##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513083_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1827028 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.616778724792397 31.0 31.0 34.0 27.0 34.0 2 30.770793879458882 31.0 31.0 34.0 27.0 34.0 3 30.78055453994137 33.0 31.0 34.0 27.0 34.0 4 34.01714368909508 37.0 35.0 37.0 30.0 37.0 5 33.953080631495524 37.0 35.0 37.0 30.0 37.0 6 33.910663657043024 37.0 35.0 37.0 30.0 37.0 7 33.83276282574761 37.0 35.0 37.0 29.0 37.0 8 33.842471489216365 37.0 35.0 37.0 29.0 37.0 9 35.4199579864129 39.0 35.0 39.0 30.0 39.0 10 35.33146673176328 39.0 35.0 39.0 29.0 39.0 11 35.33631832681272 39.0 35.0 39.0 29.0 39.0 12 35.249073358481645 39.0 35.0 39.0 29.0 39.0 13 35.224375324297164 39.0 35.0 39.0 29.0 39.0 14 36.417308328060656 40.0 37.0 41.0 29.0 41.0 15 36.39039576842829 40.0 37.0 41.0 28.0 41.0 16 36.34070304341258 40.0 37.0 41.0 28.0 41.0 17 36.25347394785411 40.0 37.0 41.0 27.0 41.0 18 36.176358545134505 40.0 36.0 41.0 27.0 41.0 19 36.23974673622955 40.0 37.0 41.0 27.0 41.0 20 36.14487517432683 40.0 37.0 41.0 27.0 41.0 21 36.162368611756364 40.0 37.0 41.0 27.0 41.0 22 36.15012030466966 40.0 37.0 41.0 27.0 41.0 23 36.08580930341516 40.0 37.0 41.0 27.0 41.0 24 35.952193398240205 40.0 37.0 41.0 27.0 41.0 25 35.937426793678036 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5852056479754033 1101 2 0.5144455213086303 1101 3 0.6335300793221101 1101 4 0.9405650082555539 1101 5 0.682677272405769 1101 6 0.6616294424145437 1101 7 0.3869799904220059 1101 8 0.353333854950705 1101 9 0.42562927195358924 1101 10 0.6052219826270502 1101 11 0.5350001945767602 1101 12 0.6021783085934374 1101 13 0.49715844049877234 1101 14 0.44738019119854044 1101 15 0.45630972655732194 1101 16 0.44324244453455464 1101 17 0.39970693371624577 1101 18 0.2991415021400954 1101 19 0.43195550613747713 1101 20 0.6776417053341675 1101 21 0.6095543294458992 1101 22 0.6559290586545714 1101 23 0.5275543242847647 1101 24 0.46674798730327893 1101 25 0.5942110255887769 1102 1 0.7402383901837908 1102 2 0.7347263164211952 1102 3 0.6436117088120561 1102 4 0.9508649191347516 1102 5 0.796040872369602 1102 6 0.545010995699748 1102 7 0.6546278470020894 1102 8 0.6187859815797481 1102 9 0.6037598016411465 1102 10 0.6817625455088816 1102 11 0.6534804911294643 1102 12 0.691859023626435 1102 13 0.6916213262045687 1102 14 0.8018013580352701 1102 15 0.7614328811754589 1102 16 0.8065453390232662 1102 17 0.8055947189996857 1102 18 0.8241192219391209 1102 19 0.8000786736716705 1102 20 0.9050444151232426 1102 21 0.8699149458002182 1102 22 0.9544966677629745 1102 23 0.8588634959779355 1102 24 0.9257608129494059 1102 25 0.898034178915303 1103 1 0.14726251298507975 1103 2 0.21870847323116038 1103 3 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>>Per sequence quality scores pass #Quality Count 2 43674.0 3 1.0 4 715.0 5 5803.0 6 6723.0 7 5127.0 8 4211.0 9 3699.0 10 3326.0 11 3082.0 12 2761.0 13 2715.0 14 2676.0 15 2568.0 16 2694.0 17 2899.0 18 3228.0 19 3725.0 20 4818.0 21 6319.0 22 8039.0 23 9802.0 24 11261.0 25 12605.0 26 13766.0 27 15178.0 28 17192.0 29 20460.0 30 25945.0 31 32752.0 32 42013.0 33 55581.0 34 73493.0 35 100443.0 36 147592.0 37 248086.0 38 882056.