##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513079_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1761610 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.640929604168914 31.0 31.0 34.0 27.0 34.0 2 30.799068465778465 33.0 31.0 34.0 27.0 34.0 3 30.796773406145515 33.0 31.0 34.0 27.0 34.0 4 34.02731705655622 37.0 35.0 37.0 30.0 37.0 5 33.965413457008076 37.0 35.0 37.0 30.0 37.0 6 33.925604986347714 37.0 35.0 37.0 30.0 37.0 7 33.843305839544506 37.0 35.0 37.0 29.0 37.0 8 33.845378943125894 37.0 35.0 37.0 29.0 37.0 9 35.41656552812484 39.0 37.0 39.0 30.0 39.0 10 35.32707352932829 39.0 35.0 39.0 29.0 39.0 11 35.329776738324604 39.0 35.0 39.0 29.0 39.0 12 35.249749376990366 39.0 35.0 39.0 29.0 39.0 13 35.223225344996905 39.0 35.0 39.0 29.0 39.0 14 36.40667060246025 40.0 37.0 41.0 28.0 41.0 15 36.40618581865453 40.0 37.0 41.0 29.0 41.0 16 36.37133077128309 40.0 37.0 41.0 28.0 41.0 17 36.2836433716884 40.0 37.0 41.0 28.0 41.0 18 36.21126185705122 40.0 37.0 41.0 27.0 41.0 19 36.28800301996469 40.0 37.0 41.0 27.0 41.0 20 36.18461407462492 40.0 37.0 41.0 27.0 41.0 21 36.204894386385185 40.0 37.0 41.0 27.0 41.0 22 36.17851056703811 40.0 37.0 41.0 27.0 41.0 23 36.11704577063028 40.0 37.0 41.0 27.0 41.0 24 35.98765901646789 40.0 37.0 41.0 27.0 41.0 25 35.971106544581374 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5791391515334254 1101 2 0.5183520831861905 1101 3 0.5680728504302479 1101 4 0.8113287101198594 1101 5 0.6405324849985661 1101 6 0.5602412831850998 1101 7 0.48741281567900074 1101 8 0.6285801144030074 1101 9 0.5187509655606348 1101 10 0.46410485483745845 1101 11 0.5133562625140513 1101 12 0.4754659958267382 1101 13 0.4925609495437726 1101 14 0.5553460592489543 1101 15 0.6157654114506599 1101 16 0.5986522026639918 1101 17 0.5308389358461127 1101 18 0.42889909214193267 1101 19 0.445873801724737 1101 20 0.6000747474341637 1101 21 0.5837941621403857 1101 22 0.6072920286728305 1101 23 0.5859826231966139 1101 24 0.6641213667458814 1101 25 0.733220782501121 1102 1 0.6068920575618435 1102 2 0.5804447182822869 1102 3 0.6045175298283212 1102 4 0.9037363129284799 1102 5 0.674648828691339 1102 6 0.534169787683048 1102 7 0.5494223009874872 1102 8 0.5193418330340407 1102 9 0.5338832353416123 1102 10 0.40713393502374373 1102 11 0.4828945432172489 1102 12 0.47799363604850953 1102 13 0.4951480836510598 1102 14 0.5064501595250519 1102 15 0.6345517390191802 1102 16 0.6371099001741314 1102 17 0.6183579827427295 1102 18 0.5756542177172292 1102 19 0.6046112791471643 1102 20 0.7543089626793602 1102 21 0.8026043155935341 1102 22 0.7192453560029506 1102 23 0.6466156749680536 1102 24 0.6370948527291915 1102 25 0.7224154180224573 1103 1 -0.013263413157190485 1103 2 0.2265307378521193 1103 3 0.4545149764565046 1103 4 -0.47736867918145975 1103 5 -0.12674469161276392 1103 6 0.0324243252786971 1103 7 0.22791822673712403 1103 8 0.21356314350345684 1103 9 0.28294801001148784 1103 10 0.31776098389302376 1103 11 0.4041134298173503 1103 12 0.37237418663447386 1103 13 0.40287966530549824 1103 14 0.5080103966698104 1103 15 0.3870872654496793 1103 16 0.23835024638313485 1103 17 0.41613968101995624 1103 18 0.4746782371871845 1103 19 0.4547057821185234 1103 20 0.5242936114129861 1103 21 0.46545780593994834 1103 22 0.4892982827454446 1103 23 0.43513399421176047 1103 24 0.4503784804244404 1103 25 0.5177917271897954 1104 1 0.40210444580477755 1104 2 0.32780638988225164 1104 3 0.43161804621093935 1104 4 0.32219287958752574 1104 5 0.23288670037988624 1104 6 0.29483045879520375 1104 7 0.23669814832594227 