##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513082_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2222901
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.468625908216335	31.0	30.0	34.0	25.0	34.0
2	29.237278223366673	31.0	30.0	34.0	22.0	34.0
3	29.57054992552525	31.0	30.0	34.0	25.0	34.0
4	32.45073802207116	35.0	33.0	37.0	20.0	37.0
5	32.680609707764766	35.0	33.0	37.0	25.0	37.0
6	33.19111287457246	37.0	35.0	37.0	27.0	37.0
7	33.220474056199535	37.0	35.0	37.0	27.0	37.0
8	33.20864311995901	37.0	35.0	37.0	27.0	37.0
9	34.62933436981674	39.0	35.0	39.0	27.0	39.0
10	34.118631913881906	39.0	34.0	39.0	25.0	39.0
11	34.50374848002678	39.0	35.0	39.0	27.0	39.0
12	34.380385361291395	39.0	35.0	39.0	27.0	39.0
13	34.450900422465956	39.0	35.0	39.0	27.0	39.0
14	34.841736091710786	40.0	34.0	41.0	18.0	41.0
15	35.35016539198102	40.0	35.0	41.0	26.0	41.0
16	34.92307394706287	40.0	34.0	41.0	22.0	41.0
17	34.44260270700315	39.0	34.0	41.0	17.0	41.0
18	33.82784073604717	39.0	33.0	41.0	11.0	41.0
19	34.755901859776934	39.0	34.0	41.0	19.0	41.0
20	35.025668259630095	40.0	35.0	41.0	24.0	41.0
21	35.064627709466144	40.0	35.0	41.0	24.0	41.0
22	34.65237093329842	40.0	34.0	41.0	18.0	41.0
23	32.9151163277177	39.0	32.0	41.0	9.0	41.0
24	34.0534171337365	39.0	34.0	41.0	15.0	41.0
25	33.79397373072395	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.521048337771763
1101	2	0.8619962651875959
1101	3	0.5882252488441964
1101	4	0.6381332766144396
1101	5	0.8318457799011938
1101	6	0.48570312974501917
1101	7	0.5105772266887456
1101	8	0.5437435350704973
1101	9	0.6278998166900251
1101	10	0.9561163388028149
1101	11	0.6180410065400892
1101	12	0.6483269203694348
1101	13	0.6025926488337916
1101	14	-0.371266113371739
1101	15	0.2367600948570825
1101	16	0.9006653880339712
1101	17	1.5565327248543923
1101	18	-5.091691285156287
1101	19	-1.1606435563663524
1101	20	-0.06148835620935955
1101	21	0.3988624187409826
1101	22	1.0422294533655645
1101	23	2.8552992200646656
1101	24	1.8125182910131343
1101	25	1.9483366410806866
1102	1	0.8069836174728025
1102	2	1.074443309503522
1102	3	0.8472042345259965
1102	4	1.4163961975803616
1102	5	1.2707160807403142
1102	6	0.8827077772485552
1102	7	0.8795642565516602
1102	8	0.7834424528629427
1102	9	0.9025798726457026
1102	10	1.2366345034035504
1102	11	0.7974024886141606
1102	12	0.8626021374407813
1102	13	0.7730690676704342
1102	14	1.7380695566046782
1102	15	1.1276078498896638
1102	16	1.4120736501151612
1102	17	1.003099141282199
1102	18	1.3903684229990532
1102	19	1.1965440520588189
1102	20	1.2719064871169579
1102	21	1.1952667360795033
1102	22	1.5080836724722673
1102	23	3.0457722797927076
1102	24	1.9679831408145745
1102	25	2.3434363204184123
1103	1	0.5033218012523868
1103	2	0.8692251252408099
1103	3	0.6467689276084769
1103	4	1.098072352500104
1103	5	0.828008818484065
1103	6	0.38942753278632125
1103	7	0.4446114202309417
1103	8	0.4480693914495646
1103	9	0.573941664487279
1103	10	0.9659472934585125
1103	11	0.633236030401946
1103	12	0.792964393656348
1103	13	0.6347300358759185
1103	14	1.2960387515403653
1103	15	0.7660666304200276
1103	16	0.9504222490946788
1103	17	1.3307532166646254
1103	18	1.5381033998963716
1103	19	0.9247647209290122
1103	20	0.8858030299304289
1103	21	0.8024602897674669
1103	22	1.190490159595818
1103	23	2.6069939203201997
1103	24	1.5053690616688442
1103	25	1.6349869068296456
1104	1	0.367166411818836
