##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513079_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1761610
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.400406446375758	31.0	30.0	34.0	25.0	34.0
2	29.132871066808203	31.0	30.0	34.0	18.0	34.0
3	29.471042966377347	31.0	30.0	34.0	24.0	34.0
4	32.337899989214414	35.0	33.0	37.0	19.0	37.0
5	32.57742065496903	35.0	33.0	37.0	24.0	37.0
6	33.067963397119684	37.0	35.0	37.0	27.0	37.0
7	33.08199090604617	37.0	35.0	37.0	27.0	37.0
8	33.072700540982396	37.0	35.0	37.0	27.0	37.0
9	34.46761371699752	39.0	35.0	39.0	27.0	39.0
10	33.9734895919074	39.0	34.0	39.0	23.0	39.0
11	34.34952912392641	39.0	35.0	39.0	27.0	39.0
12	34.1802067426956	39.0	35.0	39.0	25.0	39.0
13	34.27740476041803	39.0	35.0	39.0	26.0	39.0
14	34.60333899103661	40.0	34.0	41.0	17.0	41.0
15	35.15537377739681	40.0	35.0	41.0	25.0	41.0
16	34.749357689840544	40.0	34.0	41.0	18.0	41.0
17	34.290156163963644	39.0	33.0	41.0	17.0	41.0
18	33.71298982181073	39.0	32.0	41.0	11.0	41.0
19	34.60539052344163	39.0	34.0	41.0	18.0	41.0
20	34.865930029915816	40.0	34.0	41.0	22.0	41.0
21	34.888675700069825	40.0	35.0	41.0	20.0	41.0
22	34.48100260557104	40.0	34.0	41.0	16.0	41.0
23	32.831785128376886	39.0	32.0	41.0	8.0	41.0
24	33.92707579997843	39.0	33.0	41.0	2.0	41.0
25	33.66409931823729	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8270451930334914
1101	2	1.1803641005039722
1101	3	0.9250502926041797
1101	4	0.8830373957172384
1101	5	1.0896923021485208
1101	6	0.7808700963269573
1101	7	0.8100485973265918
1101	8	0.7569277680227628
1101	9	0.9505505476135312
1101	10	1.2785786887167347
1101	11	0.8178388012731617
1101	12	0.8417923872013873
1101	13	0.8841884963321647
1101	14	0.07787614158831957
1101	15	0.5327560386842904
1101	16	1.1854457922957593
1101	17	1.7313220694071205
1101	18	-4.275840571082796
1101	19	-0.6382531923023436
1101	20	0.4410204348290847
1101	21	0.8413848550415395
1101	22	1.2554255030231332
1101	23	2.9453839127569523
1101	24	1.957823777686798
1101	25	2.2112916567538363
1102	1	0.9384219962825391
1102	2	1.180348660424162
1102	3	1.0071862760784782
1102	4	1.5005910099778674
1102	5	1.1968337073572641
1102	6	0.9301722684628828
1102	7	0.8553962533879087
1102	8	0.8415066837580341
1102	9	0.8823644637716299
1102	10	1.2944804127503815
1102	11	0.9868932267045878
1102	12	0.9399619728222248
1102	13	0.8503462494690908
1102	14	1.6484383687220898
1102	15	1.1323341420447122
1102	16	1.5100104351645882
1102	17	1.0830853336043091
1102	18	1.6434402498308316
1102	19	1.2900099304552128
1102	20	1.2165461801706101
1102	21	1.213236229683183
1102	22	1.6477198266406887
1102	23	3.247335354697505
1102	24	2.1487805937666877
1102	25	2.4445973537608268
1103	1	0.6905075085636945
1103	2	1.0527212619827608
1103	3	0.8508801389294014
1103	4	1.1893983257690195
1103	5	1.080923887371391
1103	6	0.7937671959146755
1103	7	0.7667627925928855
1103	8	0.6851263436489035
1103	9	0.7766591025654463
1103	10	1.106883197703148
1103	11	0.7581233848189584
1103	12	0.889040262023606
1103	13	0.7837849246725739
1103	14	1.513703991337124
1103	15	1.0649644846225712
1103	16	1.246492306365738
1103	17	1.6590238811392055
1103	18	1.610964887047487
1103	19	1.1171381940805603
1103	20	1.1177384496328742
1103	21	1.1769301038197213
1103	22	1.459506275600198
1103	23	2.861012737719925
1103	24	1.7325980567420913
1103	25	1.966255025317082
1104	1	0.6193862042703877
1104	2	1.0534328550968688
