##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513080_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1820424
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.608343990191297	31.0	30.0	34.0	25.0	34.0
2	29.336045338888084	31.0	30.0	34.0	22.0	34.0
3	29.68062989721076	31.0	30.0	34.0	25.0	34.0
4	32.55041188206703	35.0	33.0	37.0	22.0	37.0
5	32.80875993724539	35.0	33.0	37.0	25.0	37.0
6	33.29847332269845	37.0	35.0	37.0	28.0	37.0
7	33.31741011984021	37.0	35.0	37.0	28.0	37.0
8	33.30183023295672	37.0	35.0	37.0	28.0	37.0
9	34.714460477339344	39.0	35.0	39.0	27.0	39.0
10	34.22811114333803	39.0	35.0	39.0	25.0	39.0
11	34.604775041418925	39.0	35.0	39.0	27.0	39.0
12	34.43268436364276	39.0	35.0	39.0	27.0	39.0
13	34.53168327818135	39.0	35.0	39.0	27.0	39.0
14	34.8702928548514	40.0	34.0	41.0	18.0	41.0
15	35.43069416795208	40.0	35.0	41.0	27.0	41.0
16	35.02814454215062	40.0	34.0	41.0	22.0	41.0
17	34.582281929924015	39.0	34.0	41.0	17.0	41.0
18	34.00864908394967	39.0	33.0	41.0	16.0	41.0
19	34.898690634709276	40.0	34.0	41.0	22.0	41.0
20	35.15937550812338	40.0	35.0	41.0	25.0	41.0
21	35.1826453617399	40.0	36.0	41.0	25.0	41.0
22	34.78383222809631	40.0	35.0	41.0	18.0	41.0
23	33.138930820512144	39.0	32.0	41.0	10.0	41.0
24	34.25406553638054	39.0	34.0	41.0	17.0	41.0
25	33.987285928992364	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7586266667684356
1101	2	1.0494690137456608
1101	3	0.7892231668876519
1101	4	0.9760370076943516
1101	5	1.0788027229105737
1101	6	0.7702978229260324
1101	7	0.8693466159878085
1101	8	0.8271978264834914
1101	9	0.9177969227077156
1101	10	1.2724674996715208
1101	11	0.9231871478623717
1101	12	1.0107101231773683
1101	13	0.8720656998993377
1101	14	-0.16790861189232942
1101	15	0.486744046196236
1101	16	1.0735719444755745
1101	17	1.6975254853080344
1101	18	-4.135075587919967
1101	19	-0.637898887097947
1101	20	0.22784477295604688
1101	21	0.807334882025522
1101	22	1.2554044363210437
1101	23	2.995528001775874
1101	24	1.9394329867447198
1101	25	2.1557761171014036
1102	1	1.0621454828927135
1102	2	1.4487536438961008
1102	3	1.2328700544628255
1102	4	1.7304747878954956
1102	5	1.5019805784910005
1102	6	1.097686348310738
1102	7	1.0717650348083936
1102	8	1.0175799522814515
1102	9	1.2493525930540699
1102	10	1.5985969135369729
1102	11	1.2160642993925919
1102	12	1.4017983717887148
1102	13	1.323675003055449
1102	14	2.174961126065803
1102	15	1.6795527218293387
1102	16	1.9484005554432002
1102	17	1.6785654739565032
1102	18	2.08280411451679
1102	19	1.789078224893224
1102	20	1.5889853789238941
1102	21	1.627953939210471
1102	22	1.9789746532118713
1102	23	3.6175976479013627
1102	24	2.506642199343645
1102	25	2.8307838495368145
1103	1	0.6721998470799697
1103	2	0.9614397015986533
1103	3	0.8290775162363317
1103	4	1.2358608559328346
1103	5	1.1278934907232028
1103	6	0.6701911408890595
1103	7	0.5874726631163085
1103	8	0.5597582805247967
1103	9	0.6435876311254063
1103	10	0.978061534205743
1103	11	0.6370266208014783
1103	12	0.8566443906504375
1103	13	0.7407017664642481
