FastQCFastQC Report
Thu 26 May 2016
SRR1513075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513075_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2393942
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC102610.42862358403002243No Hit
CCCATGTACTCTGCGTTGATACCAC95600.39934133742588585No Hit
GAGTACATGGGAAGCAGTGGTATCA38010.15877577652257238No Hit
GTATCAACGCAGAGTACTTTTTTTT36790.15367957953868555No Hit
CATGTACTCTGCGTTGATACCACTG27540.11504038109528134No Hit
GTATCAACGCAGAGTACATGGGAAG25700.10735431351302581No Hit
GCGTTGATACCACTGCTTCCCATGT25240.10543279661746191No Hit
TATCAACGCAGAGTACTTTTTTTTT24680.10309355865764501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACA5200.011.1671164
ACCGCTC600.005937028711.0682688
ACGGCCC700.001500801610.84941315
CTTGGAC3700.010.5486453
GTAGGAC10150.010.5042863
GTCCTAC10950.010.3547381
ACCGTCC1201.5513524E-610.2776788
TTGGACT3150.010.2750254
CCTACAC4650.010.2360313
TTAAGCC2053.6379788E-1210.2160593
TAGGACC5450.010.1308544
CTACACT5050.09.9907724
CGCCCTC951.6712607E-49.98411410
GTCTTAC2201.8189894E-129.9611951
AGGACGT7750.09.9494225
TGTAGGA12200.09.91630652
GGACGTG7300.09.9107226
TAGACTG3050.09.6755675
TAGGACG7500.09.6464364
CTGTAGG12100.09.6068441
Produced by FastQC (version 0.11.5)