FastQCFastQC Report
Thu 26 May 2016
SRR1513082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513082_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2222901
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC43230.19447559742876538No Hit
GTATCAACGCAGAGTACTTTTTTTT40820.18363390902248908No Hit
CCCATGTACTCTGCGTTGATACCAC38990.17540142363515066No Hit
TATCAACGCAGAGTACTTTTTTTTT27910.12555664872164796No Hit
GGTATCAACGCAGAGTACTTTTTTT25140.11309545499327231No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCGC701.1039088E-412.1992718
GTAGGAC11900.011.9967813
TAGGACC5900.011.7758244
TGTGCGA658.125889E-411.67504510
ATACCGT752.039522E-411.4209366
GGACGTG9100.011.4000196
TGTAGGA13000.011.3543882
AGGACGT9450.011.1792235
TGTACTG5100.011.010385
GTACTAG1408.003553E-910.8871561
TAGGACG9800.010.6828484
TGGCGAG8650.010.64343618
CTGGACT4050.010.574944
GGCGAGA5300.010.56223419
GTCCTAC10600.010.5148351
CGTGAAA5350.010.4635229
GACGTGA4950.010.35113057
ATGGCGA9100.010.32850317
GACGTGG5200.010.2184237
AAGGCGT1409.436371E-810.1972646
Produced by FastQC (version 0.11.5)