##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513076_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2081905 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.482666115889053 31.0 30.0 34.0 25.0 34.0 2 29.204636618865894 31.0 30.0 34.0 22.0 34.0 3 29.557874638852397 31.0 30.0 34.0 25.0 34.0 4 32.43109988207915 35.0 33.0 37.0 19.0 37.0 5 32.66901611745013 35.0 33.0 37.0 25.0 37.0 6 33.18311882626729 37.0 35.0 37.0 27.0 37.0 7 33.208663699832606 37.0 35.0 37.0 27.0 37.0 8 33.1978836690435 37.0 35.0 37.0 27.0 37.0 9 34.60656177875551 39.0 35.0 39.0 27.0 39.0 10 34.09924756412997 39.0 34.0 39.0 25.0 39.0 11 34.49089607835132 39.0 35.0 39.0 27.0 39.0 12 34.32443459235652 39.0 35.0 39.0 26.0 39.0 13 34.420896246466576 39.0 35.0 39.0 27.0 39.0 14 34.73019037852352 40.0 34.0 41.0 18.0 41.0 15 35.292417761617365 40.0 35.0 41.0 26.0 41.0 16 34.877350311373476 40.0 34.0 41.0 22.0 41.0 17 34.40745278963257 39.0 33.0 41.0 17.0 41.0 18 33.803556838568525 39.0 32.0 41.0 11.0 41.0 19 34.714268422430415 39.0 34.0 41.0 21.0 41.0 20 34.97696917006299 40.0 34.0 41.0 24.0 41.0 21 35.002413174472416 40.0 35.0 41.0 23.0 41.0 22 34.59190981336805 40.0 34.0 41.0 18.0 41.0 23 32.881938897308 39.0 32.0 41.0 9.0 41.0 24 33.99561987698766 39.0 33.0 41.0 15.0 41.0 25 33.73196999863106 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.875869522349408 1101 2 1.3568069930580045 1101 3 1.0388073307794876 1101 4 1.1428771008701162 1101 5 1.3099023068036075 1101 6 1.0083651442498294 1101 7 0.9881455421641192 1101 8 0.9019660958623916 1101 9 1.078662788280127 1101 10 1.4458087463796971 1101 11 1.1356933523787518 1101 12 1.153618199945953 1101 13 1.0196998649226146 1101 14 0.13592837175190198 1101 15 0.6300173366954809 1101 16 1.388787465495028 1101 17 1.9643727120472292 1101 18 -4.000555833516071 1101 19 -0.44592589269287686 1101 20 0.5638211514366134 1101 21 0.9556626907380448 1101 22 1.3749252094651396 1101 23 3.3122910757442483 1101 24 2.2448063080641134 1101 25 2.438879376661255 1102 1 0.8276403735239661 1102 2 1.2132147231933104 1102 3 0.8869184805008352 1102 4 1.5100249727690382 1102 5 1.3889077711584932 1102 6 0.8986663260524423 1102 7 0.8354530134095555 1102 8 0.6662906765662058 1102 9 0.8585090271307365 1102 10 1.2364297115184968 1102 11 0.8559053719832335 1102 12 0.920888641046858 1102 13 0.8769561510716812 1102 14 1.7608421163007222 1102 15 1.275192672000685 1102 16 1.4382676271529746 1102 17 1.0619333914190392 1102 18 1.4775624554392834 1102 19 1.214932495207151 1102 20 1.1000132388267616 1102 21 1.1463691702310683 1102 22 1.4581524561417325 1102 23 3.104694996641207 1102 24 1.9441294806837135 1102 25 2.222117986824493 1103 1 0.9417335445761701 1103 2 1.275781540460759 1103 3 1.0476835635610868 1103 4 1.5259551624124654 1103 5 1.332944507474636 1103 6 0.8913060846585878 1103 7 0.9147152250509691 1103 8 0.8672186153092696 1103 9 0.9346589127606606 1103 10 1.245894139181452 1103 11 0.953997176272523 1103 12 1.1152157637878446 1103 13 0.9707141610324186 1103 14 1.8342238000553053 1103 15 1.2736846701512974 1103 16 1.5058402643159496 1103 17 1.9891460670298216 1103 18 2.087334810327185 1103 19 1.4833205260951772 1103 20 1.3498091369157308 1103 21 1.3362133511466752 1103 22 1.569687407960302 1103 23 3.0520701629239326 1103 24 1.9882892053844046 1103 25 2.2483941053800223 1104 1 0.8054817146909272 1104 2 