FastQCFastQC Report
Thu 26 May 2016
SRR1513076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513076_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2081905
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC94260.4527584111666959No Hit
CCCATGTACTCTGCGTTGATACCAC87210.41889519454538027No Hit
GAGTACATGGGAAGCAGTGGTATCA37940.1822369416471933No Hit
GTATCAACGCAGAGTACTTTTTTTT31470.1511596350457874No Hit
CATGTACTCTGCGTTGATACCACTG25940.1245974239938902No Hit
GCGTTGATACCACTGCTTCCCATGT24440.11739248428722733No Hit
GTATCAACGCAGAGTACATGGGAAG24330.11686412204207206No Hit
ACGCAGAGTACATGGGAAGCAGTGG22770.10937098474714263No Hit
TATCAACGCAGAGTACTTTTTTTTT21610.10379916470732334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTG456.7979796E-414.76792414
TAGGACC5000.012.564264
CAGTGCG802.9063256E-511.8593849
GTAGGAC7650.011.82020953
ACCGTCC752.0999646E-411.3850098
TTAGGAC4600.011.380673
CCGTGCG600.0059346311.0687589
TAGGACG5950.010.8781474
TATGGCG6700.010.76893316
GTCCTAC8350.010.6107691
ACAACCG909.333006E-510.5759755
GACAACG909.643173E-510.5419287
CGTGCGC1803.6379788E-1110.5388910
TGGCGAG6950.010.51563418
AAGGCGT2500.010.2798496
TCCTACA9500.010.2268822
ATGGCGA7400.010.13488517
TTTAGGA5650.010.1150832
GCGTGCG1606.3082553E-910.0804769
TGTAGGA9650.010.0679142
Produced by FastQC (version 0.11.5)