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.51259776157991 18.069174868412897 12.79455962256888 27.623667747438308 2 14.20881030583748 19.421318089930164 38.211529055957286 28.158342548275066 3 18.02287649669299 23.115464021350522 28.509579491939917 30.352079990016573 4 10.80125756146047 15.996963374398204 35.87941728315056 37.32236178099077 5 13.375226775307139 37.324779465461724 35.16142241355714 14.138571345673997 6 34.88976040602446 34.811049226070814 16.665348405453205 13.633841962451527 7 29.652425500984126 30.263097398801538 20.52633710708898 19.558139993125355 8 26.903705877489838 35.93628608517928 18.91110591592448 18.248902121406402 9 26.39364313887812 14.929403494017857 18.61368260371565 40.06327076338837 10 14.684529256961198 29.151322616946835 33.44506574770222 22.71908237838974 11 35.89071462976749 22.308589131672285 21.538512063756183 20.262184174804045 12 23.457968020520468 24.830706693838987 30.096575597788334 21.61474968785221 13 29.438898839108596 20.274326827495305 24.70731392237661 25.57946041101949 14 21.617331610287263 20.765222931400544 26.51235880518687 31.10508665312532 15 25.65620231326504 27.843962982504923 22.15039944653284 24.3494352576972 16 23.36644177740068 27.34553118114762 26.07857895529128 23.209448086160414 17 23.562581571962895 27.339253961441095 26.343911775268698 22.754252691327313 18 23.584760471384122 26.627003100461867 27.108639942777558 22.679596485376454 19 23.992792717819903 26.807103914292252 27.008036196280727 22.192067171607118 20 24.10597976604628 26.292262625422268 26.813546371484183 22.78821123704727 21 23.46860584512115 26.197682794133424 27.06192789601473 23.2717834647307 22 23.54205846872626 26.29160582103832 25.7307496108434 24.43558609939202 23 22.625592219916104 26.41465027279335 26.414705006611843 24.5450525006787 24 23.084206885076497 27.355469878885007 26.305784707808982 23.254538528229514 25 23.43051119085203 25.73288422509124 25.910604544648468 24.926000039408265 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 78.0 1 78.0 2 228.5 3 379.0 4 379.0 5 379.0 6 835.5 7 1292.0 8 1292.0 9 1292.0 10 1745.0 11 2198.0 12 2198.0 13 2198.0 14 3693.0 15 5188.0 16 5188.0 17 5188.0 18 9589.0 19 13990.0 20 13990.0 21 13990.0 22 22533.5 23 31077.0 24 31077.0 25 31077.0 26 44299.0 27 57521.0 28 57521.0 29 57521.0 30 74957.5 31 92394.0 32 92394.0 33 92394.0 34 111340.5 35 130287.0 36 130287.0 37 130287.0 38 150001.0 39 169715.0 40 169715.0 41 169715.0 42 188802.0 43 207889.0 44 207889.0 45 207889.0 46 244739.5 47 281590.0 48 281590.0 49 281590.0 50 274010.0 51 266430.0 52 266430.0 53 266430.0 54 237644.0 55 208858.0 56 208858.0 57 208858.0 58 181876.0 59 154894.0 60 154894.0 61 154894.0 62 128347.5 63 101801.0 64 101801.0 65 101801.0 66 79400.5 67 57000.0 68 57000.0 69 57000.0 70 41990.0 71 26980.0 72 26980.0 73 26980.0 74 18970.5 75 10961.0 76 10961.0 77 10961.0 78 7605.5 79 4250.0 80 4250.0 81 4250.0 82 2924.5 83 1599.0 84 1599.0 85 1599.0 86 1052.0 87 505.0 88 505.0 89 505.0 90 317.0 91 129.0 92 129.0 93 129.0 94 75.0 95 21.0 96 21.0 97 21.0 98 11.