1.2053103979926973 1104 3 0.8642610721212058 1104 4 1.4928102583051412 1104 5 1.2398824804996664 1104 6 0.8200707156848139 1104 7 0.7594683807819465 1104 8 0.8590705013118196 1104 9 0.8436764980012157 1104 10 1.2830416151667663 1104 11 0.8332607902964924 1104 12 1.0327022041708958 1104 13 0.8464913634260327 1104 14 1.7174565733557614 1104 15 1.239862689914304 1104 16 1.548811645976599 1104 17 2.089441079480231 1104 18 2.664414757164458 1104 19 1.7199633429063752 1104 20 1.2496918953688976 1104 21 1.355815741188728 1104 22 1.7176957145114997 1104 23 3.155837904693108 1104 24 2.1992188936706114 1104 25 2.1800685080058386 1105 1 0.6763918314775381 1105 2 1.0430084028450572 1105 3 0.6935326900509899 1105 4 1.0467946814332691 1105 5 1.0946023147473198 1105 6 0.7917274230708884 1105 7 0.5383629055050605 1105 8 0.6377880825242173 1105 9 0.8335049241466308 1105 10 1.279927915765839 1105 11 0.7651258528051699 1105 12 0.7885227887096704 1105 13 0.7489233287922374 1105 14 1.525289201349608 1105 15 0.8688488610534435 1105 16 1.3259785125689802 1105 17 1.755858545022015 1105 18 1.6337303957571976 1105 19 1.2510315850617033 1105 20 1.12149753913652 1105 21 0.9507954535488921 1105 22 1.431929607539459 1105 23 2.8727449399164513 1105 24 1.9013865441899398 1105 25 1.7971798900992368 1106 1 -0.6476362047770117 1106 2 -0.23280432943414198 1106 3 -0.48636627674393296 1106 4 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0.8433463607533653 2212 20 0.42574978344460845 2212 21 0.24085696534911705 2212 22 0.3351782664658671 2212 23 1.7008397045243555 2212 24 0.9120604030526636 2212 25 0.7426011801018078 2213 1 -1.4157428684746094 2213 2 -3.8630442670837546 2213 3 -1.6205727010605813 2213 4 -3.355727968793378 2213 5 -1.24045809796932 2213 6 -0.8121669968664378 2213 7 -0.8845117675119951 2213 8 -0.6294027385830745 2213 9 -0.585191022546212 2213 10 -0.670710138450886 2213 11 -0.747454695252479 2213 12 -0.6513831105809231 2213 13 -1.0245216208392876 2213 14 -6.010230163652288 2213 15 -2.670290983035123 2213 16 -1.8582189788182504 2213 17 -0.740274459725093 2213 18 0.006902705011249566 2213 19 -0.6897947814965519 2213 20 -0.7561120611094978 2213 21 -0.9124253671797291 2213 22 -0.828638553893633 2213 23 -5.61523639293851 2213 24 -3.0686383139724214 2213 25 -2.1551701988110565 2214 1 -0.15521943332516486 2214 2 0.031658898439712146 2214 3 -0.14496946543157563 2214 4 0.15553637526540598 2214 5 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-2.7747361423859687 2215 17 -7.236970327967246 2215 18 -3.4811691454455236 2215 19 -2.674380501539545 2215 20 -2.3518461785492946 2215 21 -2.3989700882742966 2215 22 -2.065120917058799 2215 23 -6.596858412269203 2215 24 -4.115355568380593 2215 25 -3.782891948109409 2216 1 -1.1912769858448726 2216 2 -0.6306168044471505 2216 3 -0.8423919831833757 2216 4 -3.5639445285711204 2216 5 -1.5098738326339642 2216 6 -1.2395259713089537 2216 7 -1.2051005137279844 2216 8 -1.1162946622458847 2216 9 -1.1459480255727357 2216 10 -0.6522088001028976 2216 11 -1.3177793987606847 2216 12 -2.062400246476784 2216 13 -1.436775157399623 2216 14 -7.388706811713497 2216 15 -3.588588392441334 2216 16 -1.933447871564013 2216 17 -1.036530120630978 2216 18 -0.2390026395149718 2216 19 -0.7730585637199212 2216 20 -1.3517580272965972 2216 21 -1.5382483410131371 2216 22 -1.0462136034997087 2216 23 0.11382037740118278 2216 24 -0.5959677416972582 2216 25 -0.6421994812716747 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 83968.0 3 0.0 4 704.0 5 4259.0 6 5374.0 7 4710.0 8 4488.0 9 4243.0 10 4213.0 11 4084.0 12 3977.0 13 3904.0 14 4123.0 15 4172.0 16 4380.0 17 4615.0 18 5196.0 19 6259.0 20 7773.0 21 9694.0 22 12013.0 23 14531.0 24 17552.0 25 20700.0 26 23928.0 27 27761.0 28 30794.0 29 37467.0 30 43994.0 31 50622.0 32 65241.0 33 85647.0 34 112400.0 35 148294.0 36 210033.0 37 292887.0 38 717905.