1104	3	0.7836990907763237
1104	4	1.4503366077585298
1104	5	1.3006863123697556
1104	6	0.7836548058162762
1104	7	0.6772313398998193
1104	8	0.8300702318269728
1104	9	0.8979356534500411
1104	10	1.2691563895353397
1104	11	0.8953429505501873
1104	12	1.0059570913533378
1104	13	0.736241072226747
1104	14	1.6767439837024085
1104	15	1.1053399727504782
1104	16	1.4131934661099308
1104	17	1.9834035328497492
1104	18	2.54151811532212
1104	19	1.664071990675346
1104	20	1.13844955448036
1104	21	1.217271468769063
1104	22	1.6305169431161488
1104	23	2.961605371330201
1104	24	1.8849863463330223
1104	25	1.8898948421283208
1105	1	0.5639317441088814
1105	2	1.01742962149887
1105	3	0.6981854788997239
1105	4	0.9372614822438692
1105	5	1.003139657572703
1105	6	0.6457643248567564
1105	7	0.5264948344984859
1105	8	0.5179009283160525
1105	9	0.7771813872607751
1105	10	1.1763349989136458
1105	11	0.7716060065839514
1105	12	0.7861989784479704
1105	13	0.7352363003786877
1105	14	1.4865791836640057
1105	15	0.8884325317658224
1105	16	1.1907690530996078
1105	17	1.6383762261837802
1105	18	1.534875820645773
1105	19	1.0528919674161301
1105	20	0.8652394338859182
1105	21	0.6763414711473459
1105	22	1.2620705974964537
1105	23	2.7712441989436485
1105	24	1.810753780379187
1105	25	1.7711681204337566
1106	1	-0.5533500661661286
1106	2	-0.08076718786980308
1106	3	-0.2868890983241634
1106	4	-2.560828978290651
1106	5	-0.4734473846658247
1106	6	-0.3778756323708876
1106	7	-0.3242542637398955
1106	8	-0.2879222316162995
1106	9	-0.24021566889706492
1106	10	-0.2848102591257131
1106	11	-0.2655366771317915
1106	12	-0.14508213004114623
1106	13	-0.18448737558248496
1106	14	0.4243655314338426
1106	15	-0.0966607542052742
1106	16	-0.9224400707822298
1106	17	-5.575206573742385
1106	18	-6.891392590840237
1106	19	-2.8364029705789235
1106	20	-1.4600233616499239
1106	21	-1.0001547713047074
1106	22	-1.4358305305162773
1106	23	-5.155545147214742
1106	24	-2.203891426938675
1106	25	-0.7170948430650554
1107	1	0.3071797363169573
1107	2	0.7024293184560015
1107	3	0.42470045400236245
1107	4	0.6892807047315657
1107	5	0.7846178368911438
1107	6	0.33926490595474945
1107	7	0.30473816409961074
1107	8	0.22681123932309788
1107	9	0.22266158261177793
1107	10	0.6043620516037578
1107	11	0.3426166598699467
1107	12	0.38738951160139123
1107	13	0.369618268370985
1107	14	1.1445591530619907
1107	15	0.521570163204025
1107	16	-5.807614452436503
1107	17	-1.084140443832311
1107	18	0.5506085304551078
1107	19	0.4955142558470129
1107	20	0.4231424197619944
1107	21	0.32667439298036527
1107	22	0.5645621415370883
1107	23	2.257020057804354
1107	24	1.3000765699472865
1107	25	1.0005165463652972
1108	1	0.3179523396950046
1108	2	0.5589158217581947
1108	3	0.36724136241332417
1108	4	1.0158128920160365
1108	5	0.6588874690232274
1108	6	0.29074589567287745
1108	7	0.210297286532942
1108	8	0.22332874577988804
1108	9	0.34874474043266446
1108	10	0.7801098273061271
1108	11	0.31734299944582745
1108	12	0.3818630867734498
1108	13	0.34355721783795445
1108	14	0.8100348502689272
1108	15	0.5514459784929997
1108	16	0.7964085444380302
1108	17	1.0626569779548873
1108	18	0.7472879363912455
1108	19	0.7261407922704279
1108	20	0.5773361193173372
1108	21	0.6563801012938981
1108	22	0.9843576954570281
1108	23	2.6648345207362567
1108	24	1.4087293888912455
1108	25	1.5384928955386599
1109	1	0.19585612897717652
1109	2	0.48109618021676326
1109	3	0.35400193777856614
