FastQCFastQC Report
Thu 26 May 2016
SRR1513079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513079_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761610
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC71600.4064463757585391No Hit
CCCATGTACTCTGCGTTGATACCAC61920.3514966422760997No Hit
GTATCAACGCAGAGTACTTTTTTTT33690.19124550837018411No Hit
GAGTACATGGGAAGCAGTGGTATCA25910.1470813630712814No Hit
TATCAACGCAGAGTACTTTTTTTTT22300.12658874552256175No Hit
GGTATCAACGCAGAGTACTTTTTTT19830.1125674808839641No Hit
GTCCTACAGTGGACATTTCTAAATT19160.10876414189292748No Hit
GTCCTACAGTGTGCATTTCTCATTT18440.10467697163390308No Hit
CATGTACTCTGCGTTGATACCACTG18200.10331458154756161No Hit
GTATCAACGCAGAGTACATGGGAAG17990.10212249022201281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6500.013.6202084
TCCAACG1351.8189894E-1213.35747918
TGGCGAG10800.013.00596618
GGCGAGA7750.012.85454119
GTAGGAC11300.012.7207473
CCAACGA854.00086E-612.27842819
GTCCTAC11500.011.8477541
TATGGCG11950.011.83612116
ATGGCGA12250.011.77839717
TGTAGGA13350.011.5593262
TAGGACA4900.011.4622554
AATCCCG2000.011.38545119
GGCGAGG3950.011.04917319
CTAGTAC1303.1690433E-810.9840383
GGACCTG9400.010.9372976
CTGTAGG13500.010.7983031
GGACGTG6700.010.7982396
AGGACCT10150.010.785645
TCCTACA12500.010.7450362
TGGACCG909.319985E-510.5772235
Produced by FastQC (version 0.11.5)