##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513075_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2393942
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.603005837234154	31.0	30.0	34.0	25.0	34.0
2	29.33334642192668	31.0	30.0	34.0	22.0	34.0
3	29.71337567911002	31.0	30.0	34.0	25.0	34.0
4	32.595785946359605	35.0	33.0	37.0	22.0	37.0
5	32.859307786070005	37.0	33.0	37.0	26.0	37.0
6	33.31005220677861	37.0	35.0	37.0	28.0	37.0
7	33.29733928390913	37.0	35.0	37.0	28.0	37.0
8	33.2968605755695	37.0	35.0	37.0	28.0	37.0
9	34.72379071840504	39.0	35.0	39.0	27.0	39.0
10	34.2228341371679	39.0	35.0	39.0	25.0	39.0
11	34.61079549964034	39.0	35.0	39.0	27.0	39.0
12	34.453248240767735	39.0	35.0	39.0	27.0	39.0
13	34.54784702386274	39.0	35.0	39.0	27.0	39.0
14	34.89391639396443	40.0	34.0	41.0	18.0	41.0
15	35.45229124180953	40.0	35.0	41.0	27.0	41.0
16	35.052043031953154	40.0	35.0	41.0	23.0	41.0
17	34.580590924926334	40.0	34.0	41.0	17.0	41.0
18	34.0021863520503	39.0	33.0	41.0	16.0	41.0
19	34.896332492600074	40.0	34.0	41.0	22.0	41.0
20	35.152116885037316	40.0	35.0	41.0	25.0	41.0
21	35.17860040051096	40.0	36.0	41.0	24.0	41.0
22	34.77476313127052	40.0	35.0	41.0	18.0	41.0
23	33.098553766131346	39.0	32.0	41.0	10.0	41.0
24	34.222722605643746	39.0	34.0	41.0	17.0	41.0
25	33.96700588401891	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7342463601596947
1101	2	1.039396561677421
1101	3	0.8542774463197951
1101	4	0.7872555046829035
1101	5	1.1374885704308895
1101	6	0.6812639993502998
1101	7	0.7318384067779036
1101	8	0.6826584238994826
1101	9	0.8108579034613825
1101	10	1.0700939518069958
1101	11	0.7757104319495554
1101	12	0.7988830208910898
1101	13	0.7109747463722087
1101	14	-0.032256855196315826
1101	15	0.5439659458356161
1101	16	1.1060013924013177
1101	17	1.7098873817237887
1101	18	-4.030371011546226
1101	19	-0.6338633680264607
1101	20	0.21873691711040522
1101	21	0.6011370145759471
1101	22	1.07111914086056
1101	23	2.867068428468727
1101	24	1.8111684593739952
1101	25	2.07990101258099
1102	1	1.0761609098768723
1102	2	1.3094504253173547
1102	3	1.147869911975718
1102	4	1.7038848708450374
1102	5	1.522650079670754
1102	6	1.0913026502964556
1102	7	1.0843045263290136
1102	8	1.001702513484794
1102	9	1.1775053714533499
1102	10	1.5300542911412336
1102	11	1.109172197259916
1102	12	1.213602787918127
1102	13	1.0380156907354134
1102	14	1.9596208140845732
1102	15	1.3709113635678207
1102	16	1.6669480053054713
1102	17	1.4324761005265287
1102	18	1.8337495903586785
1102	19	1.5624983897693454
1102	20	1.4770433388492776
1102	21	1.435725340692663
1102	22	1.7435657132359097
1102	23	3.2562871746521367
1102	24	2.1049977190566054
1102	25	2.4438351508584333
1103	1	0.747879009650255
1103	2	0.9695967634426808
1103	3	0.7477927592606939
1103	4	1.2293466415296308
1103	5	1.0649461420259172
1103	6	0.7442230432361043
1103	7	0.573657421106212
1103	8	0.5349342392433201
1103	9	0.69894751776215
1103	10	1.0580089758736406
1103	11	0.6496499544880763
1103	12	0.866504163805665
1103	13	0.711338631135213
1103	14	1.557970744951099
1103	15	1.0834539571821225
1103	16	1.2796879638437204
1103	17	1.7184568213598652
1103	18	1.800666869695192
1103	19	1.1514418298486717
1103	20	1.0014944563187527
1103	21	0.9701329159864471
1103	22	1.3433761384296972
1103	23	2.8718412750340647
1103	24	1.7525388065814838
1103	25	1.9763339181868673
1104	1	0.931084033985865
1104	2	1.3564229226844873
1104	3	1.1525096897791265