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18231795024487857 2 0.06507836770974501 3 0.0 4 0.0 5 0.018062120558634022 6 0.3529228889759763 7 0.0013136087679006562 8 0.030158267963052564 9 0.03240234960821618 10 0.002408282741151203 11 0.0037218915090518594 12 0.009249995073967121 13 0.008538456991354264 14 0.0016967446585383476 15 0.0 16 0.01111094082849305 17 0.022988153438261483 18 0.0640931611338195 19 0.02966566467508982 20 0.0 21 0.0 22 0.0 23 2.1893479465010936E-4 24 2.1893479465010936E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1827028.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.15932093086418 #Duplication Level Percentage of deduplicated Percentage of total 1 81.1145308665538 55.2871134148993 2 12.480557691407864 17.013326741696677 3 3.376757648564828 6.904725248228412 4 1.2952503570537959 3.531335390889844 5 0.5959570988677206 2.031001558137586 6 0.3241434805979443 1.325603971303358 7 0.2055071228976376 0.9805058160211041 8 0.12195949630082643 0.6650141159147658 9 0.08664982173916919 0.5315393707669984 >10 0.3655133426666674 4.245843773692581 >50 0.018517497281047225 0.8633005526868058 >100 0.011032936336473408 1.3913243220374778 >500 0.001368552787667495 0.657072678393444 >1k 0.002012577628922786 2.7125716015915065 >5k 8.050310515691144E-5 0.323460829989925 >10k+ 1.6100621031382289E-4 1.5362606137502832 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 14410 0.7887125977270191 No Hit CCCATGTACTCTGCGTTGATACCAC 13588 0.7437214974264215 No Hit GAGTACATGGGAAGCAGTGGTATCA 5895 0.32265515361559866 No Hit CATGTACTCTGCGTTGATACCACTG 4020 0.22002946862335993 No Hit GTATCAACGCAGAGTACATGGGAAG 3556 0.19463303244394722 No Hit GCGTTGATACCACTGCTTCCCATGT 3519 0.1926078855934337 No Hit ACGCAGAGTACATGGGAAGCAGTGG 3370 0.18445256449271713 No Hit GTATCAACGCAGAGTACTTTTTTTT 3111 0.17027653653912256 No Hit GCTTCCCATGTACTCTGCGTTGATA 3070 0.16803245489395893 No Hit TATCAACGCAGAGTACATGGGAAGC 2951 0.16151914475311818 No Hit ACTCTGCGTTGATACCACTGCTTCC 2674 0.1463579102235981 No Hit TATCAACGCAGAGTACTTTTTTTTT 2172 0.11888159349500939 No Hit GGTATCAACGCAGAGTACATGGGAA 1991 0.10897479403709193 No Hit GGTATCAACGCAGAGTACTTTTTTT 1922 0.10519816882937755 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 5.473369866252734E-5 0.0 0.0 0.0 0.0 9 5.473369866252734E-5 0.0 0.0 5.473369866252734E-5 0.0 10 5.473369866252734E-5 0.0 0.0 5.473369866252734E-5 0.0 11 5.473369866252734E-5 0.0 0.0 5.473369866252734E-5 0.0 12 5.473369866252734E-5 0.0 0.0 5.473369866252734E-5 0.0 13 5.473369866252734E-5 0.0 0.0 5.473369866252734E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACAG 300 0.0 11.423007 5 TACACCG 75 2.0357102E-4 11.423007 5 GTGCGCC 85 5.3993863E-5 11.161013 11 TTAGACT 130 3.1710442E-8 10.983661 4 TAGGACG 315 0.0 10.879054 4 TAAGACC 150 2.062734E-9 10.788395 4 TTTAGGC 80 3.7017357E-4 10.709068 3 TGGCGAG 260 0.0 10.585265 18 CCTACAC 315 0.0 10.576858 3 GTAGGAC 425 0.0 10.527084 3 GTCCTAC 460 0.0 10.357167 1 GCCTAAG 175 2.237357E-10 10.345331 1 CTACACG 120 1.4848574E-6 10.312437 4 ACAGCGC 120 1.5483474E-6 10.27884 8 ACATTAG 75 0.0025827375 10.163834 1 TAGGACC 310 0.0 10.133312 4 AAAGGCG 170 1.5061232E-9 10.079124 5 CGTGCGC 85 6.686958E-4 10.043537 10 ACACCGA 115 1.0021473E-5 9.933049 6 AGGACGT 310 0.0 9.826242 5 >>END_MODULE