FastQCFastQC Report
Thu 26 May 2016
SRR1513076_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513076_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2081905
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC83820.40261203080832214No Hit
CCCATGTACTCTGCGTTGATACCAC65620.3151920957008125No Hit
GAGTACATGGGAAGCAGTGGTATCA35440.17022870880275517No Hit
GTATCAACGCAGAGTACATGGGAAG23020.11057180803158645No Hit
GCGTTGATACCACTGCTTCCCATGT22440.1077858980116768No Hit
CATGTACTCTGCGTTGATACCACTG22210.10668114058998848No Hit
GTATCAACGCAGAGTACTTTTTTTT20850.10014866192261415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAT1101.2732926E-1114.67957214
TGCGCAT602.573378E-514.247478510
GCGTCGT400.00528610414.24747811
TAGGACC4500.013.5135884
GCGTTTA1301.4551915E-1113.151834513
GTATTAC1600.012.4830361
TTGCGCG550.00306980312.09051218
GTCCTAC8700.012.0252561
GGCGAGA4150.011.90322319
TATACCG658.014704E-411.6941725
GTAGGAC8450.011.6938913
TCCTACA9750.011.2069132
TGTAGGA9000.011.0850992
TTTGCGC600.00587928211.08270317
TGCGTTT1553.1468517E-1011.03030612
TGGCGAG6150.010.81265218
TAGGACG4750.010.6019444
CTAATAC1803.45608E-1110.5569843
GTGTTAG3000.010.4619731
GCTACAC2750.010.3650393
Produced by FastQC (version 0.11.5)