FastQCFastQC Report
Thu 26 May 2016
SRR1513080_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513080_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1820424
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC80030.4396228570926333No Hit
CCCATGTACTCTGCGTTGATACCAC62240.34189837092897035No Hit
GAGTACATGGGAAGCAGTGGTATCA34710.1906698659213458No Hit
GTATCAACGCAGAGTACTTTTTTTT27200.14941574050880455No Hit
GTCCTACAGTGGACATTTCTAAATT24810.13628693095674413No Hit
GTATCAACGCAGAGTACATGGGAAG22040.12107069561816369No Hit
GCGTTGATACCACTGCTTCCCATGT21790.11969738917966363No Hit
CATGTACTCTGCGTTGATACCACTG20290.1114575505486634No Hit
CTGTAGGACGTGGAATATGGCAAGA20080.11030397314032335No Hit
GTCCTACAGTGTGCATTTCTCATTT19410.10662351188514324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6500.013.3007694
GTCCTAC15650.012.2137291
GGCGTGC1251.4133548E-912.1586998
CGTGCGC951.0410859E-611.99904610
CCAACGA1302.6175258E-911.6920219
GACCTCG658.0317876E-411.6910567
TCCAACG1657.2759576E-1211.51486818
TCCTACA17250.011.5104932
AGCACCG1001.9292802E-611.4003475
AGGACCT10500.011.4003475
AAGGCGT1251.8335413E-811.3994066
GCGTGCG1001.9318322E-611.3990949
GTAGGAC14050.011.3597763
CCTACAC2450.011.245243
AGTACCG600.005874982611.0836715
CTGTAGG14900.010.9776341
CCTACAG17650.010.9266783
AGGACGT9900.010.8437635
GGACCTG10000.010.8294366
ATTTCTA10200.010.80276515
Produced by FastQC (version 0.11.5)