##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513081_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2011615
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.37587112842169	31.0	30.0	34.0	25.0	34.0
2	29.11053655893399	31.0	30.0	34.0	20.0	34.0
3	29.453419764716408	31.0	30.0	34.0	24.0	34.0
4	32.3354931236842	35.0	33.0	37.0	19.0	37.0
5	32.568275241534785	35.0	33.0	37.0	24.0	37.0
6	33.07711018261447	37.0	35.0	37.0	27.0	37.0
7	33.09579019842266	37.0	35.0	37.0	27.0	37.0
8	33.08629881960514	37.0	35.0	37.0	27.0	37.0
9	34.492481911300125	39.0	35.0	39.0	27.0	39.0
10	33.992220181297114	39.0	34.0	39.0	23.0	39.0
11	34.37161832656845	39.0	35.0	39.0	27.0	39.0
12	34.20458139355692	39.0	35.0	39.0	25.0	39.0
13	34.30185000608964	39.0	35.0	39.0	27.0	39.0
14	34.61235126999948	40.0	34.0	41.0	17.0	41.0
15	35.17399402967268	40.0	34.0	41.0	25.0	41.0
16	34.767105534607765	40.0	34.0	41.0	19.0	41.0
17	34.305592769988294	39.0	33.0	41.0	17.0	41.0
18	33.7154500239857	39.0	32.0	41.0	11.0	41.0
19	34.61898723165218	39.0	34.0	41.0	18.0	41.0
20	34.877894129840946	40.0	34.0	41.0	22.0	41.0
21	34.893978718591775	40.0	35.0	41.0	22.0	41.0
22	34.48117756131268	40.0	34.0	41.0	17.0	41.0
23	32.805427977023434	39.0	32.0	41.0	8.0	41.0
24	33.90218406603649	39.0	33.0	41.0	8.0	41.0
25	33.636246498460196	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5982270062654678
1101	2	0.9702290205194828
1101	3	0.6589287973093647
1101	4	0.7308838967102105
1101	5	0.8973257473878675
1101	6	0.5624036377907586
1101	7	0.5750304183201678
1101	8	0.535275760952004
1101	9	0.7000773956554767
1101	10	1.057815047945084
1101	11	0.6216080328300748
1101	12	0.6867086304440022
1101	13	0.6079982300221971
1101	14	-0.11412254502410235
1101	15	0.3636460205430154
1101	16	1.0565087992043587
1101	17	1.5341782108703441
1101	18	-4.330049644755334
1101	19	-0.7582662088008547
1101	20	0.21694869302247355
1101	21	0.5808744965357633
1101	22	1.0049030889774642
1101	23	2.7765216891876463
1101	24	1.768205154470074
1101	25	1.9205051847868972
1102	1	0.7766399559252228
1102	2	1.084576870134594
1102	3	0.8064033295549926
1102	4	1.4551861546481675
1102	5	1.24828696064057
1102	6	0.7469044968624203
1102	7	0.7642680091314844
1102	8	0.6944838930461117
1102	9	0.8412534782886993
1102	10	1.1891726855656586
1102	11	0.8681015661174101
1102	12	0.8470328792636224
1102	13	0.9057587249015882
1102	14	1.7615860199880018
1102	15	1.2501589336753085
1102	16	1.5628994130009204
1102	17	1.3053928840848954
1102	18	1.547578302807473
1102	19	1.2564081895107648
1102	20	1.2094377347855314
1102	21	1.2385944729958496
1102	22	1.6096489525812387
1102	23	3.128629458712922
1102	24	1.9812515443397913
1102	25	2.366702439816187
1103	1	0.9090286541926851
1103	2	1.1573099577780894
1103	3	0.9716543144323602
1103	4	1.4307311201245128
1103	5	1.3077892269175209
1103	6	0.9374422610848967
1103	7	0.816036340558604
1103	8	0.8184769883144654
1103	9	0.9568602898276453
1103	10	1.2051421014168895
1103	11	0.9779943945157683
1103	12	1.1445759808860245
1103	13	0.9478572549985955
1103	14	1.8115400590442192
1103	15	1.2673684478024754
1103	16	1.6191750939430136
1103	17	2.100784081611053
1103	18	2.0933534966059213
1103	19	1.5303191057644199
1103	20	1.4597808533520578
1103	21	1.4454498548741057
1103	22	1.7911673581389138
1103	23	3.2347450176573034
1103	24	2.2551268779272817
1103	25	2.367566166676866
1104	1	0.6044672320083109
1104	2	1.0541941391704661
1104	3	0.8640420087053009