1104	2	0.8121786176317407
1104	3	0.53755319758341
1104	4	1.166728898062921
1104	5	0.8226516276116413
1104	6	0.46593986071725624
1104	7	0.39770441390678
1104	8	0.38353803774288053
1104	9	0.5289002679051436
1104	10	0.7737806844214106
1104	11	0.43507366661173563
1104	12	0.4408047473399179
1104	13	0.3474595497449826
1104	14	1.2593990064501597
1104	15	0.639634205526761
1104	16	0.9872938490093333
1104	17	1.4680640277665518
1104	18	2.0265480825115674
1104	19	1.168399563024792
1104	20	0.6318849664347539
1104	21	0.7351599652021577
1104	22	1.117835395910845
1104	23	2.5565875493364416
1104	24	1.575781539971274
1104	25	1.6558965325079313
1105	1	0.34624865097785573
1105	2	0.8029889144546551
1105	3	0.4707582212115149
1105	4	0.5404360184782391
1105	5	0.5914027701959554
1105	6	0.36376243140900755
1105	7	0.21163631758294343
1105	8	0.24111797688547654
1105	9	0.44618458742600353
1105	10	0.9495535354117095
1105	11	0.5768939383697145
1105	12	0.4774505002670182
1105	13	0.44276198543315104
1105	14	1.0995542834651815
1105	15	0.5513034287983345
1105	16	0.8634672502342084
1105	17	1.2908432073206413
1105	18	1.1061904238481688
1105	19	0.8349663163964038
1105	20	0.6075610283537785
1105	21	0.4975092330047417
1105	22	0.956935396746033
1105	23	2.5938731494797196
1105	24	1.3944624184535073
1105	25	1.2946756353484048
1106	1	-0.835721323525469
1106	2	-0.3326077324656218
1106	3	-0.5737147873913884
1106	4	-3.0715740384297376
1106	5	-0.874701001758833
1106	6	-0.777526036681401
1106	7	-0.8096669599061599
1106	8	-0.8268771200411109
1106	9	-0.7556196957066419
1106	10	-0.6433344091675579
1106	11	-0.7376032217445854
1106	12	-0.5682163802501776
1106	13	-0.6476085031305203
1106	14	-0.04211095639367812
1106	15	-0.632686653535437
1106	16	-1.7754344978639338
1106	17	-6.632486179254915
1106	18	-7.487475321313298
1106	19	-3.553048907577246
1106	20	-2.097525674044256
1106	21	-1.6066240572539598
1106	22	-1.9715891716242595
1106	23	-5.475148303986682
1106	24	-2.659759480901492
1106	25	-1.2545078866832142
1107	1	0.49191831313584444
1107	2	0.9160406098409801
1107	3	0.6432497288392085
1107	4	0.9621170449744412
1107	5	0.9673599377581823
1107	6	0.5309533090570824
1107	7	0.48667399283462487
1107	8	0.47947107428526436
1107	9	0.4864234546979418
1107	10	0.9256268168643729
1107	11	0.6201858906889015
1107	12	0.7342150608253561
1107	13	0.6003337845808261
1107	14	1.4762929036862928
1107	15	0.8791560938591658
1107	16	-5.529176193654369
1107	17	-0.7865084077741642
1107	18	0.7962230036764666
1107	19	0.745471998565506
1107	20	0.5936137737061316
1107	21	0.5633263536273105
1107	22	0.7768972582740403
1107	23	2.6408182115512417
1107	24	1.6700650680736615
1107	25	1.2832550930718298
1108	1	0.2940901997875969
1108	2	0.5499555986552984
1108	3	0.38332430005471707
1108	4	0.8733306915068795
1108	5	0.676720489585179
1108	6	0.24582094514955344
1108	7	0.28700210618287514
1108	8	0.33597741283858795
1108	9	0.42704053150630017
1108	10	0.8445473985198504
1108	11	0.385797374656093
1108	12	0.3611539124286338
1108	13	0.1553337845808258
1108	14	0.758433097571455
1108	15	0.4892325293550641
1108	16	0.8057716064947726
1108	17	0.8196810031132316
1108	18	0.6620376942059281
1108	19	0.6252277778347093
1108	20	0.48320736058159497
1108	21	0.6162570023968854
1108	22	0.9235210464917856
1108	23	2.6100855493588497
1108	24	1.4683256944718721
1108	25	1.5690753764424556
1109	1	0.3727764494580974
1109	2	0.8062129749158586
