FastQCFastQC Report
Thu 26 May 2016
SRR1513079_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513079_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761610
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC62240.3533131623912217No Hit
CCCATGTACTCTGCGTTGATACCAC47780.27122915968914796No Hit
GAGTACATGGGAAGCAGTGGTATCA25520.1448674791809765No Hit
GTATCAACGCAGAGTACTTTTTTTT21900.12431809537865929No Hit
GTCCTACAGTGGACATTTCTAAATT17950.10189542520762257No Hit
GTCCTACAGTGTGCATTTCTCATTT17700.10047626886768354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAT250.006039684618.99602311
TAGGACC6500.013.8888094
TCCAACG1600.013.65455518
ATACCGA604.09679E-412.6650956
GTAGGAC12200.012.3841843
AGGACCT8600.012.2632425
TGTAGGA13400.011.9846222
CTGTAGG13050.011.953171
GGGCGAA802.8730128E-511.87251611
TAGGACG7950.011.7142294
GGACCTG8500.011.62208756
CGGTTTC907.4770633E-611.60901213
GCGTTTA907.4770633E-611.60901213
GCCGGTT907.479426E-611.60868211
AGGACGT8250.011.5166665
TGGCGAG10400.011.41685318
GGACGTG8550.011.220836
GTCCTAC12250.011.1808841
GTCTAGG1803.6379788E-1211.0967691
ACGACAG600.00587043111.0847925
Produced by FastQC (version 0.11.5)