1104	4	1.3792163234457817
1104	5	1.302957419380796
1104	6	0.8417961039633255
1104	7	0.7018900769861105
1104	8	0.7174007694513591
1104	9	0.7508074901682917
1104	10	1.1722987971801402
1104	11	0.8209867300490856
1104	12	0.9091171773944353
1104	13	0.6293358583624453
1104	14	1.6199411661338488
1104	15	1.0826675749048817
1104	16	1.3488695797850383
1104	17	1.8846354761181843
1104	18	2.4220547953072185
1104	19	1.5677919607782584
1104	20	0.9648213047039889
1104	21	1.0465175576631154
1104	22	1.463531633208106
1104	23	2.854013699794841
1104	24	2.0102684576590306
1104	25	1.9470818149630063
1105	1	0.3234307070843343
1105	2	0.8170656467056219
1105	3	0.5590332722929396
1105	4	0.7107661125958273
1105	5	0.7419306389167559
1105	6	0.43879212217538566
1105	7	0.4578220169736795
1105	8	0.33586358089605994
1105	9	0.6188591022043823
1105	10	0.9533244015886737
1105	11	0.5624649478209633
1105	12	0.43865801172181307
1105	13	0.5189231468824644
1105	14	1.1828937374952204
1105	15	0.6343056522221318
1105	16	0.9563169513009697
1105	17	1.3951460522173775
1105	18	1.3328619054028152
1105	19	0.9952553770515067
1105	20	0.6943650791701401
1105	21	0.4755287469912517
1105	22	0.9814114571357919
1105	23	2.541564732203568
1105	24	1.3892699865311897
1105	25	1.3803318443643917
1106	1	-0.5249345873704669
1106	2	-0.074606207947852
1106	3	-0.26818164321881355
1106	4	-2.6435064606825343
1106	5	-0.5461433646278238
1106	6	-0.46669333286712344
1106	7	-0.3972526006127879
1106	8	-0.3599542488022749
1106	9	-0.35141586251589274
1106	10	-0.17357318797755994
1106	11	-0.23884779026930403
1106	12	-0.38258489933068063
1106	13	-0.33745599034184437
1106	14	0.40490735202169503
1106	15	-0.14851322586117277
1106	16	-1.3380281998546764
1106	17	-5.747440052040293
1106	18	-7.2220382084632035
1106	19	-3.045443421351134
1106	20	-1.7004593823508927
1106	21	-1.2038968896407312
1106	22	-1.6306077527515583
1106	23	-4.978335632900116
1106	24	-2.1698021629645865
1106	25	-0.9361083819368687
1107	1	0.42258241557992804
1107	2	0.8276628903363132
1107	3	0.48100626851188366
1107	4	0.9138224849191587
1107	5	0.9351835956941059
1107	6	0.4160146240324707
1107	7	0.32823603393372736
1107	8	0.31813520099492365
1107	9	0.253781901648928
1107	10	0.8125358422927036
1107	11	0.42093045851978417
1107	12	0.54290890433969
1107	13	0.4958111880120484
1107	14	1.2366667641872482
1107	15	0.6646242074839179
1107	16	-5.4783980695780485
1107	17	-1.0959094975803154
1107	18	0.5670017570591597
1107	19	0.49594907337520766
1107	20	0.4058447003954342
1107	21	0.4395018555229342
1107	22	0.5411748406230998
1107	23	2.458309714982974
1107	24	1.4487793243856615
1107	25	1.0317791715483295
1108	1	0.39908280638885785
1108	2	0.7094518724224059
1108	3	0.47130047786009754
1108	4	1.0217885130076638
1108	5	0.7529986048534028
1108	6	0.37849131474095543
1108	7	0.3194281892280202
1108	8	0.32202253210596155
1108	9	0.4625054187689912
1108	10	1.0150282023532071
1108	11	0.5328057063078546
1108	12	0.6175537183164863
1108	13	0.5196476553870539
1108	14	1.1224332008216322
1108	15	0.8230970745745623
1108	16	0.9915479735097961
1108	17	1.0763870921775904
1108	18	0.8235788668570478
1108	19	0.8778541780201294
1108	20	0.6482695531715521
1108	21	0.7809312605088863
1108	22	1.1491775552191257
1108	23	2.8168169461299613
1108	24	1.6486750607146945
1108	25	1.6621450357087753
1109	1	0.3316809368008151
1109	2	0.6431180694267766
1109	3	0.5610252467343848
1109	4	0.39641543283686786
1109	5	0.2522203102625937
