##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513078_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1530043
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.775022009185363	31.0	29.0	34.0	22.0	34.0
2	28.510143178982553	31.0	28.0	34.0	16.0	34.0
3	28.84862647651079	31.0	29.0	34.0	20.0	34.0
4	31.673814396065993	35.0	32.0	37.0	17.0	37.0
5	31.900481881881753	35.0	32.0	37.0	19.0	37.0
6	32.42778340216582	35.0	33.0	37.0	25.0	37.0
7	32.44079022615704	35.0	33.0	37.0	24.0	37.0
8	32.42292471518774	35.0	33.0	37.0	23.0	37.0
9	33.74140922836809	38.0	34.0	39.0	22.0	39.0
10	33.2088346536666	38.0	33.0	39.0	17.0	39.0
11	33.60253796788718	38.0	34.0	39.0	22.0	39.0
12	33.453164388190395	38.0	33.0	39.0	18.0	39.0
13	33.51944357119375	38.0	34.0	39.0	19.0	39.0
14	33.79601292251264	39.0	33.0	41.0	11.0	41.0
15	34.310635714159666	39.0	33.0	41.0	17.0	41.0
16	33.8658534433346	39.0	33.0	41.0	11.0	41.0
17	33.37829394337283	38.0	32.0	41.0	10.0	41.0
18	32.744968605457494	38.0	31.0	41.0	10.0	41.0
19	33.665926382461144	39.0	32.0	41.0	12.0	41.0
20	33.94274605354229	39.0	33.0	41.0	11.0	41.0
21	33.97499678113622	39.0	34.0	41.0	10.0	41.0
22	33.53483202759661	39.0	33.0	41.0	2.0	41.0
23	31.787839296019786	38.0	30.0	41.0	2.0	41.0
24	32.86887231273892	38.0	32.0	41.0	2.0	41.0
25	32.59027033880747	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6717437345857995
1101	2	1.071574208133466
1101	3	0.7906914538133663
1101	4	0.8327586296712965
1101	5	1.1199790075040248
1101	6	0.7947062434301344
1101	7	0.8048335799421054
1101	8	0.8298437905397265
1101	9	0.9453927217584521
1101	10	1.3608911755162296
1101	11	1.0121382056634474
1101	12	0.9686074217013996
1101	13	0.9652435014113578
1101	14	-0.14360968773400629
1101	15	0.4341677261014567
1101	16	1.1593517919291827
1101	17	1.8823724987053083
1101	18	-3.9413896798743444
1101	19	-0.5296626075909359
1101	20	0.28521833751216263
1101	21	0.7086321467583403
1101	22	1.2741039110673569
1101	23	3.1290177575204154
1101	24	2.2217814769482516
1101	25	2.449048601932418
1102	1	0.9305264240944062
1102	2	1.162432762585798
1102	3	1.0757554148900432
1102	4	1.593094561992821
1102	5	1.386630583638894
1102	6	1.068909292514789
1102	7	1.0573153175513497
1102	8	0.8757688344446279
1102	9	1.1668620498254754
1102	10	1.3797484179464732
1102	11	1.1068778171135705
1102	12	1.1445265205062256
1102	13	1.1042807710387308
1102	14	2.00453793525098
1102	15	1.395469053840472
1102	16	1.7913692308894085
1102	17	1.5204910112002636
1102	18	1.921572004980682
1102	19	1.5566718623726388
1102	20	1.4337527336745026
1102	21	1.5082310233394267
1102	22	1.6794659471616313
1102	23	3.404279649428929
1102	24	2.379483285762035
1102	25	2.587442844068832
1103	1	0.766411551669492
1103	2	1.0511893936382108
1103	3	0.8932583218030103
1103	4	1.3792642379542954
1103	5	1.2019539859337094
1103	6	0.7132567179771527
1103	7	0.7416627430137126
1103	8	0.725723859564745
1103	9	0.8095859918188495
1103	10	1.1352121418665249
1103	11	0.9016809148955431
1103	12	0.9166427970249487
1103	13	0.8715929405830565