1103	14	1.53299130657269
1103	15	0.9957740991833361
1103	16	1.343422413993629
1103	17	1.6844991801628169
1103	18	1.7397031446474074
1103	19	1.1985713079260734
1103	20	1.1442014257213131
1103	21	1.0314521806415584
1103	22	1.3434752610591403
1103	23	2.8462851807930676
1103	24	1.711313049418088
1103	25	1.7676292657782184
1104	1	0.6763380589828749
1104	2	1.0719039458541886
1104	3	0.8253069833673372
1104	4	1.3636741083142851
1104	5	1.1362594907242425
1104	6	0.8502632497262255
1104	7	0.7961750425621119
1104	8	0.768887709273443
1104	9	0.943347207575826
1104	10	1.3557035762721839
1104	11	0.8186879259162296
1104	12	0.9232763205018628
1104	13	0.8357669774135843
1104	14	1.8002382394993859
1104	15	1.260229978480453
1104	16	1.4772250328474357
1104	17	2.024193595139124
1104	18	2.585792883329688
1104	19	1.7814344964324107
1104	20	1.1459389174732735
1104	21	1.2215309139508292
1104	22	1.5516013843420282
1104	23	3.1122141197890727
1104	24	2.0683922769588037
1104	25	2.1982600540539394
1105	1	0.7461799461495495
1105	2	1.1075007696129155
1105	3	0.7650981431557717
1105	4	0.8653867123758445
1105	5	0.94223173485058
1105	6	0.7067770911703732
1105	7	0.5874856503974684
1105	8	0.47754060875728754
1105	9	0.7243695589600208
1105	10	1.3156639600220643
1105	11	0.8022271749427929
1105	12	0.795303123993115
1105	13	0.875782477871148
1105	14	1.73913673217303
1105	15	0.9690348457364095
1105	16	1.4519233557663185
1105	17	1.8930147845759464
1105	18	1.9367030567235162
1105	19	1.4521508298928296
1105	20	1.2792029147131032
1105	21	0.9646197616344665
1105	22	1.4760559208415955
1105	23	2.917754448794838
1105	24	1.9804200348740153
1105	25	1.8533715557813437
1106	1	-0.4429790828357305
1106	2	0.06176661924303417
1106	3	-0.13957061062729537
1106	4	-2.689094279809172
1106	5	-0.5055458947977627
1106	6	-0.4457501614483377
1106	7	-0.3672700177192141
1106	8	-0.30381466003060353
1106	9	-0.20839561251459315
1106	10	0.061163558714866895
1106	11	-0.07417382812477769
1106	12	-0.1298884812835297
1106	13	-0.18924454684231762
1106	14	0.45186659482084224
1106	15	-0.014655414155740232
1106	16	-0.9802874079044059
1106	17	-5.698600086716745
1106	18	-6.79903831808112
1106	19	-2.773355627293249
1106	20	-1.438690214082655
1106	21	-0.9515383907722779
1106	22	-1.1317779163832355
1106	23	-4.829089305961272
1106	24	-1.9719593411815382
1106	25	-0.5871315969402531
1107	1	0.7049366746016261
1107	2	1.0790025244655297
1107	3	0.7661341648526836
1107	4	1.1273579778494849
1107	5	1.1158912213833077
1107	6	0.6431667496131723
1107	7	0.5504088421887943
1107	8	0.6468469078450596
1107	9	0.7024515370370708
1107	10	1.0992488251799557
1107	11	0.8138470527036148
1107	12	0.8190730900085725
1107	13	0.7498223404968201
1107	14	1.5955730754348991
1107	15	1.0985048147606875
1107	16	-5.459189648853364
1107	17	-0.7991358245340123
1107	18	0.9641279859213228
1107	19	0.8818933882987849
1107	20	0.8787025901898957
1107	21	0.7599448575312593
1107	22	1.0674664097047781
1107	23	2.851515937004919
1107	24	1.949467448637698
1107	25	1.6545330125884092
1108	1	0.538188508404275
