FastQCFastQC Report
Thu 26 May 2016
SRR1513075_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513075_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2393942
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC87210.3642945401350576No Hit
CCCATGTACTCTGCGTTGATACCAC73480.30694143801311813No Hit
GAGTACATGGGAAGCAGTGGTATCA34830.1454922466793264No Hit
GTATCAACGCAGAGTACATGGGAAG24730.10330241918977151No Hit
GCGTTGATACCACTGCTTCCCATGT24570.10263406548696669No Hit
GTATCAACGCAGAGTACTTTTTTTT24030.10037837174000039No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGC500.001498058913.3022383
CGTAGAC655.4462813E-513.156063
AACCGTC604.1006453E-412.6640347
CGGAACA550.003065411212.0929454
GTCCTAC11000.011.84618951
GCGTTTA2100.011.76068913
GTATTAA3700.011.3110191
GTCTTAC1850.011.3110191
CGTTTAT2100.011.30835414
TAGGACC5800.011.3036264
GACGTGA3900.011.2027997
TAAGACG600.00586987311.0851984
TAACCCG600.0058744411.084045
GTAGGAC10150.011.0461933
TCCTACA12800.010.6897462
TCTAATA2400.010.6897452
TGCGTTT2450.010.46786812
TTAGACT1553.6543497E-910.4211094
CTAGGAC3750.010.3884143
GTGTAGG4500.010.357011
Produced by FastQC (version 0.11.5)