1109	3	0.5857347371591963
1109	4	0.53096299957555
1109	5	0.31515207929220423
1109	6	0.34789037539722756
1109	7	0.2762022651023699
1109	8	0.2129747319412303
1109	9	0.26396709539996266
1109	10	0.7752154483045146
1109	11	0.44646010499928934
1109	12	0.500508897753555
1109	13	0.3442524998487926
1109	14	-0.7593441249784263
1109	15	0.08960435294051905
1109	16	-0.12587048896691755
1109	17	0.8780829708324731
1109	18	1.7197931427615885
1109	19	0.9533038562779836
1109	20	0.8080974633862041
1109	21	0.6753057263314659
1109	22	0.48322822119514086
1109	23	-4.230813369874774
1109	24	-1.2550152276784345
1109	25	0.1554729198276661
1110	1	0.44320006582201543
1110	2	0.9223681671355557
1110	3	0.6304300281994948
1110	4	1.1319628620044355
1110	5	0.867406673985748
1110	6	0.36547523316117747
1110	7	0.3705641238823887
1110	8	0.31610078672548525
1110	9	0.405771166020223
1110	10	0.7129302452228572
1110	11	0.4752979955271144
1110	12	0.4940731441742159
1110	13	0.3296863092687303
1110	14	1.0965944377525005
1110	15	0.5642447529486674
1110	16	0.9364588600690027
1110	17	1.2600042311923545
1110	18	1.8705356240106141
1110	19	0.9746520184400538
1110	20	0.5346435234111198
1110	21	0.4887288131018792
1110	22	0.8108685954580679
1110	23	2.2422738664795077
1110	24	1.3204838308834255
1110	25	1.5535184307527246
1111	1	0.5386754846252622
1111	2	0.9276640596718693
1111	3	0.6927887492575984
1111	4	1.235956766883497
1111	5	0.9075963776930465
1111	6	0.4553128856573565
1111	7	0.3411766860577288
1111	8	0.20566736640602556
1111	9	0.29638243002050757
1111	10	0.5612797668831604
1111	11	0.2459255875447255
1111	12	0.430153304593361
1111	13	0.2845145436939376
1111	14	-0.2943227796407726
1111	15	0.032211023083768
1111	16	0.5331149249727147
1111	17	1.0367973669972201
1111	18	1.7679908227920933
1111	19	0.8348221802014848
1111	20	0.6978239703740527
1111	21	0.6540647978212206
1111	22	1.0393571730930304
1111	23	0.7607120484546641
1111	24	0.8707198970853156
1111	25	0.3758055127135691
1112	1	0.35551738826570656
1112	2	0.6053336543107619
1112	3	0.5010819852689572
1112	4	1.020673677433578
1112	5	0.8114537550646617
1112	6	0.18105941573388407
1112	7	0.15658974096588452
1112	8	0.16010264656450346
1112	9	0.2809347305119587
1112	10	0.5479367750551702
1112	11	0.197676071862098
1112	12	0.24145882111928785
1112	13	0.1492524466864893
1112	14	0.6193215366522153
1112	15	0.287867927129156
1112	16	0.8133640318619086
1112	17	0.8994411677991252
1112	18	1.0699082310931658
1112	19	0.47067882740118705
1112	20	0.3015334493681081
1112	21	0.45150765351328914
1112	22	0.38286995560078907
1112	23	2.051940408434554
1112	24	0.9282055089706631
1112	25	0.9409492524535636
1113	1	0.40599573897797825
1113	2	0.8667419673070427
1113	3	0.5140126750433289
1113	4	0.6661140283831841
1113	5	0.8230713505284228
1113	6	0.3344915192129392
1113	7	0.28001738147213473
1113	8	0.21532451822694298
1113	9	0.3447542742052363
1113	10	0.6656796072112812
1113	11	0.27644657444958654
1113	12	0.3547341659032881
1113	13	0.22386696708459652
1113	14	1.0704168353102261
1113	15	0.6009761039144692
1113	16	0.9505736806543297
1113	17	1.3398930502449389
1113	18	1.799166693973099
1113	19	0.9459860759913497
1113	20	0.6416429340882317
1113	21	0.6173648151657716
1113	22	0.817867595126728
1113	23	0.9725301270866851
1113	24	0.8440507392652208
1113	25	1.2232764605931976
1114	1	-0.26570844832358276
1114	2	-3.0834326223376642
