##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513074_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1712750 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.261718873157204 31.0 30.0 34.0 25.0 34.0 2 29.018552620055466 31.0 30.0 34.0 16.0 34.0 3 29.395709239527076 31.0 30.0 34.0 23.0 34.0 4 32.25697912713473 35.0 33.0 37.0 19.0 37.0 5 32.529275142314994 35.0 33.0 37.0 23.0 37.0 6 32.965334111808495 37.0 35.0 37.0 26.0 37.0 7 32.935175010947304 37.0 35.0 37.0 26.0 37.0 8 32.93592526638447 37.0 35.0 37.0 26.0 37.0 9 34.31540446650124 39.0 35.0 39.0 26.0 39.0 10 33.81291315136476 39.0 34.0 39.0 21.0 39.0 11 34.189118668807474 39.0 35.0 39.0 26.0 39.0 12 34.03852868194424 39.0 35.0 39.0 25.0 39.0 13 34.12191504889797 39.0 35.0 39.0 25.0 39.0 14 34.47403707487958 40.0 34.0 41.0 17.0 41.0 15 34.999292949934315 40.0 34.0 41.0 24.0 41.0 16 34.588594949642385 40.0 34.0 41.0 17.0 41.0 17 34.13063319223471 39.0 33.0 41.0 16.0 41.0 18 33.54208436724566 39.0 32.0 41.0 10.0 41.0 19 34.43867347832433 39.0 34.0 41.0 18.0 41.0 20 34.691333819880306 40.0 34.0 41.0 18.0 41.0 21 34.72862881331193 40.0 35.0 41.0 18.0 41.0 22 34.3269367975478 40.0 34.0 41.0 11.0 41.0 23 32.64840753174719 39.0 31.0 41.0 2.0 41.0 24 33.75924740913735 39.0 33.0 41.0 2.0 41.0 25 33.5000957524449 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7025858877408631 1101 2 0.992818976514517 1101 3 0.8411169712959463 1101 4 0.7261175409340481 1101 5 0.8971769254911592 1101 6 0.5863974362770676 1101 7 0.6130361060388765 1101 8 0.6600641999200647 1101 9 0.8260458858361517 1101 10 1.2061994284837496 1101 11 0.7985714391423997 1101 12 0.900043869218706 1101 13 0.8185091893393235 1101 14 -0.2564895311180422 1101 15 0.46110438439870194 1101 16 1.130577065224216 1101 17 1.8400046088024453 1101 18 -4.011972035426819 1101 19 -0.49675232015992066 1101 20 0.4385078251603787 1101 21 0.7699398417097996 1101 22 1.3236011035954078 1101 23 3.0644572802109806 1101 24 1.9832293503014213 1101 25 2.2375583253155966 1102 1 1.1378883101254615 1102 2 1.261157192882873 1102 3 1.1292834547311017 1102 4 1.6584607650026442 1102 5 1.5127243545816924 1102 6 1.0836402069182967 1102 7 1.1345914195455435 1102 8 1.0521325541244693 1102 9 1.1693157152331182 1102 10 1.5528318291630399 1102 11 1.2175381038129203 1102 12 1.303363730422248 1102 13 1.1631538200157792 1102 14 2.008386400622662 1102 15 1.5087710356234538 1102 16 1.819438095892984 1102 17 1.561616530111948 1102 18 1.86959817011261 1102 19 1.5518990356591047 1102 20 1.380022699679749 1102 21 1.477093610422294 1102 22 1.8389970700917289 1102 23 3.3237537841989493 1102 24 2.2160552095592863 1102 25 2.501271028852287 1103 1 0.4851018029483747 1103 2 0.8110214564818037 1103 3 0.6145459254265759 1103 4 1.033837557201302 1103 5 0.8539022624114025 1103 6 0.4619464841809702 1103 7 0.4504827220787959 1103 8 0.34825681138848097 1103 9 0.61759243507219 1103 10 0.8700536856257344 1103 11 0.45184522352020906 1103 12 0.5270698123306374 1103 13 0.3892849705109924 1103 14 1.268488135207285 1103 15 0.7335163536711704 1103 16 0.9954343944434214 1103 17 1.3725991002424252 1103 18 1.322876213872192 1103 19 0.7855884179271939 1103 20 0.7481939370506794 1103 21 0.6228876614587335 1103 22 0.9874801446386812 1103 23 2.4828483994632293 1103 24 1.3465810575179304 1103 25 1.6435811512886715 1104 1 0.636884525616253 1104 2 1.0308240660172494 1104 3 0.7678216413735193 1104 4 1.2966840334743708 1104 5 1.1665529578276335 1104 6 0.8101374822034515 1104 7 0.8059727983544889 1104 8 0.7133478691963901 1104 9 0.8163978762032542 1104 10 1.1755958277836598 1104 11 0.7775403112602532 