1104	4	1.6750481544726554
1104	5	1.4390833642340723
1104	6	1.10018978838918
1104	7	0.9662202614701698
1104	8	1.0527962261429664
1104	9	1.1942029180729605
1104	10	1.5724573767884777
1104	11	1.137022805907641
1104	12	1.1638687807835666
1104	13	1.0861760220107755
1104	14	2.020208355009892
1104	15	1.4532673439971262
1104	16	1.7853113159004579
1104	17	2.2214714950625805
1104	18	2.8479321730373144
1104	19	1.9201574016239533
1104	20	1.4347398692042717
1104	21	1.5838550162392693
1104	22	1.8241211986689194
1104	23	3.342619405659079
1104	24	2.264690786886341
1104	25	2.2919311020801487
1105	1	0.5349478923607371
1105	2	0.9209742753473797
1105	3	0.617438535560872
1105	4	0.8512753005011575
1105	5	0.8497543772133014
1105	6	0.697489875688035
1105	7	0.5098078587828709
1105	8	0.4686156860577384
1105	9	0.6427386194337004
1105	10	1.1827746174110345
1105	11	0.6071345722125159
1105	12	0.6143940482362567
1105	13	0.6067031363675852
1105	14	1.3644560261723555
1105	15	0.820157560877071
1105	16	1.180967907917875
1105	17	1.536140167945348
1105	18	1.4684748665217882
1105	19	1.0995143093167385
1105	20	0.8490842996587915
1105	21	0.6505871533831211
1105	22	1.1696011172173684
1105	23	2.7361906390198882
1105	24	1.5787793499729332
1105	25	1.6621417122289088
1106	1	-0.4693057763749131
1106	2	-0.0354917161264936
1106	3	-0.31017652716968414
1106	4	-2.7717686624841846
1106	5	-0.4679095773345381
1106	6	-0.3699701502784123
1106	7	-0.3732837155250621
1106	8	-0.2238108203155562
1106	9	-0.2206891132111437
1106	10	-0.04643252705399448
1106	11	-0.12839208356662368
1106	12	-0.14192004446223905
1106	13	-0.130052158137687
1106	14	0.5717627907572904
1106	15	-0.020426397964236287
1106	16	-0.9000570026971602
1106	17	-5.706326273440062
1106	18	-6.629535055351759
1106	19	-2.6413783663645845
1106	20	-1.362970799635768
1106	21	-0.9167827349297824
1106	22	-1.335555148629311
1106	23	-5.000095362687588
1106	24	-2.155826991856422
1106	25	-0.5687093625396216
1107	1	0.5627014154763685
1107	2	0.948105526032915
1107	3	0.691310607404187
1107	4	1.043832775413776
1107	5	0.925369330061379
1107	6	0.5082089393342883
1107	7	0.506168795793009
1107	8	0.44259165710069226
1107	9	0.4531327281849826
1107	10	0.8939251261543717
1107	11	0.5213147401612943
1107	12	0.6151821448253756
1107	13	0.5100403907513424
1107	14	1.2483521252453826
1107	15	0.7271733910385834
1107	16	-5.689215310504501
1107	17	-0.9545963504209709
1107	18	0.8144540193157965
1107	19	0.7250578800463572
1107	20	0.6107686412807425
1107	21	0.7695817494156003
1107	22	0.9048695407564509
1107	23	2.735713246812068
1107	24	1.6262629251763485
1107	25	1.3684012719484926
1108	1	0.4897903600531208
1108	2	0.7348851372669927
1108	3	0.5185883963253772
1108	4	1.071727680674826
1108	5	0.7640332534048184
1108	6	0.45676272391450823
1108	7	0.39425385049159445
1108	8	0.4961687946723998
1108	9	0.5212058151506085
1108	10	0.9215466041912919
1108	11	0.6248875274226506
1108	12	0.5708412416600694
1108	13	0.48005762849113154
1108	14	1.0830790907170567
1108	15	0.6998718359924112
1108	16	1.030758643107177
1108	17	1.1439458430638965
1108	18	1.036376488610344
1108	19	0.9136585779062116
1108	20	0.6647412483399862
1108	21	0.7976083318555922
1108	22	1.1161754280110046
1108	23	2.8059156501103573
1108	24	1.5079584250576588
1108	25	1.6752306314631085
1109	1	0.3146253754464574
1109	2	0.7131513887581953
1109	3	0.4344750597274398
1109	4	0.4602828510349113
1109	5	0.26533126375651506