1114	3	-0.981688083292994
1114	4	0.19926395784780482
1114	5	0.4709032943125635
1114	6	0.1631967642213823
1114	7	0.07948039929834749
1114	8	0.0884596864301912
1114	9	0.17230943300376111
1114	10	0.4079416846952668
1114	11	0.18691999381579194
1114	12	0.16124610704478215
1114	13	0.0953328302912908
1114	14	0.9153002835253403
1114	15	0.41899489845248183
1114	16	0.5836535929028699
1114	17	0.9455684443427685
1114	18	1.2011925459355908
1114	19	0.5452228494736744
1114	20	0.33717200127237845
1114	21	0.3204591907300909
1114	22	0.43351947095337096
1114	23	2.0969888862339374
1114	24	0.956042499126248
1114	25	0.8483494086871914
1115	1	0.5216866183073776
1115	2	0.8588286282741606
1115	3	0.5758636561305543
1115	4	0.753495693207924
1115	5	0.8333135987924933
1115	6	0.33960026246209907
1115	7	0.13889877522069582
1115	8	0.26747670664573775
1115	9	0.26525858014597503
1115	10	0.47881038061245107
1115	11	0.4324196336484505
1115	12	0.2435212564218716
1115	13	0.17799123465762534
1115	14	1.1017665339986351
1115	15	0.4378286489026948
1115	16	0.9249687116195702
1115	17	1.3736042394352523
1115	18	1.7886820410548623
1115	19	1.0182356954421081
1115	20	0.5387929851756681
1115	21	0.5267940955627921
1115	22	0.8136893729693782
1115	23	2.59412082291837
1115	24	1.3317662969523099
1115	25	1.08188156516605
1116	1	1.1621789087830408
1116	2	1.0644636720784746
1116	3	1.1549755784001086
1116	4	1.905344421835835
1116	5	1.3383593650205015
1116	6	1.146784733264795
1116	7	1.0112196209369557
1116	8	1.0687895164387626
1116	9	1.119559193529561
1116	10	1.7172753468376456
1116	11	1.1896933362788218
1116	12	1.3176533960678114
1116	13	1.1794842236797152
1116	14	2.1595785088791146
1116	15	1.3508580793498126
1116	16	1.8250086097056908
1116	17	2.0237626880639397
1116	18	2.652042973130463
1116	19	1.9145336633230485
1116	20	1.6668783785171257
1116	21	1.643011347899936
1116	22	1.9465144785871402
1116	23	3.283988314644027
1116	24	2.2915646862485417
1116	25	2.3908114122776354
1201	1	0.7167911891386254
1201	2	1.0200315137636622
1201	3	0.7542212404303896
1201	4	1.296528168718993
1201	5	1.1059473611679493
1201	6	0.6882404355809939
1201	7	0.6057735443474783
1201	8	0.6408206415899187
1201	9	0.8070723397968038
1201	10	1.0693509972699005
1201	11	0.7391903755603622
1201	12	0.8358653040190873
1201	13	0.7633023273134398
1201	14	1.6133799480928417
1201	15	1.017254603113166
1201	16	1.432442781773588
1201	17	1.8245648030147876
1201	18	2.417534512740964
1201	19	1.5624199172061495
1201	20	1.2483810025609117
1201	21	1.0567288550485756
1201	22	1.4383145724045434
1201	23	3.004876862299774
1201	24	1.85586618613317
1201	25	2.0139928860097385
1202	1	0.8120451596355238
1202	2	1.194306505794735
1202	3	0.894778309442934
1202	4	1.5194629023925472
1202	5	1.2651714342257492
1202	6	0.9047410418766049
1202	7	0.7829199787048893
1202	8	0.7509707531548884
1202	9	0.784816197151379
1202	10	1.4412867397930782
1202	11	0.9521190955700831
1202	12	1.0868964994894554
1202	13	1.0737345552937327
1202	14	1.9088394675912426
1202	15	1.3054647001533226
1202	16	1.4859040889403659
1202	17	1.9501325684621875
1202	18	2.6214815136315153
1202	19	1.8295872843715486
1202	20	1.4340055527684328
1202	21	1.2894246195232668
1202	22	1.6530260315559957
1202	23	3.349609155674557
1202	24	2.073451387919519
1202	25	2.3316301733544265
1203	1	0.8342492310102436
1203	2	1.0935234473695843
1203	3	0.8384811043795075
1203	4	1.5293843981399604
