FastQCFastQC Report
Thu 26 May 2016
SRR1513081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513081_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011615
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC33050.16429585184043666No Hit
GTATCAACGCAGAGTACTTTTTTTT30160.14992928567345143No Hit
CCCATGTACTCTGCGTTGATACCAC29200.14515700071832832No Hit
GTCCTACAGTGGACATTTCTAAATT28370.14103096268421145No Hit
CTGTAGGACGTGGAATATGGCAAGA24960.12407940883320118No Hit
GTCCTACAGTGTGCATTTCTCATTT24960.12407940883320118No Hit
TATCAACGCAGAGTACTTTTTTTTT21270.1057359385369467No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGG350.002139262416.321093
TACACCG701.069324E-412.2408185
TAGGACC7500.012.1864144
GGCGAGG5100.011.71838819
GCCGGTT907.5842327E-611.59438211
GTAGGAC16150.011.5544573
AACCACG752.1004122E-411.3847087
GTCCTAC17250.011.3803621
GATCTCG600.00594007611.06736519
TGTAGGA18350.011.0064582
TCCTACA19150.010.8948972
GGACGTG12600.010.8807276
TAGGACA5950.010.8807274
AGGACGT13150.010.8600425
ATAATAC1951.8189894E-1210.741233
GACGTGA6550.010.7184287
TCCAACG1701.4188117E-1010.60604218
AGGACCT12300.010.6042865
CCTACAG19300.010.507233
TGGCGAG14400.010.47811818
Produced by FastQC (version 0.11.5)