1108	2	0.7050747974402434
1108	3	0.45650021236733096
1108	4	1.0319939189402376
1108	5	0.8273548172622824
1108	6	0.5012829777204999
1108	7	0.39055379167467663
1108	8	0.4051182041907566
1108	9	0.515307839373861
1108	10	0.963897994984606
1108	11	0.597446582720174
1108	12	0.6308364811104923
1108	13	0.44981030519900855
1108	14	1.1554763682772062
1108	15	0.7845896084906059
1108	16	1.0248809012532973
1108	17	1.126692986502455
1108	18	0.9563248904730699
1108	19	0.888825820973814
1108	20	0.7094562147487764
1108	21	0.694195216504248
1108	22	1.0386674085333567
1108	23	2.705125123072392
1108	24	1.3361103666613872
1108	25	1.5729577134695418
1109	1	0.020342885736535976
1109	2	0.32739669289193785
1109	3	0.21915790451140538
1109	4	0.21243146581761607
1109	5	-0.08500630428868305
1109	6	-0.11104573044091381
1109	7	-0.10900304965243635
1109	8	-0.1404815871990266
1109	9	-0.18405012955771838
1109	10	0.39636188065858136
1109	11	-0.07230025528026118
1109	12	0.11596428184758167
1109	13	-0.0991768924532721
1109	14	-1.24878309996501
1109	15	-0.35533396216738566
1109	16	-0.6519779338549085
1109	17	0.38560117855686826
1109	18	1.1244722036189145
1109	19	0.33104710315615193
1109	20	0.1661556030856559
1109	21	0.1170772625106764
1109	22	0.20285333615299095
1109	23	-4.170551144089728
1109	24	-1.5493933189656275
1109	25	-0.30352154741984805
1110	1	0.8143244519553825
1110	2	1.1625955111005482
1110	3	0.9027802968921534
1110	4	1.518322462310124
1110	5	1.1308791228674266
1110	6	0.8084572188342989
1110	7	0.702711753800429
1110	8	0.623358593292032
1110	9	0.7419069570463819
1110	10	1.0554802947407609
1110	11	0.6792746524167725
1110	12	0.87426916145062
1110	13	0.8419618697240665
1110	14	1.6290085926516937
1110	15	1.1299064393586278
1110	16	1.4106999338421957
1110	17	1.6731193639889597
1110	18	2.3500161260208756
1110	19	1.558043166667396
1110	20	1.1486986901483789
1110	21	1.0209247324783135
1110	22	1.228840063037211
1110	23	2.7127101045557396
1110	24	1.7869971707282843
1110	25	2.156858359922751
1111	1	0.4286164232816603
1111	2	0.7952066858050166
1111	3	0.5781294052416044
1111	4	1.0672883951479477
1111	5	0.782192340023748
1111	6	0.3176363041906214
1111	7	0.16626848346508893
1111	8	-0.04643209457014308
1111	9	0.07897068326596468
1111	10	0.390262813251141
1111	11	0.21552248658234419
1111	12	0.3161777982785381
1111	13	0.1670247360245014
1111	14	-0.418679391464984
1111	15	-0.03766556837872059
1111	16	0.4848277939396297
1111	17	0.9891421683924833
1111	18	1.6394482208843968
1111	19	0.6425591186351838
1111	20	0.45643895763694786
1111	21	0.44449977798916507
1111	22	0.7210482147877642
1111	23	0.692080318093133
1111	24	0.47296277947778265
1111	25	0.1258077490288514
1112	1	0.2937090798526505
1112	2	0.6195537413499004
1112	3	0.42254950107200173
1112	4	0.9869589649090855
1112	5	0.8286005814236646
1112	6	0.15599654013615805
1112	7	0.17389626496911603
1112	8	0.1069621497239055
1112	9	0.15093933340666155
1112	10	0.5283167162721938
1112	11	0.2174074932008878
1112	12	0.2877639817551412
1112	13	0.1730270848110962