1203	5	1.1278055263673892
1203	6	0.9154657677400238
1203	7	0.7485593220998155
1203	8	0.6974598868965032
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1203	10	1.2112656421885504
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2211	15	-0.591293886206465
2211	16	-0.6614996211392636
2211	17	-0.2828036817950803
2211	18	-5.7915556174784975
2211	19	-2.0661499691101426
2211	20	-1.2003678866735399
2211	21	-0.8922863982357825
2211	22	-0.538846063712036
2211	23	-5.095189010824324
2211	24	-2.233851345579847
2211	25	-1.3996176029750274
2212	1	0.23123004505020006
2212	2	0.41291632249776455
2212	3	0.1823818712438694
2212	4	0.687720427718645
2212	5	0.5728562636165364
2212	6	0.04765006567686925
2212	7	0.02147001462608955
2212	8	-0.08248572287677547
2212	9	-0.2039569456567989
2212	10	-3.929258207841066
2212	11	-1.2344790022542114
2212	12	-0.38339864752381203
2212	13	-0.14436214852445772
2212	14	0.5372234750786689
2212	15	0.1450515075168397
2212	16	0.4510493226258774
2212	17	0.9099884882096134
2212	18	1.4065672960874664
2212	19	0.7010095001491905
2212	20	0.164703346745533
2212	21	0.13337766491579828
2212	22	0.2976362041754754
2212	23	1.575315550965911
2212	24	0.7642944485373633
2212	25	0.6966505067295046
2213	1	-1.4968777679539613
2213	2	-4.006690360525088
2213	3	-1.7978691297055676
2213	4	-3.5380161140416355
2213	5	-1.403301660795627
2213	6	-1.004104635290517
2213	7	-0.8960276018454678
2213	8	-0.7837366860607844
2213	9	-0.75749755375967
2213	10	-0.7402714012258897
2213	11	-0.8732455768041518
2213	12	-0.8117675720108508
2213	13	-1.0997020472998642
2213	14	-6.304049597443537
2213	15	-2.8176763356069046
2213	16	-1.928723614404582
2213	17	-0.8325346199247221
2213	18	-0.03998866266739043
2213	19	-0.7402793410948973
2213	20	-0.8464223809842935
2213	21	-1.0398084517184145
2213	22	-0.824671435637768
2213	23	-5.901528776633924
2213	24	-3.1706811066309655
2213	25	-2.187634570560423
2214	1	0.09821196392949716
2214	2	0.2828977526981227
2214	3	0.08832909391857413
2214	4	0.3571408544982475
2214	5	0.16059009648834177
2214	6	-0.03437208776831824
2214	7	-0.28454822938759605
2214	8	-0.34759241777822325
2214	9	-0.2612828945084047
2214	10	0.2600315787691301
2214	11	-0.17073474423172996
2214	12	-0.042626209015914185
2214	13	-0.22399954875250927
2214	14	0.48049925289264195
2214	15	-0.010185175982101669
2214	16	0.3755539650757882
2214	17	0.6882217509559894
2214	18	1.2991198290732981
2214	19	0.337765649359163
2214	20	0.18621694830930835
2214	21	0.008326353627310823
2214	22	0.43257186144864335
2214	23	1.9006991639321953
2214	24	0.8789460204546202
2214	25	0.6325566803734191
2215	1	-2.2382706139683854
2215	2	-4.4266593041745
2215	3	-2.2624988142008213
2215	4	-3.9972015276824813
2215	5	-4.8848930955847045
2215	6	-2.2688974122575267
2215	7	-1.5252200551256294
2215	8	-1.8022691569175606
2215	9	-1.5200996655848513
2215	10	-2.8938336302551484
2215	11	-1.9137569773726213
2215	12	-1.515730004618419
2215	13	-1.522295908741043
2215	14	-0.7012818550793511
2215	15	-1.1920880544163666
2215	16	-1.948328768413134
2215	17	-6.378435416468157
2215	18	-2.6196313480957656
2215	19	-1.8703116548213288
2215	20	-1.6670301548311741
2215	21	-1.6564945119502212
2215	22	-1.4471967992400536
2215	23	-6.111951206620155
2215	24	-3.178406557033817
2215	25	-2.883252024535885
2216	1	-0.04944389531582871
2216	2	0.27551388637080265
2216	3	0.07088635849752478