1109	6	0.23088539317310364
1109	7	0.19720181221192234
1109	8	0.18758227482235412
1109	9	0.3041013016243497
1109	10	0.7638458446880207
1109	11	0.35837633974045247
1109	12	0.41596853033514947
1109	13	0.30346932417188555
1109	14	-0.7150603466842185
1109	15	-0.0023926868033257165
1109	16	-0.28606801116338687
1109	17	0.8239448060209114
1109	18	1.5234065158704837
1109	19	0.7725437523382155
1109	20	0.5829616941943954
1109	21	0.4480769874128754
1109	22	0.3325090583927661
1109	23	-4.614971586642849
1109	24	-1.5569816715485558
1109	25	-0.3011777299211005
1110	1	0.5932152171697602
1110	2	0.8603001814631064
1110	3	0.7035258049423021
1110	4	1.2109371644077385
1110	5	0.8985539566420684
1110	6	0.5203876364327016
1110	7	0.44836341726929874
1110	8	0.37557600095860266
1110	9	0.469806638349759
1110	10	0.7409082091908914
1110	11	0.4391134863570443
1110	12	0.5967997877231852
1110	13	0.3451916723412012
1110	14	1.2024096725742837
1110	15	0.659970422562516
1110	16	1.0518160504469307
1110	17	1.363933649545075
1110	18	2.017390239183129
1110	19	1.12343075269321
1110	20	0.691522044310112
1110	21	0.582439467872149
1110	22	0.8235496735388281
1110	23	2.3739412608146537
1110	24	1.3144524015053847
1110	25	1.714395730672443
1111	1	0.5100578782046092
1111	2	0.7787267918115752
1111	3	0.5670421724185459
1111	4	1.0863331517361416
1111	5	0.7744779270750186
1111	6	0.370440434743152
1111	7	0.34303078791344177
1111	8	0.09970482883610998
1111	9	0.2543895316971714
1111	10	0.46292510465023895
1111	11	0.3163574145513408
1111	12	0.45424434949720194
1111	13	0.31852852556190214
1111	14	-0.5248870539304633
1111	15	2.5553343896689285E-4
1111	16	0.48977803566340583
1111	17	1.1118217171269151
1111	18	1.7533986869128029
1111	19	0.8731667611409364
1111	20	0.7010257401918452
1111	21	0.5705598480199825
1111	22	0.8552286598112673
1111	23	0.6160215142465404
1111	24	0.6454978080523759
1111	25	0.3260641572827865
1112	1	0.24893242135984295
1112	2	0.5653996757805935
1112	3	0.37047707097016414
1112	4	0.9267914510067925
1112	5	0.6924414137175674
1112	6	0.07868767987469738
1112	7	-0.02489160271015578
1112	8	0.06963407965209711
1112	9	0.2030352398541666
1112	10	0.5103697547434436
1112	11	0.2456372102690736
1112	12	0.1990470416866401
1112	13	0.11115301834760061
1112	14	0.4816113058167417
1112	15	0.3127014724402173
1112	16	0.6156560525224322
1112	17	0.7082911026627698
1112	18	0.922996630400668
1112	19	0.3605193164080447
1112	20	0.3301325131138455
1112	21	0.36685731474987193
1112	22	0.3434769647729823
1112	23	2.092422629055406
1112	24	0.9433462424016952
1112	25	1.0112254584672016
1113	1	0.2827359967671086
1113	2	0.7282927745525853
1113	3	0.3647345724912512
1113	4	0.5072701161795123
1113	5	0.5810924842616458
1113	6	0.17879798533432023
1113	7	0.05553993024383885
1113	8	-0.09342960561608749
1113	9	0.001195396204593635
1113	10	0.2672600298670602
1113	11	-0.01372750633775155
1113	12	0.07794575990453012
1113	13	-0.12206255585136461
1113	14	0.6123141703844581
1113	15	0.19498500701384813
1113	16	0.419503809028825
1113	17	0.8102865629731539
1113	18	1.2841382852128689
1113	19	0.4490326858903657
1113	20	0.14418560005993442
1113	21	0.05783037011737946
1113	22	0.29061483401106614
1113	23	0.20993623848356435
1113	24	0.1574956290401417
1113	25	0.5447032591921825
1114	1	-0.31931884679737266
1114	2	-2.9955918282136764
1114	3	-1.0506568678195372
1114	4	0.22711585482178975
1114	5	0.40571487005954765