1112	14	0.6215334877108702
1112	15	0.4087492695322581
1112	16	0.5348370873997155
1112	17	0.8057185577171424
1112	18	0.8861888121342076
1112	19	0.3106298260895599
1112	20	0.2627371730425594
1112	21	0.39565416147198107
1112	22	0.391849687310021
1112	23	1.955044873515078
1112	24	0.9702805356781141
1112	25	1.027202887304938
1113	1	0.3711997661836399
1113	2	0.7735276359108312
1113	3	0.4977615351660738
1113	4	0.495007197396248
1113	5	0.7704479946264513
1113	6	0.32941028905069203
1113	7	0.2933588824262614
1113	8	0.21997346476619128
1113	9	0.33330469620650405
1113	10	0.6628540351158279
1113	11	0.32378085774683285
1113	12	0.3191539970897921
1113	13	0.30351271173788064
1113	14	0.8055259908747558
1113	15	0.42238090256480376
1113	16	0.7941717162627668
1113	17	1.2489179400220607
1113	18	1.7483843322055534
1113	19	0.91861944124323
1113	20	0.6216010131500269
1113	21	0.6522304033491579
1113	22	0.9098759810364925
1113	23	0.7825590957710205
1113	24	0.8551012223744436
1113	25	1.206598039794315
1114	1	-0.397915016164454
1114	2	-3.0596709270255715
1114	3	-1.1441619718977236
1114	4	0.08730733061336338
1114	5	0.17280866944695816
1114	6	-0.06610267905943346
1114	7	-0.17558019587214346
1114	8	-0.10558521563517331
1114	9	-0.04398731423888336
1114	10	-0.005642996280649015
1114	11	-0.16790837725070418
1114	12	-0.11711034110989971
1114	13	-0.2312888879638919
1114	14	0.5416658834604604
1114	15	-0.114945472047566
1114	16	0.21080069290317738
1114	17	0.6769238224072467
1114	18	0.7918523537030282
1114	19	0.2416522046119951
1114	20	-0.001993491507093381
1114	21	-0.024193640055131027
1114	22	0.13356994167805425
1114	23	1.7399003819283223
1114	24	0.60621844612254
1114	25	0.7296723307192892
1115	1	0.06553368896583933
1115	2	0.22930908696141472
1115	3	-0.0030552468744247108
1115	4	0.15294534839579654
1115	5	0.22856927013629047
1115	6	-0.2407359230215107
1115	7	-0.34674844039763286
1115	8	-0.4702702579628095
1115	9	-0.40767386079392054
1115	10	-0.1943546729129011
1115	11	-0.20905395999125886
1115	12	-0.4885724555063007
1115	13	-0.5004892506955372
1115	14	0.30171344280232404
1115	15	-0.40789196912943027
1115	16	-0.1306061621526169
1115	17	0.43051581369310554
1115	18	0.9358434481760227
1115	19	0.19271406216291354
1115	20	-0.3054358202156564
1115	21	-0.2708824617317518
1115	22	0.06600714778582528
1115	23	1.7338747528268001
1115	24	0.437331136185513
1115	25	0.09974066485451516
1116	1	-0.006296311408714672
1116	2	-0.013556945461296266
1116	3	-0.04604462657975361
1116	4	0.6960662474604362
1116	5	-0.025996877717005873
1116	6	-0.06067315125739725
1116	7	-0.21849748613742292
1116	8	-0.16709685330540935
1116	9	-0.23385762015350053
1116	10	0.18772566541107238
1116	11	-0.2507983748875944
1116	12	-0.2002262625022837
1116	13	-0.30950611581882015
1116	14	0.2986974357470942
1116	15	-0.2274550694791273
1116	16	0.12349349632267348
1116	17	0.3356209781050694
1116	18	0.9147122454846865
1116	19	0.03440178327717547
1116	20	-0.192797788732733
1116	21	-0.39070213123934394
1116	22	0.04553779818098036