1109	6	0.18870295387877434
1109	7	0.1905570372146741
1109	8	0.20024879535096574
1109	9	0.23754076644311795
1109	10	0.7168653178927968
1109	11	0.3412215175474813
1109	12	0.40436168137819095
1109	13	0.1763176492554095
1109	14	-0.8980570724947654
1109	15	-0.15991543902483585
1109	16	-0.2770493516496515
1109	17	0.7061206925890744
1109	18	1.5528823090860229
1109	19	0.7331514500632466
1109	20	0.5840166350024703
1109	21	0.2781491622889902
1109	22	0.16727113630290802
1109	23	-4.426513326505827
1109	24	-1.5187234673214363
1109	25	-0.17821918934946268
1110	1	0.40475029013774133
1110	2	0.7551383802890719
1110	3	0.5573878475977914
1110	4	1.094910392179429
1110	5	0.764849634925227
1110	6	0.3821119458343034
1110	7	0.2913020708985741
1110	8	0.2557702934240922
1110	9	0.3377957924723347
1110	10	0.5716838215180431
1110	11	0.3726156148306572
1110	12	0.4930800728087945
1110	13	0.2618157331844486
1110	14	1.0538840689740212
1110	15	0.5735170961031315
1110	16	0.9699139061780926
1110	17	1.239813144820829
1110	18	1.8878681054654862
1110	19	1.0655623807447228
1110	20	0.6079300887882653
1110	21	0.4883378407416501
1110	22	0.7413956714632803
1110	23	2.2915072226088142
1110	24	1.289090609431227
1110	25	1.4659512348927421
1111	1	0.41739245557367255
1111	2	0.7682046096688246
1111	3	0.5954928243256674
1111	4	1.1464398438699916
1111	5	0.7737871951413666
1111	6	0.3097172831301833
1111	7	0.19679975208609335
1111	8	0.023744857888878812
1111	9	0.1265163833512304
1111	10	0.48495439417830255
1111	11	0.24961770115410076
1111	12	0.4131458476860814
1111	13	0.18296345913537237
1111	14	-0.5749763730743496
1111	15	-0.041117982098668904
1111	16	0.378556588278272
1111	17	1.050840032807173
1111	18	1.6649860854703462
1111	19	0.6961858851929463
1111	20	0.4647999592674168
1111	21	0.4076834678774688
1111	22	0.7442319882164199
1111	23	0.7763915814287756
1111	24	0.6680853812198961
1111	25	0.3808738879419309
1112	1	0.40820486827417923
1112	2	0.8045668843675529
1112	3	0.5306721477005567
1112	4	1.08604428218716
1112	5	0.896039838843393
1112	6	0.3164526895358435
1112	7	0.3145230838788393
1112	8	0.3220378826518342
1112	9	0.33711246499271397
1112	10	0.7106866640184464
1112	11	0.2833003574505426
1112	12	0.4304667186937863
1112	13	0.2593343103496011
1112	14	0.7745346905517181
1112	15	0.44339041542696833
1112	16	0.758858743695157
1112	17	0.8502842564470185
1112	18	1.136904312164262
1112	19	0.599301354771967
1112	20	0.40357417667517836
1112	21	0.3876478514596613
1112	22	0.39156166465721043
1112	23	2.0962251583488296
1112	24	1.003949306295965
1112	25	1.1677473790100024
1113	1	0.29715114104699936
1113	2	0.7420795216506519
1113	3	0.39602177499530455
1113	4	0.6022869506224993
1113	5	0.5738022179475237
1113	6	0.1568669440419015
1113	7	0.0581705812019635
1113	8	-0.09758239925126588
1113	9	0.09969357160927217
1113	10	0.42860018987961723
1113	11	0.15845835987301626
1113	12	0.11908881726090925
1113	13	0.008148006622477055
1113	14	0.7115833624775618
1113	15	0.12116987062957207
1113	16	0.5580863219046535
1113	17	1.0702551611037876
1113	18	1.5508047802434106
1113	19	0.6098242093833477
1113	20	0.28938184314482385
1113	21	0.24893268815588954
1113	22	0.5404342928744512
1113	23	0.3253481726217373
1113	24	0.38302666141012054
1113	25	0.8594746108463696
1114	1	-0.3586181066332692
1114	2	-3.2612004477012526
1114	3	-1.138470713430415
1114	4	-0.021594762872105377
1114	5	0.14689052582934892
1114	6	-0.05472677982778862
