FastQCFastQC Report
Thu 26 May 2016
SRR1513077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513077_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180104
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC100880.46273021837490325No Hit
CCCATGTACTCTGCGTTGATACCAC96300.4417220462877No Hit
GAGTACATGGGAAGCAGTGGTATCA38370.17600077794453842No Hit
GTATCAACGCAGAGTACTTTTTTTT33850.15526782208555187No Hit
CATGTACTCTGCGTTGATACCACTG27720.12714989743608562No Hit
GTATCAACGCAGAGTACATGGGAAG27090.12426012703981093No Hit
GCGTTGATACCACTGCTTCCCATGT25490.11692102762070067No Hit
ACGCAGAGTACATGGGAAGCAGTGG23420.10742606774722674No Hit
TATCAACGCAGAGTACTTTTTTTTT22200.10183000444015514No Hit
TATCAACGCAGAGTACATGGGAAGC21870.10031631518496364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG1600.012.5055831
GCCTACG701.0616467E-412.2503651
GTAGGAC7300.011.8667853
TAGGACC4500.011.6349554
CTACACG1156.8796E-811.5889674
TAGGACG5300.011.4952564
CTAAGAC2250.011.423413
ATACCGA600.005787877411.1060926
GCCGCGT600.005934246411.0688938
CACCGTT600.005934246411.0688937
GTATTGA2100.010.88921451
TGGACTG4000.010.7094475
TGTAGGA8400.010.6591632
GGACGTG5750.010.5956286
AACACCG1105.901393E-610.3849185
TATACCG750.002603608410.1541425
CTATACT3200.010.1144784
GACGGTG856.674725E-410.0457187
TGGCGAG6350.010.01308918
TAAGACA3650.09.9107224
Produced by FastQC (version 0.11.5)