1103	14	1.7361582156309652
1103	15	1.2231435673439108
1103	16	1.5226857004710297
1103	17	1.882701230370536
1103	18	1.7661012605915758
1103	19	1.3308412750665255
1103	20	1.1910638940263993
1103	21	1.2195173926599736
1103	22	1.4764775089966093
1103	23	3.1552869551045433
1103	24	1.981547654687688
1103	25	2.131898472510507
1104	1	0.7453494174665245
1104	2	1.1154096817404948
1104	3	0.8743869743741186
1104	4	1.508020474051314
1104	5	1.2447735008679466
1104	6	0.781459642193731
1104	7	0.7062628769143302
1104	8	0.7887348955667335
1104	9	0.9470932807634114
1104	10	1.3202045613641857
1104	11	0.8828493057125542
1104	12	1.0815820882155833
1104	13	0.8608133366697004
1104	14	1.6618910623147656
1104	15	1.3509535148635052
1104	16	1.5656252940178845
1104	17	1.9602825533949613
1104	18	2.766245009376753
1104	19	1.790176350547597
1104	20	1.1800710857850376
1104	21	1.3648239170752419
1104	22	1.7178961643753112
1104	23	3.305825105980297
1104	24	2.2375343510140553
1104	25	2.159848799703127
1105	1	0.49170253431113053
1105	2	0.9835012876473286
1105	3	0.6546803804062087
1105	4	0.6346226420980443
1105	5	0.8329572606923854
1105	6	0.4658947180199604
1105	7	0.3656468186970301
1105	8	0.43082863043865416
1105	9	0.6279546055043426
1105	10	1.1674402191765196
1105	11	0.6289829846286423
1105	12	0.5483734534749445
1105	13	0.5542945950853166
1105	14	1.1468740821577939
1105	15	0.6639450912838925
1105	16	1.170510532987457
1105	17	1.5548044815851938
1105	18	1.3681387169816333
1105	19	1.1271757713315864
1105	20	0.7785941266840908
1105	21	0.5563144646404581
1105	22	1.2322287985666094
1105	23	2.6189580237888563
1105	24	1.7339943917010174
1105	25	1.6554764781182598
1106	1	-0.6034865807583536
1106	2	-0.18387682028368957
1106	3	-0.34171621130395025
1106	4	-2.9842332153563973
1106	5	-0.4927217356282618
1106	6	-0.521038697982096
1106	7	-0.35776000741073233
1106	8	-0.25721332209909065
1106	9	-0.30586392329151124
1106	10	-0.07174541331173856
1106	11	-0.21797952653060548
1106	12	-0.08786993642156915
1106	13	-0.3127262444763659
1106	14	0.38234497284658175
1106	15	-0.18264251075456173
1106	16	-1.1918881535187822
1106	17	-6.038565323109939
1106	18	-7.231219622260717
1106	19	-3.239539030536193
1106	20	-1.8399208258377726
1106	21	-1.4645844749121792
1106	22	-1.491766973176567
1106	23	-5.323178240141637
1106	24	-2.5380177103377832
1106	25	-1.0568247872518022
1107	1	0.45478113087422756
1107	2	0.702981612571854
1107	3	0.4450620364686486
1107	4	0.7408564826246575
1107	5	0.8499792079128632
1107	6	0.3744328857805286
1107	7	0.32942407789125383
1107	8	0.39104363822900723
1107	9	0.4877698377417232
1107	10	0.9348039575916616
1107	11	0.4457267262454323
1107	12	0.5871255319796944
1107	13	0.5292136672162684
1107	14	1.1997651302279664
1107	15	0.7172568946720048
1107	16	-5.809031710415599
1107	17	-1.1172383741419694
1107	18	0.552622772194546
1107	19	0.5114586130324668
1107	20	0.5458015940221159
1107	21	0.6320292101008889
1107	22	0.6931063426945485
1107	23	2.6100944873826677
1107	24	1.5621160928981226
1107	25	1.2363236430162345