1114	6	0.1317112974554675
1114	7	0.13278771442114135
1114	8	0.15736917112480597
1114	9	0.1982617745452515
1114	10	0.46132619469718605
1114	11	0.13986708663827585
1114	12	0.2621853629832458
1114	13	0.17541645762583613
1114	14	1.046863233541913
1114	15	0.3365323334454047
1114	16	0.8245641387724021
1114	17	1.1349575744923115
1114	18	1.309592190372534
1114	19	0.7081282995508715
1114	20	0.41877127246187484
1114	21	0.25227063403722383
1114	22	0.5140769723450589
1114	23	2.0692781703893317
1114	24	1.0165103951159153
1114	25	1.004969991127112
1115	1	0.15074063259229575
1115	2	0.3177840393341498
1115	3	0.22478101593201316
1115	4	0.3139618464538927
1115	5	0.25086215670257417
1115	6	-0.025069061636258994
1115	7	-0.1516216039205247
1115	8	-0.17957255930636506
1115	9	-0.19894721776267232
1115	10	-0.012226366239652009
1115	11	0.0021183394383257337
1115	12	-0.1505193245202534
1115	13	-0.14127033003571654
1115	14	0.668262175725026
1115	15	-0.10563294812206436
1115	16	0.41428030948935657
1115	17	0.8563591738632894
1115	18	1.3192285622162032
1115	19	0.5230757176446588
1115	20	0.05347574675005973
1115	21	0.08387868409992194
1115	22	0.43480511865558924
1115	23	2.0950473222309682
1115	24	0.8345208144937999
1115	25	0.538044584793461
1116	1	0.27076722421957555
1116	2	0.20817250172490986
1116	3	0.23472555599703782
1116	4	0.9763696029522961
1116	5	0.32624759869730724
1116	6	0.16143052704347838
1116	7	0.011956366312638522
1116	8	0.041222943187356975
1116	9	0.10377842403354265
1116	10	0.49126606152127295
1116	11	0.09665218153384103
1116	12	0.15497172509012813
1116	13	-0.039402541420940906
1116	14	0.797422809188582
1116	15	0.07825604701191224
1116	16	0.4606669141088915
1116	17	0.8546531813368787
1116	18	1.3424142755912456
1116	19	0.4973461933494434
1116	20	0.3334558333853366
1116	21	0.10143037233434171
1116	22	0.526825505569839
1116	23	1.7867362244763783
1116	24	0.7543877349344399
1116	25	0.9029873490477556
1201	1	0.9649268302845755
1201	2	1.3213214918726557
1201	3	1.0874071484995618
1201	4	1.5635596218140222
1201	5	1.3222069486422257
1201	6	0.9112541192415264
1201	7	0.8180356256135255
1201	8	0.905308021142794
1201	9	1.0877173636292667
1201	10	1.4109161948028586
1201	11	1.0510050982544854
1201	12	1.1701051769665014
1201	13	1.1196848948672269
1201	14	1.9338643985151691
1201	15	1.4528247231491846
1201	16	1.648469739863259
1201	17	2.163172957167987
1201	18	2.6700862235740885
1201	19	1.8114044725499454
1201	20	1.5609662978037804
1201	21	1.4832440553745627
1201	22	1.7163259501238457
1201	23	3.3754368596431874
1201	24	2.246776232757931
1201	25	2.352881292825302
1202	1	0.9408011012241921
1202	2	1.329279378210444
1202	3	1.0909941678594528
1202	4	1.6173758046297522
1202	5	1.4652423532256265
1202	6	1.041194480817758
1202	7	0.847465067785194
1202	8	0.9627491842058546
1202	9	1.018928821541941
1202	10	1.5955778665645752
1202	11	1.17612312653835
1202	12	1.3006018646961763
1202	13	1.2010388158448535
1202	14	2.0666937705851893
1202	15	1.5490029803233867
1202	16	1.8710704329461478
1202	17	2.269970914855989
1202	18	2.8471953044686558
1202	19	2.020493203157379
1202	20	1.6516742854247894
1202	21	1.6056410927670939
1202	22	1.9390253252567504
1202	23	3.579048330041502
1202	24	2.220732706658069
1202	25	2.3495525213013977
1203	1	0.9037461238419198
1203	2	1.1684307010477077
1203	3	1.0055515011205962
1203	4	1.583129834117237
1203	5	1.2801132987613144
1203	6	0.8743035061220255