1104 12 0.9789384354919122 1104 13 0.7925385132904239 1104 14 1.580683870075326 1104 15 1.054588746599407 1104 16 1.4272683289159573 1104 17 1.8425191454428358 1104 18 2.2790472527589998 1104 19 1.5465772754998994 1104 20 1.1179553209191226 1104 21 1.218520532933205 1104 22 1.4568228662553793 1104 23 2.937213557040536 1104 24 2.036784952111617 1104 25 2.044042517418582 1105 1 0.29513514899668536 1105 2 0.8288687701356352 1105 3 0.4125070176019463 1105 4 0.5984095342159037 1105 5 0.5914934718516562 1105 6 0.38785550237979294 1105 7 0.20583100986380742 1105 8 0.22934267028506383 1105 9 0.4607552646874211 1105 10 0.8116382176531189 1105 11 0.3797148088889628 1105 12 0.31879933609329214 1105 13 0.32994582110183046 1105 14 0.9779862218085711 1105 15 0.45483239463800373 1105 16 0.8522137321982797 1105 17 1.2741314598547433 1105 18 1.1734563858935019 1105 19 0.6625443707915437 1105 20 0.5074051163851507 1105 21 0.30836921038883247 1105 22 0.8411328289340432 1105 23 2.3821527672623475 1105 24 1.319069132101518 1105 25 1.2448309103193793 1106 1 -0.4259197889872972 1106 2 0.061231092352681316 1106 3 -0.1585042373626635 1106 4 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1107 16 -5.680225733174076 1107 17 -0.9486768201145921 1107 18 0.8048046524118178 1107 19 0.7277361284474395 1107 20 0.5971392739797707 1107 21 0.7422023545882013 1107 22 0.8763345908698099 1107 23 2.616806007096983 1107 24 1.6379821929609975 1107 25 1.4118005272743872 1108 1 0.24883395420587817 1108 2 0.5638377587801173 1108 3 0.35452545026349114 1108 4 0.8885426711828401 1108 5 0.7122775935988201 1108 6 0.3055314950791299 1108 7 0.1863412333951331 1108 8 0.350345510192966 1108 9 0.3867393935652075 1108 10 0.8115808984109947 1108 11 0.38656267566697977 1108 12 0.44111503347511416 1108 13 0.377599091795382 1108 14 0.94993517202996 1108 15 0.514355547909382 1108 16 0.7710389894149543 1108 17 0.9212591213256545 1108 18 0.7683323473277994 1108 19 0.6795982165972987 1108 20 0.5695238165822047 1108 21 0.6699454346963023 1108 22 1.0489747320425806 1108 23 2.7407307015481663 1108 24 1.4316173837960662 1108 25 1.7402881546899636 1109 1 0.0409646025112842 1109 2 0.40697417705102623 1109 3 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1.4020744052229972 1110 15 0.7802436106368589 1110 16 1.1801348848424382 1110 17 1.4772487494500837 1110 18 2.0684093806875268 1110 19 1.2574387472498856 1110 20 0.8874689869237145 1110 21 0.8624818393240474 1110 22 1.101403598750224 1110 23 2.47684837731844 1110 24 1.539495913780705 1110 25 2.0095085061235167 1111 1 0.5201191674292112 1111 2 0.8559390971933141 1111 3 0.6584810227323672 1111 4 1.2584345334678346 1111 5 0.8696728516852303 1111 6 0.3623263424797898 1111 7 0.28092846342205036 1111 8 0.07662906813496306 1111 9 0.2919368669789719 1111 10 0.5275874139502932 1111 11 0.49361428414949415 1111 12 0.5045552030287155 1111 13 0.4608985129418457 1111 14 -0.07203690045925981 1111 15 0.19064905953649713 1111 16 0.6999287080831138 1111 17 1.171998498653906 1111 18 1.8523752702576814 1111 19 1.0366803460480014 1111 20 0.7145303112242658 1111 21 0.5911501935476622 1111 22 0.8524892627730765 1111 23 0.8148551347105482 1111 24 0.6894675967829329 1111 25 0.10219589667724449 1112 1 0.5374199015072278 1112 2 0.8374418645141013 1112 3 0.6315571441070631 1112 4 1.204709686403028 1112 5 0.8391834863492278 1112 6 0.28895510024386795 1112 7 0.2939576251936771 1112 8 0.3518616698639505 1112 9 0.38822997216772137 1112 10 0.7043145271460673 1112 11 0.40116809043649226 1112 12 0.44161400184288624 1112 13 0.27015159006177925 1112 14 0.722368462517295 1112 15 