1108	1	0.7250628674589663
1108	2	0.9226729462697918
1108	3	0.8039491112766974
1108	4	1.3645438432174544
1108	5	1.1312171438284473
1108	6	0.6622698758718926
1108	7	0.7135870851189807
1108	8	0.7986679385121107
1108	9	0.9374642812925345
1108	10	1.3345443787086353
1108	11	0.8690986780534402
1108	12	0.8643796391302132
1108	13	0.7395074142672655
1108	14	1.3705134787888582
1108	15	1.0439067252386423
1108	16	1.5652876741552433
1108	17	1.5905597830021136
1108	18	1.1226242869073673
1108	19	1.1632623276981064
1108	20	1.0623632361316666
1108	21	1.1307969979231345
1108	22	1.5305696142597682
1108	23	3.2288034024729626
1108	24	2.0190838388982115
1108	25	2.3366254461947165
1109	1	0.001180949390597874
1109	2	0.38791863943311
1109	3	0.18773471898152394
1109	4	0.277321752875622
1109	5	0.053018554571799825
1109	6	0.004846517217522717
1109	7	-0.018293849498491
1109	8	-0.26001678069570033
1109	9	-0.08482529412798101
1109	10	0.22614214729246385
1109	11	-0.01111344213104104
1109	12	0.027822395505687325
1109	13	-0.06175920266165491
1109	14	-1.3980316921281286
1109	15	-0.5575704858302615
1109	16	-0.7014966109776921
1109	17	0.447046319898476
1109	18	1.2043953463213626
1109	19	0.22301273464330507
1109	20	0.14079802316367562
1109	21	0.1736617225568864
1109	22	0.07930333645184362
1109	23	-4.668756452383249
1109	24	-1.9027757310963622
1109	25	-0.70884813627951
1110	1	0.6101580918457046
1110	2	0.9117749965455211
1110	3	0.7724571216931437
1110	4	1.3801600946172314
1110	5	1.1054823934639764
1110	6	0.7235675210788486
1110	7	0.56986194434905
1110	8	0.4673790815216847
1110	9	0.6850745928879576
1110	10	1.141945265321489
1110	11	0.7661766785922239
1110	12	0.8062519584270547
1110	13	0.6382412111993787
1110	14	1.417574345276634
1110	15	0.861498740704846
1110	16	1.4662546750954704
1110	17	1.645625948830677
1110	18	2.3393820820752325
1110	19	1.4468094129946465
1110	20	1.1636388480714928
1110	21	0.9348991670477744
1110	22	1.236019100832479
1110	23	2.575444237109231
1110	24	1.7044688235270868
1110	25	2.075005489350076
1111	1	0.7060118344276667
1111	2	1.055964408097438
1111	3	0.7564462060435275
1111	4	1.3821517987365937
1111	5	1.0240475103430207
1111	6	0.7149061406792114
1111	7	0.6797061421097439
1111	8	0.37496662459698626
1111	9	0.5741469669061416
1111	10	0.9418986016056223
1111	11	0.6979759259273948
1111	12	0.6928744873619053
1111	13	0.5918121210127651
1111	14	-0.02532052395304163
1111	15	0.4681497580343077
1111	16	1.0468155121797906
1111	17	1.5421560211937475
1111	18	2.1407433358652312
1111	19	1.3240060673892131
1111	20	1.0400881641033024
1111	21	0.9031283002708861
1111	22	1.0904287974742104
1111	23	0.8545703773353353
1111	24	0.9323192846172077
1111	25	0.5649431354499086
1112	1	0.5371717855876206
1112	2	0.9166992288322575
1112	3	0.711525201122523
1112	4	1.2574779540627503
1112	5	0.9153532416487558
1112	6	0.37718016295323054
1112	7	0.39912154152514745
1112	8	0.35689876652912744
1112	9	0.4581133708725389
1112	10	0.6964285161355335
1112	11	0.4196394475909173
1112	12	0.4427927172801347
1112	13	0.3480543972550407