2216	4	-2.248201183788794
2216	5	-0.334804835458101
2216	6	-0.19188320871553088
2216	7	-0.2817411031288799
2216	8	-0.17102165314394568
2216	9	-0.11301001634087271
2216	10	0.28850894957803064
2216	11	-0.14127895138012292
2216	12	-0.96640246219728
2216	13	-0.22660528074321462
2216	14	-5.934755809628104
2216	15	-2.1857042641750866
2216	16	-0.5914784571882379
2216	17	0.2504224812868543
2216	18	0.9510463533623295
2216	19	0.4357441820580661
2216	20	0.010635892649240475
2216	21	-0.2098301142270671
2216	22	0.30772843995921306
2216	23	1.531468224461129
2216	24	0.6252425790470681
2216	25	0.7892099945263453
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	92070.0
3	5.0
4	822.0
5	4619.0
6	5568.0
7	5084.0
8	4883.0
9	4543.0
10	4379.0
11	4252.0
12	4207.0
13	4279.0
14	4194.0
15	4451.0
16	4525.0
17	4937.0
18	5513.0
19	6407.0
20	7984.0
21	10001.0
22	12376.0
23	15189.0
24	17995.0
25	21278.0
26	24577.0
27	28717.0
28	31575.0
29	38767.0
30	46034.0
31	51880.0
32	67365.0
33	89029.0
34	116848.0
35	156582.0
36	224665.0
37	316305.0
38	780996.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.118239094536605	18.221757293913097	12.384330194767035	25.275673416783263
2	14.174517920842074	19.6329960601961	42.01287344651381	24.17961257244802
3	19.33856300445686	23.92142533138451	28.988944249641797	27.75106741451684
4	10.837714405541412	16.220099547800448	39.37187056895964	33.57031547769849
5	12.615662927112442	37.65578100882463	36.384602576737365	13.343953487325566
6	32.67557851258262	36.607574319125675	17.89521756932477	12.821629598966933
7	28.764929053251453	31.051305909709377	21.61756606938455	18.566198967654618
8	25.43835848812948	36.638035959592145	20.11038323211571	17.81322232016267
9	26.262075830070593	15.414989171182611	19.645945144491478	38.676989854255325
10	13.864374485580042	29.207263373568903	35.11623584768534	21.81212629316571
11	34.93373192566169	22.59800727832951	23.670049062132172	18.79821173387663
12	22.93103564639318	24.69996962913803	31.665965512198962	20.703029212269826
13	28.816932991870768	21.47398807520575	25.68680947864569	24.02226945427779
14	20.696576645163557	21.480749562761055	28.345682211990358	29.476991580085034
15	23.603168332512933	29.890114439004112	23.218240409197954	23.288476819285002
16	22.48270735760145	28.165600813866693	27.35200409273077	21.99968773580109
17	21.72499163057916	28.068967627440184	28.129668426944853	22.0763723150358
18	21.392646892080865	27.519666900035816	29.662283646101983	21.425402561781333
19	23.173942038820183	27.216473783730518	27.671328916780592	21.93825526066871
20	23.309158745957152	27.211439298358027	27.97622797244835	21.503173983236472
21	23.21094626044166	26.686279556577098	27.417218185450704	22.68555599753054
22	22.714823675110747	27.39898512610321	27.50941881458841	22.376772384197633
23	21.516442958909142	26.82252187917216	29.233156896017178	22.427878265901523
24	22.63367633260053	27.18291319110827	27.82465859459557	22.35875188169563
25	22.72484277295587	26.84087039163664	27.826981852741167	22.607304982666328
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	198.0
1	198.0
2	324.0
3	450.0
4	450.0
5	450.0
6	956.5
7	1463.0
8	1463.0
9	1463.0
10	2508.5
11	3554.0
12	3554.0
13	3554.0
14	6457.0
15	9360.0
16	9360.0
17	9360.0
18	16894.5
19	24429.0
20	24429.0
21	24429.0
22	38771.5
23	53114.0
24	53114.0
25	53114.0
26	75309.5
27	97505.0
28	97505.0
29	97505.0
30	125081.0