1116	23	1.387358696398607
1116	24	0.4534288503875601
1116	25	0.684222487091418
1201	1	0.7916730937320864
1201	2	1.1616221109426021
1201	3	0.8958002928872304
1201	4	1.5363805885381225
1201	5	1.269341282648213
1201	6	0.8536905546313349
1201	7	0.7554977428442413
1201	8	0.8339205804366827
1201	9	1.0059299707113922
1201	10	1.4530165309207774
1201	11	1.0964823738099696
1201	12	1.1279646842111646
1201	13	0.9712666898540832
1201	14	1.8197688764237299
1201	15	1.3006876848888496
1201	16	1.648266008879176
1201	17	2.0612907657770947
1201	18	2.541255343381266
1201	19	1.7011649619383036
1201	20	1.4760312876498674
1201	21	1.3939821551917007
1201	22	1.5973789415067827
1201	23	3.1879480083436746
1201	24	2.1530080565004255
1201	25	2.3255672468720334
1202	1	1.0534618765545574
1202	2	1.4247049230352928
1202	3	1.1574919065260403
1202	4	1.7644756128428014
1202	5	1.6026241503998548
1202	6	1.1689171583976972
1202	7	1.014843670368144
1202	8	0.9330897150996833
1202	9	1.1984846091085188
1202	10	1.638389320631525
1202	11	1.2636023590336478
1202	12	1.370121713465693
1202	13	1.3213668456826255
1202	14	2.131006313590639
1202	15	1.6396143480302712
1202	16	1.8671762055600638
1202	17	2.279573491766321
1202	18	2.890037659360587
1202	19	2.1481638517872597
1202	20	1.7828922578167266
1202	21	1.7285344158341402
1202	22	2.115991244446711
1202	23	3.6983737387435056
1202	24	2.4431994192335367
1202	25	2.583578405637944
1203	1	0.9917194794452868
1203	2	1.327213453636638
1203	3	0.9952191891085747
1203	4	1.7273192966573987
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2211	18	-5.4783766162754794
2211	19	-1.8168908776331492
2211	20	-1.033426148777174
2211	21	-0.6665354671936896
2211	22	-0.45259832492813246
2211	23	-4.920002387316682
2211	24	-2.1106545688026515
2211	25	-1.4212744276772966
2212	1	0.3024541337587614
2212	2	0.5396258786542667
2212	3	0.2545515165878989
2212	4	0.7696254074148285
2212	5	0.576972938806712
2212	6	0.18386351125587197
2212	7	9.209659087332511E-4
2212	8	-0.14484049783884245
2212	9	-0.16329285624700418
2212	10	-3.382553832484863
2212	11	-0.9240097883986209
2212	12	-0.25196503957227634
2212	13	-0.03696000902706231
2212	14	0.6847018187965546
2212	15	0.3055770301235583
2212	16	0.45103157888654977
2212	17	0.9699449380278864
2212	18	1.498974487478172
2212	19	0.701778923781454
2212	20	0.2629186999118005
2212	21	0.17840176569171007
2212	22	0.31261967042306793
2212	23	1.7154560976970146
2212	24	0.9270980351098643
2212	25	0.8043955036180108
2213	1	-1.5241680446126438
2213	2	-4.038361929080189
2213	3	-2.026356910061228
2213	4	-3.5927163517466347
2213	5	-1.5627858430678359
2213	6	-1.2256468518800787
2213	7	-1.1441805646682823
2213	8	-1.0023779847331014
2213	9	-0.8728659530950083
2213	10	-1.0963470874759125
2213	11	-1.1208258231160997
2213	12	-0.9042717712038311
2213	13	-1.295094021565113
2213	14	-6.121581162885104
2213	15	-3.0732459714648215
2213	16	-2.1509326769027908
2213	17	-0.9466836972313573
2213	18	-0.12829936811451859
2213	19	-0.8535861125331081
2213	20	-1.0311357958352048