1112	14	0.7160284973959818
1112	15	0.39265340253795245
1112	16	0.7589345042987858
1112	17	1.0580845371222622
1112	18	1.0294250245469172
1112	19	0.6860055493886961
1112	20	0.5269065309216714
1112	21	0.6165274936700769
1112	22	0.4965604436058584
1112	23	2.1703938131587677
1112	24	1.168227080525007
1112	25	1.1966980472048192
1113	1	0.296262450102013
1113	2	0.5816054337384635
1113	3	0.4352021644605024
1113	4	0.4233830094593358
1113	5	0.8082062267051278
1113	6	0.26076956789857064
1113	7	0.20841691658323214
1113	8	0.12631007085568058
1113	9	0.20481134333322615
1113	10	0.533452998244762
1113	11	0.21255895745957787
1113	12	0.2560526776587366
1113	13	0.0350390133922005
1113	14	0.8695457522691683
1113	15	0.4579954427952515
1113	16	0.6024021101392663
1113	17	1.1051410587550592
1113	18	1.6757453708672614
1113	19	0.7844339006984029
1113	20	0.5291336446742108
1113	21	0.5100452376962963
1113	22	0.830221020317282
1113	23	0.436332620114527
1113	24	0.5825817653881629
1113	25	1.100465993006658
1114	1	-0.4442305876594155
1114	2	-3.356212411846375
1114	3	-1.2902027580811648
1114	4	0.005005167949189371
1114	5	0.15989624449272455
1114	6	0.008031884047674964
1114	7	-0.1908598255435976
1114	8	-0.15669395682088094
1114	9	-0.042015098285055785
1114	10	0.011498634797888485
1114	11	-0.1106415173987827
1114	12	-0.020499427468323006
1114	13	-0.15572710710898718
1114	14	0.4856823171468889
1114	15	0.07148933467485818
1114	16	0.2948921388971968
1114	17	0.6249914705340487
1114	18	0.986309402305082
1114	19	0.4170308509479739
1114	20	0.12216592093834322
1114	21	-0.06435315750183435
1114	22	-0.0013404095862554755
1114	23	1.621447064114932
1114	24	0.7435624732875823
1114	25	0.5192963604341969
1115	1	-0.6220921917655282
1115	2	-0.350181038812341
1115	3	-0.5202627420030019
1115	4	-0.37585923952921974
1115	5	-0.4789515901083057
1115	6	-0.8493089318161182
1115	7	-1.0405207411744613
1115	8	-0.9246504839373237
1115	9	-1.1400620135448776
1115	10	-0.7790987308566812
1115	11	-0.7710959316794899
1115	12	-1.165838821032935
1115	13	-1.122397896381969
1115	14	-0.35133044231964305
1115	15	-1.0951185848518676
1115	16	-0.6684578909917036
1115	17	-0.02015003792759984
1115	18	0.36179329322092
1115	19	-0.4185456955447151
1115	20	-0.9523934755120962
1115	21	-0.9564995924780249
1115	22	-0.46838068744992256
1115	23	1.1144046218829544
1115	24	-0.13828517579617028
1115	25	-0.33726322153351873
1116	1	-0.017935642282239428
1116	2	-0.10466133816366963
1116	3	0.025787157704922237
1116	4	0.7379634722278254
1116	5	0.23236088716468473
1116	6	-0.025669326415595606
1116	7	-0.10862610064238254
1116	8	-0.21065299417180938
1116	9	-4.8476518415441205E-4
1116	10	0.36962679728961945
1116	11	0.009276146067399793
1116	12	0.05970046882877256
1116	13	-0.17042431249439005
1116	14	0.6529568974183064
1116	15	-0.04412152909885947
1116	16	0.4240424902355002
1116	17	0.6118542787373116
1116	18	1.0286130372708442
1116	19	0.23368008479803848
1116	20	0.06033306142002459
1116	21	-0.056508390028788824
1116	22	0.4224804168186509