0.5161935339256161 1112 16 0.9187553564301183 1112 17 1.082743604482168 1112 18 1.2914097718698372 1112 19 0.8503264050918133 1112 20 0.716184483158834 1112 21 0.7176344602253835 1112 22 0.8282047473829692 1112 23 2.3041573104254596 1112 24 1.247841750121367 1112 25 1.2380207918148045 1113 1 0.5087231947122071 1113 2 0.8205950186460917 1113 3 0.5734057555501622 1113 4 0.7544631849579702 1113 5 0.7871054044700045 1113 6 0.37974966335909954 1113 7 0.392605194675852 1113 8 0.36535854072163687 1113 9 0.532015826923363 1113 10 0.8379537576812837 1113 11 0.5579606652425539 1113 12 0.44594563660244546 1113 13 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-0.44646016265448907 2216 25 -0.2676311931657196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 81021.0 3 1.0 4 702.0 5 4073.0 6 4965.0 7 4498.0 8 4018.0 9 3820.0 10 3746.0 11 3520.0 12 3449.0 13 3434.0 14 3434.0 15 3485.0 16 3793.0 17 3850.0 18 4308.0 19 5199.0 20 6322.0 21 7868.0 22 9868.0 23 12055.0 24 14504.0 25 16602.0 26 19075.0 27 22206.0 28 24655.0 29 29753.0 30 35098.0 31 40183.0 32 50938.0 33 68168.0 34 89194.0 35 118217.0 36 169641.0 37 239981.0 38 597106.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.516494063212726 18.336179858293324 12.206214659545093 24.941111418948854 2 14.035939351030793 19.985174229094962 41.903890684181874 24.074995735692372 3 19.081189941615015 24.750771574291917 29.229640601746176 26.938397882346894 4 10.6382158551714 16.475152848935956 39.67034391886683 33.216287377025814 5 12.202813275182422 38.15046694200153 36.57000742364481 13.076712359171239 6 31.998843721348525 36.99139798760797 18.23556549617786 12.774192794865655 7 28.217156572189406 31.39759577903592 22.13740324280763 18.247844405967044 8 25.424597886150185 36.66527285108093 20.416556193154808 17.49357306961408 9 26.704098807770343 14.986561573224533 19.688258868135218 38.62108075086991 10 13.815137005423678 29.43049619127609 35.316758969279064 21.437607834021176 11 34.62571427737175 22.730386388654487 23.722622408965222 18.92127692500854 12 22.772363378141552 25.202915892172957 31.83935613512893 20.185364594556557 13 28.77388680091471 21.73485738578366 25.558205427071822 23.933050386229805 14 20.686785608126222 21.721016851954264 28.598391831733906 28.99380570818561 15 23.97736669734586 29.589498371822486 23.38694794971104 23.046186981120616 16 22.126727432421067 28.158550031301683 27.804537342440412 21.91018519383684 17 21.83383033322938 28.08896239737332 28.196830647431426 21.880376621965876 18 21.461541066971577 27.390594943465246 29.863793347107393 21.284070642455784 19 23.083931800447623 27.294013615613565 28.009604287469802 21.612450296469003 20 23.290265612827344 27.245258248644255 28.0991764495644 21.365299688964004 21 23.066999170686696 26.827584595797365 27.76628549402544 22.3391307394905 22 22.591740707945995 27.464680966387633 27.516021185579415 22.427557140086957 23 21.329252908470774 27.025358594589544 29.260442461337195 22.384946035602486 24 22.456849245858397 27.625360745619798 27.8086466804394 22.109143328082407 25 22.602661321596607 26.864350158156135 27.75178830906092 22.781200211186334 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 229.0 1 229.0 2 357.0 3 485.0 4 485.0 5 485.0 6 1053.0 7 1621.0 8 1621.0 9 1621.0 10 2570.0 11 3519.0 12 3519.0 13 3519.0 14 6018.5 15 8518.0 16 8518.0 17 8518.0 18 14962.0 19 21406.0 20 21406.0 21 21406.0 22 32712.5 23 44019.0 24 44019.0 25 44019.0 26 61275.5 27 78532.0 28 78532.0 29 78532.0 30 100003.0 31 121474.0 32 121474.0 33 121474.0 34 138996.5 35 156519.0 36 156519.0 37 156519.0 38 172283.0 39 188047.0 40 188047.0 41 188047.0 42 