1114	7	-0.11750415671590986
1114	8	-0.06945610050192386
1114	9	-0.015508147222746516
1114	10	0.05273921584676344
1114	11	-0.10625108158303931
1114	12	-0.0308014330433366
1114	13	-0.14975850215960662
1114	14	0.6923891137363114
1114	15	0.029096547662653904
1114	16	0.4177677705929952
1114	17	0.8350319893700728
1114	18	0.9454795781881202
1114	19	0.41955941458526524
1114	20	0.15757209532251437
1114	21	0.1591670527909841
1114	22	0.24217730768196333
1114	23	1.7927656462414703
1114	24	0.7702639627719634
1114	25	0.7236325510007617
1115	1	0.16466673320599057
1115	2	0.4982214351804437
1115	3	0.24724975355844236
1115	4	0.44680117706071343
1115	5	0.4442365671549311
1115	6	-0.07281024077063591
1115	7	-0.14582417321219054
1115	8	-0.0777334415215023
1115	9	-0.1183739057678892
1115	10	0.1117163152439673
1115	11	0.031467004863905856
1115	12	-0.028951423709720814
1115	13	-0.19818814452619193
1115	14	0.7427258563752375
1115	15	0.06697582211194231
1115	16	0.49375853312886875
1115	17	0.9946381595484013
1115	18	1.3687652255636849
1115	19	0.6610326763070304
1115	20	0.1394671313780691
1115	21	0.18472398053226158
1115	22	0.4270688480443141
1115	23	2.1441115463420957
1115	24	0.9608444085996126
1115	25	0.6827375979524248
1116	1	0.2811768387987499
1116	2	0.20042925509380538
1116	3	0.3052127894257346
1116	4	0.9600079913621542
1116	5	0.32482988871284846
1116	6	0.0791442638865476
1116	7	0.0023894621275246664
1116	8	0.08639165154848172
1116	9	0.10458887045082577
1116	10	0.5302079780685389
1116	11	0.15638533836916935
1116	12	0.24395791402501033
1116	13	0.13606930966864184
1116	14	0.8664132275597183
1116	15	0.1641565824331508
1116	16	0.6318582423926387
1116	17	0.8413209384618483
1116	18	1.4907322494927584
1116	19	0.6797839526201344
1116	20	0.4053464839127656
1116	21	0.42298497015824665
1116	22	0.6419673429948531
1116	23	2.017422865686683
1116	24	0.9744506957442667
1116	25	1.2708378510747025
1201	1	1.1238876264287079
1201	2	1.4872508972272236
1201	3	1.1426495123128042
1201	4	1.8203298395592924
1201	5	1.4278852693086463
1201	6	1.1039654161326382
1201	7	0.9960098461058777
1201	8	1.1076484846990766
1201	9	1.228368817729617
1201	10	1.5264955263768698
1201	11	1.1602243442866964
1201	12	1.3297280480053075
1201	13	1.1547447087712968
1201	14	2.007050353243038
1201	15	1.5778094700467804
1201	16	1.8476069535224084
1201	17	2.308180449663162
1201	18	2.817124540376966
1201	19	2.0034581464444443
1201	20	1.652014666897351
1201	21	1.5355387216842828
1201	22	1.8491857938622687
1201	23	3.398854969636112
1201	24	2.5001674475580202
1201	25	2.600196293077481
1202	1	0.9169691418631629
1202	2	1.2902483790482364
1202	3	1.110348349423404
1202	4	1.742046728335005
1202	5	1.490715249785687
1202	6	1.0656692896012174
1202	7	1.021389695015138
1202	8	0.9950440085651877
1202	9	1.1232826620552316
1202	10	1.6600642670044365
1202	11	1.2053755764967562
1202	12	1.2783685142928292
1202	13	1.3001647353677939
1202	14	2.0331573617029832
1202	15	1.537854740680281
1202	16	1.7903860045367566
1202	17	2.190143497508558
1202	18	2.8814207234966744
1202	19	2.0629103717790187
1202	20	1.7556425422899267
1202	21	1.6759636997563163
1202	22	2.0021909151026804
1202	23	3.6337806975030134
1202	24	2.392911696772856
1202	25	2.581929026574926
1203	1	1.0447012457378122
1203	2	1.330799387816839
1203	3	1.024962106661505
1203	4	1.673640613632358
1203	5	1.4344585244275336
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2211	17	-0.1940423027958147
