FastQCFastQC Report
Thu 26 May 2016
SRR1513080_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513080_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1820424
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC92990.5108150628644755No Hit
CCCATGTACTCTGCGTTGATACCAC82720.4543996343708938No Hit
GTATCAACGCAGAGTACTTTTTTTT41190.2262659688072669No Hit
GAGTACATGGGAAGCAGTGGTATCA36950.20297469161030618No Hit
TATCAACGCAGAGTACTTTTTTTTT27550.1513383695227046No Hit
GTCCTACAGTGGACATTTCTAAATT26290.1444169050726644No Hit
GGTATCAACGCAGAGTACTTTTTTT24630.1352981503210241No Hit
CATGTACTCTGCGTTGATACCACTG24240.13315579227696406No Hit
GTATCAACGCAGAGTACATGGGAAG23950.131562756808304No Hit
GCGTTGATACCACTGCTTCCCATGT22250.12222427302650371No Hit
CTGTAGGACGTGGAATATGGCAAGA21290.11695077630266355No Hit
ACGCAGAGTACATGGGAAGCAGTGG20670.11354497633518346No Hit
TATCAACGCAGAGTACATGGGAAGC19850.10904053121690331No Hit
GTCCTACAGTGTGCATTTCTCATTT19680.10810668283872328No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC307.764198E-418.98512817
CGTGCGC951.8189894E-1115.97649810
AAGGCGT1500.015.2308086
AAAGGCG1650.015.0000395
AGGCGTG1800.014.2314137
GCGTGCG1152.5465852E-1114.0247759
GGCGTGC1257.2759576E-1213.6621568
TAGGACC5250.013.5989364
GCGCCAC1251.03682396E-1012.91024213
TCCAACG1352.7284841E-1112.6539718
GTCCTAC15900.012.0434041
CGCCACC1353.765308E-1011.95392814
TGTAGGA14750.011.8830572
GTAGGAC14050.011.6534653
TCCTACA18350.011.2648662
CCAACGA1105.052116E-711.21293619
TGGCGAG11500.011.05846918
GGCGAGA8350.011.02183619
TGGACCG700.001466626410.8791495
CCTATAC1855.456968E-1210.8056413
Produced by FastQC (version 0.11.5)