FastQCFastQC Report
Thu 26 May 2016
SRR1513074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513074_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712750
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC70160.4096336301269887No Hit
CCCATGTACTCTGCGTTGATACCAC62670.36590278791417313No Hit
GTATCAACGCAGAGTACTTTTTTTT41890.24457743395124798No Hit
TATCAACGCAGAGTACTTTTTTTTT29430.17182893008319955No Hit
GAGTACATGGGAAGCAGTGGTATCA27160.15857539045394833No Hit
GGTATCAACGCAGAGTACTTTTTTT27020.1577579915340826No Hit
GTATCAACGCAGAGTACATGGGAAG18430.10760472923660779No Hit
CATGTACTCTGCGTTGATACCACTG18170.1060867026711429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4500.011.6336864
GTAGGAC8400.011.2181993
TCCAACG1105.0284325E-711.21689918
GTAAGAC1701.0913936E-1111.1982023
GGACGTG6250.010.965286
CTGGACG700.00146755710.8782534
TAGGACG6600.010.8164444
ATAGGAC1855.456968E-1210.8047513
CCGTCCA803.8157817E-410.67388259
AGGACGT6600.010.6722255
TGTAGGA8800.010.606332
GCCGTCC909.639231E-510.5421078
GACGTGG3350.010.4794747
GAACAGT3000.010.4703186
CCTACAC2650.010.416443
GTCCTAC5800.010.3475171
TTAGGAC5250.010.334343
CCTGGAC3050.010.2986743
TAGGACA4450.010.2671164
AGGACGC750.002605474810.1530356
Produced by FastQC (version 0.11.5)