FastQCFastQC Report
Thu 26 May 2016
SRR1513082_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513082_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2222901
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC36210.16289524364782776No Hit
CCCATGTACTCTGCGTTGATACCAC29440.13243954634057029No Hit
GTATCAACGCAGAGTACTTTTTTTT26840.12074311901429707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTTA1103.8280632E-812.08831813
GTAGGAC10950.011.5402343
TAGGACC6200.011.4949044
GTCTAGG2650.011.4837081
TGCGCCA1501.7644197E-1011.39730112
TGTAGGA11800.011.3528382
TATACCG600.005870633811.0849495
AAAGGCG1900.011.0016035
CGTGCGC1303.271998E-810.96066910
TGGCGAG9550.010.94252518
GGACGTG7850.010.8908556
GAACCGT700.001492826910.8562816
GTATTGG2300.010.7503761
TGAACCG803.7669725E-410.6890575
TATGGCG10300.010.51396516
AATCCCG1909.094947E-1210.49987619
CCAACGA1451.4444595E-810.48263519
AAGGCGT2001.8189894E-1210.4491716
GGCGAGA6500.010.376819
AGGACGT8450.010.3447025
Produced by FastQC (version 0.11.5)