2211	18	-5.662709495520062
2211	19	-2.1371134487728227
2211	20	-1.2308167973514372
2211	21	-1.0445127112805324
2211	22	-0.6516647058858709
2211	23	-5.110415689713289
2211	24	-2.3840908837869286
2211	25	-1.635756409804351
2212	1	0.23377336560897177
2212	2	0.5205469495995203
2212	3	0.21058192086526972
2212	4	0.7266619575332953
2212	5	0.6084968261935302
2212	6	0.01837120554633742
2212	7	-0.08747862928389338
2212	8	-0.09391294846628284
2212	9	-0.2217153839838133
2212	10	-4.262362579089903
2212	11	-1.3510658219544354
2212	12	-0.4867079560295622
2212	13	-0.19031732142202173
2212	14	0.5346463597064925
2212	15	0.1233155697445909
2212	16	0.39475192426750993
2212	17	0.8299427313431877
2212	18	1.3913541293170866
2212	19	0.5999301958427807
2212	20	0.2583385830599241
2212	21	0.04790715983570237
2212	22	0.3480364750919378
2212	23	1.5567589061860474
2212	24	0.6894493842932903
2212	25	0.6718876876730207
2213	1	-1.4837769899753006
2213	2	-3.8551197864512226
2213	3	-1.6585606401390969
2213	4	-3.166357742538537
2213	5	-1.2050635206976743
2213	6	-1.012126129100686
2213	7	-1.014328685389522
2213	8	-0.9684687602644431
2213	9	-0.77265017445567
2213	10	-0.9403741554566594
2213	11	-0.790070705128123
2213	12	-0.7353246586695334
2213	13	-1.1056271117074132
2213	14	-6.280782285202616
2213	15	-2.770230925525844
2213	16	-2.112909877789768
2213	17	-0.8821210776078772
2213	18	-0.19128026636170148
2213	19	-0.7334391721253084
2213	20	-0.9038603358811415
2213	21	-1.1313415448816144
2213	22	-1.088372223702038
2213	23	-5.776639998911357
2213	24	-3.2106226286202997
2213	25	-2.3381136638744096
2214	1	-0.01307780746358489
2214	2	0.12090623776640186
2214	3	-0.02970774185145686
2214	4	0.2525117281025828
2214	5	-0.019437994056410446
2214	6	-0.22359434455557903
2214	7	-0.35866536327123555
2214	8	-0.35230983647620917
2214	9	-0.2698667648944095
2214	10	0.23161101231595893
2214	11	-0.35696827438102474
2214	12	-0.2605405465656858
2214	13	-0.42697745033255075
2214	14	0.0982495817501956
2214	15	-0.19955690006698035
2214	16	0.1710255277092685
2214	17	0.4383328236385182
2214	18	0.9995366557946141
2214	19	0.12031033975637939
2214	20	-0.07624643210745319
2214	21	-0.26654708206101674
2214	22	0.30991271064326753
2214	23	1.7494233454657007
2214	24	0.8202421962744353
2214	25	0.5810190670164204
2215	1	-2.382598554147279
2215	2	-4.389259555193423
2215	3	-2.4583385233839685
2215	4	-4.055633474814215
2215	5	-5.025472948794718
2215	6	-2.439329384625754
2215	7	-1.8784654897772235
2215	8	-2.1508740382651936
2215	9	-1.6844836143582498
2215	10	-3.2786232176981827
2215	11	-2.305010937332078
2215	12	-1.9585634429560912
2215	13	-1.894671990584726
2215	14	-1.2151048558053787
2215	15	-1.8351210078715638
2215	16	-2.6535300230393233
2215	17	-7.00341773280881
2215	18	-3.2724596101901966
2215	19	-2.376411954238762
2215	20	-2.141523260924558
2215	21	-2.1488566070071613
2215	22	-1.881092310809045
2215	23	-6.427133066060662
2215	24	-3.8639096094195224
2215	25	-3.5186452371111336
2216	1	-1.0062537362325017
2216	2	-0.5367746171540659
2216	3	-0.7932139740652389
2216	4	-3.077615605284123
2216	5	-1.2319105720808459
2216	6	-1.1057523634469462
2216	7	-1.070048562146006
2216	8	-0.9755784727119448
2216	9	-0.9415868399943434
2216	10	-0.630800654088965
2216	11	-1.2236604707360144
2216	12	-1.9431021851307193
2216	13	-1.3062259702822985
2216	14	-6.8771567080589975
2216	15	-3.3363894633229876
2216	16	-1.890007354806393
2216	17	-0.9398013778246934