2213	21	-1.2082228660073113
2213	22	-1.0726401770552911
2213	23	-5.7364335682399314
2213	24	-3.3252520643996135
2213	25	-2.434122271175209
2214	1	-0.39328214202073397
2214	2	-0.22336767953503767
2214	3	-0.34493234289097074
2214	4	-0.015637189376320748
2214	5	-0.1768193181856148
2214	6	-0.5016132994806881
2214	7	-0.6949048222202734
2214	8	-0.7090189579943598
2214	9	-0.5307837943056413
2214	10	-0.18942939918920843
2214	11	-0.6389593690285267
2214	12	-0.4779375450726704
2214	13	-0.6040193223252643
2214	14	-0.09628431365661783
2214	15	-0.2791074542367582
2214	16	0.07606837248788878
2214	17	0.13332841958941088
2214	18	0.6680017463566514
2214	19	-0.23900546014980506
2214	20	-0.2710363180013289
2214	21	-0.5629926756861039
2214	22	-0.0397168031834525
2214	23	1.434868863531527
2214	24	0.34617413505205974
2214	25	0.2822518533470202
2215	1	-2.334047147396607
2215	2	-4.452460547394423
2215	3	-2.4500083892298683
2215	4	-3.890371067047056
2215	5	-4.891092611019847
2215	6	-2.2489088837806754
2215	7	-1.6931347250023059
2215	8	-1.9940576395088137
2215	9	-1.7065247674034438
2215	10	-2.961110376450211
2215	11	-2.0428896745741056
2215	12	-1.7333080177705966
2215	13	-1.7846699831521349
2215	14	-1.1127591641990193
2215	15	-1.7080340652485404
2215	16	-2.5406519285202265
2215	17	-6.614375231197943
2215	18	-2.9932221116366655
2215	19	-2.2315978492179624
2215	20	-1.9316941519337547
2215	21	-1.8637693362278185
2215	22	-1.5898992006492563
2215	23	-6.3954441106874675
2215	24	-3.6794424676570543
2215	25	-3.3744657475702873
2216	1	-1.1316773129382511
2216	2	-0.6855557555933665
2216	3	-0.8016563453038259
2216	4	-3.4842468877555177
2216	5	-1.436434527039495
2216	6	-1.120809830370419
2216	7	-1.2086261713374569
2216	8	-1.0696365698595258
2216	9	-1.0738291927991597
2216	10	-0.8717763355255741
2216	11	-1.2126378885823428
2216	12	-2.09824067039343
2216	13	-1.3808176788848883
2216	14	-7.04642429011864
2216	15	-3.428344438394319
2216	16	-1.9440162461307366
2216	17	-0.9371828776570439
2216	18	-0.3129527370381098
2216	19	-0.8859301259568753
2216	20	-1.3451176829229894
2216	21	-1.5956730667484251
2216	22	-1.0093888040946055
2216	23	0.052310591562395814
2216	24	-0.6595064767667083
2216	25	-0.6149937529668961
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	71769.0
3	2.0
4	612.0
5	3732.0
6	4685.0
7	4210.0
8	3896.0
9	3688.0
10	3637.0
11	3432.0
12	3448.0
13	3438.0
14	3448.0
15	3573.0
16	3662.0
17	3898.0
18	4300.0
19	5303.0
20	6433.0
21	8164.0
22	10037.0
23	12357.0
24	14805.0
25	17497.0
26	19847.0
27	22973.0
28	25591.0
29	31032.0
30	37154.0
31	42168.0
32	54265.0
33	71830.0
34	94390.0
35	126182.0
36	180157.0
37	256925.0
38	657884.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.35086021298417	18.23338071814105	12.223960581705798	25.19179848716898
2	13.50898164101465	19.825835579774065	42.97338070781432	23.69180207139697
3	19.644237823223456	24.45048921420741	28.7950301002058	27.110242862363336
4	10.35403822272043	16.446374995878806	39.788005670765884	33.411581110634884
