FastQCFastQC Report
Thu 26 May 2016
SRR1513077_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513077_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180104
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC89540.4107143512419591No Hit
CCCATGTACTCTGCGTTGATACCAC72020.33035121260270156No Hit
GAGTACATGGGAAGCAGTGGTATCA36750.16856993978268928No Hit
GCGTTGATACCACTGCTTCCCATGT25070.11499451402318421No Hit
GTATCAACGCAGAGTACATGGGAAG23630.10838932454598496No Hit
CATGTACTCTGCGTTGATACCACTG23070.10582063974929636No Hit
GTATCAACGCAGAGTACTTTTTTTT22690.10407760363725768No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGG551.9618664E-413.81723110
TCGAACT1850.011.29677919
CCGACCA700.001492676610.8563969
TTATACT3100.010.727534
TAGGACG5000.010.4516794
TAGGACC4000.010.4516794
GTCCTAA3650.010.4226771
TGGCGAG4650.010.41887718
TCTAGAC2750.010.3641123
TTAGGAC4050.010.3214613
GTATAGG2600.010.2422851
GTCCTAC7750.010.1856451
GTATAAC2151.8189894E-1210.174231
AGGACGT4950.010.172655
TTAGTAC1502.4439942E-810.1337993
GGCGAGA3200.010.093286519
GCCTTAC1701.5297701E-910.0701461
GACCTCG856.599383E-410.0581317
CTTAGAC2002.5465852E-119.9754583
CTCGAAC2002.5465852E-119.97454318
Produced by FastQC (version 0.11.5)