2216	18	-0.2200308021297417
2216	19	-0.7603683969469301
2216	20	-1.3128043554341673
2216	21	-1.4229195253949598
2216	22	-0.8967717725892754
2216	23	0.29345311219073267
2216	24	-0.3733406412690954
2216	25	-0.2736493870898684
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	85769.0
3	5.0
4	741.0
5	4369.0
6	5204.0
7	4639.0
8	4429.0
9	4251.0
10	3920.0
11	3971.0
12	3852.0
13	3839.0
14	3950.0
15	4235.0
16	4256.0
17	4621.0
18	5167.0
19	6148.0
20	7594.0
21	9472.0
22	11814.0
23	14414.0
24	17354.0
25	20061.0
26	23394.0
27	26847.0
28	29669.0
29	36248.0
30	42685.0
31	48690.0
32	62742.0
33	82939.0
34	108131.0
35	142016.0
36	201330.0
37	281121.0
38	691728.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.26508037541873	18.124469053435806	12.128797745377561	25.48165282576791
2	14.010710825612879	19.427595495246376	42.52410632046137	24.037587358679367
3	19.67095968599744	23.471771147707614	28.69172063399238	28.16554853230257
4	10.629382104603634	16.047329216019733	39.570615906355975	33.752672773020656
5	12.571142138075794	37.61804808599366	36.56620116274039	13.244608613190156
6	32.739773295665294	36.7540350448029	17.889237261927136	12.61695439760468
7	29.14057566762628	30.693953451370554	21.371051545571614	18.794419335431552
8	25.064153911710495	37.601725157233645	19.984496655941612	17.349624275114248
9	26.151761517615174	15.427612441262028	19.713880808532856	38.70674523258994
10	13.616322958262678	29.4926177700643	35.38000208860665	21.511057183066374
11	35.13943933895277	22.422762554114815	23.679752669133812	18.758045437798604
12	22.903072152933433	24.744186139015568	32.004924354672866	20.347817353378137
13	29.135621517830597	21.503511627698852	25.47951144473425	23.8813554097363
14	20.13366056521086	21.88727803207416	28.421938192554347	29.55712321016063
15	23.317616418780943	30.715853001851123	22.913381950856426	23.053148628511508
16	21.974613027418112	28.408071046530186	27.944066917629325	21.67324900842238
17	21.476338203112608	28.426526432404774	28.404797149436305	21.692338215046313
18	21.064404740300258	27.59715900192168	30.398480558255624	20.93995569952244
19	23.197593177353987	27.18973619433601	27.665481488848553	21.947189139461447
20	23.162040006563345	27.419760636843233	28.357224898939425	21.060974457654
21	22.847753249814275	26.91644812281021	27.338220361823097	22.897578265552415
22	22.441949457873356	28.001613226467853	27.6979646194173	21.858472696241492
23	21.115630562986684	27.2956053026762	29.481616659870912	22.107147474466203
24	22.544081606671124	27.240035885928897	28.43119410229479	21.78468840510519
25	22.43375824525548	27.25075273308619	28.164823061106265	22.15066596055206
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	130.0
1	130.0
2	252.5
3	375.0
4	375.0
5	375.0
6	761.0
7	1147.0
8	1147.0
9	1147.0
10	2037.0
11	2927.0
12	2927.0
13	2927.0
14	5463.0
15	7999.0
16	7999.0
17	7999.0
18	14640.5
19	21282.0
20	21282.0
21	21282.0
22	34441.0
23	47600.0
24	47600.0
25	47600.0
26	67953.0
27	88306.0
28	88306.0
29	88306.0
30	115446.5
31	142587.0
32	142587.0
33	142587.0
34	166665.0
35	190743.0
36	190743.0
37	190743.0
38	211425.0
39	232107.0
40	232107.0
41	232107.0
42	245950.5
43	259794.0
44	259794.0
45	259794.0
46	268988.5
47	278183.0
48	278183.0
49	278183.0
50	265592.0
51	253001.0
52	253001.0
53	253001.0
54	223448.0
55	193895.0
56	193895.0
57	193895.0
58	164062.5
59	134230.0
60	134230.0
61	134230.0
62	108061.5
63	81893.0
64	81893.0
65	81893.0