1109	4	0.4196724773959417
1109	5	0.13431491050643984
1109	6	0.12464338805669684
1109	7	0.052726657471176
1109	8	0.038139153356155475
1109	9	0.14522255642734905
1109	10	0.6080268870787151
1109	11	0.29032711740394035
1109	12	0.21168164236329545
1109	13	0.10183195953613478
1109	14	-0.7475972350079871
1109	15	-0.1300317260700936
1109	16	-0.49473345386145695
1109	17	0.5901197344754934
1109	18	1.4324443694725204
1109	19	0.6353984619723079
1109	20	0.4636900706480631
1109	21	0.4410962782580796
1109	22	0.38811624952059987
1109	23	-4.333718759438575
1109	24	-1.452566094617218
1109	25	-0.17552783344010692
1110	1	0.3964248562797117
1110	2	0.7360349065892002
1110	3	0.5249787688277223
1110	4	1.0328179707982699
1110	5	0.7267392715618399
1110	6	0.40685848184704554
1110	7	0.2976888780667437
1110	8	0.2813293230915406
1110	9	0.30271700866934026
1110	10	0.694326906889323
1110	11	0.3767715129203353
1110	12	0.5227319408098197
1110	13	0.2181885315340253
1110	14	1.148460401770592
1110	15	0.6017092794153953
1110	16	1.07604361586084
1110	17	1.3155678509177164
1110	18	1.8067737909555177
1110	19	1.097943278871334
1110	20	0.5987655637718916
1110	21	0.6145510115479382
1110	22	0.8540484436477271
1110	23	2.2672457070596934
1110	24	1.1986294255583374
1110	25	1.447934151477554
1111	1	0.21453504794087053
1111	2	0.6002195299676956
1111	3	0.3311159900881968
1111	4	0.8031619371115895
1111	5	0.5176647549590072
1111	6	0.15436233384789944
1111	7	0.17338577511607411
1111	8	-0.2219922720355214
1111	9	-0.026196662782638214
1111	10	0.39469075698668377
1111	11	-0.021613862080627655
1111	12	0.1259916724173209
1111	13	0.1613111344834124
1111	14	-0.47437096676364376
1111	15	-0.07411841846426626
1111	16	0.2723532832897604
1111	17	0.8183111957663272
1111	18	1.427324697815557
1111	19	0.5774606662501469
1111	20	0.29223143851691447
1111	21	0.18860948993686577
1111	22	0.4689969078633496
1111	23	0.6167398666804331
1111	24	0.45964396155621756
1111	25	-0.03570468389345649
1112	1	0.4574331758535273
1112	2	0.8092359802952487
1112	3	0.5669886509610862
1112	4	1.145109814072626
1112	5	0.8483397775138783
1112	6	0.22956179226901696
1112	7	0.30944489835015077
1112	8	0.19132970420362483
1112	9	0.3475710684915754
1112	10	0.6952382282873231
1112	11	0.35890920150643524
1112	12	0.42504338560573274
1112	13	0.1791765594643948
1112	14	0.7205910140323013
1112	15	0.36117914220474745
1112	16	0.7894166844208428
1112	17	1.0639406568193905
1112	18	1.1356918034456953
1112	19	0.7076793536022663
1112	20	0.7033741359097121
1112	21	0.7010407941389474
1112	22	0.6065606933111667
1112	23	2.1914878347775613
1112	24	1.1056522397517767
1112	25	1.155114663671597
1113	1	0.3562275176591143
1113	2	0.7231198855699468
1113	3	0.41391991888692914
1113	4	0.51119922988741
1113	5	0.7799882166256893
1113	6	0.325057223075774
1113	7	0.09194553548864803
1113	8	0.10250324252699983
1113	9	0.14124969380964103
1113	10	0.49500506593633986
1113	11	0.2080805674909314
1113	12	0.22746951799300064
1113	13	0.13499757815783653
1113	14	0.7965771122770349
1113	15	0.4490549527865042
1113	16	0.6957957555335952
1113	17	1.256808801512534
1113	18	1.7545494849088072
1113	19	0.9608711140867356
1113	20	0.4692522846886362
1113	21	0.4148442480364096
1113	22	0.684294118608193
1113	23	0.5123480020921534
1113	24	0.40515006336756443
1113	25	0.9278357305043912
1114	1	-0.057476945390011025
1114	2	-2.815921095740645
1114	3	-0.7331123012777141