5	11.946494160781288	38.3045274002563	36.79119929837056	12.957779140591857
6	32.13176254435288	36.84039334923755	18.298057978244696	12.729786128164866
7	28.40652539052949	31.133405017172805	21.75448852738061	18.705581064917098
8	24.988914682893995	37.5176032637811	20.351323507191033	17.142158546133874
9	26.6002470541431	15.362085980996174	19.725481964388155	38.31218500047257
10	13.587469624214021	29.823498960821144	35.52947564057051	21.059555774394326
11	34.561452380442184	22.855365795631805	23.73788405505657	18.845297768869447
12	22.891148557359735	25.209350829738614	32.180550770838515	19.71894984206313
13	28.98960397073157	21.738096101461554	25.51988387566081	23.75241605214607
14	20.046043956043956	22.38467032967033	28.824450549450546	28.744835164835163
15	23.783210577934796	30.45112265558251	23.158435507399663	22.60723125908303
16	21.346110629731115	28.619356384261753	28.86875212568798	21.165780860319153
17	21.512875713348475	28.536136945324508	28.566412701971167	21.384574639355847
18	20.946604528906477	27.644142278035343	30.783465436511904	20.625787756546277
19	22.979710418886462	27.38013661933365	28.240890075343405	21.399262886436485
20	22.934842212725183	27.618185234799025	28.633854620540205	20.81311793193559
21	22.480940904440217	27.01984651617207	28.067506069128168	22.43170651025955
22	22.081259280345286	28.31567297134812	27.676283971146987	21.926783777159606
23	20.479156420943312	27.695900520093524	29.760998755402184	22.06394430356098
24	22.05997517109031	28.00506470189646	28.449545050622287	21.485415076390936
25	22.053055388568406	27.411566109875956	28.17691064130025	22.358467860255384
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	188.0
1	188.0
2	332.0
3	476.0
4	476.0
5	476.0
6	968.5
7	1461.0
8	1461.0
9	1461.0
10	2392.0
11	3323.0
12	3323.0
13	3323.0
14	5745.0
15	8167.0
16	8167.0
17	8167.0
18	14479.5
19	20792.0
20	20792.0
21	20792.0
22	33585.0
23	46378.0
24	46378.0
25	46378.0
26	65849.0
27	85320.0
28	85320.0
29	85320.0
30	110224.0
31	135128.0
32	135128.0
33	135128.0
34	155586.5
35	176045.0
36	176045.0
37	176045.0
38	193303.5
39	210562.0
40	210562.0
41	210562.0
42	223041.0
43	235520.0
44	235520.0
45	235520.0
46	250405.0
47	265290.0
48	265290.0
49	265290.0
50	247708.5
51	230127.0
52	230127.0
53	230127.0
54	198270.5
55	166414.0
56	166414.0
57	166414.0
58	138876.5
59	111339.0
60	111339.0
61	111339.0
62	88538.5
63	65738.0
64	65738.0
65	65738.0
66	49695.0
67	33652.0
68	33652.0
69	33652.0
70	24289.5
71	14927.0
72	14927.0
73	14927.0
74	10479.5
75	6032.0
76	6032.0
77	6032.0
78	4187.5
79	2343.0
80	2343.0
81	2343.0
82	1562.0
83	781.0
84	781.0
85	781.0
86	543.5
87	306.0
88	306.0
89	306.0
90	197.0
91	88.0
92	88.0
93	88.0
94	52.0
95	16.0
96	16.0
97	16.0
98	13.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10871093767166332
2	0.04828545437766147
3	0.03674968029426112
4	0.03098179325256094
5	0.0362552899764011
6	0.019885477229480603
7	0.02169824172830066
8	0.02466458363546075
9	0.031860709373200964
10	0.0394413609137212
11	0.022247564303700677
12	0.021588377213220655
13	0.01680927080724051
