	13	0.2565757121028227
1112	14	0.7397914275111788
1112	15	0.4552425500854156
1112	16	0.982235781586283
1112	17	1.1252998358703863
1112	18	1.179472250369038
1112	19	0.7682840114557408
1112	20	0.5649455595903845
1112	21	0.7865951777688309
1112	22	0.7092717130705282
1112	23	2.292616938273902
1112	24	1.0728058447491478
1112	25	1.0891701218924226
1113	1	0.3211695968953414
1113	2	0.6964660360449066
1113	3	0.4605742906242405
1113	4	0.5861863833121532
1113	5	0.6195323047917611
1113	6	0.20116820845046135
1113	7	0.12636213869615176
1113	8	0.0823463160332949
1113	9	0.07519255948841419
1113	10	0.5319634875245924
1113	11	0.19282992672393107
1113	12	0.21291942908421646
1113	13	0.11212998742896474
1113	14	0.7308848714563325
1113	15	0.2741144119230583
1113	16	0.6606459926961108
1113	17	1.0814903852168314
1113	18	1.6516131780232683
1113	19	0.7585273830679284
1113	20	0.5042780479722992
1113	21	0.4569297017155236
1113	22	0.6468963754100159
1113	23	0.4843863433512965
1113	24	0.6295302497625315
1113	25	0.9153546541606019
1114	1	-0.482007654888303
1114	2	-2.9827397997890763
1114	3	-1.1970032980886316
1114	4	0.037234137079146024
1114	5	0.23844314443679338
1114	6	-0.04712437063749775
1114	7	-0.25536517294278127
1114	8	-0.06946708193918028
1114	9	-0.023636657219796575
1114	10	0.1261043065464449
1114	11	-0.06476047994873113
1114	12	0.01698409569137027
1114	13	-0.10999701566336739
1114	14	0.7061047795790287
1114	15	0.114699506009174
1114	16	0.380577920173927
1114	17	0.8010986592098774
1114	18	0.9874819495820475
1114	19	0.2891776427460613
1114	20	0.16242786703306678
1114	21	0.011867248495356364
1114	22	0.28186354083265286
1114	23	1.870173514862465
1114	24	0.8684705249366615
1114	25	0.6741888607174502
1115	1	0.17884346140765928
1115	2	0.470036561250712
1115	3	0.23931344964089973
1115	4	0.3040942362051382
1115	5	0.3671769040716413
1115	6	-0.13226923836656823
1115	7	-0.2714341258989208
1115	8	-0.26812803434194876
1115	9	-0.24658918261047091
1115	10	0.057426575623949816
1115	11	-0.06778447630870943
1115	12	-0.27156332378307724
1115	13	-0.31746360291784725
1115	14	0.5605662241307456
1115	15	-0.05242384811371181
1115	16	0.47086279567803047
1115	17	0.9049012363779241
1115	18	1.2120433802770592
1115	19	0.5845353984713881
1115	20	-0.016661776436308173
1115	21	0.001838652471846558
1115	22	0.39672902633285645
1115	23	2.0925061939884912
1115	24	0.9399289176438543
1115	25	0.5897854503951621
1116	1	0.4664729488865902
1116	2	0.3078173410267837
1116	3	0.439132698482009
1116	4	1.0884950324360858
1116	5	0.5225797645271157
1116	6	0.40923293212027545
1116	7	0.26611529660218025
1116	8	0.17422476077798166
1116	9	0.19444238255989177
1116	10	0.7110172952408149
1116	11	0.1842001040813699
1116	12	0.35713118571635505
1116	13	0.3223961308161236
1116	14	0.9938456898311614
1116	15	0.3451764119659515
1116	16	0.7437584334518661
1116	17	1.068673254246832
1116	18	1.581738207511357
1116	19	0.7637205032292016
1116	20	0.4783789293980192
1116	21	0.39693806560923406
1116	22	0.8667499000406096
1116	23	2.332662094493287
1116	24	1.1003038027016885
1116	25	1.387336682512057
1201	1	1.0295910119157412
1201	2	1.3193890051607688
1201	3	1.082378750499764
1201	4	1.654977021138322
1201	5	1.3799327804206598
1201	6	0.9643885188788914
1201	7	0.9266204628123447
1201	8	0.9362437628241409
1201	9	1.1134167154790688
1201	10	1.5275089835776967
1201	11	1.158330716590207
1201	12	1.1623438155644408
1201	13	1.0535795324318116
1201	14	2.049756693373844
1201	15	1.489439770903239
1201	16	1.7906628643373494
1201	17	2.2183203723006244
1201	18	2.692257230342449
1201	19	1.7822595073076712
1201	20	1.5018264104952834
1201	21	1.3997553475868543
1201	22	1.6763633926942916
1201	23	3.2544707992225526
1201	24	2.2084695094124456
1201	25	2.3209789415107807
1202	1	0.8617548808066644
1202	2	1.1237483412265554