1116	23	1.8515021339355968
1116	24	0.6415872010674448
1116	25	0.9479715557711472
1201	1	0.8114773411431742
1201	2	1.252141148024176
1201	3	0.9007802516275731
1201	4	1.5733870449718452
1201	5	1.3560855648810808
1201	6	0.8336953144683861
1201	7	0.6346013395049468
1201	8	0.6906361402664984
1201	9	0.9056386233977918
1201	10	1.1861507383577532
1201	11	0.8865712657727371
1201	12	0.9584366566740741
1201	13	0.8856806598813023
1201	14	1.7179476893151744
1201	15	1.2860119883965382
1201	16	1.622343595207873
1201	17	1.9780214058091303
1201	18	2.5471144184863093
1201	19	1.617823731206876
1201	20	1.4552524905176298
1201	21	1.245517392659977
1201	22	1.6139468072422218
1201	23	3.2541816919466484
1201	24	2.0956134441613656
1201	25	2.329437946194716
1202	1	1.2157200730799005
1202	2	1.4728186099024718
1202	3	1.279442251057528
1202	4	1.7978326770083228
1202	5	1.4971405028887723
1202	6	1.1132927207673688
1202	7	0.9875473990745576
1202	8	1.127632007446202
1202	9	1.2789498675192164
1202	10	1.7884982515400267
1202	11	1.289495770546857
1202	12	1.433085901365537
1202	13	1.3507177127528962
1202	14	2.1943606863224403
1202	15	1.8019750067804665
1202	16	1.8536571907615311
1202	17	2.3758237073624997
1202	18	3.016865914852282
1202	19	2.0945645036167377
1202	20	1.8416343132338895
1202	21	1.6821356055714745
1202	22	2.200952115778634
1202	23	3.760460016016765
1202	24	2.5818164155166485
1202	25	2.6621351458526874
1203	1	0.9736801271916669
1203	2	1.2205331443378036
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2211	14	-0.16881785314723885
2211	15	-0.3522803724323893
2211	16	-0.5235493344954989
2211	17	0.11761523623037107
2211	18	-5.595002099189429
2211	19	-2.10996849417576
2211	20	-1.126115686375229
2211	21	-0.8403216971409151
2211	22	-0.6310777423988441
2211	23	-4.940529807443845
2211	24	-2.4014319642092126
2211	25	-1.6381275759507545
2212	1	0.3850413907299526
2212	2	0.6348718642776205
2212	3	0.30891730396917083
2212	4	0.9504256124649544
2212	5	0.9558582921538026
2212	6	0.545031402622655
2212	7	0.33467709708070004
2212	8	0.31763338544556063
2212	9	0.056671681249035544
2212	10	-3.541872677620205
2212	11	-0.9271925083319488
2212	12	-0.08808882541958951
2212	13	0.14252638990885202
2212	14	0.9429063670011217
2212	15	0.6373549636031512
2212	16	0.6802568183483686
2212	17	1.2459174113187714
2212	18	1.7907197903871364
2212	19	1.0955372298294677
2212	20	0.6401939505727299
2212	21	0.48436036786658576
2212	22	0.8095631984267939
2212	23	2.039842848971439
2212	24	1.2802931962274826
2212	25	1.198001995781496
2213	1	-1.284547291980516
2213	2	-3.4766455396068494
2213	3	-1.4931479454374177
2213	4	-3.332619244336332
2213	5	-1.175921772645129
2213	6	-0.7318497589068791
2213	7	-0.7317036919415969
2213	8	-0.5303282203205022
2213	9	-0.5022754227237982
2213	10	-0.6240746285185566
2213	11	-0.731151480558502
2213	12	-0.5990292762399747
2213	13	-0.9762375791124072
2213	14	-5.874945392445834
2213	15	-2.7442553072009446
2213	16	-1.8957329247082697
2213	17	-0.778050397125881
2213	18	0.1726306658657677
2213	19	-0.7333502729859944