1114	4	0.47398089372706664
1114	5	0.6828560135787427
1114	6	0.43465519073890135
1114	7	0.30348130368398074
1114	8	0.3241199696243058
1114	9	0.49224893907503997
1114	10	0.5668128109138593
1114	11	0.3837362918027196
1114	12	0.374953022762611
1114	13	0.2424577795241376
1114	14	1.2309570238874983
1114	15	0.6283851452827278
1114	16	0.9834935815867212
1114	17	1.145857952630628
1114	18	1.4279555943686688
1114	19	0.9097436806512107
1114	20	0.7843802394356842
1114	21	0.61062979447982
1114	22	0.7599932207074431
1114	23	2.438865446576365
1114	24	1.2032128463930363
1114	25	1.250901042279807
1115	1	0.03886239037467831
1115	2	0.2842543330719849
1115	3	0.012809904288893392
1115	4	0.26838748377362975
1115	5	0.07021078842506512
1115	6	-0.2632558169984307
1115	7	-0.3002052253639391
1115	8	-0.35081282032361116
1115	9	-0.4025324744129293
1115	10	-0.12686018958709155
1115	11	-0.11695624095857227
1115	12	-0.2568721868721724
1115	13	-0.4196862307525606
1115	14	0.4699370752902112
1115	15	-0.1990207149722849
1115	16	0.2324219706704227
1115	17	0.7482880190810803
1115	18	1.2003873098211173
1115	19	0.4444897872277025
1115	20	-0.03650509194641671
1115	21	-0.061563632973225424
1115	22	0.23328696036512753
1115	23	1.9619345277409153
1115	24	0.6991696665787472
1115	25	0.35266477731259016
1116	1	0.38246761757495307
1116	2	0.19356489470133909
1116	3	0.35802763867669185
1116	4	1.0798132227495074
1116	5	0.5408874823984746
1116	6	0.28521048743287025
1116	7	0.18065080048484816
1116	8	0.09614737920621508
1116	9	0.17479583753758732
1116	10	0.6033004446894594
1116	11	0.21315636363140555
1116	12	0.30120935316345054
1116	13	0.23565749087546095
1116	14	0.9778662783383538
1116	15	0.37797882780438385
1116	16	0.6589794256664092
1116	17	0.8932791277345871
1116	18	1.4898003799340103
1116	19	0.6527636813367081
1116	20	0.3802013411732261
1116	21	0.3512152482909414
1116	22	0.7885640901643214
1116	23	2.139094276613612
1116	24	0.9914567119138837
1116	25	1.2237548893386219
1201	1	1.1204679233864319
1201	2	1.324820341652888
1201	3	1.0802254139234222
1201	4	1.7124113889908443
1201	5	1.491849022315705
1201	6	1.0493315595089996
1201	7	0.8809722053202051
1201	8	0.9698126710669399
1201	9	1.1365113692786508
1201	10	1.389311764760528
1201	11	0.9716343055908112
1201	12	1.075223754562714
1201	13	0.990523980951977
1201	14	1.9625996748188967
1201	15	1.504997700813398
1201	16	1.7357644740083416
1201	17	2.0631627023780723
1201	18	2.5866295791772558
1201	19	1.6948933756816658
1201	20	1.5030711951759983
1201	21	1.3219237065015008
1201	22	1.6631928648039676
1201	23	3.2671636826984596
1201	24	2.0847650420469606
1201	25	2.2844207458908343
1202	1	1.0808014734984361
1202	2	1.3600266426862007
1202	3	1.1874004988284028
1202	4	1.6681022626088833
1202	5	1.5523844656280872
1202	6	1.0117752331848493
1202	7	0.9124767938020284
1202	8	0.9186225561742418
1202	9	1.0255501726603953
1202	10	1.5226521795303825
1202	11	1.1078665478048393
1202	12	1.2203810636168413
1202	13	1.1345432019939636
1202	14	1.9662510947191763
1202	15	1.3973559636936628
1202	16	1.6826486419395508
1202	17	2.1398350052901307
1202	18	2.700771352426763
1202	19	1.8856463202585871
1202	20	1.5078070864976283
1202	21	1.4875220878874345
1202	22	1.9180336770013824
1202	23	3.5644915243055095
1202	24	2.2537367885604382
1202	25	2.4779493245453637
1203	1	0.6972681077098635
1203	2	1.0611088445938464
1203	3	0.7553941520261453
1203	4	1.3692410087548197