1202	3	0.8343986023041303
1202	4	1.5626895624411503
1202	5	1.2112606752947457
1202	6	0.8868941771085943
1202	7	0.6367622742604055
1202	8	0.7590579988718957
1202	9	0.7944242638404688
1202	10	1.32032706494725
1202	11	1.0398464080949026
1202	12	0.9445449475806313
1202	13	0.9478408524614892
1202	14	1.7470111230311645
1202	15	1.2357209956303024
1202	16	1.406778359397407
1202	17	1.7496674144441826
1202	18	2.524447281273133
1202	19	1.6559361903235654
1202	20	1.3735484323411526
1202	21	1.2794105274786247
1202	22	1.7490211976661314
1202	23	3.274278702390262
1202	24	1.966763265551343
1202	25	2.2034625750912795
1203	1	0.865022547376114
1203	2	1.2682837519267132
1203	3	1.1562239162811494
1203	4	1.646951107514603
1203	5	1.4497163987049149
1203	6	1.0162395334999346
1203	7	0.9667357706211348
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2211	19	-1.8960518466935241
2211	20	-1.046403358245378
2211	21	-0.7775655457960369
2211	22	-0.4232983404632833
2211	23	-4.794959352412313
2211	24	-2.281479769439091
2211	25	-1.4896928122891566
2212	1	0.27214084786659143
2212	2	0.4469581365370061
2212	3	0.3188752489251989
2212	4	0.7946038917296576
2212	5	0.6855903208497764
2212	6	0.2646491719314241
2212	7	0.25412696646098
2212	8	0.17785266153963875
2212	9	-0.08813300383715017
2212	10	-3.4614449558838523
2212	11	-1.0073125234185198
2212	12	-0.2568681907034005
2212	13	-0.11295418765521248
2212	14	0.7402088807803437
2212	15	0.3343060721147211
2212	16	0.44504122709134464
2212	17	0.9408014445278852
2212	18	1.632330608740702
2212	19	0.8508480253885722
2212	20	0.36876911246336164
2212	21	0.21178595657177368
2212	22	0.5175516460652858
2212	23	1.8702060791710267
2212	24	0.9808770511093314
2212	25	0.7513427401486865
2213	1	-1.553722177996434
2213	2	-3.8138378307807024
2213	3	-1.6809360508861033
2213	4	-3.70131806071403
2213	5	-1.4781191580770958
2213	6	-1.0181742848050774
2213	7	-0.963244068301357
2213	8	-0.7429731310252805
2213	9	-0.686643189303858
2213	10	-0.7893260120257821
2213	11	-0.83546335809109
2213	12	-0.6412140380927269
2213	13	-0.9831060165026955
2213	14	-6.285690823380232
2213	15	-2.7663063589324963
2213	16	-1.9023671616213917
2213	17	-0.6493793891312052
2213	18	0.03065619619672333
2213	19	-0.5808142736650268
2213	20	-0.6821075471089486
2213	21	-0.8401090083666674
2213	22	-0.8238065126841789
2213	23	-5.851029931195416
2213	24	-3.1714003816028864
2213	25	-2.223616841332632
2214	1	-0.04362215625257804
2214	2	0.06785573209213425
2214	3	0.002408154375910243
2214	4	0.35789778961260055
2214	5	-0.05977561458465175
2214	6	-0.23018989311312055
2214	7	-0.28711740560530785
2214	8	-0.3471760768452583
2214	9	-0.33877243290123715
2214	10	0.0655020696933093
2214	11	-0.3276880383145837
2214	12	-0.34097107412135585
2214	13	-0.39103637943603076
2214	14	0.28055278345301105
2214	15	-0.10245032217591898
2214	16	0.2893151038994688
2214	17	0.47978266878308773
2214	18	1.0311667041521417
2214	19	0.12167138011604095
2214	20	-0.0605967271764456
2214	21	-0.2295757638995326
2214	22	0.3357622026320115
2214	23	1.8348913938563456
2214	24	0.6192332154928906
2214	25	0.5635996613097163
2215	1	-2.331373729007826
2215	2	-4.510213750555359
2215	3	-2.4341469442560424
2215	4	-3.9186318453629347
2215	5	-4.969600909391158
2215	6	-2.287200568785117
2215	7	-1.6633045748274178
2215	8	-1.9400137993685682
2215	9	-1.7094771997340317
2215	10	-3.221494217424169
2215	11	-2.1636989137015306
2215	12	-1.7270454951749414
2215	13	-1.736777911975956
2215	14	-0.9262751984273976
2215	15	-1.5769703254439875
2215	16	-2.2783617119179524
2215	17	-6.662476788173805
2215	18	-3.0480702031710436
2215	19	-2.135019751632285
2215	20	-1.9072206366874198
2215	21	-1.8829602514945094
2215	22	-1.5987194916770235
2215	23	-6.393670567707606
2215	24	-3.7673999535295053
2215	25	-3.462657408145695