2213	20	-0.9299335681825767
2213	21	-1.1751325025182169
2213	22	-0.8784195449590371
2213	23	-5.332705100506182
2213	24	-3.2813021742872692
2213	25	-2.5316495800107326
2214	1	-0.12172221934238436
2214	2	0.08624973599683017
2214	3	-0.07518476162762511
2214	4	0.21761545295286666
2214	5	-0.09638304123026487
2214	6	-0.35422361953468595
2214	7	-0.5202755266873247
2214	8	-0.4753468372694307
2214	9	-0.2904999282974785
2214	10	0.14590342427933933
2214	11	-0.44466792077672324
2214	12	-0.09190850169065357
2214	13	-0.2019550314818872
2214	14	0.2917568144721443
2214	15	-0.11856628703250749
2214	16	0.2981148384058656
2214	17	0.6005540205712379
2214	18	1.1907147742837196
2214	19	0.14744421397512042
2214	20	0.012658483262150355
2214	21	-0.0588986810110157
2214	22	0.29288551939514207
2214	23	1.6995291176477778
2214	24	0.7766688020517023
2214	25	0.6502798256421869
2215	1	-2.360651498447268
2215	2	-4.3672203423057425
2215	3	-2.5779469952438703
2215	4	-4.301789291774462
2215	5	-5.065612387337353
2215	6	-2.4301328994110243
2215	7	-1.8802934272754896
2215	8	-2.5369804695173457
2215	9	-1.9024671786499887
2215	10	-3.3195290113578295
2215	11	-2.1800399401430752
2215	12	-1.8141500896285443
2215	13	-1.7403375426215426
2215	14	-0.9997656212650341
2215	15	-1.5881774313986838
2215	16	-2.528006234143664
2215	17	-6.942824726159014
2215	18	-3.5940417248583962
2215	19	-2.3985528330707133
2215	20	-2.1046294776279915
2215	21	-1.954994552732515
2215	22	-1.7140807809261496
2215	23	-6.511279058906595
2215	24	-3.9495188083983592
2215	25	-3.6652676852046007
2216	1	-1.3842438361895084
2216	2	-0.67471624490857
2216	3	-1.0556476509941497
2216	4	-3.6135930885569003
2216	5	-1.529133047722734
2216	6	-1.3444914629294331
2216	7	-1.5568670695489573
2216	8	-1.293698658626191
2216	9	-1.5031180937492508
2216	10	-1.1476967620493426
2216	11	-1.6733007013335985
2216	12	-2.3582986063124096
2216	13	-1.8554779462089108
2216	14	-7.101744543220246
2216	15	-3.9575700829273472
2216	16	-2.233126605085239
2216	17	-1.4192634419355343
2216	18	-0.6134699733065574
2216	19	-1.1400056125482152
2216	20	-1.7025416391029538
2216	21	-2.0274430372374326
2216	22	-1.6363281886867789
2216	23	-0.5186541654055752
2216	24	-1.093774074158123
2216	25	-0.9960756102293189
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81187.0
3	1.0
4	658.0
5	4005.0
6	4931.0
7	4291.0
8	4053.0
9	3846.0
10	3770.0
11	3605.0
12	3477.0
13	3564.0
14	3535.0
15	3692.0
16	3824.0
17	4051.0
18	4654.0
19	5405.0
20	6755.0
21	8469.0
22	10446.0
23	12671.0
24	15128.0
25	17756.0
26	19816.0
27	22783.0
28	25120.0
29	30824.0
30	35756.0
31	40766.0
32	52053.0
33	66979.0
34	85758.0
35	111814.0
36	154425.0
37	210581.0
38	459594.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.746323830086524	17.868263081276478	12.092120974205473	25.29329211443152
2	14.285555483627551	19.306718664074644	41.64951837662419	24.758207475673622
3	20.011297913155136	23.651475070710028	28.23595639319353	28.101270622941303
4	11.159085336951016	15.783789084846603	38.44899560321014	34.60812997499224