1203	5	1.1247866942211502
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2211	15	-0.394224252942351
2211	16	-0.5389250347414247
2211	17	-8.692661180020878E-4
2211	18	-5.61783604589737
2211	19	-1.9389418669476228
2211	20	-1.1208420040398792
2211	21	-0.7339457232713613
2211	22	-0.32052221917683
2211	23	-4.705872260693468
2211	24	-2.305439585851989
2211	25	-1.7099219200898332
2212	1	0.24602247119816312
2212	2	0.372061524411059
2212	3	0.20911135130205238
2212	4	0.6934358706014621
2212	5	0.5685035304133379
2212	6	0.13377736179403854
2212	7	0.12025459446807929
2212	8	-0.027971325790943524
2212	9	-0.25512219318572704
2212	10	-3.5920923530339017
2212	11	-1.1813161349293892
2212	12	-0.3909306403004891
2212	13	-0.13971010250015325
2212	14	0.5168132535408887
2212	15	9.084064443740658E-4
2212	16	0.3399858205023989
2212	17	0.8928296837671468
2212	18	1.3384530910198222
2212	19	0.5987324602661417
2212	20	0.1063163734135486
2212	21	-0.01362763573668957
2212	22	0.13667768427985294
2212	23	1.4504234774761073
2212	24	0.651848506210726
2212	25	0.5612206535528088
2213	1	-1.3022461455491836
2213	2	-3.570449547789991
2213	3	-1.600289977362074
2213	4	-3.3837548802482615
2213	5	-1.160472677229059
2213	6	-0.7796295287199158
2213	7	-0.7688565395312992
2213	8	-0.7024488723971842
2213	9	-0.6435926844824564
2213	10	-0.7553002014219956
2213	11	-0.7143715474332524
2213	12	-0.6323808411450642
2213	13	-1.0585758456250858
2213	14	-6.3363917624236805
2213	15	-2.725334512496815
2213	16	-1.882113262822351
2213	17	-0.7234745185193887
2213	18	-0.043371504716091636
2213	19	-0.5067346321223667
2213	20	-0.7315976754071372
2213	21	-0.9602874359220834
2213	22	-0.8325853706176645
2213	23	-5.502889428075445
2213	24	-2.92102511620147
2213	25	-2.037074484978451
2214	1	-0.06404404515221884
2214	2	0.07616419021312382
2214	3	-0.009892635519957338
2214	4	0.34767092750009
2214	5	0.019094681195142016
2214	6	-0.10459774639741681
2214	7	-0.29537179002122826
2214	8	-0.31783390330015493
2214	9	-0.20171840680797004
2214	10	0.1878968741018454
2214	11	-0.2035070180126226
2214	12	-0.25371343749701225
2214	13	-0.2913627658803506
2214	14	0.2781405475104961
2214	15	-0.1151273621023634
2214	16	0.3816192129326339
2214	17	0.41938651861442366
2214	18	1.1388060240513056
2214	19	0.14296739290150384
2214	20	-0.008148225630741024
2214	21	-0.07474500086857461
2214	22	0.32417433994964284
2214	23	1.7290152500814315
2214	24	0.7008868480214332
2214	25	0.5143765727573424
2215	1	-2.3439233357622875
2215	2	-4.325396272336164
2215	3	-2.455937175467838
2215	4	-4.126426545599486
2215	5	-4.683014113693147
2215	6	-2.3313442090671934
2215	7	-1.8382606437144133
2215	8	-2.060503331749988
2215	9	-1.7364162835353412
2215	10	-3.2667603434898744
2215	11	-2.2594840178357813
2215	12	-1.9326511647441222
2215	13	-1.9753260429415818
2215	14	-1.2410069952571448
2215	15	-1.8692650570903453
2215	16	-2.467507895813675
2215	17	-6.942985173610154
2215	18	-3.122491473145452
2215	19	-2.347952074900583
2215	20	-2.1974638948230805
2215	21	-2.101321374359337
2215	22	-1.7857917636721226
2215	23	-6.490817604871022
2215	24	-3.6897583442993387
2215	25	-3.1300460714030756
2216	1	-0.6629981269912868
2216	2	-0.12970336569062724
2216	3	-0.40363088205954156
2216	4	-2.815677362366955
2216	5	-0.8770085364983409
2216	6	-0.4916539826151407
2216	7	-0.7463144942964277
2216	8	-0.49033936147508683
2216	9	-0.45607673628992274