31	152657.0
32	152657.0
33	152657.0
34	177984.0
35	203311.0
36	203311.0
37	203311.0
38	224907.5
39	246504.0
40	246504.0
41	246504.0
42	263268.0
43	280032.0
44	280032.0
45	280032.0
46	292029.5
47	304027.0
48	304027.0
49	304027.0
50	292243.0
51	280459.0
52	280459.0
53	280459.0
54	250077.5
55	219696.0
56	219696.0
57	219696.0
58	188245.5
59	156795.0
60	156795.0
61	156795.0
62	127027.5
63	97260.0
64	97260.0
65	97260.0
66	75000.5
67	52741.0
68	52741.0
69	52741.0
70	38598.0
71	24455.0
72	24455.0
73	24455.0
74	16986.0
75	9517.0
76	9517.0
77	9517.0
78	6522.0
79	3527.0
80	3527.0
81	3527.0
82	2406.0
83	1285.0
84	1285.0
85	1285.0
86	839.5
87	394.0
88	394.0
89	394.0
90	265.5
91	137.0
92	137.0
93	137.0
94	81.0
95	25.0
96	25.0
97	25.0
98	15.5
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10112911011331588
2	0.04332176736615801
3	0.031895257593568045
4	0.029331040833577387
5	0.031985230111462454
6	0.01758962724835699
7	0.01749965473046258
8	0.020783651633608513
9	0.0265418927788507
10	0.03319985910303698
11	0.01857932494519549
12	0.01691483336414892
13	0.013720808978897396
14	0.019434063865192377
15	0.018309407391512263
16	0.01907417379361474
17	0.023617785947282404
18	0.017319709694673762
19	0.029465999610418996
20	0.02055872033887249
21	0.024877401197804128
22	0.03387465298724505
23	0.026182002707273062
24	0.03760851247986303
25	0.028656246949369312
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2222901.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.91366163062035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.97068353862633	66.12320063843049
2	9.81221459557615	15.093867065023566
3	2.304042735960359	5.316370901284315
4	0.8027931607032505	2.4698304608682418
5	0.3602836154592715	1.3855366045245476
6	0.20216858931299936	0.932971588245593
7	0.1238460532291886	0.6667817402650328
8	0.08358596132250215	0.5143121876983241
9	0.056771714536831455	0.3929868397868423
>10	0.2588888768035584	3.4061949990939207
>50	0.014436000611186758	0.7456219387705412
>100	0.008336843481404649	1.2751179597142999
>500	0.001062716932875527	0.5623536600200019
>1k	8.855974440621709E-4	1.1148534162743746
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3621	0.16289524364782776	No Hit
CCCATGTACTCTGCGTTGATACCAC	2944	0.13243954634057029	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2684	0.12074311901429707	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.3495877684161373E-4	4.498625894720457E-5
10	0.0	0.0	0.0	2.2493129473602288E-4	4.498625894720457E-5
11	0.0	0.0	0.0	2.6991755368322747E-4	4.498625894720457E-5
12	0.0	0.0	0.0	2.6991755368322747E-4	4.498625894720457E-5
13	0.0	0.0	0.0	2.6991755368322747E-4	4.498625894720457E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTTA	110	3.8280632E-8	12.088318	13
GTAGGAC	1095	0.0	11.540234	3
TAGGACC	620	0.0	11.494904	4
GTCTAGG	265	0.0	11.483708	1
TGCGCCA	150	1.7644197E-10	11.397301	12
TGTAGGA	1180	0.0	11.352838	2
TATACCG	60	0.0058706338	11.084949	5
AAAGGCG	190	0.0	11.001603	5
CGTGCGC	130	3.271998E-8	10.960669	10
TGGCGAG	955	0.0	10.942525	18
GGACGTG	785	0.0	10.890855	6
GAACCGT	70	0.0014928269	10.856281	6
GTATTGG	230	0.0	10.750376	1
TGAACCG	80	3.7669725E-4	10.689057	5
TATGGCG	1030	0.0	10.513965	16
AATCCCG	190	9.094947E-12	10.499876	19
CCAACGA	145	1.4444595E-8	10.482635	19
AAGGCGT	200	1.8189894E-12	10.449171	6
GGCGAGA	650	0.0	10.3768	19
AGGACGT	845	0.0	10.344702	5
>>END_MODULE