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.3204289472558 17.33172148722617 11.936653293921964 25.41119627159607 2 14.600301688117534 19.00731250093106 41.04611484043159 25.346270970519814 3 20.066058646508882 23.05810979439078 28.19173344365531 28.68409811544503 4 11.120672672672672 15.461045045045044 37.94296696696697 35.475315315315314 5 13.075930664168078 37.239924561846074 35.70305236224729 13.98109241173856 6 34.2480462322441 35.03277218109518 17.250903318822903 13.468278267837814 7 29.89786113726771 30.355372139054904 20.62119261294848 19.125574110728905 8 26.528438512220877 35.77541103794588 19.403028098843205 18.29312235099004 9 26.73519405335777 15.1370603788289 18.938466128768 39.189279439045336 10 14.549891719925792 28.738257245779668 34.003450931852505 22.70840010244203 11 35.83222714980343 22.27120977803785 22.421151093860573 19.475411978298144 12 23.63346623498851 24.320714642258498 30.568549725701168 21.47726939705182 13 29.476088264061968 20.7035836377507 24.917466222643274 24.902861875544062 14 21.344207730712615 20.83480665531568 27.446979625878708 30.374005988092996 15 24.849312936170946 28.610219912311802 22.433803867082144 24.106663284435104 16 23.20936040229667 27.378138979143856 26.623856745949574 22.7886438726099 17 22.78009437094286 27.350613372692195 27.3122250296801 22.557067226684847 18 22.415646883383424 26.637674586641864 28.714650288639664 22.232028241335044 19 24.03718918918919 26.61929129129129 26.971051051051052 22.37246846846847 20 24.08660650884447 26.462779629617174 27.007841784479204 22.442772077059153 21 23.736290029654086 26.091560596771686 26.92160597156052 23.250543402013708 22 23.54736255393575 26.502320798777617 26.43466687167857 23.51564977560807 23 21.93568070493217 26.11520970915302 28.485851628882426 23.463257957032386 24 23.287047225668562 26.924474103773267 26.685467494812876 23.103011175745298 25 23.428122530071565 26.006721808882492 26.74309380983383 23.822061851212112 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 170.0 1 170.0 2 288.0 3 406.0 4 406.0 5 406.0 6 729.5 7 1053.0 8 1053.0 9 1053.0 10 1748.5 11 2444.0 12 2444.0 13 2444.0 14 4435.0 15 6426.0 16 6426.0 17 6426.0 18 12050.5 19 17675.0 20 17675.0 21 17675.0 22 28123.5 23 38572.0 24 38572.0 25 38572.0 26 55321.0 27 72070.0 28 72070.0 29 72070.0 30 94668.0 31 117266.0 32 117266.0 33 117266.0 34 139583.5 35 161901.0 36 161901.0 37 161901.0 38 185360.5 39 208820.0 40 208820.0 41 208820.0 42 229829.5 43 250839.0 44 250839.0 45 250839.0 46 276075.0 47 301311.0 48 301311.0 49 301311.0 50 292872.0 51 284433.0 52 284433.0 53 284433.0 54 256390.0 55 228347.0 56 228347.0 57 228347.0 58 198590.0 59 168833.0 60 168833.0 61 168833.0 62 139537.5 63 110242.0 64 110242.0 65 110242.0 66 86090.0 67 61938.0 68 61938.0 69 61938.0 70 45789.0 71 29640.0 72 29640.0 73 29640.0 74 20912.0 75 12184.0 76 12184.0 77 12184.0 78 8503.0 79 4822.0 