2216	10	-0.21180609742044965
2216	11	-0.7442004668223916
2216	12	-1.3839698610561442
2216	13	-0.7225140826248975
2216	14	-6.53962054649482
2216	15	-2.8933274566298124
2216	16	-1.2459891568876955
2216	17	-0.415547300270795
2216	18	0.32973790955028903
2216	19	-0.1868409534059552
2216	20	-0.6883775903501075
2216	21	-0.8179348358834275
2216	22	-0.2731856025913757
2216	23	0.8679021822087236
2216	24	0.1842155565222896
2216	25	0.3438374616675972
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77622.0
3	3.0
4	638.0
5	3851.0
6	4861.0
7	4110.0
8	4075.0
9	3803.0
10	3618.0
11	3512.0
12	3463.0
13	3520.0
14	3545.0
15	3666.0
16	3671.0
17	3967.0
18	4427.0
19	5420.0
20	6580.0
21	8216.0
22	10187.0
23	12354.0
24	14651.0
25	17308.0
26	19590.0
27	22605.0
28	25646.0
29	31127.0
30	36279.0
31	41266.0
32	53336.0
33	70296.0
34	91862.0
35	122635.0
36	175234.0
37	245647.0
38	619019.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.32276729116577	18.241211712753522	12.464845998623673	24.971174997457034
2	13.762586034265475	19.391792619297917	42.88432235468238	23.961298991754227
3	19.74115563995296	23.827116817274007	28.58474906884288	27.846978473930157
4	10.561208468409843	16.274369846437246	39.40071741738826	33.76370426776465
5	12.423800354230751	37.89928330453339	36.349481175173864	13.32743516606199
6	32.614321418311185	36.35819102416048	18.09214298934995	12.935344568178387
7	28.903805011877886	30.862293726749545	21.377357976353238	18.856543285019328
8	25.186407418595092	37.35569465295174	19.997490341790467	17.4604075866627
9	26.21013786262117	15.721131269586468	19.818340912872117	38.250389954920244
10	13.81593741695003	29.6852108021179	34.935672474769845	21.563179306162223
11	34.72329785083725	22.801042899223503	23.46835532157952	19.00730392835973
12	23.050705033030237	24.907154800668838	31.823490502564916	20.218649663736006
13	29.008806358337196	21.560901608490347	25.433178470267826	23.99711356290463
14	20.311315639309914	22.002855950512	28.56251437201736	29.123314038160725
15	23.826540982917777	30.148429009916843	22.957781025478553	23.067248981686827
16	21.854043302913148	28.470078428544742	28.210258678496338	21.46561959004577
17	21.739231652828224	28.39088167607999	28.243024330028497	21.62686234106329
18	21.17341130714965	27.60335552369732	30.236788690011494	20.986444479141532
19	23.097583827829986	27.341756338548024	27.91351750376196	21.647142329860028
20	23.035194653660422	27.526437864984487	28.33832518077112	21.100042300583976
21	22.73860330107363	26.94472393766708	27.703671064615556	22.613001696643735
22	22.36560861703964	27.91077220813912	27.60304512951908	22.12057404530215
23	20.95256644082502	27.322220682794317	29.420378896109057	22.304833980271603
24	22.34908026186074	27.768868374546262	28.102554550534485	21.77949681305851
25	22.32904466251844	27.135032372291	27.917452645374357	22.618470319816204
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	133.0
1	133.0
2	265.5
3	398.0
4	398.0
5	398.0
6	813.5
7	1229.0
8	1229.0
9	1229.0
10	2033.5
11	2838.0
12	2838.0
13	2838.0
14	5147.5
15	7457.0
16	7457.0
17	7457.0
18	13423.0
19	19389.0
20	19389.0
21	19389.0
22	30933.0
23	42477.0
24	42477.0
25	42477.0
26	60298.5
27	78120.0
28	78120.0
29	78120.0
30	101503.0
31	124886.0
32	124886.0
33	124886.0
34	144074.0
35	163262.0
36	163262.0
37	163262.0
38	180829.0
39	198396.0
40	198396.0
41	198396.0
42	212220.5
43	226045.0
44	226045.0
45	226045.0