1104 8 0.2978565495737229 1104 9 0.17112136019741797 1104 10 0.1170589896567833 1104 11 0.20922929881541563 1104 12 0.11574707696202324 1104 13 0.28172878759075815 1104 14 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1107 12 0.14607401450216884 1107 13 0.15312261358631218 1107 14 0.041960965550039475 1107 15 0.14927185855389524 1107 16 0.07908730577067047 1107 17 -0.05103972323895789 1107 18 -0.0345110753597595 1107 19 -0.002024818116495908 1107 20 0.10180344155739363 1107 21 0.08853537796053246 1107 22 0.1045341818758061 1107 23 0.08965586063860087 1107 24 0.12721373363955024 1107 25 0.12192771549582915 1108 1 -0.5616910509069903 1108 2 -0.34439793412197517 1108 3 -0.12055414249362428 1108 4 -1.2093111230636993 1108 5 -0.7422244692852615 1108 6 -0.5300555192941161 1108 7 -0.48343653199165715 1108 8 -0.44947374860817746 1108 9 -0.2837307169164376 1108 10 -0.18564765553201568 1108 11 -0.07386517027303086 1108 12 0.07840996055509208 1108 13 -0.023155207508807507 1108 14 -0.1161518133889956 1108 15 -0.10981893620972016 1108 16 -0.13418567157796701 1108 17 -0.08219723170790161 1108 18 -0.3359150867072884 1108 19 -0.2508741139265709 1108 20 -0.15176331119972986 1108 21 -0.18368211539073798 1108 22 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2211 8 0.16988610995184672 2211 9 0.14646716305311713 2211 10 0.2303863496935179 2211 11 0.24475021917075424 2211 12 0.16761378529296422 2211 13 0.1911408749071768 2211 14 0.2694937811270677 2211 15 0.19813601641259737 2211 16 0.23002057239030904 2211 17 0.08395917349880477 2211 18 0.26551487461117773 2211 19 0.022090267155832066 2211 20 0.21099610222189824 2211 21 0.11368380039912296 2211 22 0.11251522234014999 2211 23 0.16305802096073307 2211 24 -3.379091176965126 2211 25 -2.303570487688816 2212 1 -0.09889667798211477 2212 2 -0.19614746522101356 2212 3 -0.585120914912892 2212 4 0.22631585979752344 2212 5 -0.0392933604910084 2212 6 -0.11078965409770092 2212 7 -0.16045821017600304 2212 8 -0.2974031373300363 2212 9 -0.241142647233076 2212 10 -0.43050912050646417 2212 11 -0.3943286276317082 2212 12 -0.4962697830552685 2212 13 -0.41495079173370186 2212 14 -0.41967670962447556 2212 15 -0.5175769240018226 2212 16 -0.6071577325937412 2212 17 -0.42451312135438 2212 18 -0.5336154619598616 2212 19 -0.5632115802665325 2212 20 -0.9305597613921037 2212 21 -0.7906178954007288 2212 22 -0.5925715067702413 2212 23 -0.5753554918028883 2212 24 -0.4350189984590145 2212 25 -0.5292270802254819 2213 1 -0.2443151041846079 2213 2 -0.6653224574962238 2213 3 -0.4110947536192455 2213 4 0.01832533554167526 2213 5 -0.28762274559760925 2213 6 -0.39526771569003927 2213 7 -0.42882827918413824 2213 8 -0.49801906070010915 2213 9 -0.5947192256654574 2213 10 -0.6082939078172132 2213 11 -0.6253923314117102 2213 12 -0.5119243303824916 2213 13 -0.5173462495563399 2213 14 -0.5968530408635289 2213 15 -0.6373907565283403 2213 16 -0.6462240628028297 2213 17 -0.6605212066360764 2213 18 -0.7366417777493339 2213 19 -0.7133884779902999 2213 20 -5.94603792598166 2213 21 -3.191443933097709 2213 22 -1.7090165577538627 2213 23 -1.1584446358959966 2213 24 -0.8242766460025308 2213 25 -0.7781178001760409 2214 1 -0.03720453185156103 2214 2 -0.37329793611538875 2214 3 -0.09064251503826881 2214 4 0.21544206334429816 2214 5 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2215 16 -0.6329367699851502 2215 17 -0.7128732192643383 2215 18 -0.6436583523349668 2215 19 -0.6421059207685147 2215 20 -0.5215252977259368 2215 21 -0.5427185976596647 2215 22 -0.6382529429156634 2215 23 -0.712351502510046 2215 24 -0.8031183981730123 2215 25 -0.7549903337198458 2216 1 -0.8709539017875478 2216 2 -0.5019085777359287 2216 3 -2.1108608467953545 2216 4 -0.6406037191654548 2216 5 -0.7447250073412803 2216 6 -0.7230257864420224 2216 7 -0.5819918206494421 2216 8 -0.7172516143440291 2216 9 -0.7655340333205913 2216 10 -0.8258345032163987 2216 11 -0.8445480437643127 2216 12 -0.6785529791356311 2216 13 -0.7664227042248157 2216 14 -0.7866104350255227 2216 15 -0.8319717540152922 2216 16 -0.7998844146296236 2216 17 -0.8997358789732175 2216 18 -0.9021255395896617 2216 19 -0.886103053671718 2216 20 -0.8356247228113531 2216 21 -0.9476511629449931 2216 22 -0.9549947135628756 2216 23 -0.769383646141506 2216 24 -0.809663752130497 2216 25 -0.7847271177867157 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42581.0 3 2.0 4 756.0 5 5598.0 6 6761.0 7 5072.0 8 4100.0 9 3723.0 10 3239.0 11 2968.0 12 2721.0 13 2491.0 14 2519.0 15 2515.0 16 2557.0 17 2764.0 18 3020.0 19 3532.0 20 4690.0 21 6045.0 22 7704.0 23 9197.0 24 10688.0 25 12024.0 26 12917.0 27 14313.0 28 16019.0 29 19640.0 30 24691.0 31 31404.0 32 39551.0 33 51873.0 34 69595.0 35 95140.0 36 140164.0 37 235434.0 38 863602.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.09063062673672 19.063256037207694 13.688090967306776 27.158022368748814 2 13.335961459700767 19.879825702962265 40.72418135662444 26.060031480712524 3 17.47867008021072 23.920334239701184 29.09849512661713 29.502500553470973 4 10.190053417044636 16.91407292192937 37.702442651892305 35.19343100913369 5 12.623964326208077 37.949108978460714 35.850139641729115 13.576787053602098 6 32.76210085177828 36.899187531939546 17.529848701253197 12.808862915028978 7 28.538617080821854 30.84018987521415 21.42630073717996 19.19489230678403 8 25.101031351659515 37.97115077222193 19.479560067163064 17.448257808955493 9 25.647657801819157 15.414772423990849 19.751760041520967 39.18580973266903 10 13.85309695328599 30.165874759447536 34.380126818690144 21.60090146857633 11 34.67256751709002 22.700865258743132 23.32758462130596 19.298982602860885 12 22.760853187816114 25.240073326327707 31.634099580968083 20.3649739048881 13 28.839873037932502 21.40120388016607 25.518103902931127 24.240819178970305 14 20.434166068719968 22.08342037620687 27.712672231355416 29.769741323717746 15 23.61380987494955 30.300367448168124 22.952652943984734 23.133169732897596 16 21.85989316531671 28.7067257934397 27.676359831567044 21.757021209676548 17 21.892482862429873 28.683690393251375 27.55314733398857 21.87067941033018 18 21.568354575621903 28.09991809061382 29.082489909013553 21.24923742475072 19 22.963506393436386 27.410946435273804 27.80354130337635 21.82200586791346 20 23.02484658919965 27.566373942018952 28.12523770868694 21.283541760094458 21 22.589052060331174 26.98860701290297 27.639318577891814 22.78302234887404 22 22.32122887585788 27.937511708039803 27.33794653754236 22.40331287855995 23 21.787088237247552 27.734966391406008 27.529017604988184 22.948927766358253 24 22.22190685307254 27.80948499208676 28.139979246186996 21.828628908653705 25 22.332922724098978 27.176560078564492 27.57886251781041 22.911654679526116 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 78.0 1 78.0 2 177.0 3 276.0 4 276.0 5 276.0 6 831.0 7 1386.0 8 1386.0 9 1386.0 10 2069.5 11 2753.0 12 2753.0 13 2753.0 14 4983.0 15 7213.0 16 7213.0 17 7213.0 18 12860.0 19 18507.0 20 18507.0 21 18507.0 22 29904.0 23 41301.0 24 41301.0 25 41301.0 26 59065.5 27 76830.0 28 76830.0 29 76830.0 30 99956.5 31 123083.0 32 123083.0 33 123083.0 