FastQCFastQC Report
Thu 26 May 2016
SRR1513081_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513081_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011615
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT28440.14137894179552252No Hit
GTACATGGGAAGCAGTGGTATCAAC27430.13635810033232004No Hit
GTCCTACAGTGTGCATTTCTCATTT22760.11314292247771068No Hit
CCCATGTACTCTGCGTTGATACCAC21580.10727698888703852No Hit
CTGTAGGACGTGGAATATGGCAAGA20800.10339950736100098No Hit
GTATCAACGCAGAGTACTTTTTTTT20500.10190816831252501No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAT401.2868377E-518.9977367
TAGGACC7600.014.2511394
TCGAGAC400.00528137614.2493663
TGTAGGA17600.012.6853562
GTAGGAC17050.012.6475313
GTCCTAC16750.012.3219311
CGAATTG550.003071724412.08946914
TAGGACA6750.011.9639194
GGCGAGG4950.011.8984619
TGGCGAG12950.011.73692918
CTGTAGG17000.011.6931411
GCGTTTA658.033247E-411.69091413
GGCGAGA8450.011.46694519
ACTGCGC752.0766893E-411.3986428
TCCTACA19100.011.3409392
AACCGTG855.3308948E-511.1751397
AGGACGT11100.011.1270155
GATCTCG600.00587862111.0828419
TACCGTG600.005881886511.0820147
GGACGTG11150.011.0754646
Produced by FastQC (version 0.11.5)