66	62529.5
67	43166.0
68	43166.0
69	43166.0
70	31532.0
71	19898.0
72	19898.0
73	19898.0
74	13903.5
75	7909.0
76	7909.0
77	7909.0
78	5430.0
79	2951.0
80	2951.0
81	2951.0
82	2007.0
83	1063.0
84	1063.0
85	1063.0
86	684.0
87	305.0
88	305.0
89	305.0
90	199.0
91	93.0
92	93.0
93	93.0
94	55.0
95	17.0
96	17.0
97	17.0
98	15.5
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1109556252066126
2	0.04667891221729804
3	0.03360483989232532
4	0.029578224461440185
5	0.03360483989232532
6	0.017945779883327576
7	0.019138851122108357
8	0.021674127504517516
9	0.02808688541296421
10	0.03504646763918543
11	0.018989717217260758
12	0.019238273725340087
13	0.01476425657991216
14	0.01993423194796221
15	0.020182788456041538
16	0.021177014488358858
17	0.02505449601439639
18	0.01739895556555305
19	0.0330580155745508
20	0.022121529219060306
21	0.0286834210323546
22	0.03668694059250901
23	0.027589772396805552
24	0.03971932999107682
25	0.030323893985678176
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2011615.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.56824369448216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.66060209977138	73.13457841868151
2	7.709902409714255	12.57766397232495
3	1.455734765350648	3.562251842839544
4	0.45775934814203234	1.493545042507064
5	0.22360055119202113	0.9119352124925656
6	0.11553130265994384	0.5654211269824365
7	0.07605840997078583	0.4342765643658324
8	0.05280684794028847	0.34458894732247314
9	0.037686695030946335	0.27666337718914746
>10	0.187400141899964	2.7252771843133203
>50	0.01177930606526321	0.6681004213091296
>100	0.009170213137019032	1.4841596106363757
>500	0.0011069488830499363	0.5640232536413557
>1k	8.609602423720676E-4	1.2575150253943175
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	2844	0.14137894179552252	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2743	0.13635810033232004	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2276	0.11314292247771068	No Hit
CCCATGTACTCTGCGTTGATACCAC	2158	0.10727698888703852	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2080	0.10339950736100098	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2050	0.10190816831252501	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.971130161586586E-5	0.0
5	0.0	0.0	0.0	4.971130161586586E-5	0.0
6	0.0	0.0	0.0	4.971130161586586E-5	0.0
7	0.0	0.0	0.0	4.971130161586586E-5	0.0
8	4.971130161586586E-5	0.0	0.0	4.971130161586586E-5	0.0
9	4.971130161586586E-5	0.0	0.0	4.971130161586586E-5	0.0
10	4.971130161586586E-5	0.0	0.0	4.971130161586586E-5	0.0
11	4.971130161586586E-5	0.0	0.0	4.971130161586586E-5	0.0
12	4.971130161586586E-5	0.0	0.0	4.971130161586586E-5	9.942260323173172E-5
13	4.971130161586586E-5	0.0	0.0	4.971130161586586E-5	9.942260323173172E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGAT	40	1.2868377E-5	18.997736	7
TAGGACC	760	0.0	14.251139	4
TCGAGAC	40	0.005281376	14.249366	3
TGTAGGA	1760	0.0	12.685356	2
GTAGGAC	1705	0.0	12.647531	3
GTCCTAC	1675	0.0	12.321931	1
CGAATTG	55	0.0030717244	12.089469	14
TAGGACA	675	0.0	11.963919	4
GGCGAGG	495	0.0	11.89846	19
TGGCGAG	1295	0.0	11.736929	18
CTGTAGG	1700	0.0	11.693141	1
GCGTTTA	65	8.033247E-4	11.690914	13
GGCGAGA	845	0.0	11.466945	19
ACTGCGC	75	2.0766893E-4	11.398642	8
TCCTACA	1910	0.0	11.340939	2
AACCGTG	85	5.3308948E-5	11.175139	7
AGGACGT	1110	0.0	11.127015	5
GATCTCG	60	0.005878621	11.08284	19
TACCGTG	60	0.0058818865	11.082014	7
GGACGTG	1115	0.0	11.075464	6
>>END_MODULE