200877.5 43 213708.0 44 213708.0 45 213708.0 46 226381.5 47 239055.0 48 239055.0 49 239055.0 50 227429.0 51 215803.0 52 215803.0 53 215803.0 54 190004.0 55 164205.0 56 164205.0 57 164205.0 58 139558.5 59 114912.0 60 114912.0 61 114912.0 62 93620.5 63 72329.0 64 72329.0 65 72329.0 66 55613.5 67 38898.0 68 38898.0 69 38898.0 70 28527.5 71 18157.0 72 18157.0 73 18157.0 74 12677.5 75 7198.0 76 7198.0 77 7198.0 78 4969.5 79 2741.0 80 2741.0 81 2741.0 82 1833.5 83 926.0 84 926.0 85 926.0 86 630.0 87 334.0 88 334.0 89 334.0 90 208.5 91 83.0 92 83.0 93 83.0 94 51.0 95 19.0 96 19.0 97 19.0 98 16.0 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11046562545613779 2 0.050561961757407674 3 0.03888483433075464 4 0.03514815355422566 5 0.03812582104802219 6 0.02101882936797548 7 0.0201430448109765 8 0.02475551014450445 9 0.02895927601809955 10 0.036666180119690556 11 0.020726901182309153 12 0.019909502262443438 13 0.017398919865713035 14 0.021427528827908333 15 0.023704568676105677 16 0.022303313384907313 17 0.02779156327543424 18 0.020551744270909358 19 0.0349146110056926 20 0.02382133995037221 21 0.02860896219529996 22 0.03748357903955627 23 0.02977667493796526 24 0.039176762516420965 25 0.030243760035031382 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1712750.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.90490153670676 #Duplication Level Percentage of deduplicated Percentage of total 1 87.57649107296281 68.22637913969447 2 8.828336613923385 13.75541389281209 3 1.9533647913216279 4.565300751995435 4 0.6599233319745405 2.056450487970081 5 0.3175386337030047 1.236890799636649 6 0.17529386518313825 0.8193750784248681 7 0.1039659877711192 0.5669622028332851 8 0.07152745792430941 0.4457871653411409 9 0.045822457285669325 0.32128146207090186 >10 0.2418614036166419 3.2300189451374286 >50 0.014179081667375347 0.7565151625518781 >100 0.009189576433493022 1.4929189432973757 >500 0.0014426908614351918 0.7631463617594235 >1k 9.871042736135515E-4 1.409700096666058 >5k 7.59310979702732E-5 0.35385950980891945 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 5982 0.34926288133119254 No Hit CCCATGTACTCTGCGTTGATACCAC 4753 0.27750693329440956 No Hit GTATCAACGCAGAGTACTTTTTTTT 2832 0.1653481243614071 No Hit GAGTACATGGGAAGCAGTGGTATCA 2576 0.1504014012552912 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.838563713326522E-5 2 0.0 0.0 0.0 0.0 5.838563713326522E-5 3 0.0 0.0 0.0 0.0 5.838563713326522E-5 4 0.0 0.0 0.0 0.0 5.838563713326522E-5 5 0.0 0.0 0.0 0.0 5.838563713326522E-5 6 0.0 0.0 0.0 0.0 5.838563713326522E-5 7 0.0 0.0 0.0 0.0 5.838563713326522E-5 8 0.0 0.0 0.0 0.0 5.838563713326522E-5 9 0.0 0.0 0.0 5.838563713326522E-5 5.838563713326522E-5 10 0.0 0.0 0.0 5.838563713326522E-5 5.838563713326522E-5 11 0.0 0.0 0.0 5.838563713326522E-5 5.838563713326522E-5 12 0.0 0.0 0.0 1.1677127426653044E-4 1.1677127426653044E-4 13 0.0 0.0 0.0 1.1677127426653044E-4 1.7515691139979565E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGTAG 40 0.0052416595 14.265415 1 TAGGACG 615 0.0 12.513657 4 AGGACGT 640 0.0 12.172231 5 GTGCGCC 105 2.7254464E-7 11.760188 11 TAAGACT 275 0.0 11.746827 4 GTAGGAC 730 0.0 11.713355 3 GGACGTG 630 0.0 11.610094 6 GCGTTTA 110 4.9762275E-7 11.225961 13 TAGGACC 390 0.0 11.206437 4 GTCCTAC 810 0.0 11.154027 1 GTATTAG 145 1.195076E-9 11.149979 1 TGTAGGA 900 0.0 10.874126 2 TAATACT 245 0.0 10.858412 4 TCCAACG 125 2.23994E-7 10.64 18 TTAGGAC 520 0.0 10.597082 3 GCCTTAC 135 5.5972123E-8 10.566975 1 CCTATAC 135 5.6794306E-8 10.55648 3 GCGCCAC 100 2.401859E-5 10.44878 13 TGGCGAG 640 0.0 10.390626 18 GACGTGA 360 0.0 10.289864 7 >>END_MODULE