FastQCFastQC Report
Thu 26 May 2016
SRR1513078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513078_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530043
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC86850.5676311057924516No Hit
CCCATGTACTCTGCGTTGATACCAC78890.5156064241331779No Hit
GAGTACATGGGAAGCAGTGGTATCA34440.22509171310871656No Hit
GTATCAACGCAGAGTACTTTTTTTT28100.18365496917406898No Hit
CATGTACTCTGCGTTGATACCACTG23170.15143365251826255No Hit
GTATCAACGCAGAGTACATGGGAAG22250.14542074961291937No Hit
GCGTTGATACCACTGCTTCCCATGT20690.13522495772994614No Hit
ACGCAGAGTACATGGGAAGCAGTGG20190.13195707571617268No Hit
TATCAACGCAGAGTACTTTTTTTTT18800.12287236371788243No Hit
TATCAACGCAGAGTACATGGGAAGC18290.1195391240638335No Hit
GCTTCCCATGTACTCTGCGTTGATA17720.11581373856813175No Hit
GGTATCAACGCAGAGTACTTTTTTT17070.11156549195022623No Hit
ACTCTGCGTTGATACCACTGCTTCC16080.10509508556295476No Hit
GTCCTACAGTGGACATTTCTAAATT15660.10235006467138505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGAA707.3759584E-613.5510510
TAGGACC3800.012.775114
AAGCCCG604.0245932E-412.6916135
CGAAATC604.1156844E-412.65798813
GACGAAA751.4985417E-512.65012611
GTAGGAC7850.011.883233
TCGAACT1201.0197255E-811.86026919
ACACCGT1052.6509952E-711.7850696
ACGAAAT658.0695504E-411.68429712
TGTAGGA9550.011.6666392
GTCCTAC9500.011.629711
CCGTCCT907.564013E-611.5967089
GGCGAGG3000.011.38585819
CGTGAAA3500.011.1147669
GATCGTG600.005928677511.0699457
TAGGACA4050.011.0464044
CTGTAGG8550.011.0281741
GTTCTAA1900.011.0281731
CCTAGAC1657.8216544E-1110.9609393
CCCCTAC2000.010.9529821
Produced by FastQC (version 0.11.5)