46	239516.0
47	252987.0
48	252987.0
49	252987.0
50	239594.0
51	226201.0
52	226201.0
53	226201.0
54	196938.5
55	167676.0
56	167676.0
57	167676.0
58	141829.5
59	115983.0
60	115983.0
61	115983.0
62	93081.5
63	70180.0
64	70180.0
65	70180.0
66	53726.5
67	37273.0
68	37273.0
69	37273.0
70	26933.0
71	16593.0
72	16593.0
73	16593.0
74	11635.0
75	6677.0
76	6677.0
77	6677.0
78	4507.0
79	2337.0
80	2337.0
81	2337.0
82	1544.0
83	751.0
84	751.0
85	751.0
86	493.5
87	236.0
88	236.0
89	236.0
90	149.5
91	63.0
92	63.0
93	63.0
94	40.0
95	17.0
96	17.0
97	17.0
98	11.5
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10518786791628112
2	0.047059224232378336
3	0.03570597351286607
4	0.032413530804207516
5	0.03468418094810997
6	0.01725694109365864
7	0.020095253773536707
8	0.023501228989390388
9	0.028894023081158713
10	0.03530860973768314
11	0.020776448816707444
12	0.019584357491158656
13	0.015270122217743995
14	0.021287345099085496
15	0.020662916309512322
16	0.022082072649451355
17	0.025715112879695277
18	0.01901669495518304
19	0.03178910201463434
20	0.022990332707012333
21	0.027588399248414802
22	0.03598980478085388
23	0.028042529277195295
24	0.03826045492475633
25	0.03042671192829287
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1761610.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.4552825096427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.68764642181213	70.46702001560386
2	8.346120154001694	13.262866693912546
3	1.6541134278223624	3.9428414913185788
4	0.5160333604786925	1.6400630576493922
5	0.2359328993581902	0.937305758591205
6	0.14037694473924964	0.6692213881258549
7	0.0924654865352798	0.5142809948536555
8	0.055126260304261626	0.35040580689401674
9	0.040191895212843685	0.28741125498610054
>10	0.2067572925093857	2.85385159280944
>50	0.013152673134350496	0.7348598174199057
>100	0.009695992535736626	1.6736741664339045
>500	9.404087947001637E-4	0.5167781031530535
>1k	0.001374443623023315	1.791681351516736
>5k	7.233913805385868E-5	0.35773850673168756
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	6224	0.3533131623912217	No Hit
CCCATGTACTCTGCGTTGATACCAC	4778	0.27122915968914796	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2552	0.1448674791809765	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2190	0.12431809537865929	No Hit
GTCCTACAGTGGACATTTCTAAATT	1795	0.10189542520762257	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1770	0.10047626886768354	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.6766253597561324E-5	0.0
10	0.0	0.0	0.0	1.1353250719512265E-4	0.0
11	0.0	0.0	0.0	1.1353250719512265E-4	0.0
12	0.0	0.0	0.0	1.1353250719512265E-4	1.1353250719512265E-4
13	0.0	0.0	0.0	1.1353250719512265E-4	1.1353250719512265E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGAT	25	0.0060396846	18.996023	11
TAGGACC	650	0.0	13.888809	4
TCCAACG	160	0.0	13.654555	18
ATACCGA	60	4.09679E-4	12.665095	6
GTAGGAC	1220	0.0	12.384184	3
AGGACCT	860	0.0	12.263242	5
TGTAGGA	1340	0.0	11.984622	2
CTGTAGG	1305	0.0	11.95317	1
GGGCGAA	80	2.8730128E-5	11.872516	11
TAGGACG	795	0.0	11.714229	4
GGACCTG	850	0.0	11.6220875	6
CGGTTTC	90	7.4770633E-6	11.609012	13
GCGTTTA	90	7.4770633E-6	11.609012	13
GCCGGTT	90	7.479426E-6	11.608682	11
AGGACGT	825	0.0	11.516666	5
TGGCGAG	1040	0.0	11.416853	18
GGACGTG	855	0.0	11.22083	6
GTCCTAC	1225	0.0	11.180884	1
GTCTAGG	180	3.6379788E-12	11.096769	1
ACGACAG	60	0.005870431	11.084792	5
>>END_MODULE