14	0.023291277196960707
15	0.02246729333386068
16	0.023346209454500708
17	0.026697077164440808
18	0.020984122380280638
19	0.034222796447421044
20	0.024280057832680738
21	0.030652199707320936
22	0.0394413609137212
23	0.030707131964860936
24	0.04235277056334129
25	0.03241003194860099
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1820424.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.20132331361346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.61486727014382	67.63983695201703
2	9.02930086552321	13.941479508302942
3	1.855908331598501	4.298357374444945
4	0.6241671582022432	1.9274612232844281
5	0.2805500804335423	1.082941873260508
6	0.1508755336236546	0.6988674510836231
7	0.08829756504417062	0.4771682206745882
8	0.06106106841239913	0.37711962275042366
9	0.04782350385189919	0.33228340045742777
>10	0.21732012691953387	2.9776824827207737
>50	0.015476868942354541	0.833586190037681
>100	0.010962772742242935	1.7264874240245673
>500	0.0018025822140456853	0.9066108536265431
>1k	0.0014420657712365466	1.9881758371080744
>5k	1.4420657712365466E-4	0.7919415862064725
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	8003	0.4396228570926333	No Hit
CCCATGTACTCTGCGTTGATACCAC	6224	0.34189837092897035	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3471	0.1906698659213458	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2720	0.14941574050880455	No Hit
GTCCTACAGTGGACATTTCTAAATT	2481	0.13628693095674413	No Hit
GTATCAACGCAGAGTACATGGGAAG	2204	0.12107069561816369	No Hit
GCGTTGATACCACTGCTTCCCATGT	2179	0.11969738917966363	No Hit
CATGTACTCTGCGTTGATACCACTG	2029	0.1114575505486634	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2008	0.11030397314032335	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1941	0.10662351188514324	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.0986451508000334E-4	0.0	0.0	0.0
6	0.0	1.0986451508000334E-4	0.0	0.0	0.0
7	0.0	1.0986451508000334E-4	0.0	5.493225754000167E-5	0.0
8	0.0	1.0986451508000334E-4	0.0	5.493225754000167E-5	0.0
9	0.0	1.0986451508000334E-4	0.0	1.0986451508000334E-4	0.0
10	0.0	1.0986451508000334E-4	0.0	1.0986451508000334E-4	0.0
11	0.0	1.0986451508000334E-4	0.0	1.0986451508000334E-4	0.0
12	0.0	1.0986451508000334E-4	0.0	2.1972903016000668E-4	0.0
13	0.0	1.0986451508000334E-4	0.0	2.1972903016000668E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	650	0.0	13.300769	4
GTCCTAC	1565	0.0	12.213729	1
GGCGTGC	125	1.4133548E-9	12.158699	8
CGTGCGC	95	1.0410859E-6	11.999046	10
CCAACGA	130	2.6175258E-9	11.69202	19
GACCTCG	65	8.0317876E-4	11.691056	7
TCCAACG	165	7.2759576E-12	11.514868	18
TCCTACA	1725	0.0	11.510493	2
AGCACCG	100	1.9292802E-6	11.400347	5
AGGACCT	1050	0.0	11.400347	5
AAGGCGT	125	1.8335413E-8	11.399406	6
GCGTGCG	100	1.9318322E-6	11.399094	9
GTAGGAC	1405	0.0	11.359776	3
CCTACAC	245	0.0	11.24524	3
AGTACCG	60	0.0058749826	11.083671	5
CTGTAGG	1490	0.0	10.977634	1
CCTACAG	1765	0.0	10.926678	3
AGGACGT	990	0.0	10.843763	5
GGACCTG	1000	0.0	10.829436	6
ATTTCTA	1020	0.0	10.802765	15
>>END_MODULE