80 4822.0 81 4822.0 82 3287.0 83 1752.0 84 1752.0 85 1752.0 86 1161.0 87 570.0 88 570.0 89 570.0 90 360.0 91 150.0 92 150.0 93 150.0 94 87.0 95 24.0 96 24.0 97 24.0 98 20.5 99 17.0 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11057180803158645 2 0.04553521894610945 3 0.034775842317492874 4 0.031461570052427945 5 0.03506403990575939 6 0.01988563359038957 7 0.02041399583554485 8 0.02243137895341046 9 0.02862762710114054 10 0.034967974043003884 11 0.020365962904167097 12 0.019981699453145076 13 0.01599496614879161 14 0.02118252273758889 15 0.021086456874833386 16 0.02190301670825518 17 0.02612991466949741 18 0.02002973238452283 19 0.031461570052427945 20 0.02358416930647652 21 0.027522869679452232 22 0.03482387524887063 23 0.027378770885318975 24 0.0379940487198023 25 0.029780417454206606 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2081905.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.29094249258583 #Duplication Level Percentage of deduplicated Percentage of total 1 85.02016361963231 63.162280861763406 2 10.246146766379653 15.223918003834097 3 2.5006029777314693 5.573164560463123 4 0.9048708564960283 2.688948350526532 5 0.4281675310064726 1.5904484711597155 6 0.23662836227209436 1.0547606432202556 7 0.15207487408539375 0.7908450007671652 8 0.1004259958565441 0.5968593506351343 9 0.07314613942980863 0.48906860741411196 >10 0.31014323345692957 3.9584882836703414 >50 0.01558994268094118 0.7874837012585598 >100 0.00909790687832764 1.2491095324428907 >500 0.0018958228605389731 0.9169330272438129 >1k 9.152248292255361E-4 1.1919158026808405 >5k 1.3074640417507657E-4 0.7257758029199934 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 8382 0.40261203080832214 No Hit CCCATGTACTCTGCGTTGATACCAC 6562 0.3151920957008125 No Hit GAGTACATGGGAAGCAGTGGTATCA 3544 0.17022870880275517 No Hit GTATCAACGCAGAGTACATGGGAAG 2302 0.11057180803158645 No Hit GCGTTGATACCACTGCTTCCCATGT 2244 0.1077858980116768 No Hit CATGTACTCTGCGTTGATACCACTG 2221 0.10668114058998848 No Hit GTATCAACGCAGAGTACTTTTTTTT 2085 0.10014866192261415 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.803293137775259E-5 2 0.0 0.0 0.0 0.0 4.803293137775259E-5 3 0.0 0.0 0.0 0.0 4.803293137775259E-5 4 0.0 0.0 0.0 0.0 4.803293137775259E-5 5 0.0 0.0 0.0 0.0 4.803293137775259E-5 6 0.0 0.0 0.0 0.0 4.803293137775259E-5 7 0.0 0.0 0.0 0.0 4.803293137775259E-5 8 0.0 0.0 0.0 0.0 4.803293137775259E-5 9 0.0 0.0 0.0 0.0 4.803293137775259E-5 10 0.0 0.0 0.0 4.803293137775259E-5 4.803293137775259E-5 11 0.0 0.0 0.0 4.803293137775259E-5 4.803293137775259E-5 12 0.0 0.0 0.0 4.803293137775259E-5 4.803293137775259E-5 13 0.0 0.0 0.0 4.803293137775259E-5 9.606586275550518E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTAT 110 1.2732926E-11 14.679572 14 TGCGCAT 60 2.573378E-5 14.2474785 10 GCGTCGT 40 0.005286104 14.247478 11 TAGGACC 450 0.0 13.513588 4 GCGTTTA 130 1.4551915E-11 13.1518345 13 GTATTAC 160 0.0 12.483036 1 TTGCGCG 55 0.003069803 12.090512 18 GTCCTAC 870 0.0 12.025256 1 GGCGAGA 415 0.0 11.903223 19 TATACCG 65 8.014704E-4 11.694172 5 GTAGGAC 845 0.0 11.693891 3 TCCTACA 975 0.0 11.206913 2 TGTAGGA 900 0.0 11.085099 2 TTTGCGC 60 0.005879282 11.082703 17 TGCGTTT 155 3.1468517E-10 11.030306 12 TGGCGAG 615 0.0 10.812652 18 TAGGACG 475 0.0 10.601944 4 CTAATAC 180 3.45608E-11 10.556984 3 GTGTTAG 300 0.0 10.461973 1 GCTACAC 275 0.0 10.365039 3 >>END_MODULE