2216	1	-0.6114968736352075
2216	2	-0.2093265173204628
2216	3	-0.4517790159038242
2216	4	-2.7022775439867246
2216	5	-0.9581408670561729
2216	6	-0.7370979482623028
2216	7	-0.7670149983702998
2216	8	-0.6139990142926166
2216	9	-0.5440091665094258
2216	10	-0.3592463454328154
2216	11	-0.8469791674540943
2216	12	-1.5558622387265757
2216	13	-0.8904622782700997
2216	14	-6.58959173931154
2216	15	-2.988485699220739
2216	16	-1.3617854413858055
2216	17	-0.4117886837903981
2216	18	0.42148013235624404
2216	19	-0.2943247629880972
2216	20	-0.6092357305900222
2216	21	-0.8786591969801734
2216	22	-0.3551009440630324
2216	23	0.6261535297786764
2216	24	-0.12252100714309933
2216	25	0.06451290331884962
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79793.0
3	4.0
4	645.0
5	4119.0
6	5007.0
7	4518.0
8	4364.0
9	4007.0
10	3750.0
11	3795.0
12	3700.0
13	3673.0
14	3738.0
15	3697.0
16	3942.0
17	4255.0
18	4711.0
19	5428.0
20	6842.0
21	8474.0
22	10716.0
23	13058.0
24	15317.0
25	18203.0
26	20622.0
27	24133.0
28	26544.0
29	31942.0
30	38221.0
31	43363.0
32	56542.0
33	74060.0
34	96771.0
35	128744.0
36	181741.0
37	255634.0
38	632955.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.70250881721396	17.14415959910647	11.65159827584223	25.50173330783733
2	14.550936436645278	18.996231473602247	40.571948627132734	25.880883462619746
3	20.364004897054542	23.125058858828606	27.80031143164382	28.710624812473036
4	11.168191653380521	15.35869308127617	37.49682446519614	35.97629080014717
5	13.10634374325859	37.33572857613729	35.56158077264649	13.99634690795763
6	34.762103405311066	34.331266190610705	17.153561989099327	13.753068414978904
7	30.026063635562384	30.339527985972385	20.346606389833376	19.287801988631852
8	27.03061365824977	35.446290190041296	19.300417222571078	18.22267892913786
9	27.077696469457184	15.034782027880372	18.710300144865805	39.17722135779664
10	14.695414879439506	28.773818896991106	33.83446872676422	22.696297496805165
11	35.944123245502865	22.445300528887923	21.601357229001735	20.009218996607476
12	23.75429741388749	24.58306873672455	30.22116626885935	21.44146758052861
13	29.542897839711795	20.4122957366291	24.537169077053363	25.507637346605744
14	21.420261847605023	20.767038844651502	27.337645451882835	30.47505385586064
15	25.70834665463735	27.63887733159317	22.23345713021885	24.419318883550634
16	23.225734399267722	27.283646542346585	26.58120505039611	22.909414007989582
17	23.360721704006316	27.03225615718238	27.073152571546828	22.53386956726447
18	22.952064427231683	26.342037827075444	28.331547763402586	22.374349982290283
19	24.13968874833555	26.737266977363518	27.048146331207512	22.07489794309342
20	24.211887479052738	26.27247947154594	26.838007654612834	22.677625394788492
21	23.626378439460353	26.006201951030427	27.143664297500937	23.223755312008286
22	23.610391715187106	26.26247140515271	25.969706161594146	24.15743071806603
23	21.661838689914685	26.156483386047796	28.347058073141795	23.834619850895727
24	23.225233818336527	27.286480780108096	26.215688346617856	23.27259705493752
25	23.427952905930123	25.726427779894788	26.23538712884731	24.610232185327778
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	143.0
1	143.0
2	265.0
3	387.0
4	387.0
5	387.0
6	717.5
7	1048.0
8	1048.0
9	1048.0
10	1690.5
11	2333.0
12	2333.0
13	2333.0
14	3897.5
15	5462.0
16	5462.0
17	5462.0
18	10014.5
19	14567.0
20	14567.0
21	14567.0
22	22992.5
23	31418.0
24	31418.0
25	31418.0
26	44965.5
27	58513.0
28	58513.0
29	58513.0
30	76154.5
31	93796.0
32	93796.0
33	93796.0
34	113468.0
35	133140.0
36	133140.0
37	133140.0
38	153673.5
39	174207.0
40	174207.0
41	174207.0
42	195377.5
43	216548.0
44	216548.0
45	216548.0
46	247951.0
47	279354.0
48	279354.0
49	279354.0
50	270229.0
51	261104.0
52	261104.0
53	261104.0
54	233333.5
55	205563.0
