FastQCFastQC Report
Thu 26 May 2016
SRR1513078_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513078_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530043
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC71650.4682874925737381No Hit
CCCATGTACTCTGCGTTGATACCAC59320.3877015221140844No Hit
GAGTACATGGGAAGCAGTGGTATCA32550.21273911909665283No Hit
GCGTTGATACCACTGCTTCCCATGT19970.13051920763011235No Hit
CATGTACTCTGCGTTGATACCACTG19580.12797025965936903No Hit
GTATCAACGCAGAGTACATGGGAAG19520.12757811381771622No Hit
GTATCAACGCAGAGTACTTTTTTTT18210.11901626294162976No Hit
GTCCTACAGTGGACATTTCTAAATT15690.10254613759221147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAC1006.5847416E-1014.2525743
GTGCGAA400.005285324514.2474511
TAGGACC4100.013.4418934
CGTGCGC655.4598662E-513.15192210
TGTAGGA9400.012.0295562
AGGACGT5850.012.0184795
GTAGGAC8700.011.9044493
GGACGTG5950.011.8133986
CTGTAGG9450.011.4736431
GTCCTAC9750.011.4132561
TATAGGA2600.011.329712
TCCTACA11900.011.2590732
TAGGACG6400.011.1351884
GACGTGA3000.011.0817137
TAGACAG2500.011.0212715
GGACCTG6400.010.9827686
ATAGGAC2000.010.9269733
TGGCGAG6650.010.85626418
CCAACGA803.7734205E-410.68663519
TTAGACC1355.6668796E-810.5578084
Produced by FastQC (version 0.11.5)