5	12.861314193134163	37.65304161005724	35.88676195793881	13.59888223886979
6	33.516694350584956	35.56072844985093	17.597362739080214	13.325214460483902
7	29.471597862125947	30.715139265131942	20.896176092208893	18.917086780533214
8	26.081195217873354	36.43658609292304	19.667502562600674	17.814716126602935
9	26.777147698700453	15.196513630236124	19.291991312841017	38.7343473582224
10	14.394554567434367	29.064689029501107	34.362034178445015	22.178722224619516
11	35.194749926621085	22.548081983041463	22.82309765573163	19.434070434605825
12	23.647157954739335	24.742100236572902	30.86371008281686	20.747031725870904
13	29.311548492950106	21.09582235702473	24.996175995711887	24.59645315431328
14	21.098198824231577	21.42871617933443	27.770975529556658	29.702109466877335
15	24.468450211639347	29.322590661720245	22.572227034270867	23.636732092369545
16	22.593044623111506	27.76097371877131	27.535573433883126	22.11040822423406
17	22.665550953967045	27.857406687085078	27.577868356797076	21.899174002150797
18	22.34623578454412	26.69286247982528	29.455908471976993	21.504993263653603
19	23.713286731574392	26.955796322592963	27.43587027869526	21.89504666713739
20	23.777986532078977	26.83954922145323	27.654867690521527	21.727596555946263
21	23.249665603658944	26.47209572038059	27.305177357543236	22.97306131841723
22	23.104946880257135	27.309674141810003	26.633164843484895	22.952214134447967
23	21.776142197024573	26.726272819751095	28.752755629591082	22.744829353633246
24	22.806481858041973	27.479897844829278	27.378814963404857	22.33480533372389
25	23.015759382569552	26.490211269217067	27.15124888448677	23.342780463726616
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	128.0
1	128.0
2	233.5
3	339.0
4	339.0
5	339.0
6	664.0
7	989.0
8	989.0
9	989.0
10	1529.5
11	2070.0
12	2070.0
13	2070.0
14	3799.0
15	5528.0
16	5528.0
17	5528.0
18	9879.0
19	14230.0
20	14230.0
21	14230.0
22	22774.5
23	31319.0
24	31319.0
25	31319.0
26	45066.0
27	58813.0
28	58813.0
29	58813.0
30	76605.0
31	94397.0
32	94397.0
33	94397.0
34	111867.0
35	129337.0
36	129337.0
37	129337.0
38	144890.5
39	160444.0
40	160444.0
41	160444.0
42	174682.5
43	188921.0
44	188921.0
45	188921.0
46	207053.0
47	225185.0
48	225185.0
49	225185.0
50	215528.5
51	205872.0
52	205872.0
53	205872.0
54	182001.0
55	158130.0
56	158130.0
57	158130.0
58	135230.0
59	112330.0
60	112330.0
61	112330.0
62	92194.5
63	72059.0
64	72059.0
65	72059.0
66	55687.0
67	39315.0
68	39315.0
69	39315.0
70	28872.0
71	18429.0
72	18429.0
73	18429.0
74	12991.5
75	7554.0
76	7554.0
77	7554.0
78	5309.0
79	3064.0
80	3064.0
81	3064.0
82	2068.5
83	1073.0
84	1073.0
85	1073.0
86	721.5
87	370.0
88	370.0
89	370.0
90	248.0
91	126.0
92	126.0
93	126.0
94	70.5
95	15.0
96	15.0
97	15.0
98	10.5
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10574866196570945
2	0.04810322324274547
3	0.036404205633436444
4	0.03385525766269314
5	0.036404205633436444
6	0.01790799343547861
7	0.019541934442365346
8	0.022679101175587874
9	0.028038427678176363
10	0.03535848338902893