1203	7	0.8599152913842047
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2211	12	-0.5780020861287412
2211	13	-0.47127297733517537
2211	14	-0.17783090140721924
2211	15	-0.3610675983571099
2211	16	-0.5664347328814117
2211	17	0.00338770999454141
2211	18	-5.694399040612218
2211	19	-1.8374375549884547
2211	20	-1.0301439780665547
2211	21	-0.8215166958634157
2211	22	-0.44208607334389427
2211	23	-4.851097103726303
2211	24	-2.142968954672071
2211	25	-1.443160786948475
2212	1	0.14684796441391157
2212	2	0.3909762791930582
2212	3	0.17353385039913505
2212	4	0.5875950034203825
2212	5	0.5238024439285738
2212	6	0.07169334703091579
2212	7	0.019857358034627737
2212	8	-0.07056525268001224
2212	9	-0.23949899402873598
2212	10	-3.9202006670711
2212	11	-1.2588541977246877
2212	12	-0.4106604179946771
2212	13	-0.1650042680553696
2212	14	0.4988744206229825
2212	15	0.12065830912157338
2212	16	0.2733002137473548
2212	17	0.8951220864357268
2212	18	1.3895233467967003
2212	19	0.6069255538754419
2212	20	0.1545087525449702
2212	21	0.07330927114955443
2212	22	0.28524603576317276
2212	23	1.6244753897542736
2212	24	0.6580531904071236
2212	25	0.673380941558392
2213	1	-1.3810234521419211
2213	2	-3.915870952869522
2213	3	-1.7414007060389451
2213	4	-3.12849657198991
2213	5	-1.1420649784776131
2213	6	-0.9500584024812113
2213	7	-0.8201247428715135
2213	8	-0.7836579012072704
2213	9	-0.7454916686588362
2213	10	-0.6916277883416058
2213	11	-0.7905045190382793
2213	12	-0.7084213739388758
2213	13	-0.9902544203219676
2213	14	-6.186469867618719
2213	15	-2.7625109491847226
2213	16	-1.8958489163649688
2213	17	-0.7056098290573445
2213	18	0.0845882517284835
2213	19	-0.5472245913475291
2213	20	-0.7016148506420592
2213	21	-0.925227519360611
2213	22	-0.762202307534821
2213	23	-5.661193733894489
2213	24	-2.835341132431207
2213	25	-1.947848336718657
2214	1	0.0922704124073519
2214	2	0.2559054008374524
2214	3	0.050440622317417194
2214	4	0.4364819838115821
2214	5	0.1798550210095442
2214	6	-0.1199041873525033
2214	7	-0.23556785118950785
2214	8	-0.36821759749275174
2214	9	-0.19164097813541048
2214	10	0.2184912810989772
2214	11	-0.2859613619001564
2214	12	-0.26713124031354596
2214	13	-0.3188714122525269
2214	14	0.25082210342254996
2214	15	-0.01850954528035942
2214	16	0.48739974799271124
2214	17	0.6516361545000677
2214	18	1.1770141596136838
2214	19	0.14383711734111415
2214	20	0.03912179448725084
2214	21	-0.036933473513755644
2214	22	0.42323366707390164
2214	23	1.9488229474863275
2214	24	0.9246427050396164
2214	25	0.8123218065545146
2215	1	-2.4074696844471646
2215	2	-4.407665028356934
2215	3	-2.4904389993198173
2215	4	-3.8536495023007404
2215	5	-4.816527036044558
2215	6	-2.344580913229983
2215	7	-1.7163394815029704
2215	8	-2.0482446342569425
2215	9	-1.67191730161408
2215	10	-3.4341145969093034
2215	11	-2.1541983487481247
2215	12	-1.7650693926709167
2215	13	-1.7245687322968948
2215	14	-1.0249504223555377
2215	15	-1.6004308837938979
2215	16	-2.329097236200205
2215	17	-7.089071477156498
2215	18	-3.048675590288653
2215	19	-2.182014006110883
2215	20	-1.8544352853522952
2215	21	-1.9060976413308524
2215	22	-1.6515400025818892
2215	23	-6.251424022225319
2215	24	-3.601446461965576
2215	25	-3.3478696918303257
2216	1	-0.6739842777554372
2216	2	-0.21178465883012976
2216	3	-0.40110059338083914
2216	4	-2.698422919029454
2216	5	-1.0161814524453732
2216	6	-0.7681316628384209
2216	7	-0.7890613929181072
