FastQCFastQC Report
Thu 26 May 2016
SRR1513083_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513083_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827028
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC144100.7887125977270191No Hit
CCCATGTACTCTGCGTTGATACCAC135880.7437214974264215No Hit
GAGTACATGGGAAGCAGTGGTATCA58950.32265515361559866No Hit
CATGTACTCTGCGTTGATACCACTG40200.22002946862335993No Hit
GTATCAACGCAGAGTACATGGGAAG35560.19463303244394722No Hit
GCGTTGATACCACTGCTTCCCATGT35190.1926078855934337No Hit
ACGCAGAGTACATGGGAAGCAGTGG33700.18445256449271713No Hit
GTATCAACGCAGAGTACTTTTTTTT31110.17027653653912256No Hit
GCTTCCCATGTACTCTGCGTTGATA30700.16803245489395893No Hit
TATCAACGCAGAGTACATGGGAAGC29510.16151914475311818No Hit
ACTCTGCGTTGATACCACTGCTTCC26740.1463579102235981No Hit
TATCAACGCAGAGTACTTTTTTTTT21720.11888159349500939No Hit
GGTATCAACGCAGAGTACATGGGAA19910.10897479403709193No Hit
GGTATCAACGCAGAGTACTTTTTTT19220.10519816882937755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG3000.011.4230075
TACACCG752.0357102E-411.4230075
GTGCGCC855.3993863E-511.16101311
TTAGACT1303.1710442E-810.9836614
TAGGACG3150.010.8790544
TAAGACC1502.062734E-910.7883954
TTTAGGC803.7017357E-410.7090683
TGGCGAG2600.010.58526518
CCTACAC3150.010.5768583
GTAGGAC4250.010.5270843
GTCCTAC4600.010.3571671
GCCTAAG1752.237357E-1010.3453311
CTACACG1201.4848574E-610.3124374
ACAGCGC1201.5483474E-610.278848
ACATTAG750.002582737510.1638341
TAGGACC3100.010.1333124
AAAGGCG1701.5061232E-910.0791245
CGTGCGC856.686958E-410.04353710
ACACCGA1151.0021473E-59.9330496
AGGACGT3100.09.8262425
Produced by FastQC (version 0.11.5)