34 142084.0 35 161085.0 36 161085.0 37 161085.0 38 177780.5 39 194476.0 40 194476.0 41 194476.0 42 207650.5 43 220825.0 44 220825.0 45 220825.0 46 237678.0 47 254531.0 48 254531.0 49 254531.0 50 242656.0 51 230781.0 52 230781.0 53 230781.0 54 201055.0 55 171329.0 56 171329.0 57 171329.0 58 144999.0 59 118669.0 60 118669.0 61 118669.0 62 95609.0 63 72549.0 64 72549.0 65 72549.0 66 55412.5 67 38276.0 68 38276.0 69 38276.0 70 27816.0 71 17356.0 72 17356.0 73 17356.0 74 12027.0 75 6698.0 76 6698.0 77 6698.0 78 4586.0 79 2474.0 80 2474.0 81 2474.0 82 1649.0 83 824.0 84 824.0 85 824.0 86 538.0 87 252.0 88 252.0 89 252.0 90 153.5 91 55.0 92 55.0 93 55.0 94 29.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18528505174244014 2 0.06624621794835406 3 0.0 4 0.0 5 0.01799490239042694 6 0.3599548140621363 7 0.0018165201151219622 8 0.029688750631524572 9 0.032470297057805075 10 0.002270650143902453 11 0.003178910201463434 12 0.009593496857987863 13 0.007890509250061024 14 0.0014759225935365944 15 5.676625359756132E-5 16 0.011182951958719581 17 0.023898592764573318 18 0.06403233405804917 19 0.02878049057396359 20 0.0 21 0.0 22 0.0 23 3.9736377518292926E-4 24 3.4059752158536793E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1761610.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.74775455260696 #Duplication Level Percentage of deduplicated Percentage of total 1 86.33674385125491 65.39814482116155 2 9.893619241426357 14.988388838730263 3 2.069042997924671 4.701760834967642 4 0.7123201253539981 2.158266000727875 5 0.31892708008370346 1.2079005091179997 6 0.16290845560421396 0.7403969825791361 7 0.10013381395520687 0.5309438093326794 8 0.07324463255384442 0.44384931591875937 9 0.04600338662730613 0.3136187914950479 >10 0.2568112448066717 3.394188448145912 >50 0.0161132065105567 0.8395054934257363 >100 0.01105054745932429 1.771063934234154 >500 0.001277660474824687 0.6389182076007642 >1k 0.0016534429674201826 2.112933197460782 >5k 1.5031299703819842E-4 0.7601208151016128 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 7160 0.4064463757585391 No Hit CCCATGTACTCTGCGTTGATACCAC 6192 0.3514966422760997 No Hit GTATCAACGCAGAGTACTTTTTTTT 3369 0.19124550837018411 No Hit GAGTACATGGGAAGCAGTGGTATCA 2591 0.1470813630712814 No Hit TATCAACGCAGAGTACTTTTTTTTT 2230 0.12658874552256175 No Hit GGTATCAACGCAGAGTACTTTTTTT 1983 0.1125674808839641 No Hit GTCCTACAGTGGACATTTCTAAATT 1916 0.10876414189292748 No Hit GTCCTACAGTGTGCATTTCTCATTT 1844 0.10467697163390308 No Hit CATGTACTCTGCGTTGATACCACTG 1820 0.10331458154756161 No Hit GTATCAACGCAGAGTACATGGGAAG 1799 0.10212249022201281 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 5.6766253597561324E-5 0.0 9 0.0 0.0 0.0 5.6766253597561324E-5 0.0 10 0.0 0.0 0.0 5.6766253597561324E-5 0.0 11 0.0 0.0 0.0 5.6766253597561324E-5 0.0 12 0.0 0.0 0.0 5.6766253597561324E-5 0.0 13 0.0 0.0 0.0 5.6766253597561324E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 650 0.0 13.620208 4 TCCAACG 135 1.8189894E-12 13.357479 18 TGGCGAG 1080 0.0 13.005966 18 GGCGAGA 775 0.0 12.854541 19 GTAGGAC 1130 0.0 12.720747 3 CCAACGA 85 4.00086E-6 12.278428 19 GTCCTAC 1150 0.0 11.847754 1 TATGGCG 1195 0.0 11.836121 16 ATGGCGA 1225 0.0 11.778397 17 TGTAGGA 1335 0.0 11.559326 2 TAGGACA 490 0.0 11.462255 4 AATCCCG 200 0.0 11.385451 19 GGCGAGG 395 0.0 11.049173 19 CTAGTAC 130 3.1690433E-8 10.984038 3 GGACCTG 940 0.0 10.937297 6 CTGTAGG 1350 0.0 10.798303 1 GGACGTG 670 0.0 10.798239 6 AGGACCT 1015 0.0 10.78564 5 TCCTACA 1250 0.0 10.745036 2 TGGACCG 90 9.319985E-5 10.577223 5 >>END_MODULE