2216	8	-0.6612682098321159
2216	9	-0.7607445104847912
2216	10	-0.4194681214604472
2216	11	-0.978093731344913
2216	12	-1.6674982703755035
2216	13	-1.059523953956493
2216	14	-6.775638594520419
2216	15	-3.0454672053541003
2216	16	-1.3932211163913095
2216	17	-0.5290297697156348
2216	18	0.26077120844101387
2216	19	-0.2983442144329871
2216	20	-0.8277928933863237
2216	21	-1.010615961642948
2216	22	-0.5260853927561442
2216	23	0.6511762701118897
2216	24	-0.20442835752135835
2216	25	-0.16039491577078024
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	96427.0
3	3.0
4	863.0
5	5065.0
6	6094.0
7	5279.0
8	5142.0
9	4895.0
10	4597.0
11	4501.0
12	4405.0
13	4429.0
14	4572.0
15	4709.0
16	4829.0
17	5068.0
18	5825.0
19	6787.0
20	8289.0
21	10551.0
22	12941.0
23	15854.0
24	19136.0
25	22179.0
26	25837.0
27	29488.0
28	33326.0
29	40855.0
30	47700.0
31	54921.0
32	70804.0
33	93594.0
34	124138.0
35	165356.0
36	237176.0
37	336696.0
38	871611.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.77156558004666	17.88437999597708	11.840970117078625	25.503084306897634
2	14.18864249951939	19.743143958073873	41.39177024214512	24.676443300261617
3	19.13463422910079	24.472257172693308	28.810117886279084	27.58299071192682
4	10.785871667661857	16.110467382897294	39.193089353608194	33.91057159583265
5	12.48675338394627	37.75285575312653	36.36756058341942	13.392830279507779
6	32.80886577048016	36.149949341438706	18.018571324120785	13.02261356396035
7	28.822106656873274	30.990899751028955	21.846070799026176	18.34092279307159
8	25.960987655352657	35.93865489513631	20.29577850247248	17.80457894703856
9	27.058381106784108	14.892238137420403	19.332620213601643	38.716760542193846
10	14.084951397484252	28.942963186446285	35.0594170038185	21.91266841225096
11	34.96216114135351	22.450707956208618	23.443296444989826	19.143834457448047
12	23.29751484985943	24.629813696256083	31.474056145566852	20.598615308317633
13	29.089646597580483	21.37518946895216	25.266301014567595	24.26886291889977
14	21.000248599798198	21.199044875397185	28.272314965498946	29.52839155930568
15	24.42366315254668	29.05997011772413	22.964980245609578	23.55138648411961
16	22.63455160328805	27.714952072245524	27.329432596555353	22.321063727911074
17	22.273011776955673	27.68633336648118	27.86591668971695	22.174738166846193
18	21.893010029839672	26.95710557695184	29.40837065033103	21.741513742877462
19	23.63006758097871	26.889315201980335	27.518282549749724	21.962334667291234
20	23.888941580724428	26.748673732572176	27.462395090439383	21.899989596264014
21	23.446364746299814	26.462838523886685	27.15557620615914	22.935220523654365
22	23.18615289532308	26.994390837797305	26.844456590734247	22.97499967614537
23	21.284836998010658	26.648540710071188	29.016154999074484	23.050467292843674
24	23.004975781354798	27.156642043367484	27.20423938075822	22.634142794519494
25	22.98189357776084	26.391869576263254	27.252731089627876	23.373505756348028
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	223.0
1	223.0
2	364.5
3	506.0
4	506.0
5	506.0
6	950.5
7	1395.0
8	1395.0
9	1395.0
10	2208.0
11	3021.0
12	3021.0
13	3021.0
14	5641.0
15	8261.0
16	8261.0
17	8261.0
18	15763.5
19	23266.0
20	23266.0
21	23266.0
22	37708.5
23	52151.0
24	52151.0
25	52151.0
26	74995.5
27	97840.0
28	97840.0
29	97840.0
30	126495.0
31	155150.0
32	155150.0
33	155150.0
34	182438.5
35	209727.0
36	209727.0
37	209727.0
38	234199.0
39	258671.0
40	258671.0
41	258671.0