11	0.02039158376594645
12	0.018430854557682366
13	0.015228330184184367
14	0.0209144448881502
15	0.020783729607599263
16	0.0209144448881502
17	0.024705188024127425
18	0.018430854557682366
19	0.03182917081415359
20	0.021829451852006774
21	0.02751556655597261
22	0.037515285518119425
23	0.029214865203134814
24	0.03954137236665898
25	0.03124095205167436
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1530043.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.07948526658873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.61945646550853	70.07981001897284
2	8.291480263386612	13.113719826533856
3	1.7257142766716442	4.094057901491913
4	0.5479306963508729	1.7332030971676224
5	0.2561008680438706	1.0126162410617925
6	0.14358558949079586	0.681280470517911
7	0.08490770672215021	0.4700120419927881
8	0.054837987026967926	0.3469247829718795
9	0.04594046722181622	0.32696536507270363
>10	0.20622974226486443	2.8979281282702636
>50	0.012427124791780358	0.6711401238933546
>100	0.008374322477408066	1.3172041000183543
>500	0.0016747166903373605	0.8781813510880241
>1k	0.0011723016832361525	1.5097011479004572
>5k	1.6747166903373608E-4	0.8672554030462866
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	7165	0.4682874925737381	No Hit
CCCATGTACTCTGCGTTGATACCAC	5932	0.3877015221140844	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3255	0.21273911909665283	No Hit
GCGTTGATACCACTGCTTCCCATGT	1997	0.13051920763011235	No Hit
CATGTACTCTGCGTTGATACCACTG	1958	0.12797025965936903	No Hit
GTATCAACGCAGAGTACATGGGAAG	1952	0.12757811381771622	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1821	0.11901626294162976	No Hit
GTCCTACAGTGGACATTTCTAAATT	1569	0.10254613759221147	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.535764027546938E-5
2	0.0	0.0	0.0	0.0	6.535764027546938E-5
3	0.0	0.0	0.0	0.0	6.535764027546938E-5
4	0.0	0.0	0.0	0.0	6.535764027546938E-5
5	0.0	0.0	0.0	0.0	6.535764027546938E-5
6	0.0	0.0	0.0	0.0	6.535764027546938E-5
7	0.0	0.0	0.0	0.0	6.535764027546938E-5
8	0.0	0.0	0.0	0.0	1.3071528055093876E-4
9	0.0	0.0	0.0	0.0	1.9607292082640814E-4
10	0.0	6.535764027546938E-5	0.0	0.0	1.9607292082640814E-4
11	0.0	6.535764027546938E-5	0.0	6.535764027546938E-5	1.9607292082640814E-4
12	0.0	6.535764027546938E-5	0.0	6.535764027546938E-5	3.2678820137734693E-4
13	0.0	6.535764027546938E-5	0.0	6.535764027546938E-5	3.921458416528163E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGAC	100	6.5847416E-10	14.252574	3
GTGCGAA	40	0.0052853245	14.24745	11
TAGGACC	410	0.0	13.441893	4
CGTGCGC	65	5.4598662E-5	13.151922	10
TGTAGGA	940	0.0	12.029556	2
AGGACGT	585	0.0	12.018479	5
GTAGGAC	870	0.0	11.904449	3
GGACGTG	595	0.0	11.813398	6
CTGTAGG	945	0.0	11.473643	1
GTCCTAC	975	0.0	11.413256	1
TATAGGA	260	0.0	11.32971	2
TCCTACA	1190	0.0	11.259073	2
TAGGACG	640	0.0	11.135188	4
GACGTGA	300	0.0	11.081713	7
TAGACAG	250	0.0	11.021271	5
GGACCTG	640	0.0	10.982768	6
ATAGGAC	200	0.0	10.926973	3
TGGCGAG	665	0.0	10.856264	18
CCAACGA	80	3.7734205E-4	10.686635	19
TTAGACC	135	5.6668796E-8	10.557808	4
>>END_MODULE