FastQCFastQC Report
Thu 26 May 2016
SRR1513074_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513074_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712750
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC59820.34926288133119254No Hit
CCCATGTACTCTGCGTTGATACCAC47530.27750693329440956No Hit
GTATCAACGCAGAGTACTTTTTTTT28320.1653481243614071No Hit
GAGTACATGGGAAGCAGTGGTATCA25760.1504014012552912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAG400.005241659514.2654151
TAGGACG6150.012.5136574
AGGACGT6400.012.1722315
GTGCGCC1052.7254464E-711.76018811
TAAGACT2750.011.7468274
GTAGGAC7300.011.7133553
GGACGTG6300.011.6100946
GCGTTTA1104.9762275E-711.22596113
TAGGACC3900.011.2064374
GTCCTAC8100.011.1540271
GTATTAG1451.195076E-911.1499791
TGTAGGA9000.010.8741262
TAATACT2450.010.8584124
TCCAACG1252.23994E-710.6418
TTAGGAC5200.010.5970823
GCCTTAC1355.5972123E-810.5669751
CCTATAC1355.6794306E-810.556483
GCGCCAC1002.401859E-510.4487813
TGGCGAG6400.010.39062618
GACGTGA3600.010.2898647
Produced by FastQC (version 0.11.5)