56	205563.0
57	205563.0
58	178414.5
59	151266.0
60	151266.0
61	151266.0
62	125165.5
63	99065.0
64	99065.0
65	99065.0
66	77271.0
67	55477.0
68	55477.0
69	55477.0
70	40842.5
71	26208.0
72	26208.0
73	26208.0
74	18529.0
75	10850.0
76	10850.0
77	10850.0
78	7609.5
79	4369.0
80	4369.0
81	4369.0
82	2953.5
83	1538.0
84	1538.0
85	1538.0
86	1021.0
87	504.0
88	504.0
89	504.0
90	317.5
91	131.0
92	131.0
93	131.0
94	79.5
95	28.0
96	28.0
97	28.0
98	18.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10426769595211459
2	0.04652364386314824
3	0.034153827965417057
4	0.030869806045665422
5	0.03448223015739223
6	0.019266261929209626
7	0.01888312603857193
8	0.02326182193157412
9	0.02791418631788894
10	0.035139034541342554
11	0.019977800011822477
12	0.019047327134559516
13	0.015380169324170183
14	0.021510343574373245
15	0.02079880549176039
16	0.021838745766348408
17	0.025286968782087627
18	0.018992593435896987
19	0.03262128440286629
20	0.022823952342273898
21	0.027695251523238835
22	0.035576904130642774
23	0.028516257003176743
24	0.0392440619410321
25	0.0304319364563652
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1827028.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.81420543054033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.2935960976696	61.37771222734707
2	10.691023472342538	15.569167587557562
3	2.688147643117689	5.872060041407826
4	1.0108913551455492	2.944290032061012
5	0.4484043391861672	1.6325102834723628
6	0.2449992196441659	1.070365410569542
7	0.14488292659201643	0.7384674626174285
8	0.09624394606163696	0.5606341167982299
9	0.07571559640783314	0.49618538920222593
>10	0.2790656518662777	3.4604998087040326
>50	0.014909493250499926	0.7393511457252145
>100	0.008919474169530933	1.2618361227792605
>500	0.001752810584998653	0.9600513707837779
>1k	0.001219346493912106	1.7515208061626006
>5k	7.620915586950662E-5	0.3057004220361731
>10k+	1.5241831173901324E-4	1.2596477727756632
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	12586	0.6888783313665691	No Hit
CCCATGTACTCTGCGTTGATACCAC	10114	0.5535766282728015	No Hit
GAGTACATGGGAAGCAGTGGTATCA	5509	0.3015279459318631	No Hit
GCGTTGATACCACTGCTTCCCATGT	3497	0.1914037442228581	No Hit
GTATCAACGCAGAGTACATGGGAAG	3369	0.1843978307940546	No Hit
CATGTACTCTGCGTTGATACCACTG	3284	0.17974546640773978	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2605	0.1425812850158837	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2541	0.13907832830148198	No Hit
TATCAACGCAGAGTACATGGGAAGC	2466	0.1349733009017924	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2192	0.11997626746825993	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1923	0.10525290252804008	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	5.473369866252734E-5	0.0	0.0
8	0.0	0.0	5.473369866252734E-5	5.473369866252734E-5	0.0
9	0.0	0.0	5.473369866252734E-5	1.0946739732505468E-4	0.0
10	0.0	0.0	5.473369866252734E-5	1.0946739732505468E-4	0.0
11	0.0	0.0	5.473369866252734E-5	1.0946739732505468E-4	0.0
12	0.0	0.0	5.473369866252734E-5	1.0946739732505468E-4	0.0
13	0.0	0.0	5.473369866252734E-5	1.0946739732505468E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTTA	190	0.0	13.9985485	13
TGCGTTT	210	0.0	13.116968	12
GTACCGG	80	2.002278E-6	13.061146	6
CTCGAAC	115	5.326001E-9	12.391375	18
TGGACCG	85	3.9405077E-6	12.295198	5
TAGGACG	295	0.0	12.239034	4
TACGACT	55	0.0030655211	12.092635	4
TATCGAC	55	0.0030692955	12.090648	19
CGCTCAA	55	0.003071814	12.089325	10
AGGACGT	280	0.0	11.876043	5
TCGAACT	120	1.0018994E-8	11.8747425	19
GTAGGAC	520	0.0	11.693015	3
ACCGAGC	65	8.033434E-4	11.690775	8
GTCCTAA	240	0.0	11.490244	1
TGTAGGA	620	0.0	11.339706	2
CGTTTAT	245	0.0	11.243731	14
TAGGACC	355	0.0	11.24104	4
GGACGTG	290	0.0	11.136775	6
CCGTACA	60	0.005872484	11.084308	2
TGGCGAG	240	0.0	11.083397	18
>>END_MODULE