42	278540.0
43	298409.0
44	298409.0
45	298409.0
46	319194.0
47	339979.0
48	339979.0
49	339979.0
50	325890.0
51	311801.0
52	311801.0
53	311801.0
54	276900.5
55	242000.0
56	242000.0
57	242000.0
58	207568.0
59	173136.0
60	173136.0
61	173136.0
62	141689.0
63	110242.0
64	110242.0
65	110242.0
66	85595.0
67	60948.0
68	60948.0
69	60948.0
70	44625.5
71	28303.0
72	28303.0
73	28303.0
74	20082.0
75	11861.0
76	11861.0
77	11861.0
78	8243.0
79	4625.0
80	4625.0
81	4625.0
82	3142.5
83	1660.0
84	1660.0
85	1660.0
86	1112.5
87	565.0
88	565.0
89	565.0
90	356.5
91	148.0
92	148.0
93	148.0
94	91.5
95	35.0
96	35.0
97	35.0
98	27.0
99	19.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11040367728207283
2	0.04853918766620077
3	0.03646704890928853
4	0.031955661415355927
5	0.03667590944141504
6	0.019507573700615972
7	0.020510104254823216
8	0.02493794753590521
9	0.029324018710561908
10	0.035756923100058395
11	0.021011369531926838
12	0.020259471616271403
13	0.017126563634373763
14	0.0224316211503871
15	0.022974658533916028
16	0.022891114321065425
17	0.026065794409388367
18	0.0202176995098461
19	0.033960722523770415
20	0.023851872768847364
21	0.028572120794906477
22	0.037302491037794566
23	0.028780981327032985
24	0.03972527321046208
25	0.031663256670378816
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2393942.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.82769933031159
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.52198242141152	58.44842159084994
2	11.494749397969246	16.282933084732917
3	3.160343239755601	6.715195222979778
4	1.1759119736220227	3.3314855882645564
5	0.5445054314214341	1.9283033490219468
6	0.3030592396313525	1.2878993222329373
7	0.1820571863919457	0.9026284161083857
8	0.11633103389421526	0.6591567593155012
9	0.0849058610796949	0.5412318119940451
>10	0.3806289250600633	4.637680534153446
>50	0.022476242052685304	1.111806383913151
>100	0.010180164128079969	1.3902945642334374
>500	0.0017332838308791176	0.8218025363831818
>1k	0.0010160629353380417	1.2609184746622073
>5k	1.1953681592212254E-4	0.6802423611544689
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	8721	0.3642945401350576	No Hit
CCCATGTACTCTGCGTTGATACCAC	7348	0.30694143801311813	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3483	0.1454922466793264	No Hit
GTATCAACGCAGAGTACATGGGAAG	2473	0.10330241918977151	No Hit
GCGTTGATACCACTGCTTCCCATGT	2457	0.10263406548696669	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2403	0.10037837174000039	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.1772106425301866E-5	0.0
10	0.0	0.0	0.0	4.1772106425301866E-5	0.0
11	0.0	0.0	0.0	8.354421285060373E-5	0.0
12	0.0	0.0	0.0	8.354421285060373E-5	0.0
13	0.0	0.0	0.0	1.253163192759056E-4	4.1772106425301866E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGC	50	0.0014980589	13.302238	3
CGTAGAC	65	5.4462813E-5	13.15606	3
AACCGTC	60	4.1006453E-4	12.664034	7
CGGAACA	55	0.0030654112	12.092945	4
GTCCTAC	1100	0.0	11.8461895	1
GCGTTTA	210	0.0	11.760689	13
GTATTAA	370	0.0	11.311019	1
GTCTTAC	185	0.0	11.311019	1
CGTTTAT	210	0.0	11.308354	14
TAGGACC	580	0.0	11.303626	4
GACGTGA	390	0.0	11.202799	7
TAAGACG	60	0.005869873	11.085198	4
TAACCCG	60	0.00587444	11.08404	5
GTAGGAC	1015	0.0	11.046193	3
TCCTACA	1280	0.0	10.689746	2
TCTAATA	240	0.0	10.689745	2
TGCGTTT	245	0.0	10.467868	12
TTAGACT	155	3.6543497E-9	10.421109	4
CTAGGAC	375	0.0	10.388414	3
GTGTAGG	450	0.0	10.35701	1
>>END_MODULE